路边鹰Buteo magnirostris (Gmelin, 1788) (Avian, Falconiform)血液粒细胞的结构、细胞化学、免疫细胞化学和超微结构表征

A A Santos, A Marques Joppert Da Silva, M R Regis Silva, H R Cômodo Segreto, M Imoto Egami
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引用次数: 0

摘要

在路边鹰(Buteo magnirostris)的外周血中,发现了以下类型的粒细胞白细胞:嗜中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞。嗜酸性粒细胞呈嗜酸性和纺锤形颗粒,嗜酸性粒细胞呈球形颗粒,嗜碱性粒细胞呈球形和嗜碱性颗粒。嗜中性粒细胞和嗜酸性粒细胞对糖原和碱性多氨基酸呈阳性细胞化学反应,嗜酸性粒细胞呈嗜苏丹颗粒,对髓过氧化物酶也呈阳性反应。嗜酸性粒细胞在一些颗粒中呈酸性磷酸酶阳性,对tgf - β 1的免疫反应仅在嗜酸性粒细胞的细胞质中观察到。电镜观察显示,嗜异粒细胞颗粒主要呈纺锤形,具有很强的电子密度,而嗜酸性细胞有许多均匀的电子密度球形颗粒,嗜碱性细胞根据其电子密度和基质的外观表现出三种不同类型的颗粒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structural, cytochemical, immunocytochemical and ultrastructural characterization of blood granulocytes of the roadside hawk Buteo magnirostris (Gmelin, 1788) (Avian, Falconiform).

In the peripheral blood of the roadside hawk, Buteo magnirostris, the following types of granulocytic leucocytes were identified: heterophil, eosinophil and basophil. The heterophils presented acidophilic and spindle shaped granules, the eosinophils possess spherical eosinophilic granules and the basophils showed spherical and basophilic granules. The heterophils and eosinophils presented positive cytochemical reaction to glycogen and basic polyaminoacid, while the eosinophils presented sudanophilic granules, which were also positive for myeloperoxidase. The heterophils, alone, presented positivity for acid phosphatase in some granules and immunoreactivity to TGF-beta1 was observed only in the cytoplasm of the eosinophils. Electron microscopy demonstrated the heterophil granules as predominantly spindle shaped, being strongly electron-dense, while the eosinophils had numerous uniformly electron-dense spherical granules and the basophils presented three different types of granules identified according to their electron-density and the aspect of their matrix.

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