Journal of Soil Science and Plant Nutrition最新文献

{"title":"Single-Strain Inoculation of Bacillus subtilis and Rhizobium phaseoli Affects Nitrogen Acquisition of an Improved Mungbean Cultivar","authors":"Lisa Pataczek, Juan Carlos Barroso Armas, Theresa Petsch, Thomas Hilger, Maqshoof Ahmad, Roland Schafleitner, Zahir Ahmad Zahir, Georg Cadisch","doi":"10.1007/s42729-024-02001-7","DOIUrl":"https://doi.org/10.1007/s42729-024-02001-7","url":null,"abstract":"<p>Plant growth-promoting rhizobacteria (PGPR) increase plant root growth, potentially improving soil nitrogen (N) uptake, and productivity. Legumes, for instance mungbean, could also benefit from a rise in potential infection sites for nodulation, thereby increasing rates of biological N₂ fixation (BNF). Consequently, the objectives of this study were (i) to assess whether PGPR had an effect on mungbean root biomass and if that was linked to N accumulation and productivity; (ii) to identify whether multi-strain inoculation showed greater efficacy in increasing N accumulation and overall productivity than single-strain inoculation; (iii) to test whether N acquisition was based on BNF rather than on soil N uptake. Field trials were conducted in two seasons at the University of Agriculture, Faisalabad with mungbean cultivar NM11 and multi-strain inoculation consisting of <i>Rhizobium phaseoli</i>, <i>Bacillus subtilis</i>, and <i>Pseudomonas fluorescens</i>. The strains were tested additionally in the second season as single-strain inoculation. Multi-strain and inoculation with <i>P. fluorescens</i> alone had no effect on root biomass, total plant-N, BNF or soil N uptake. Inoculation with <i>B. subtilis</i>, however, resulted in significantly increased root dry matter (+ 211 kg ha^− 1), total dry matter (+ 1.7 t ha^− 1), and total plant-N (+ 36 kg ha^− 1). Only inoculation with <i>R. phaseoli</i> enhanced BNF (+ 24%). Yield was not affected by any inoculation. The results suggested that total plant-N was based on soil N uptake rather than on BNF and demonstrated that only single strains affected total N accumulation, pointing to antagonistic mechanisms of the strains in a mixed inoculum.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobiochar-Coating Regulates N and P Release from DAP Fertilizer in Soil and Improves Maize Crop Productivity","authors":"Ghulam Abbas Shah, Mughees Mustafa, Hani Z. Asfour, Khubra Shoukat, Ahsan Yasin, Nadeem Ali, Muhammad Bilal Khan Niazi, Gabrijel Ondrasek, Muhammad Imtiaz Rashid","doi":"10.1007/s42729-024-02004-4","DOIUrl":"https://doi.org/10.1007/s42729-024-02004-4","url":null,"abstract":"<p>The crop phosphorus (P) utilization efficiency of commercial fertilizers is only 10–15%, leaving much P fixed in the soil. Coating fertilizer can lessen this problem, but most of the current available options are potentially toxic and expensive. This study-investigated nanobiochar as a coating material for engineering “smart” di-ammonium phosphate (DAP) fertilizer that controls P and nitrogen (N) release in soil, ultimately enhancing nutrient utilization by maize. Biochar was produced from farmyard manure and ball-milled to obtain nanobiochar. Different nanobiochar concentrations (2.5%, 5%, and 10% w/w) were used to coat the DAP granules in a fluidized-bed coater. The release of N and P was studied after immersing both coated and uncoated DAP fertilizers in water. In a pot experiment, five treatments, i.e.i) control (C), ii) uncoated DAP (UF), iii) 2.5% nanobiochar-coated DAP (CUNB1), iv) 5% nanobiochar-coated DAP (CUNB2), and v) 10% nanobiochar-coated DAP (CUNB3) were introduced, after which maize was sown. The presence of a uniform nanobiochar coating on DAP was confirmed by the discrete carbon peaks observed through X-ray diffraction and FTIR spectroscopic analyses. In a laboratory study, the slowest release of N and P was observed for CUNB3. Remarkably, the application of CUNB1 substantially increased the microbial biomass carbon and N by 104% and 147%, respectively, while enhancing the plant-available P, N, and potassium (K) by 40%, 70%, and 46%, respectively, compared with those of C. This treatment increased maize shoot dry matter yield by 88%, accompanied by marked increases of 229%, 205%, and 67% in maize P, N, and K uptakes compared to C, respectively. However, other coating treatments failed to increase these parameters compared with those of UF, confirming that these coatings had the slowest nutrient availability for short-duration crops. The 2.5% nanobiochar concentration can be recommended for coating DAP fertilizer to reduce problems of P fixation and enhance P availability, crop growth and nutrients uptake, hence contributing to sustainable fertilizer management practices in agroecosystem.