Journal of the Reticuloendothelial Society最新文献_第5页

A morphological study of the role of phagocytes in the clearance of Staphylococcus aureus from the lung. 吞噬细胞在清除肺部金黄色葡萄球菌中的作用的形态学研究。

Journal of the Reticuloendothelial Society Pub Date : 1983-06-01

M F Lipscomb, J M Onofrio, E J Nash, A K Pierce, G B Toews

引用次数: 0

Endotoxin fails to induce procoagulant in C3H/HeJ exudate macrophages. 内毒素不能诱导C3H/HeJ渗出的巨噬细胞产生促凝剂。

Journal of the Reticuloendothelial Society Pub Date : 1983-06-01

J W Shands

引用次数: 0

Toxic oxygen species in monocyte-mediated antibody-dependent cytotoxicity. 单核细胞介导的抗体依赖性细胞毒性中的有毒氧。

Journal of the Reticuloendothelial Society Pub Date : 1983-06-01

S Seim, T Espevik

{"title":"Toxic oxygen species in monocyte-mediated antibody-dependent cytotoxicity.","authors":"S Seim, T Espevik","doi":"","DOIUrl":"","url":null,"abstract":"The role of toxic oxygen species in monocyte-mediated ADCC against the myelogenous cell line K-562 was investigated. Freshly isolated human monocytes caused 40% specific lysis of antibody-coated K-562 cells (Ab-K-562). Monocytes challenged with Ab-K-562 gave a small but definite luminol-dependent chemiluminescence response, indicating that a respiratory burst with generation of toxic oxygen species had been elicited. Generation of hydrogen peroxide in areas of close apposition between the monocyte and the Ab-K-562 plasma membranes was demonstrated by electron microscopy using precipitation of cerium ions as a cytochemical stain for hydrogen peroxide. Catalase inhibited the formation of cerium precipitates in the interaction zone between monocytes and Ab-K-562 cells. Despite evidence that toxic oxygen species were generated, the monocytes' cytolytic activity against Ab-K-562 was not inhibited by superoxide dismutase, catalase, or azide. Enzymatically generated fluxes of superoxide anion or hydrogen peroxide were not cytolytic to K-562 cells but did have a cytostatic effect. We conclude that toxic oxygen species are generated when human monocytes are challenged with Ab-K-562. However, these toxic oxygen species do not appear to be the major mediators of the monocytes' cytolytic activity in this experimental system.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 6","pages":"417-28"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17737420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison of three-dimensional growth characteristics of cultured primitive marrow stroma derived from genetically anemic and normal mice. 遗传性贫血小鼠与正常小鼠培养的原始骨髓基质三维生长特性的比较。

Journal of the Reticuloendothelial Society Pub Date : 1983-06-01

E Daniels

引用次数: 0

Interaction of latex-insolubilized endotoxins with murine macrophages: phagocytic responses of endotoxin-responsive (C3HeB/FeJ) and -unresponsive (C3H/HeJ) macrophages in vitro. 乳胶不溶性内毒素与小鼠巨噬细胞的相互作用:内毒素应答(C3HeB/FeJ)和不应答(C3H/HeJ)巨噬细胞的体外吞噬反应

Journal of the Reticuloendothelial Society Pub Date : 1983-05-01

S Lubinsky-Mink, P Munkenbeck, D C Morrison

{"title":"Interaction of latex-insolubilized endotoxins with murine macrophages: phagocytic responses of endotoxin-responsive (C3HeB/FeJ) and -unresponsive (C3H/HeJ) macrophages in vitro.","authors":"S Lubinsky-Mink, P Munkenbeck, D C Morrison","doi":"","DOIUrl":"","url":null,"abstract":"Insolubilized lipopolysaccharides (LPS) were prepared by covalently coupling LPS from polysaccharide-deficient S. minnesota R595 and polysaccharide-rich E. coli 055:B5 to carboxylated latex particles. The stability of these LPS-latex complexes was determined using several assays to detect soluble LPS following incubation at ambient and elevated temperatures. Resident and thioglycollate-elicited macrophages from both LPS-responder C3HeB/FeJ and LPS nonresponder C3H/HeJ mice were examined for their capacity to phagocytose the LPS particles following in vitro culture for various time periods. Uptake was demonstrated by an increase in the number of particles within the macrophages with increasing time of incubation. Rough polysaccharide-deficient LPS-latex particles were found to be more readily phagocytosed than control particles, whereas smooth polysaccharide-rich LPS particles were phagocytosed less readily than the controls. Qualitatively similar results were found in the relative rate of uptake of particles by the macrophages from the endotoxin-responsive and -unresponsive mouse strains used in this study.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 5","pages":"353-67"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17892916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of a gastrointestinal microflora on natural killer cell activity. 胃肠道菌群对自然杀伤细胞活性的影响。

