Journal of Research in Applied and Basic Medical Sciences最新文献

{"title":"Evaluation of Ampicillin- Lactose Incompatibility Reactions at Solid-State Using Physicochemical Methods","authors":"Amir Ali Babazadeh miyandoab, Leila Barghi, Nesa Yousefi, F. Ghaderi","doi":"10.52547/rabms.8.2.69","DOIUrl":"https://doi.org/10.52547/rabms.8.2.69","url":null,"abstract":"Background & Aims : One of the significant steps in the preformulation of pharmaceutical dosage forms is to examine active pharmaceutical ingredient (API) compatibility with the excipients applied with it. Drug-excipient incompatibility affects the stability, effectiveness, safety, and quality of the final product. Therefore, it is vital, for fruitful drug production, to sort out excipients compatible with the active pharmaceutical ingredient. The aim of this study was to evaluate the compatibility of ampicillin with lactose through differential scanning calorimetry (DSC) and Fourier-transform infrared spectroscopy (FTIR) physicochemical methods. Materials & Methods : To formulate powder samples, 300 mg ampicillin and lactose powder was discharged into a vial in a ratio of 1: 1, and 20% v/w water was added and then vortexed. Also, samples of pure drug and pure excipient were prepared by the same method. The direct compression method was applied to formulate the tablet samples. The samples were stored at 60° C in the oven and possible incompatibilities were examined for four consecutive weeks by observing the DSC thermograms and FTIR spectra. Results : According to the results, it is recommended to eschew the formulation of ampicillin with lactose for possible incompatibilities. Conclusion : Based on the obtained results and the changes in the main peaks of absorption of the drug-excipient mixture in the fourth week compared to the first day, there is the possibility of incompatibility in the mixture of ampicillin and lactose. Also, the observation of discoloration in the powder and tablets in the fourth week compared to the first day indicates the occurrence of a kind of incompatibility in the drug-excipient mixture.","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"201 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78489445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Antihypertensive Drugs on Placental Villi of Preeclamptic Patients: A Histopathological Study","authors":"Hamideh Ansari, Mohammad Faizan Ansari, Smrity Gupta, Medha Das","doi":"10.52547/rabms.8.2.64","DOIUrl":"https://doi.org/10.52547/rabms.8.2.64","url":null,"abstract":"Background & Aims : The placenta has been described as the most accurate record of the infant’s prenatal experience. The present study was a quantitative study and it was carried out to see the effect of pre-eclampsia on histopathology of placenta and the comparative changes in placenta of preeclamptic patients taking antihypertensives. Materials & Methods : A total of 60 patients were selected from the Obstetrics and Gynecology Department of Jawahar Lal Nehru Medical College & Hospital, AMU, Aligarh, UP, India and were grouped into control, untreated, and treated groups. Placenta of each group was fixed in 10% formalin solution and processed for section cutting. 5-micron thick sections were prepared and stained with Hematoxylin-eosin and Wiegerts & Van gieson stain and observed under the light of the microscope. A total of 200villi/hpf was counted and percentages for each parameter were expressed. Result : It has been shown that there was a significant reduction (<0.05) in the percentage of placental villi showing syncytial knot, cytotrophoblast proliferation, Basement membrane thickening, fibrinoid necrosis, and stromal fibrosis. Conclusion : It has been concluded that antihypertensive drugs were not only effective in controlling the symptoms of pre-eclampsia but also effective in minimizing the placental histopathology and helpful in continuation of pregnancy under low utero-placental flow without completely normalizing the placental changes.","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73202489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidrug Resistance among Clinical Isolates of Acinetobacter lwoffii","authors":"Shohreh Afsharyavari","doi":"10.52547/rabms.8.2.104","DOIUrl":"https://doi.org/10.52547/rabms.8.2.104","url":null,"abstract":"","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"178 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76775176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of platelet parameters in patients with non-hematological disorders","authors":"Priyanka Pappula, Vallamreddy Siva Kota Reddy, Vaheda Begam Korrapadu, Hima Bindu