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil Nematodes in Agroecosystems: Linking Cropping System’s Rhizosphere Ecology to Nematode Structure and Function","authors":"Abile Teshita, Waqif Khan, Abd Ullah, Babar Iqbal, Naveed Ahmad","doi":"10.1007/s42729-024-01982-9","DOIUrl":"https://doi.org/10.1007/s42729-024-01982-9","url":null,"abstract":"<p>Land use change and intensification though they contributed to increases in food production, have remained one of the main threats to soil biodiversity due to their negative impacts on the health and fertility of the soil. Nematodes have been used as a tool for assessing the structure and functions of soils in agroecosystems because indices of nematode community can reflect current changes and functions over time of the ecological processes in the soil. Although nematodes are largely considered important drivers in the decomposition of organic matter and nutrient cycling, their community structure and functional responses to land use change and intensification, and agricultural practices remain poorly understood. Therefore, this review aims to evaluate the response of soil nematodes to land use change and intensification, as well as the potential influence of management practices on their community structure and population dynamics. Besides, due to the fact that nematodes are soil inhabitants, their activities are largely controlled by the physical and biological conditions of the soil. A variation in the soil micro-ecological environment may affect their community structure and functional responses. Furthermore, we investigate the impact of agricultural intensification, such as monocropping, greater use of chemical fertilizers, and the application of pesticides on nematode populations. We also evaluate how sustainable agricultural techniques like organic farming, crop rotation, and decreased tillage affect the health of nematode populations. This study will give a thorough knowledge of how these factors interact to affect soil health and ecosystem function. Further insights about how root interactions in multi-species systems affect the rhizosphere ecology and influence the nematode community will be discussed.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apple-Based Farming Systems Improve Production and Rhizosphere Microbiome in The North-West Himalayan Region's Dry Temperate Environment","authors":"Himanshu Mehta, Pramod Kumar, N. C. Sharma, Anjali Chauhan, Arun Negi, D. P. Sharma","doi":"10.1007/s42729-024-02006-2","DOIUrl":"https://doi.org/10.1007/s42729-024-02006-2","url":null,"abstract":"<p>This research monitored the effect of integrated fruit-based farming system on production and rhizosphere microbiome of apple under dry temperate climate. The study also aims to understand the relationships among soil properties, production parameters and fruit yield of apple trees. Six cropping systems including, apple + pea, apple + kidney beans, apple + barley, apple + maize, apple + buckwheat, apple + garlic and apple monoculture were studied. The cropping systems treatments were cultivated with integrated farming approach, where measurements of the key indicators of productivity and nutrient cycling. Three blocks of district Kinnaur viz<i>.,</i> Nichar, Kalpa and Pooh were selected. A representative sample size of five sub-locations with four orchards in each sub-location and six apple-based cropping systems (CS) was collected from each block. The current study also examined the significance of microbial communities on nutrient dynamics and biological cycling on apple in legumes, pulses and millets-based cropping systems. Apple + pea increased plant height, tree girth, canopy diameter, shoot growth, and apple leaf area of trees. Maximum generative traits of trees were recorded for apple + pea and apple + kidney bean cropping systems. Fruit yield in apple + pea was determined higher than apple + buckwheat. Soil pH changed towards neutral. When compared to monoculture, apple + pea cropping system showed an increase in post-harvest soil chemical indicators. Microbial biomass in terms of bacteria, actinobacteria, soil fungi, AM