Journal of the Reticuloendothelial Society最新文献_第6页

Molecular weight, isoelectric point, and stability of a murine lymphokine that induces macrophage tumoricidal activity. 诱导巨噬细胞灭瘤活性的小鼠淋巴因子的分子量、等电点和稳定性。

Journal of the Reticuloendothelial Society Pub Date : 1983-05-01

S Yamamoto, E J Leonard, M Meltzer

引用次数: 0

Uptake by enterocytes and subsequent translocation to internal organs, eg, the thymus, of Percoll microspheres administered per os to suckling mice. 喂给哺乳小鼠的Percoll微球被肠细胞摄取并随后转运到内脏器官，如胸腺。

Journal of the Reticuloendothelial Society Pub Date : 1983-04-01

K Matsuno, T Schaffner, H A Gerber, C Ruchti, M W Hess, H Cottier

引用次数: 0

Alterations of the glycocalyx of Fc receptor-bearing cell lines during Fc receptor-ligand interactions. Fc受体-配体相互作用过程中携带Fc受体细胞系糖萼的改变。

Journal of the Reticuloendothelial Society Pub Date : 1983-04-01

K L Richards, S D Douglas

引用次数: 0

Asbestos exposure enhances the release of fibroblast growth factor by sheep alveolar macrophages. 石棉暴露增强羊肺泡巨噬细胞释放成纤维细胞生长因子。

Journal of the Reticuloendothelial Society Pub Date : 1983-04-01

I Lemaire, M Rola-Pleszczynski, R Bégin

{"title":"Asbestos exposure enhances the release of fibroblast growth factor by sheep alveolar macrophages.","authors":"I Lemaire, M Rola-Pleszczynski, R Bégin","doi":"","DOIUrl":"","url":null,"abstract":"Interaction between free airway cells (FAC) and lung fibroblasts was studied in a sheep model of asbestosis. Three groups of six sheep each received, respectively, by repeated intratracheal instillations, saline (control), 328 mg (low dose), and 2282 mg (high dose) of UICC chrysotile B asbestos. Sixteen months after the first instillation, FAC obtained by segmental bronchoalveolar lavage (BAL) of sheep in each group were incubated for various intervals, and the effect of their culture supernatants (FAC-SN) on human embryonic lung fibroblast proliferation was determined. FAC-SN from control animals stimulated thymidine (3H-TdR) incorporation by lung fibroblasts two- to threefold compared to untreated cultures. Maximal stimulation was observed at 48 hr and was correlated with a significant increase of the fibroblast population at 72 hr. FAC population from control sheep consisted primarily of macrophages (79%) and lymphocytes (15%), and separation of these two cell populations indicated that only macrophages produced the fibroblast-stimulating activity. Production occurred within 1 hr of incubation and was maximal between 2 and 4 hr. This activity was nondialyzable and stable at 56 degrees C for 30 min, but was destroyed at 80 degrees C and low pH. Moreover, FAC-SN from sheep exposed to asbestos stimulated 3H-TdR incorporation by fibroblasts five- to sixfold compared to two- to threefold for control FAC-SN. This activity may modulate fibrogenesis and may be involved in the eventual fibrogenic response to asbestos.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 4","pages":"275-85"},"PeriodicalIF":0.0,"publicationDate":"1983-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17284024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Particle binding, phagocytosis, and plastic substrate adherence characteristics of alveolar macrophages from rats acutely treated with chlorphentermine. 大鼠肺泡巨噬细胞的颗粒结合、吞噬和塑料底物粘附特性。

