墨西蝾螈外鳃上皮超微结构与离子传输。

M S Jarial, J H Wilkins
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摘要

用常规透射电镜研究了美西螈(Ambystoma mexicanum)外鳃上皮的超微结构,以阐明其在离子传输中的作用。在鳃丝上皮和原代鳃杆上皮中鉴定出四种细胞类型。它们是颗粒细胞、纤毛细胞、间质细胞和基底细胞。第五种细胞类型是扁平的富含线粒体的细胞,只存在于鳃杆上皮中。主要的颗粒细胞表面显示微绒毛,细胞质含有丰富的线粒体、粗糙的内质网、高尔基复合物、囊泡和表面挤出的PAS+分泌颗粒,这些颗粒与间质细胞的分泌物形成一层粘膜。颗粒细胞由闭塞小带、粘附小带和桥粒组成的连接复合体在顶部连接。颗粒细胞的侧膜包围着巨大的细胞间隙,这些间隙在顶端是封闭的,但在靠近毛细血管的基端保持开放。经agno3处理的蝾螈鳃染成黑色,银粒穿透顶膜出现在细胞质中,并在侧膜附近积聚并进入细胞间隙。这些发现与鳃上皮在粘液产生和活性离子运输中的双重作用是一致的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultrastructure of the external gill epithelium of the axolotl, Ambystoma mexicanum with reference to ionic transport.

The ultrastructure of the external gill epithelium of the axolotl, Ambystoma mexicanum, has been examined using conventional transmission electron microscopy to elucidate its role in ionic transport. Four cell types are identified in the gill filament and primary gill bar epithelium. These are granular, ciliated, Leydig and basal cells. A fifth cell type, the flat mitochondria-rich cell is only found in the gill bar epithelium. The predominant granular cells display microvilli at their surface and their cytoplasm contains abundant mitochondria, rough endoplasmic reticulum, Golgi complexes, vesicles and PAS+ secretory granules that are extruded at the surface, which along with secretions from the Leydig cells form a mucous coat. The granular cells are joined apically by junctional complexes consisting of zonulae occludens, zonulae adherens and desmosomes. The lateral membranes of granular cells enclose large intercellular spaces that are closed at the apical ends but remain open at the basal ends adjoining capillaries. In AgNO3-treated axolotl, the gills become darkly stained, the silver grains penetrate apical membranes and appear in the cytoplasm, accumulating near the lateral membranes and also enter the intercellular spaces. These findings are consistent with the dual role of the gill epithelium in mucus production and active ionic transport.

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