Journal of the Reticuloendothelial Society最新文献_第10页

Interactions between fresh human blood monocytes and tumor targets at the single cell level: binding and morphological characteristics. 新鲜人血单核细胞与肿瘤靶点在单细胞水平上的相互作用:结合和形态特征。

Journal of the Reticuloendothelial Society Pub Date : 1982-11-01

K E Muse, H S Koren

{"title":"Interactions between fresh human blood monocytes and tumor targets at the single cell level: binding and morphological characteristics.","authors":"K E Muse, H S Koren","doi":"","DOIUrl":"","url":null,"abstract":"Highly purified (97-99%), human peripheral blood monocytes, isolated by an EDTA-reversible adherence procedure, exhibit spontaneous killing in a rapid, 51Cr-release assay. Using the single cell conjugate/dextran assay, we have quantitatively determined select requirements for the adhesion of monocytes to neoplastic target cells. Monocytes expressed a broad binding capacity to a wide spectrum of nonadherent tumor cell lines, while binding at minimal levels to nontumor target cells. Binding of monocytes to K562 tumor cells is an extremely rapid event, reaching saturation levels within 5 min after cosedimentation. The binding of monocytes to K562 targets was reduced by low temperatures and pretreatment with metabolic inhibitors. Pretreatment of monocytes with cytoskeletal-disruptive drugs significantly reduced binding. Monocytes were shown to exhibit a specific, Mg2+-dependent, Ca2+-independent mechanism in their binding to K562 cells. Subsequent to binding, Ca2+ was required for monocyte-mediated target cell lysis to proceed. The binding of tumor cells by monocytes was studied morphologically using transmission (TEM) and scanning electron microscopic (SEM) techniques. It was observed that binding was achieved initially through complex, surface membrane interdigitations from apposing effector--target cells and progressed to stable conjugates, which were characterized by contact regions consisting of large expanses of closely apposed surface membranes. The application of the single cell conjugate/dextran assay to assess monocyte-binding requirements and the morphological characterization of the monocyte--target cell conjugation process may help in clarifying the mechanisms of monocyte-mediated tumor cell lysis.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"32 5","pages":"323-37"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18009298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture of human bone marrow in the teflon culture bag: identification of the human monoblast. 聚四氟乙烯培养袋培养人骨髓:人单细胞的鉴定。

Journal of the Reticuloendothelial Society Pub Date : 1982-11-01

J W van der Meer, J S van de Gevel, R H Beelen, D Fluitsma, E C Hoefsmit, R van Furth

引用次数: 0

Combined autoradiography and reticulin staining by silver impregnation or T-B lymphocyte staining by immunoperoxidase techniques in the study of migration of labeled lymphocytes. 结合放射自显影和银浸渍网蛋白染色或免疫过氧化物酶T-B淋巴细胞染色技术研究标记淋巴细胞的迁移。

Journal of the Reticuloendothelial Society Pub Date : 1982-11-01

N van Rooijen, C D Dijkstra, F J van Schooten

引用次数: 0

Human peripheral blood monocyte and bronchoalveolar macrophage cytotoxicity for cultured human lung tumor cells. 人外周血单核细胞和支气管肺泡巨噬细胞对培养的人肺肿瘤细胞的细胞毒性。

Journal of the Reticuloendothelial Society Pub Date : 1982-11-01

S Swinburne, P Cole

引用次数: 0

Functional evaluation of lung macrophages from cigarette smokers and nonsmokers. 吸烟者和非吸烟者肺巨噬细胞的功能评价。

Journal of the Reticuloendothelial Society Pub Date : 1982-10-01

G L Fisher, K L McNeill, G L Finch, F D Wilson, D W Golde

引用次数: 0

Flow cytometric analysis of macrophage heterogeneity and differentiation: utilization of electronic cell volume and fluorescent substrates corresponding to common macrophage markers. 巨噬细胞异质性和分化的流式细胞分析:利用电子细胞体积和与常见巨噬细胞标志物对应的荧光底物。

Journal of the Reticuloendothelial Society Pub Date : 1982-10-01

S Haskill, S Becker

{"title":"Flow cytometric analysis of macrophage heterogeneity and differentiation: utilization of electronic cell volume and fluorescent substrates corresponding to common macrophage markers.","authors":"S Haskill, S Becker","doi":"","DOIUrl":"","url":null,"abstract":"We have utilized electronic cell volume (ECV) and several fluorescent analogues of traditional macrophage markers in a flow cytometric characterization of macrophage heterogeneity. With this technique, various features of macrophage differentiation and activation can be determined on as few as 10,000 cells per assay. Quantitations of cell volume, phagocytosis, RNA, myeloperoxidase, acid phosphatase, and the ectoenzyme leucine aminopeptidase have been carried out on cultured human monocytes and peritoneal macrophages. Quantitations of the amount of a particular marker on a per cell basis, as well as population distributions, are of particular value in characterizing the heterogeneity in resident and inflammatory macrophages and the temporal relationships between different markers during macrophage activation and differentiation. This technique will facilitate a sophisticated analysis of monocyte-macrophage development by permitting the simultaneous analysis of a number of these features. In this way the interrelationships between different macrophage markers can be quantitated at the single cell level.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"32 4","pages":"273-85"},"PeriodicalIF":0.0,"publicationDate":"1982-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17250970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contact with specific surfaces stimulates the production of the second component of complement (C2) in human peripheral blood monocytes via a lymphocyte factor. 与特定表面的接触通过淋巴细胞因子刺激人外周血单核细胞补体第二组分(C2)的产生。