Journal of the Reticuloendothelial Society Pub Date : 1983-05-01

K F Bartizal, C Salkowski, E Balish

{"title":"The influence of a gastrointestinal microflora on natural killer cell activity.","authors":"K F Bartizal, C Salkowski, E Balish","doi":"","DOIUrl":"","url":null,"abstract":"These studies demonstrate that the natural cytotoxicity of BALB/c mouse spleen cells for 51Cr-labeled YAC-1 cells can be significantly enhanced by microorganisms in the alimentary tract. Spleen cells from germfree BALB/c mice, euthymic, athymic, or non-nude background (+/+), had natural cell-mediated cytotoxicity for YAC-1 cells. Intestinal colonization with a few (flora-defined) or many (complex flora-conventionalized) microorganisms significantly enhanced natural cell-mediated cytotoxicity of athymic and euthymic mice over their germfree counterparts. Conversely, colonization of the alimentary tract of athymic and euthymic germfree mice with a pure culture of Candida albicans or colonization with Candida and a Bacillus sp. did not enhance natural cell-mediated cytotoxic activity over germfree levels. Spleen cells from germfree athymic mice were significantly more cytotoxic than spleen cells from germfree BALB/c mice that did not carry the nude gene (ie, +/+). In the germfree or gnotobiotic state, no difference in natural killer cell activity was evident between athymic (nu/nu) and heterozygous (+/nu) littermate mice; however, athymic (nu/nu) flora-defined or conventionalized mouse spleen cells were significantly more cytotoxic for YAC-1 cells than splenocytes from flora-defined or conventionalized heterozygous (+/nu) littermates. Spleen cells from BALB/c mice that were athymic (nu/nu) and colonized with a complex microbial flora (ie, conventionalized) had the highest percentage of cytotoxicity, at three different effector to target ratios, for YAC-1 cells. These studies indicate that the intestinal microflora can alter murine natural cell-mediated cytotoxicity.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 5","pages":"381-90"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17402661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endotoxin-induced interferon synthesis in macrophage cultures. 巨噬细胞内毒素诱导的干扰素合成。

Journal of the Reticuloendothelial Society Pub Date : 1983-05-01

E A Havell, G L Spitalny

{"title":"Endotoxin-induced interferon synthesis in macrophage cultures.","authors":"E A Havell, G L Spitalny","doi":"","DOIUrl":"","url":null,"abstract":"Pure cultures of murine bone marrow-derived macrophages produce interferon (IFN) after exposure to endotoxin. The levels of endotoxin-induced IFN were enhanced 5- to 20-fold by pretreating (priming) macrophages with murine interferons produced by either NDV-induced L cells, which consisted of a mixture of IFN alpha and IFN beta, or IFN gamma produced by spleen cells stimulated with phytohemagglutinin. Studies conducted on the kinetics of IFN release from endotoxin-induced macrophages demonstrated that peak synthesis occurred within 2-4 hr and was completed 6 hr after the start of treatment. The addition of actinomycin D to macrophages, up to 1 hr after exposure to endotoxin, completely inhibited release of interferon, thus indicating that gene transcription was required for interferon synthesis. The inclusion of cycloheximide in the medium of endotoxin or Poly(I) X Poly(C)-induced macrophages, although inhibiting 90% of protein synthesis, resulted in a superinducing effect, in that induced macrophages treated with cycloheximide produced higher levels of IFN than macrophages not treated with the inhibitor of protein synthesis. Antigenic characterization of macrophage IFNs revealed that endotoxin-induced IFN was neutralized to a higher degree than virus-induced IFNs derived from either macrophages or L cells.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 5","pages":"369-80"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17253566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clearance of lactate dehydrogenase by SJL/J mice infected with lactate dehydrogenase-elevating virus. 乳酸脱氢酶升高病毒感染SJL/J小鼠对乳酸脱氢酶的清除作用。