fungi, <i>Azotobacter</i> and phosphorus solubilizing bacteria were also improved. Path analysis revealed a positive direct influence of soil chemical and microbial properties on yield. PCA determined that the first principal component caused maximum cumulative variance of 97.19 per cent. Soil organic carbon, microbial biomass carbon, rhizosphere microbial population and nutrient availability were improved as a consequence of intercropped residues left over after harvest. In this study, apple-based cropping systems significantly improved nutrient dynamics, rhizosphere and microbial biomass due to crop residual management by intercropped companion crops left over in soil. Adoption of apple with pea intercrop has shown significant improvements in yield and soil fertility compared to conventional methods. The study thus concluded that transitioning to cropping systems has positive effects on apple cultivation which can be a viable alternative to conventional farming.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar Amendments to Improve Soil Phosphorus Fertility and Retention in Canadian Prairie Soils","authors":"Anèl Dannhauser, Jeff J. Schoenau, Ryan D. Hangs, Biswa R. Patra, Ajay K. Dalai","doi":"10.1007/s42729-024-01998-1","DOIUrl":"https://doi.org/10.1007/s42729-024-01998-1","url":null,"abstract":"<p>The utilization of biochar, a carbonaceous substance derived from pyrolysis, has been extensively investigated in various agricultural settings. However, applying biochar to Canadian prairie soils without additional fertilizer treatments generates minimal benefits for crop productivity. This study investigated the effects of biochar amendments, with and without addition of Triple Superphosphate (TSP) fertilizer, on phosphorus (P) availability and retention in Canadian prairie crops and soils. Specifically, the study assessed crop yield, P uptake and recovery by canola plants, soil P retention, infiltration rates and P losses in simulated snowmelt runoff. Controlled environment and field studies were conducted with biochar and TSP fertilizer on nutrient deficient soils in the Saskatchewan Brown and Black soil zones. Under both growth chamber and field conditions, biochar derived from canola hull, manure, and willow feedstocks were shown to contribute some available P for plant uptake, with observed recovery of biochar P by canola up to ca. 50% of that found for TSP fertilizer. Among these biochar feedstocks canola meal biochar was least effective in supplying plant available P in the year of application. Willow biochar applied alone, or co-applied with TSP, may be an effective strategy for reducing P losses in snowmelt runoff compared with TSP alone and willow biochar also contributed to increased water infiltration. Biochars can potentially benefit canola production by enhancing P nutrition and recovery. Moreover, a balance may be obtained between biochar supplying P during the growing season, while reducing P losses in the spring snowmelt runoff.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Nitrogen and Water on Barley Grain Yield and Malting Quality","authors":"Davide Cammarano, Jonathan Holland, Alberto Gianinetti, Marina Baronchelli, Domenico Ronga","doi":"10.1007/s42729-024-01999-0","DOIUrl":"https://doi.org/10.1007/s42729-024-01999-0","url":null,"abstract":"<p>Barley is among the most important crops in northern latitudes especially for malting and distilling. Inter-annual weather variability in terms of rainfall and temperature patterns can impact crop uptake of soil water and nitrogen, which influences the crop growth and development. The present study shows the effects of nitrogen and water applied on: (i) specific grain quality traits necessary for distilling; (ii) plant biomass, nitrogen, and yield; and (iii) farmer’s marginal net return. The experiment was conducted during the growing seasons of 2018 and 2019 at the James Hutton Institute (UK) with two nitrogen fertilizers and two irrigation levels. During the growing season soil mineral nitrogen and soil water content and plant biomass and nitrogen were measured. At harvest yield, yield component, and grain quality traits were determined.2018 was a very dry growing season, as opposed to the wetter 2019 respect to the long-term growing season rainfall (1974–2017). Grain yield in 2018 was higher for the irrigated treatment, but in 2019 the irrigation, due to high rainfall, had lower yield. Environmental conditions impacted grain quality, and the patterns of soil water and mineral N affecting the final quality traits. Despite variable weather conditions the grain quality requirements from the industry of either beer or whisky are met.