Gurram","doi":"10.52547/rabms.8.2.56","DOIUrl":"https://doi.org/10.52547/rabms.8.2.56","url":null,"abstract":"Background & Aims : People with diabetes, exhibit increased platelet reactivity. Both insulin resistance and insulin deficiency increase platelet reactivity. Platelets play a vital role in the pathogenesis of acute coronary syndromes (ACS). It has been shown that PC and MPV are independent predictors for poor outcome in primary intracerebral hemorrhage. The aim of the study is to analyze the platelet parameters in patients with some non-hematological disorders such as diabetes mellitus type 2, acute coronary syndrome, and acute ischemic stroke. Materials & Methods : Blood samples were collected from 400 subjects and analysed using the Sysmex KX-21 automated hematology analyser. 300 patients presenting with non-hematological disorders and 100 age and sex matched healthy controls were checked for Platelet indices such as PC, MPV, PDW and P-LCR. The platelet morphology was studied on peripheral blood smear for considering the arrangement, granularity and size of platelets. Results : In total non-hematological disorders, DM type 2 accounts 37.33% (n=112), followed by 35.67% of ACS (n=107), and 27% of AIS (n=81). In diabetes mellitus type 2, PC was statistically elevated along with MPV compared with healthy controls. MPV and PDW were significantly raised in the patients admitted with ACS. In acute ischemic stroke, we noted that there was an elevation in MPV and a decrease in PC compared to them in the control group. Study showed discrete arrangement of platelets in 90% of DM type 2, 88% of ACS, and 80% of AIS patients. PBS in various clinical conditions showed granularity of platelets in 84 % of DM type 2, 81% of ACS and 83% of AIS patients. Study showed increased percentage of macrothrombocytes as 60% of DM type 2, 56% of ACS and 52% of AIS patients. The percentage of large platelets on PBS was increased beyond normal limits in DM type 2, ACS and AIS cases, indicating that there is an increase in size of the platelets supporting an increase in MPV. Conclusion : The measurement of platelet indices may provide useful diagnostic and prognostic information to emergency physicians caring for patients with ACS and AIS.","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76437464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of serum lipid profile in patients with oral submucous fibrosis and oral squamous cell carcinoma and their comparative analysis","authors":"Sangeeta Khyalia","doi":"10.52547/rabms.8.2.90","DOIUrl":"https://doi.org/10.52547/rabms.8.2.90","url":null,"abstract":"Background & Aims : The aim of this study was to evaluate serum lipid profile and compare their levels between the patients with oral submucous fibrosis (OSMF) and oral squamous cell carcinoma (OSCC). Materials & Methods : This descriptive study was done in two groups of patients; OSMF and OSCC patients. There were forty-five participants in each group. Serum samples obtained from centrifugation of 12 hour fasting blood samples was analyzed by fully automated analyzer Beckman coulter AU-680 for estimation of the lipid levels (cholesterol, triglycerides (TGL), and high‑density lipids (HDL)) using colorimetric methods. Low‑density lipid (LDL) values were obtained by calculation. Results : The comparison of lipid profile between Oral Submucous Fibrosis and Oral Carcinoma cases shows statistically significant results for TC, HDL and LDL. Conclusion : The change in lipid levels may have an early diagnostic or prognostic role in oral premalignant and malignant lesions.","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90717413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prevalence of Impacted Third Molar, Impaction Angulation, and Impaction Depth in Patients Visiting Dental Clinics and Private Offices in Ghaemshahr, Iran, in 2016","authors":"Seyed Mehdi Taghain, A. Maboudi, Mahmoud Goli, A. Sameie, L. Sadri","doi":"10.52547/rabms.8.2.83","DOIUrl":"https://doi.org/10.52547/rabms.8.2.83","url":null,"abstract":"Background & Aims : The present study aimed to determine the prevalence of impacted third molar as well as impaction angulation and depth. Materials & Methods : In this cross-sectional descriptive study, 261 panoramic radiographs belonging to patients visiting dental clinics and offices in Ghaemshahr, Iran, were evaluated and the presence of impacted wisdom teeth was examined. Moreover, the angulation of impacted teeth, impaction depth, and the relationship of the tooth to the mandibular ramus were recorded. The data were recorded, collected, and statistically analyzed in SPSS 22 using the non-parametric chi-square test. p <0.05 was considered