Journal of the Reticuloendothelial Society Pub Date : 1983-04-01

B E Lehnert, J Ferin

{"title":"Particle binding, phagocytosis, and plastic substrate adherence characteristics of alveolar macrophages from rats acutely treated with chlorphentermine.","authors":"B E Lehnert, J Ferin","doi":"","DOIUrl":"","url":null,"abstract":"The effects of acute treatment with the phospholipidosis-inducing agent chlorphentermine (CP) on alveolar macrophage (AM) function heretofore have not been investigated. We evaluated the effects of acute CP treatment on the functional status of AM by comparing Fc receptor (FcR) and \"nonspecific substance receptor\"-mediated particle binding and phagocytic activities, as well as the plastic substrate adherence characteristics of AM lavaged from control (CONT-AM) and CP-treated (CP-AM) rats. Acute CP treatment caused an impairment in AM phagocytosis and binding of sheep red blood cells opsonized with immunoglobulin G (IgG). Although CONT-AM and CP-AM avidities for tanned human red blood cells were indistinguishable, phagocytosis of these particles by CP-AM was diminished in a manner similar to that found for FcR-mediated phagocytosis. Analyses of the distributions of test particles in phagocytizing CONT-AM and CP-AM indicated that the phagocytic activity of a subset of CP-AM was compromised by drug treatment. Populations of CP-AM were also found to be less adherent in a plastic substrate adherence assay verified with a poorly adherent AM population. Unlike studies involving chronic CP-treatment, our studies indicate acute CP administration results in an impairment in AM phagocytic activity and a reduction in FcR-mediated particle binding.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 4","pages":"293-303"},"PeriodicalIF":0.0,"publicationDate":"1983-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17886056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of endotoxin in the expression of human monocyte cytotoxicity. 内毒素在人单核细胞毒性表达中的作用。

Journal of the Reticuloendothelial Society Pub Date : 1983-04-01

A Biondi, G Peri, R Lorenzet, D Fumarola, A Mantovani

{"title":"Role of endotoxin in the expression of human monocyte cytotoxicity.","authors":"A Biondi, G Peri, R Lorenzet, D Fumarola, A Mantovani","doi":"","DOIUrl":"","url":null,"abstract":"The role of bacterial endotoxin in the expression of human monocyte cytotoxicity was studied. Endotoxin contamination of all reagents and steps of the experimental procedure were assessed by the limulus amebocyte lysate (LAL) assay. Peripheral blood monocytes were separated by adherence, and cytotoxicity was measured as [3H]-thymidine release from prelabeled mKSA-TU5 murine target cells in a 48-hr assay. Human monocytes expressed appreciable levels of spontaneous cytotoxicity under LAL- conditions irrespective of the serum source employed (LAL- or LAL+ fetal bovine serum, FBS, or LAL- human cord serum, HCS). HCS gave the highest cytotoxicity levels and LAL- FBS the lowest. Polymyxin B (10 micrograms/ml), which inhibited endotoxin-induced gelation of LAL and activation of mononuclear cells for procoagulant activity, did not affect the expression of spontaneous monocyte cytotoxicity with all three sera used. Three preparations of endotoxin used in the present study (Escherichia coli, S. typhosa, S. minnesota) caused small increases in monocyte killing in micrograms per milliliter concentrations only in 5 of 19 experiments performed. Human lymphoblastoid interferon (LAL-) and phytohemagglutinin-elicited lymphokine supernatants (LAL-) enhanced the tumoricidal activity of human monocytes under LAL- conditions and were not affected by Polymyxin B. It is concluded that exposure to endotoxin is not a prerequisite for the expression of spontaneous cytotoxicity by human blood monocytes.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 4","pages":"315-27"},"PeriodicalIF":0.0,"publicationDate":"1983-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17252901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human neutrophil membrane topography: examination of distribution, movement, and regeneration of recognition sites using lectins as probes. 人中性粒细胞膜形貌:使用凝集素作为探针检测识别位点的分布、运动和再生。

