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Journal of the Reticuloendothelial Society最新文献

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Heterogeneity of human peritoneal macrophages: cytochemical and flow cytometric studies. 人腹膜巨噬细胞的异质性:细胞化学和流式细胞术研究。
Journal of the Reticuloendothelial Society Pub Date : 1983-02-01
S Becker, J Halme, S Haskill
{"title":"Heterogeneity of human peritoneal macrophages: cytochemical and flow cytometric studies.","authors":"S Becker,&nbsp;J Halme,&nbsp;S Haskill","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human peritoneal macrophages obtained from females undergoing laparoscopic examination have been investigated. Morphologic heterogeneity as well as a wide variation in the content of nonspecific esterase, acid phosphatase (AP), and myeloperoxidase (MP) were observed with standard cytochemical stains. This heterogeneity was evident within the same sample as well as between different individuals. Phagocytic activity was also highly variable. In only 3 cases out of 85 samples screened could human peritoneal macrophages be characterized as resident (peroxidase negative, acid phosphatase negative) by the criteria used in rodent systems, thus indicating that female peritoneal macrophages are continuously responding to stimuli/irritation. A newly developed flow cytofluorometric method was used to quantitatively document the macrophage heterogeneity in enzyme expression and phagocytic activity. Population distributions derived from single-cell analysis of various enzyme activities and phagocytic levels further emphasized the spread in expression of these parameters both within the individual peritoneal samples as well as between donors. Simultaneous determination of two markers such as leucine aminopeptidase (LAP) and zymosan ingestion indicated that the association between these features varied markedly between individuals. It is hoped that the use of this multiparameter flow cytometric technique on in vitro differentiating blood monocytes, subjected to different stimuli, will give us a better understanding not only of the development of monocytes to macrophages but also of the sources of heterogeneity in normal human resident macrophages.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 2","pages":"127-38"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17250971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human macrophage-like cell line U937-1 elaborates mitogenic activity for fibroblasts. 人巨噬细胞样细胞系U937-1阐述了成纤维细胞的有丝分裂活性。
Journal of the Reticuloendothelial Society Pub Date : 1983-02-01
W Wharton
{"title":"Human macrophage-like cell line U937-1 elaborates mitogenic activity for fibroblasts.","authors":"W Wharton","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The human macrophage-like cell line U937-1 was shown to elaborate mitogenic activity for cultured fibroblasts. Activity was present in the culture medium when cells were maintained either in the presence or absence of serum components, although maximal levels were observed when the macrophage-like cells were incubated in medium supplemented with platelet-poor plasma. The mitogenic activity acted synergistically with platelet-poor plasma to allow density-arrested cells to initiate DNA synthesis and its action was potentiated by the addition of cholera toxin. The elaborated activity bound to DEAE Sephacel and was eluted in a single peak using an NaCl gradient.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 2","pages":"151-6"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17879291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative studies on antibody and lectin-dependent macrophage-mediated tumor lysis. 抗体和凝集素依赖性巨噬细胞介导肿瘤溶解的比较研究。
Journal of the Reticuloendothelial Society Pub Date : 1983-02-01
M Esumi-Kurisu, T Okutomi, D Mizuno, M Yamazaki
{"title":"Comparative studies on antibody and lectin-dependent macrophage-mediated tumor lysis.","authors":"M Esumi-Kurisu,&nbsp;T Okutomi,&nbsp;D Mizuno,&nbsp;M Yamazaki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three characteristics of the mechanisms of antibody-dependent macrophage-mediated cytolysis (ADMC) and lectin-dependent macrophage-mediated cytolysis (LDMC) in a C3H/He mouse-MM46 syngeneic tumor system were compared: the role of divalent cations, the effect of cyclic nucleotides, and the role of protease and oxygen compounds as lytic substances. Mg++, but not Ca++, was required for ADMC but neither was required for LDMC. Ca++ inhibited both LDMC and ADMC. Dibutyryl cyclic adenosine 3',5'-monophosphate (cAMP) and its agonists, such as cholera toxin, prostaglandin E1 (PGE1) and E2 (PGE2), inhibited ADMC, but had no effect on LDMC. Dibutyryl cyclic guanosine 3',5'-monophosphate (cGMP) had no effect on either ADMC or LDMC. Isoproterenol and isobutylmethylxanthine had no effect on either ADMC or LDMC. Protease inhibitors, such as pepstatin, bestatin, chymostatin, elastatinal, leupeptin, and bovine pancreatic trypsin inhibitor, did not affect either ADMC or LDMC. Tosylphenylalanyl-chloromethylketone inhibited both ADMC and LDMC, and also inhibited macrophage-tumor cell contact in both systems. Neither catalase nor superoxide dismutase clearly inhibited either ADMC or LDMC. The different sensitivities of ADMC and LDMC to pharmacological agents suggest that these two types of mediator-dependent macrophage-mediated cytolysis have partly different cytolytic processes as well as different recognition sites.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 2","pages":"83-92"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17462302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analytical subcellular fractionation of guinea pig peritoneal macrophages: preparation of purified plasma membranes. 豚鼠腹膜巨噬细胞的亚细胞分离:纯化质膜的制备。