Journal of the Reticuloendothelial Society Pub Date : 1983-05-01

M A Brinton, P G Plagemann

{"title":"Clearance of lactate dehydrogenase by SJL/J mice infected with lactate dehydrogenase-elevating virus.","authors":"M A Brinton, P G Plagemann","doi":"","DOIUrl":"","url":null,"abstract":"The plasma level of lactate dehydrogenase (LDH) activity rises to about ten times the normal level by 4 days after infection of mice with lactate-dehydrogenase elevating virus (LDV). The levels of seven other enzymes are also increased, but to a lesser degree. SJL/J mice demonstrate a unique, genetically controlled 20-fold increase in the plasma level of LDH enzyme after LDV infection, as well as enhanced levels of the other plasma enzymes elevated by LDV infection. Comparison of virus infection in SJL/J and Swiss mice as well as in cultures of peritoneal exudate cells made from them indicated that the time course and extent of virus replication was similar for the two strains of mice. The rate of clearance of intravenously injected rabbit or mouse LDH was found to be impaired to a similar extent in LDV-infected SJL/J and Swiss mice. The effect of LDV infection on the levels of endogenous LDH released as a result of injection of carbon tetrachloride or tumor growth was also similar in the two strains of mice. These results suggest that LDV infection may specifically induce a greater influx of LDH into the plasma of SJL/J mice from an as-yet-unknown source than in other strains of mice.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 5","pages":"391-400"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17892918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular fate of phase I Coxiella burnetii in guniea pig peritoneal macrophages. 豚鼠腹腔巨噬细胞I期伯纳氏杆菌胞内命运。

Journal of the Reticuloendothelial Society Pub Date : 1983-05-01

J S Little, R A Kishimoto, P G Canonico

{"title":"Intracellular fate of phase I Coxiella burnetii in guniea pig peritoneal macrophages.","authors":"J S Little, R A Kishimoto, P G Canonico","doi":"","DOIUrl":"","url":null,"abstract":"Cultivated guinea pig peritoneal macrophages were infected with radio-labeled phase I Coxiella burnetii in order to assess the intracellular distribution of ingested rickettsiae. Localization of organisms was determined by fractionation of macrophage homogenates by equilibrium density centrifugation on sucrose gradients. Macrophages isolated from either nonimmune or immune guinea pigs and infected with C burnetii opsonized with immune serum yielded equilibrium density distribution for rickettsiae similar to lysosomal enzymes, suggesting sequestration within macrophage lysosomes. To confirm these observations nonimmune or immune guinea pigs were injected with Triton WR-1339 prior to macrophage harvest to decrease the density of macrophage lysosomes. Triton-laden macrophages infected with opsonized rickettsiae resulted in equilibrium density distribution for lysosomal enzymes and organisms in less dense regions of the gradient. In contrast, when either nonimmune or immune macrophages were infected in the presence of normal guinea pig serum, the distribution of labeled rickettsiae in the gradient did not correspond with lysosomes. We conclude that in the absence of immune serum, ingested C burnetii are not sequestered within macrophage lysosomes. Phagolysomal fusion and subsequent degradation of rickettsiae within the lysosomes of the macrophages appear to occur only when C burnetii are opsonized with immune serum.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 5","pages":"331-41"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17892915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of particulate beta-1,3 glucan on human, rat, and guinea pig complement activity. 颗粒β -1,3葡聚糖对人、大鼠和豚鼠补体活性的影响。

Journal of the Reticuloendothelial Society Pub Date : 1983-05-01

M M Glovsky, L Cortes-Haendchen, L Ghekiere, A Alenty, D L Williams, R Di Luzio

{"title":"Effects of particulate beta-1,3 glucan on human, rat, and guinea pig complement activity.","authors":"M M Glovsky, L Cortes-Haendchen, L Ghekiere, A Alenty, D L Williams, R Di Luzio","doi":"","DOIUrl":"","url":null,"abstract":"Particulate glucan, a beta-1,3-linked polyglucose derived from Saccharomyces cerevisiae, has been demonstrated to have a wide range of immunopotentiating effects. Glucan administration is associated with the modification of a variety of experimentally induced infectious disease states as well as the inhibition of growth of implantable and spontaneous tumors. The present study was designed to evaluate the effect of glucan upon activation of the complement system in rats and guinea pigs. Additional studies were performed to determine the in vitro activating effect of glucan and zymosan on complement activity of human serum. Glucan activated both the classical pathway of normal human sera and the alternate pathways in C2hu-deficient sera in vitro releasing anaphylatoxins such as C3a. The intravenous injection of glucan activated the alternate pathway of guinea pig plasma. The influence of glucan on complement depletion induced by cobra venom factor (CVF) was also ascertained. Complement activation by glucan may contribute, in part, to the enhanced resistance of the host against tumor growth as well as infectious episodes.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 5","pages":"401-13"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17657622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0