Journal of submicroscopic cytology and pathology最新文献_第10页

Scanning electron microscopic observations of the development of the chicken caecum. 鸡盲肠发育的扫描电镜观察。

Journal of submicroscopic cytology and pathology Pub Date : 2003-10-01

O Sabatakou, E Paraskevakou, S Tseleni-Balafouta, A Athanasiadis, C Fasseas

引用次数: 0

Cholesterol oxidase and the hydroxymethylglutaryl coenzyme A reductase inhibitor mevinolin perturb endocytic trafficking in cultured vascular smooth muscle cells. 胆固醇氧化酶和羟甲基戊二酰辅酶A还原酶抑制剂甲维苷干扰培养血管平滑肌细胞的内吞运输。

Journal of submicroscopic cytology and pathology Pub Date : 2003-10-01

J Thyberg

{"title":"Cholesterol oxidase and the hydroxymethylglutaryl coenzyme A reductase inhibitor mevinolin perturb endocytic trafficking in cultured vascular smooth muscle cells.","authors":"J Thyberg","doi":"","DOIUrl":"","url":null,"abstract":"Cholesterol is a component of cellular membranes and especially abundant in caveolae (50-80 nm flask-shaped invaginations of the plasma membrane). Caveolae are highly numerous in vascular endothelial and smooth muscle cells and have been implicated in a variety of functions, including signal transduction, lipid transport and uptake of macromolecules. Here, the effects of cholesterol oxidase (an enzyme that oxidizes cholesterol in caveolae of living cells) and mevinolin (an inhibitor of cholesterol synthesis) on fine structure and internalization of exogenous markers were studied in rat aortic smooth muscle cells grown on a substrate of fibronectin in serum-free primary cultures. Cholesterol oxidase caused a growth in size of the endocytic compartment with accumulation of enlarged endosomes and lysosomes containing tracer molecules. In parallel, the number of caveolae was reduced by about one fifth. Moreover, the morphology of the Golgi complex was altered with swollen cisternae surrounded by empty-looking vacuoles. Mevinolin suppressed transition of the cells from a differentiated or contractile to a dedifferentiated or synthetic phenotype. In addition, contractile cells were found to ingest horseradish peroxidase (HRP) not only into endosomes and lysosomes but also into Golgi cisternae, especially on the convex/cis side of the stacks, and the endoplasmic reticulum. A similar pathway was noted in contractile cells exposed to cholera toxin B subunit (CTB)-HRP conjugates, a ligand that binds to ganglioside GM1 and at least in part is ingested via caveolae. Mevinolin did not prevent the transport of CTB-HRP to the Golgi complex, but the conjugates were in this case concentrated to the concave/trans side of the cisternal stacks. However, no clear effect on the number of caveolae was noted. The observations indicate an important role of cholesterol and caveolae in the control of endocytic traffic in smooth muscle cells. This function appears most significant when the cells are in a differentiated state.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"35 4","pages":"457-68"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24512963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural, cytochemical, immunocytochemical and ultrastructural characterization of blood granulocytes of the roadside hawk Buteo magnirostris (Gmelin, 1788) (Avian, Falconiform). 路边鹰Buteo magnirostris (Gmelin, 1788) (Avian, Falconiform)血液粒细胞的结构、细胞化学、免疫细胞化学和超微结构表征

Journal of submicroscopic cytology and pathology Pub Date : 2003-10-01

A A Santos, A Marques Joppert Da Silva, M R Regis Silva, H R Cômodo Segreto, M Imoto Egami

{"title":"Structural, cytochemical, immunocytochemical and ultrastructural characterization of blood granulocytes of the roadside hawk Buteo magnirostris (Gmelin, 1788) (Avian, Falconiform).","authors":"A A Santos, A Marques Joppert Da Silva, M R Regis Silva, H R Cômodo Segreto, M Imoto Egami","doi":"","DOIUrl":"","url":null,"abstract":"In the peripheral blood of the roadside hawk, Buteo magnirostris, the following types of granulocytic leucocytes were identified: heterophil, eosinophil and basophil. The heterophils presented acidophilic and spindle shaped granules, the eosinophils possess spherical eosinophilic granules and the basophils showed spherical and basophilic granules. The heterophils and eosinophils presented positive cytochemical reaction to glycogen and basic polyaminoacid, while the eosinophils presented sudanophilic granules, which were also positive for myeloperoxidase. The heterophils, alone, presented positivity for acid phosphatase in some granules and immunoreactivity to TGF-beta1 was observed only in the cytoplasm of the eosinophils. Electron microscopy demonstrated the heterophil granules as predominantly spindle shaped, being strongly electron-dense, while the eosinophils had numerous uniformly electron-dense spherical granules and the basophils presented three different types of granules identified according to their electron-density and the aspect of their matrix.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"35 4","pages":"351-7"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24513598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The wart-like chorion of Edessa bifida (Hemiptera: Pentatomidae). 裂腹蝶的疣状绒毛膜(半翅目:裂腹蝶科)。