Journal of the Reticuloendothelial Society Pub Date : 1983-02-01
G Chauvet, M Auclair, M Vial, A Anteunis
{"title":"Analytical subcellular fractionation of guinea pig peritoneal macrophages: preparation of purified plasma membranes.","authors":"G Chauvet,&nbsp;M Auclair,&nbsp;M Vial,&nbsp;A Anteunis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method using sucrose gradient centrifugation is described for the purification of plasma membranes of guinea pig peritoneal macrophages. The subcellular fractions obtained have been submitted to a biochemical and ultrastructural analysis. Two plasma membrane markers, 5'-nucleotidase and alkaline phosphodiesterase I, have been assayed at the same time as markers for other subcellular organelles, DNA (nuclei), succinic dehydrogenase (mitochondria), inosine diphosphatase (endoplasmic reticulum), and acid phosphatase (lysosomes). The exposure of the plasma membranes to a low concentration of digitonin allowed us to obtain their high purification. They are only contaminated by 2-3% of other cell components present in the macrophages homogenate. The representative ultrastructural technique used has confirmed the purity of the plasma membranes isolated.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 2","pages":"139-49"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17359418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional activities of the NCTC 1469 macrophage-like cell line: comparison of the NCTC 1469 cell line with various other macrophage-like cell lines. NCTC 1469巨噬细胞样细胞株的功能活性:NCTC 1469细胞株与其他各种巨噬细胞样细胞株的比较。
Journal of the Reticuloendothelial Society Pub Date : 1983-01-01
R A De Weger, H Van Loveren, C D Van Basten, R Oskam, B A Van Der Zeijst, W Den Otter
{"title":"Functional activities of the NCTC 1469 macrophage-like cell line: comparison of the NCTC 1469 cell line with various other macrophage-like cell lines.","authors":"R A De Weger,&nbsp;H Van Loveren,&nbsp;C D Van Basten,&nbsp;R Oskam,&nbsp;B A Van Der Zeijst,&nbsp;W Den Otter","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 1","pages":"55-66"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17742035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Induction of resistance to ectromelia virus infection by corynebacterium parvum in murine peritoneal macrophages. 小棒状杆菌诱导小鼠腹腔巨噬细胞抵抗嗜电性贫血病毒感染。
Journal of the Reticuloendothelial Society Pub Date : 1983-01-01
D A Cohen, H C Bubel
{"title":"Induction of resistance to ectromelia virus infection by corynebacterium parvum in murine peritoneal macrophages.","authors":"D A Cohen,&nbsp;H C Bubel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An in vitro model has been developed to study the replication of ectromelia virus in murine macrophages (M phi). Infection of mineral oil-elicited peritoneal M phi cultures with either the virulent (Moscow) or attenuated (Hampstead) strain of ectromelia virus led to productive infections. The kinetics of virus synthesis was similar to those seen following infection of murine fibroblasts. In contrast, peritoneal M phi s activated by intraperitoneal injection of Corynebacterium parvum vaccine were found to be totally refractory to infection by the attenuated strain and significantly more resistant to the virulent strain of ectromelia virus. Administration of C. parvum doses as small as 7 micrograms were sufficient to induce antiviral activity. M phi resistance became maximal at 5-9 days after C. parvum administration; however, M phi resistance was unstable during in vitro culture. Decay of antiviral activity was detected within the first 24 hr of culture and complete virus susceptibility returned after 5 days in culture. Peritoneal exudate cells (PEC) from C. parvum-immunized mice could induce resistance in susceptible M phi cultures during overnight cocultivation. In addition, cell-free culture supernatants from C. parvum-immune PEC could also induce resistance in susceptible M phi cultures, suggesting that a soluble factor, induced by C. parvum immunization and possessing interferon activity, may account for the intrinsic resistance to ectromelia virus by activated M phi s.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 1","pages":"35-46"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17362034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Amplification of the opsonic activity of fibronectin by a plasma factor lacking gelatin affinity. 缺乏明胶亲和力的血浆因子对纤维连接蛋白的拮抗活性的扩增。
Journal of the Reticuloendothelial Society Pub Date : 1983-01-01
F A Blumenstock, T M Saba, P M Cardarelli, E Cho
