巨噬细胞样细胞系:内源性过氧化物活性、细胞表面抗原和集落刺激因子的产生。

H Van Loveren, J Hilgers, J M De Bakker, R A De Weger, P Brederoo, W Den Otter
{"title":"巨噬细胞样细胞系:内源性过氧化物活性、细胞表面抗原和集落刺激因子的产生。","authors":"H Van Loveren,&nbsp;J Hilgers,&nbsp;J M De Bakker,&nbsp;R A De Weger,&nbsp;P Brederoo,&nbsp;W Den Otter","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The murine macrophage-like cells NCTC 1469, J774A, WEHI-3, IC21, and P388D1, were compared with respect to their peroxidatic activity, display of cell surface antigens, and production of colony-stimulating factor. Peroxidatic activity was demonstrated in the nuclear envelope and in the cisternae of the rough endoplasmic reticulum of NCTC 1469 cells, J774A cells, and P388D1 cells, and in granules of WEHI-3 cells and IC21 cells. Colony-stimulating factor was produced only by WEHI-3 cells. NCTC 1469 and IC21 cells had a weak expression of M1/69 and Mac-1 antigens, whereas P388D1 cells expressed these antigens at a high level. WEHI-3 cells expressed M1/69 at a high level and Mac-1 at a low level, whereas J774A cells had an opposite expression T 200, Pgp-1, and ThB antigens were expressed at different levels by the various cell lines, without overt correlation among themselves or with the expression of M1/69 or Mac-1 antigens. These data suggest that macrophage-like cell lines cannot be placed in a maturation/differentiation lineage.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 3","pages":"221-9"},"PeriodicalIF":0.0000,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Macrophage-like cell lines: endogenous peroxidatic activity, cell surface antigens, and colony-stimulating factor production.\",\"authors\":\"H Van Loveren,&nbsp;J Hilgers,&nbsp;J M De Bakker,&nbsp;R A De Weger,&nbsp;P Brederoo,&nbsp;W Den Otter\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The murine macrophage-like cells NCTC 1469, J774A, WEHI-3, IC21, and P388D1, were compared with respect to their peroxidatic activity, display of cell surface antigens, and production of colony-stimulating factor. Peroxidatic activity was demonstrated in the nuclear envelope and in the cisternae of the rough endoplasmic reticulum of NCTC 1469 cells, J774A cells, and P388D1 cells, and in granules of WEHI-3 cells and IC21 cells. Colony-stimulating factor was produced only by WEHI-3 cells. NCTC 1469 and IC21 cells had a weak expression of M1/69 and Mac-1 antigens, whereas P388D1 cells expressed these antigens at a high level. WEHI-3 cells expressed M1/69 at a high level and Mac-1 at a low level, whereas J774A cells had an opposite expression T 200, Pgp-1, and ThB antigens were expressed at different levels by the various cell lines, without overt correlation among themselves or with the expression of M1/69 or Mac-1 antigens. These data suggest that macrophage-like cell lines cannot be placed in a maturation/differentiation lineage.</p>\",\"PeriodicalId\":17481,\"journal\":{\"name\":\"Journal of the Reticuloendothelial Society\",\"volume\":\"33 3\",\"pages\":\"221-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1983-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Reticuloendothelial Society\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Reticuloendothelial Society","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

比较小鼠巨噬细胞样细胞NCTC 1469、J774A、WEHI-3、IC21和P388D1的过氧化物活性、细胞表面抗原的显示和集落刺激因子的产生。在NCTC 1469细胞、J774A细胞和P388D1细胞的粗内质网核膜和池池中,以及在WEHI-3细胞和IC21细胞的颗粒中,均表现出过氧化物活性。集落刺激因子仅由WEHI-3细胞产生。NCTC 1469和IC21细胞弱表达M1/69和Mac-1抗原,而P388D1细胞高表达这些抗原。WEHI-3细胞高水平表达M1/69,低水平表达Mac-1,而J774A细胞则相反,不同细胞系中T 200、Pgp-1和ThB抗原的表达水平不同,它们之间以及与M1/69或Mac-1抗原的表达均无明显相关性。这些数据表明,巨噬细胞样细胞系不能被置于成熟/分化谱系中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Macrophage-like cell lines: endogenous peroxidatic activity, cell surface antigens, and colony-stimulating factor production.

The murine macrophage-like cells NCTC 1469, J774A, WEHI-3, IC21, and P388D1, were compared with respect to their peroxidatic activity, display of cell surface antigens, and production of colony-stimulating factor. Peroxidatic activity was demonstrated in the nuclear envelope and in the cisternae of the rough endoplasmic reticulum of NCTC 1469 cells, J774A cells, and P388D1 cells, and in granules of WEHI-3 cells and IC21 cells. Colony-stimulating factor was produced only by WEHI-3 cells. NCTC 1469 and IC21 cells had a weak expression of M1/69 and Mac-1 antigens, whereas P388D1 cells expressed these antigens at a high level. WEHI-3 cells expressed M1/69 at a high level and Mac-1 at a low level, whereas J774A cells had an opposite expression T 200, Pgp-1, and ThB antigens were expressed at different levels by the various cell lines, without overt correlation among themselves or with the expression of M1/69 or Mac-1 antigens. These data suggest that macrophage-like cell lines cannot be placed in a maturation/differentiation lineage.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信