Journal of the Reticuloendothelial Society Pub Date : 1982-10-01

R C Strunk, K S Kunke, J R Hollister, R A Musson

{"title":"Contact with specific surfaces stimulates the production of the second component of complement (C2) in human peripheral blood monocytes via a lymphocyte factor.","authors":"R C Strunk, K S Kunke, J R Hollister, R A Musson","doi":"","DOIUrl":"","url":null,"abstract":"Whole mononuclear cells plated on surfaces coated with the polymer, poly (2-hydroxyethyl methacrylate) (poly-HEMA) produced significantly less C2 when compared to production by cells on tissue culture plastic dishes. The reduction in C2 production was dependent on the amount of poly-HEMA used to coat the dishes and was not due to nonspecific damage of the cells or effects of the poly-HEMA on the hemolytic activity of C2. T and B lymphocytes, but not monocytes, plated on tissue culture plastic produced a soluble factor that increased the production of C2 in freshly adherent monocytes. Lymphocytes plated on the poly-HEMA surface did not produce this soluble factor, which was termed surface-dependent factor (SDF). Whole mononuclear cells plated on poly-HEMA were able to respond to SDF by increasing C2 production by the same percentage as cells on the tissue culture plastic. This suggested that the primary basis for the decreased production of C2 by monocytes in the whole mononuclear cells plated on the poly-HEMA was decreased production of SDF by the lymphocytes. The effect of the poly-HEMA surface on C2 production was probably related to a generalized alteration in maturation of monocytes into macrophages, for SDF had the same type of effect on beta-glucosaminidase levels in monocytes as seen with C2, except that the magnitude of the effect was less. These studies suggest that interaction of lymphocytes with surfaces may modulate the function of the lymphocytes. In addition, interaction of lymphocytes with surfaces and the production of SDF in vivo may be responsible for enhancing maturation of monocytes in tissues.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"32 4","pages":"297-309"},"PeriodicalIF":0.0,"publicationDate":"1982-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18034303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accessory function of aging Kupffer cells with different lymphocyte populations. 不同淋巴细胞群衰老Kupffer细胞的辅助功能。

Journal of the Reticuloendothelial Society Pub Date : 1982-10-01

M F Heil, J S Garvey

引用次数: 0

Endocytosis of heat-denatured albumin by cultured rat Kupffer cells. 热变性白蛋白在培养大鼠Kupffer细胞中的内吞作用。

Journal of the Reticuloendothelial Society Pub Date : 1982-10-01

A Brouwer, D L Knook

{"title":"Endocytosis of heat-denatured albumin by cultured rat Kupffer cells.","authors":"A Brouwer, D L Knook","doi":"","DOIUrl":"","url":null,"abstract":"Purified Kupffer cells were obtained by centrifugal elutriation of sinusoidal cells isolated by pronase treatment of the rat liver. The endocytosis of radioactively labeled heat-aggregated colloidal albumin (CA 125I) was investigated in maintenance cultures of the purified Kupffer cells. The endocytic capacity of the cells was studied during 4 days of culture. Maximum uptake was observed after 24 hr of culture, with a gradual decline during the following days. When the uptake was measured after incubation with increasing concentrations of CA 125I, a saturation effect was observed. This finding and the observed high rate of uptake are strong indications that receptor sites on the cell membrane are involved in the mechanism of endocytosis. The uptake of CA 125I by Kupffer cells was inhibited by the metabolic inhibitors fluoride and antimycin A, indicating that endocytosis of CA 125I is dependent on energy derived from both glycolysis and mitochondrial respiration. The mechanism of internalization may also require the action of microfilaments as well as intact microtubules, since both cytochalasin B and colchicine inhibited the uptake of CA 125I. The intracellular degradation of CA 125I by Kupffer cells was strongly inhibited by chloroquine but not by colchicine. The degradation of ingested CA 125I occurred within the Kupffer cell lysosomes.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"32 4","pages":"259-68"},"PeriodicalIF":0.0,"publicationDate":"1982-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18175311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intravital microscopy of the perfused liver: sinusoidal cells trap xenogeneic erythrocytes and tumor cells. 灌注肝脏的活体显微镜:窦状细胞捕获异种红细胞和肿瘤细胞。

Journal of the Reticuloendothelial Society Pub Date : 1982-10-01

V Burkart, E A Friedrich

引用次数: 0