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vermicompost Addition Improved Soil Aggregate Stability, Enzyme Activity, and Soil Available Nutrients","authors":"Zhuo Yang, Yuxin Luo, Huan Chen, Ying Zhang, Shiyun Wu, Ming Yang, Jingjing Jia, Chunliang Zhou, Yumei Zhou","doi":"10.1007/s42729-024-02002-6","DOIUrl":"https://doi.org/10.1007/s42729-024-02002-6","url":null,"abstract":"<p>Vermicompost is an environment-friendly and effective organic fertilizer, and has been widely used to improve soil quality with different addition doses. However, the potential effects of vermicompost size that may affect soil porosity and nutrient release are rarely studied. Two sizes (0–2 mm and 3–5 mm) and four volume proportions (5%, 10%, 20%, and 30%) of vermicompost were added in the poor soil to improve soil fertility with the originally unimproved soil as the control (CT). Almost all physical parameters, nutrients, aggregates, and soil enzyme activity measured in this study were positively affected by vermicompost addition, and significantly related to addition proportion and vermicompost size. Overall, vermicompost addition decreased soil pH and bulk density, while increased electrical conductivity and porosity. The mean weight diameter (MWD) and geometric mean diameter (GMD) in the S8 (30% proportion, 3–5 mm size) were 15.2% and 9.1% higher, and the water stable aggregate (WSA_0.25) in the S4 (30% proportion, 0–2 mm size) was 27.7% higher than the CT. On average, the MWD and GMD in the bigger size group were 19.7% and 19.4% higher than those in the smaller size group. The activities of urease, saccharase, dehydrogenase, and glutamate decarboxylase in the S4 and S8 were 3.3 and 2.1 times, 2.9 and 4.9 times, 50.7% and 69.5%, 72.1% and 72.7% higher than the CT. Vermicompost addition increased the content of organic matter, available nitrogen, phosphorus, and potassium with the biggest enhancement in the S4 and S8 (ranging from 1.2 to 4.9 times), which was related to the increase in aggregates and enzyme activity. Vermicompost application had positive effects on soil improvement with addition proportion being more significant than vermicompost size.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Soil Carbon Sequestration with C-Rich Carrier Materials from Spent Mushroom Substrate and Composted Wheat Straw: Implications for Smart Fertilizer Design","authors":"Marcela Calabi-Floody, Jorge Medina, Alan E. Richardson, María Javiera Guarda-Reyes, Pamela Villegas-Pizarro, Isidora Zirotti-Lecaros, Lizeth Mora-Pérez, Humberto Aponte, Heike Knicker, Cornelia Rumpel, María de la Luz Mora","doi":"10.1007/s42729-024-01997-2","DOIUrl":"https://doi.org/10.1007/s42729-024-01997-2","url":null,"abstract":"<p>This study aimed to evaluate the potential of two carbon (C)-rich carrier materials derived from agricultural residues, spent mushroom substrate (SMS) and composted wheat straw (CWS) for enhancing soil carbon stabilization in an Andisol, with a view towards their future application in smart fertilizer design. We investigated how their contrasting nitrogen contents and application rates affect soil organic carbon dynamics and contribute to sustainable soil management and climate change mitigation. Soil incubations were conducted over 365 days, during which C mineralization, enzymatic activities, and C and N sequestration were assessed. SMS addition at both low and high rates (0.5% and 1% soil C increase, respectively) enhanced soil organic carbon (SOC) stabilization, increasing mean residence times (40.6 and 48.8 years) and half-life times (28.1 and 33.8 years) of the stable C pool compared to unamended soil (35.9 and 24.9 years). High-rate CWS application (1% soil C increase) promoted native SOC decomposition, increasing C losses (5.8%) and reducing C sequestration potential (96%). However, low-rate CWS application (0.5% soil C increase) showed promise, increasing mean residence time (46.8 years) and half-life time (32.4 years) of the stable C pool. Spearman correlations revealed positive associations between electrical conductivity, total N, humification indices, and C stabilization parameters, highlighting the importance of nutrient availability and humification potential for C stabilization. Incorporating C-rich carrier materials with balanced nutrient content, such as SMS, can enhance soil C stabilization and support climate-smart agriculture goals. Low-rate CWS application also shows potential as an alternative C-rich carrier material. However, careful consideration of application rates and material properties is crucial to avoid adverse effects on native SOC mineralization<i>.