Journal of submicroscopic cytology and pathology Pub Date : 2003-10-01

K W Wolf, W Reid

引用次数: 0

Ultrastructure of the external gill epithelium of the axolotl, Ambystoma mexicanum with reference to ionic transport. 墨西蝾螈外鳃上皮超微结构与离子传输。

Journal of submicroscopic cytology and pathology Pub Date : 2003-10-01

M S Jarial, J H Wilkins

{"title":"Ultrastructure of the external gill epithelium of the axolotl, Ambystoma mexicanum with reference to ionic transport.","authors":"M S Jarial, J H Wilkins","doi":"","DOIUrl":"","url":null,"abstract":"The ultrastructure of the external gill epithelium of the axolotl, Ambystoma mexicanum, has been examined using conventional transmission electron microscopy to elucidate its role in ionic transport. Four cell types are identified in the gill filament and primary gill bar epithelium. These are granular, ciliated, Leydig and basal cells. A fifth cell type, the flat mitochondria-rich cell is only found in the gill bar epithelium. The predominant granular cells display microvilli at their surface and their cytoplasm contains abundant mitochondria, rough endoplasmic reticulum, Golgi complexes, vesicles and PAS+ secretory granules that are extruded at the surface, which along with secretions from the Leydig cells form a mucous coat. The granular cells are joined apically by junctional complexes consisting of zonulae occludens, zonulae adherens and desmosomes. The lateral membranes of granular cells enclose large intercellular spaces that are closed at the apical ends but remain open at the basal ends adjoining capillaries. In AgNO3-treated axolotl, the gills become darkly stained, the silver grains penetrate apical membranes and appear in the cytoplasm, accumulating near the lateral membranes and also enter the intercellular spaces. These findings are consistent with the dual role of the gill epithelium in mucus production and active ionic transport.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"35 4","pages":"445-55"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24512962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eyes covered by mitochondrial lenses in Petaliella spiracauda and Ptychopera purasjokii (Plathelminthes, Rhabdocoela, Trigonostominae). Ultrastructural features and phylogenetic implications. 螺旋体Petaliella spiracauda和Ptychopera purasjokii的眼睛被线粒体透镜覆盖(platelmintes, Rhabdocoela, Trigonostominae)。超微结构特征及其系统发育意义。

Journal of submicroscopic cytology and pathology Pub Date : 2003-10-01

B Sopott-Ehlers, U Ehlers

{"title":"Eyes covered by mitochondrial lenses in Petaliella spiracauda and Ptychopera purasjokii (Plathelminthes, Rhabdocoela, Trigonostominae). Ultrastructural features and phylogenetic implications.","authors":"B Sopott-Ehlers, U Ehlers","doi":"","DOIUrl":"","url":null,"abstract":"The submicroscopic anatomy of the eyes in Petaliella spiracauda and Ptychopera purasjokii is described. These eyes correspond in general to the basic pattern of rhabdomeric pigment-cup ocelli. This, however, does not apply to modifications of the cup cell such as the differentiation of mitochondrial lenses. Corresponding with two sensory cells two extensions of the cup cell capping the eye aperture are crowded with small unmodified mitochondria in the eyes of P. spiracauda. The eyes of P. purasjokii have three sensory cells and the lenticular element is formed by a trifoil-shaped differentiation of three giant mitochondrial derivatives. These derivatives show peripheral appendages of various configurations, all of which resemble the profiles of small mitochondria. The implication of the existence of such appendages is that the lenses in P. purasjokii are derived from many fused mitochondria, rather than from a single enlarged one. It is concluded that the unmodified or modified mitochondrial differentiations in proliferations of the pigment cell capping the opening of the eye cup serve to focalize incoming light. The evolution of mitochondrial lenses in Plathelminthes is considered.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"35 4","pages":"415-21"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24512958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular response to an intravascular catheter. 细胞对血管内导管的反应。