Journal of the Reticuloendothelial Society Pub Date : 1983-04-01

D L Weinbaum, J A Sullivan, G L Mandell

{"title":"Human neutrophil membrane topography: examination of distribution, movement, and regeneration of recognition sites using lectins as probes.","authors":"D L Weinbaum, J A Sullivan, G L Mandell","doi":"","DOIUrl":"","url":null,"abstract":"We have studied the pattern of membrane binding site redistribution, movement, and reappearance in polarized and nonpolarized human neutrophils using fluorescein and rhodamine-labeled lectins as probes. In suspension, polymorphonuclear leukocytes (PMN) were spherical and displayed a random array of recognition sites for all of the probes. PMN polarized in suspension by 10(-6) M N-formyl-L-methionyl-L-phenylalanine (f-Met-Phe), and PMN attached to substrate accumulated the bound lectin recognition site complex at the uropod (for Con A; 92.0 +/- 0.2% of cells and 91.3 +/- 9.8% of cells, respectively). Glutaraldehyde fixation of neutrophils oriented in a chemotactic gradient prior to lectin addition revealed the innate unbound recognition site array. Unbound Con A recognition sites were clustered at the front of 74.7 +/- 0.8% of cells in a \"headlight\" pattern, but binding sites for other lectins were distributed randomly around the polarized cell. When bound Con A complexes are swept to the tail of the polarized living PMN, \"new\" unbound Con A binding sites appear at the front of the cell. Neither cycloheximide nor KCN nor colchicine interferred with new binding site appearance. Cytochalasin B and sodium iodacetate prevented PMN polarization and interfered with appearance of new receptors. This suggests that these fresh sites are uncovered, previously cryptic binding sites rather than newly synthesized structures. Lectin binding site topography and movement are related to the functional state of the PMN. Since both Con A and certain bacteria bind to mannose derivatives, we postulate that the \"headlight pattern\" and uncovering of fresh binding sites aid the PMN in engulfing organisms as the phagocyte moves forward.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 4","pages":"249-61"},"PeriodicalIF":0.0,"publicationDate":"1983-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17886171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redistribution of mouse spleen cell Fc receptors following treatment with mouse or human aggregated immunoglobulin G. 小鼠或人聚集免疫球蛋白G处理后小鼠脾细胞Fc受体的再分布。

Journal of the Reticuloendothelial Society Pub Date : 1983-04-01

G Teti, A Misefari, D Venza-Teti, M F La Via

{"title":"Redistribution of mouse spleen cell Fc receptors following treatment with mouse or human aggregated immunoglobulin G.","authors":"G Teti, A Misefari, D Venza-Teti, M F La Via","doi":"","DOIUrl":"","url":null,"abstract":"Mouse spleen cells treated with the Fc receptor ligands mouse IgG2b and human IgG, followed or not by a second antibody, exhibit different patterns of redistribution. In the work reported here we have examined the redistribution of Fc receptors (FcR) after binding of aggregated mouse IgG2b (Alg) or of Alg followed by anti-lg. We were particularly interested in learning whether binding of isologous Alg to FcR is followed by significant redistribution and shedding of Alg-FcR complexes. Mouse IgG2b alone will not induce capping even after 60 min at 37 degrees C. Human IgG induces some capping with minor shedding of complexes. Human IgG followed by anti-IgG readily induces capping by 15 min on 70% of the cells. This treatment also induces capping by 60 min at 20 degrees C on about 80% of the cells with a moderate degree of shedding of complexes. This is in agreement with the concept that the crosslinking required for FcR capping can be best induced with a second antibody. It is of interest, however, that heterologous IgG, unlike isologous, can induce a modest degree of capping and slight shedding even without the second antibody, suggesting that some crosslinking occurs with heterologous IgG.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 4","pages":"287-92"},"PeriodicalIF":0.0,"publicationDate":"1983-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17886055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prostaglandin biosynthesis by a human macrophage-like cell line, U937. 人巨噬细胞样细胞系U937的前列腺素生物合成。

Journal of the Reticuloendothelial Society Pub Date : 1983-03-01

M A Cobb, W Hsueh, L M Pachman, W T Barnes

引用次数: 0

Monocyte-mediated bone resorption involves release of nondialyzable substances in addition to prostaglandin. 单核细胞介导的骨吸收除前列腺素外还包括不可透析物质的释放。

Journal of the Reticuloendothelial Society Pub Date : 1983-03-01

B Rutherford, C L Trummel

引用次数: 0