{"title":"Amplification of the opsonic activity of fibronectin by a plasma factor lacking gelatin affinity.","authors":"F A Blumenstock,&nbsp;T M Saba,&nbsp;P M Cardarelli,&nbsp;E Cho","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The opsonic activity of plasma fibronectin is important in modulating the reticuloendothelial system (RES) phagocytic removal of a variety of endogenous and exogenous particulate material from the vascular compartment. Purification of plasma-opsonic fibronectin by affinity chromatography with gelatin-Sepharose revealed that although in vitro hepatic Kupffer cell phagocytosis was absolutely dependent upon the presence of fibronectin, the purified fibronectin evaluated in concentrations similar to that found in plasma (350-450 micrograms/ml) supported phagocytosis at a level two- to threefold less than that observed in whole plasma. In contrast, the combination of purified fibronectin with small aliquots of opsonically inactive fibronectin-free plasma restored normal opsonic activity as assessed by liver slice bioassay and enhanced fibronectin-mediated attachment of gelatinized particulate to isolated Kupffer cells in vitro. Evidence is presented in this study that there exists in plasma a macromolecular species that amplifies the opsonic activity of fibronectin in a dose-related manner. This amplification or cofactor activity is nondialysable and has a molecular weight greater than 12,000. Inactivation of the amplification activity present in affinity-absorbed plasma can be achieved by heating the fibronectin-free plasma at 60 degrees C for 20 min, supporting the hypothesis that the cofactor is a protein. The amplification response is dose related, suggesting that the mechanism of its action is stoichiometric rather than catalytic. Evidence is presented that partial purification of the cofactor can be achieved by (NH4)2SO4 precipitation at 4 degrees C. Purification of this cofactor will provide an opportunity to evaluate its role in the altered opsonic states known to exist after trauma, burn, and sepsis.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 1","pages":"21-33"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17886167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
NCTC 1469 CB, a subline of the macrophage-like cell line NCTC 1469 with reduced phagocytic activity. NCTC 1469 CB，巨噬细胞样细胞系NCTC 1469的亚系，吞噬活性降低。
Journal of the Reticuloendothelial Society Pub Date : 1983-01-01
C D Van Basten, R A De Weger, H Van Loveren
{"title":"NCTC 1469 CB, a subline of the macrophage-like cell line NCTC 1469 with reduced phagocytic activity.","authors":"C D Van Basten,&nbsp;R A De Weger,&nbsp;H Van Loveren","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A variant subline, NCTC 1469 CB, of the macrophage-like cell line NCTC 1469 is described. NCTC 1469 CB cells are macrophage-like cells as the cells adhere, phagocytose carbon particles, have Fc and complement receptors and high levels of lysosomal enzymes. NCTC 1469 CB cells, however, do not phagocytose erythrocytes coated with IgG and complement, while the original NCTC 1469 cells phagocytose these coated erythrocytes in large amounts. The NCTC 1469 CB cells might be interesting for the study of mechanisms of Fc- and complement-mediated processes.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 1","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17886168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Suppressor alveolar macrophages in experimentally induced uremia. 抑制肺泡巨噬细胞在实验性尿毒症中的作用。
Journal of the Reticuloendothelial Society Pub Date : 1983-01-01
Y G Alevy, P Hutcheson, K R Mueller, R G Slavin
{"title":"Suppressor alveolar macrophages in experimentally induced uremia.","authors":"Y G Alevy,&nbsp;P Hutcheson,&nbsp;K R Mueller,&nbsp;R G Slavin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of uremic alveolar macrophages (AM phi) on the response of control nonadherent (NA) spleen cells to mitogens was examined. Alveolar macrophages purified from experimentally induced uremic rats were found to be significantly more suppressive than alveolar macrophages purified from control rats. When studying the characteristics of uremic AM phi, it was found that neither indomethacin nor anti-Ia antibody reverses their suppressive activity. In addition, uremic AM phi are shown to suppress via a suppressor factor released to the supernatant over an 18-hr incubation. The enhanced suppressive activity of uremic AM phi may have relevance to the severely suppressed cell-mediated immunity, the increased rate of infections, and the common presence of uremic pneumonitis in humans with chronic renal failure.</p>","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 1","pages":"11-20"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17628163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cultures of human natural killer cells (large granular lymphocytes) and T cells in the presence of interleukin-2-containing conditioned medium. 人自然杀伤细胞(大颗粒淋巴细胞)和T细胞在含白细胞介素-2的条件培养基中的培养。
Journal of the Reticuloendothelial Society Pub Date : 1983-01-01
T Timonen, J R Ortaldo, B M Vose, M Henkart, J Alvarez, R B Herberman
{"title":"Cultures of human natural killer cells (large granular lymphocytes) and T cells in the presence of interleukin-2-containing conditioned medium.","authors":"T Timonen,&nbsp;J R Ortaldo,&nbsp;B M Vose,&nbsp;M Henkart,&nbsp;J Alvarez,&nbsp;R B Herberman","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 1","pages":"67-80"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17657810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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