</i></p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon Sequestration by Potassium-Modified Bagasse Biochar in Manganese-Contaminated Sugarcane Field Soils","authors":"Yu Yang, Xuehui Liu, Ke Li, Haiping Luo, Lening Hu, Shuangli Li, Hua Deng","doi":"10.1007/s42729-024-02000-8","DOIUrl":"https://doi.org/10.1007/s42729-024-02000-8","url":null,"abstract":"<p>This study aims to investigate the transformation and carbon sequestration mechanisms of soil organic carbon in manganese-contaminated farmland. A 100-day constant temperature incubation experiment was conducted using potassium dihydrogen phosphate-modified bagasse biochar (BC-K) at concentrations of 0%, 0.5%, 2%, and 5%. The effects of BC-K on the mineralization of organic carbon and the changes in the physicochemical properties of manganese-contaminated soils were examined. The results demonstrated that applying 0.5%, 2%, and 5% BC-K to manganese-contaminated soils significantly reduced cumulative CO₂ emissions by 411.94 mg·kg^− 1, 47.33 mg·kg^− 1, and 105.24 mg·kg^− 1, respectively. The greatest reduction was observed with the 0.5% BC-K application compared to the control. The application of 2% and 5% BC-K to manganese-contaminated soil increased SOC by 121.50–165.23%, DOC by 24.46–30.05%, and MBC by 5.41 to 6.19 times. However, ROC decreased by 29.83–30.04%. In addition, the application of BC-K in manganese-contaminated soil can increase soil AP, AK, CEC, pH, and catalase. The application of BC-K can effectively reduce CO₂ emissions in manganese-contaminated farmland soil while significantly increasing soil organic carbon content and improving its physical and chemical properties. The findings of this study offer a scientific foundation for developing carbon sequestration and soil nutrient management strategies in manganese-contaminated farmland soils. These insights are crucial for enhancing soil environmental quality.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing pH for Soil Enzyme Assays Reveals Important Biochemical Functions in Low pH Soil","authors":"Tandra D. Fraser, Sarah Duddigan, Anita Diaz, Iain Green, Mark Tibbett","doi":"10.1007/s42729-024-01866-y","DOIUrl":"https://doi.org/10.1007/s42729-024-01866-y","url":null,"abstract":"<p>Soil enzyme assays are often used as indicators of potential biological functions. The objective of this study was to understand enzyme activity across a range of soil pH. Soils (0–15 cm) were collected from a heathland restoration project (established 1999) on the Isle of Purbeck, UK with treatments of elemental sulphur or ferrous sulphate compared to a control, acid grassland and heathland. Enzyme assays were conducted using fluorescent substrates for β-1,4-glucosidase, β-N-acetylglucosaminidase (NAG) and phosphatase with a range of buffer pH from 3.0 to 12.0. Differences in soil pH were still evident with the control (pH 5.3) and ferrous sulphate (pH 5.2) significantly higher than elemental sulphur (pH 4.5), acid grassland (pH 4.3) and heathland (pH 4.0). The optimum buffer pH for enzyme assays varied from pH 3-4.5 for β-glucosidase, pH 4–5 for NAG and pH 4–6 for phosphatase. Comparisons using a standard MUB pH resulted in different conclusions compared to optimum pH. For example, β-glucosidase activity at pH 5 for the control was significantly higher than elemental sulphur, acid grassland, and heathland. However, there were no differences when the pH optimums were considered. Comparisons of phosphatase activity at MUB pH 6.5 resulted in higher activity in the control plots compared to the heathland, despite the heathland soils showing the highest activity at optimum buffer pH. By examining the relationships between soil pH, enzyme activity, and assay conditions, this study highlights the importance of optimizing pH in enzyme assays when comparing diverse soil types.</p>","PeriodicalId":17042,"journal":{"name":"Journal of Soil Science and Plant Nutrition","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142190118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}