Journal of submicroscopic cytology and pathology Pub Date : 2003-07-01

L T Chen, C P Phelps, M W Bryant, M E Chen

引用次数: 0

Protein phosphorylation during the process of interaction of Toxoplasma gondii with host cells. 刚地弓形虫与宿主细胞相互作用过程中的蛋白磷酸化。

Journal of submicroscopic cytology and pathology Pub Date : 2003-07-01

S Ferreira, T M U De Carvalho, W De Souza

{"title":"Protein phosphorylation during the process of interaction of Toxoplasma gondii with host cells.","authors":"S Ferreira, T M U De Carvalho, W De Souza","doi":"","DOIUrl":"","url":null,"abstract":"Previous studies have shown that tachyzoites of Toxoplasma gondii were able to penetrate into macrophages using two mechanisms: phagocytosis and active penetration. We show here that previous incubation of the macrophages or the parasites with staurosporine, a wide range inhibitor of protein kinases, tyrfostin and genistein, specific inhibitors of tyrosine kinases, significantly interfered with the process of parasite-macrophage interaction. Staurosporine treatment induced the formation of many filopodium-like surface projections of the macrophages and markedly increased the attachment of the tachyzoites to the cell surface. Genistein inhibited about 50% penetration of T. gondii into macrophages. Previous incubation of tachyzoites with genistein also significantly inhibited their attachment to and penetration into the macrophages. Confocal laser scanning microscopy was used to locate phosphoproteins in macrophages interacting with tachyzoites. Antiphosphotyrosine antibodies labeled the surface of macrophages, but not L929 cells, incubated in presence of T. gondii, even those cells did not show associated parasites. Anti phosphotyrosine, phosphothreonine and phosphoserine antibodies labeled the region surrounding the parasitophorous vacuoles. These observations suggest that protein phosphorylation is a key event in the process of T. gondii-host cell interaction.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"35 3","pages":"245-52"},"PeriodicalIF":0.0,"publicationDate":"2003-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24137294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural alterations in cortex of adrenal gland caused by the toxic effect of bee (Apis mellifera) venom. 蜜蜂毒液毒性作用对肾上腺皮质超微结构的影响。

Journal of submicroscopic cytology and pathology Pub Date : 2003-07-01

A Rodríguez-Acosta, J Vega, H J Finol, M Pulido-Mendez

{"title":"Ultrastructural alterations in cortex of adrenal gland caused by the toxic effect of bee (Apis mellifera) venom.","authors":"A Rodríguez-Acosta, J Vega, H J Finol, M Pulido-Mendez","doi":"","DOIUrl":"","url":null,"abstract":"Bee accidents incidence is underestimated because many people do not consult to the physicians. Here it is described for the first time the severe mice adrenal gland damage induced by Apis mellifera venom. Biopsy specimens were obtained from mice adrenal gland and after sample preparation observed in Hitachi H-7100 electron microscope. In this work the ultrastructural analysis showed, 6 h after injection, a non homogeneous smooth endothelial reticulum, and in some places loss of plasma membrane. The fenestrae spaces were bigger and detritus in the capillary lumen were observed. Erythrocytes were seen in a cortical cell. After 48 h of venom injection, expanded fenestrae were observed. Capillary basal membrane was interrupted. Myelin-like figures and autophagic vacuoles were noticed. Swollen smooth endoplasmic reticulum elements and endothelial unfolding to the light were seen. Moreover, swollen Golgi and mitochondria were observed, in some places forming myelinic-like figures. At 144 h after venom injection, widened spaces were noticed in capillary fenestrae. Cellular section showed swollen and lost smooth endoplasmic reticulum elements. Smooth endoplasmic reticulum tubules disappearance suggested non steroidogenesis. In conclusion, we suggest that some of the bee envenoming human clinical manifestations, as is observed in mice, are determined by suprarenal gland damage produced by toxins present in this venom.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"35 3","pages":"309-14"},"PeriodicalIF":0.0,"publicationDate":"2003-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24137254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology of the vas deferens in an ethanol-drinking strain of rats (UChA and UChB). 大鼠嗜乙醇品系(UChA和UChB)输精管形态。

Journal of submicroscopic cytology and pathology Pub Date : 2003-07-01

J H Rissato, M V Ietsugu, C C D Almeida, P F F Pinheiro, T M Segatelli, M Martinez, C R Padovani, W M Júnior, V H A C Quitete, F E Martinez

引用次数: 0