significant. Results : Of the 261 patients entering the study, 52 (17.69%) had at least one impacted tooth. Of the total number of patients with impacted teeth, 31 were women (mean prevalence of 19.87% of the total population of women) and 21 were men (15.22% of the total population of men), showing no significant difference (p=0.29). In terms of impaction depth based on Pell and Gregory’ classification, Class C impaction depth was the most prevalent in the maxilla, while Class A was the most prevalent in the mandible. The most prevalent impaction in terms of angulation in relation to the second molar was vertical in the maxilla and vertical and mesioangular in the mandible. Conclusion : Based on results, the prevalence of impacted wisdom teeth in patients was 17.69%. This may not be a striking amount, but it is still of significance since the possible complications of impacted teeth are costly and problematic.","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77471569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perception of COVID-19 vaccine due to media influence and its impact","authors":"Somya Thakan, Aditya Mehta, Shakila Mulla","doi":"10.52547/rabms.8.2.97","DOIUrl":"https://doi.org/10.52547/rabms.8.2.97","url":null,"abstract":"Background & Aims : Social media/mobile mass communications, while providing an unprecedented capacity for the masses to communicate, has also been a major drivers in the rise of fringe opinions very damaging to public health. Reconciling principles of freedom of speech with the usage of social media for damaging falsehoods remains a conundrum for nations. Hence it was desired to conduct a study with objective of determining the influence of social media on the perception of COVID-19 vaccine. Materials & Methods : An analytical cross-sectional study was conducted from May - July 2021 (duration three months) on those who gave consent and were eligible for vaccination according to the guidelines by ICMR (Indian council of medical research) and GOI (Government of India) Result : The majority of study participants 76.7% were aware of covid appropriate behaviour and preventive measures and around 61.8% of participants were aware of covid vaccination. The vast majority of the participants stated that they had obtained information about vaccinations (61.8%). The preferred method for acquiring information was social media (56.6%), followed by community health workers (15.6%), media-internet/news/television (15.3%) and very little importance to national guidelines through miking, pamphlets (5.6%). Conclusion: The impact of social media on the influence of covid vaccination was very disturbingly negative as a vast majority of study participants 60.7% tried to convince people to avoid vaccines after hearing about adverse events following vaccination through the social media platform.","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87861706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Three Hemoglobin Estimation Methods for Screening Blood Donors: An Experience in a South Indian Hospital","authors":"Jhansi Priya Gaddam Harikrishna, Yashovardhan Araveti, Amulya Boddapati","doi":"10.52547/rabms.8.1.1","DOIUrl":"https://doi.org/10.52547/rabms.8.1.1","url":null,"abstract":"Background & Aims : Hemoglobin (Hb) estimation is one of the mandatory tests should be carried out on all blood donors before accepting for blood donation to prevent collecting blood samples from anemic donors. Presently various methods are available for pre-donation screening of donor hemoglobin. Total Hb concentration is calculated from measured absorbance at multiple wavelengths. In this study we reviewed the performance of Compolab TS with automated SYSMEX XN 1000i hematology analyzer and with standard cyanmethemoglobin method. Materials & Methods : This is a prospective study carried out in the department of Transfusion Medicine, Narayana Medical College and Hospital, India. A total of 466 blood donors were screened and evaluated for hemoglobin estimation by three different methods. Comparison and their test performances were evaluated by analysis of coefficients of variation (CV), linear regression, and mean differences, using SPSS Software version 20.0. Results : There was no difference between the mean Hb determinations. The CVs for three methods Compolab TS, 0Coulter, and Cyanmethemoglobin methods were 0.71%, 0.44%, and 0.85%, respectively. Compolab TS and Coulter showed best agreement and the Cyanmethemoglobin showed lower determination rate compared to both the Coulter and Compolab TS tests. However, pair methods involving Cyanmethemoglobin seem to narrower limits of agreement (± 0.1636 and ± 0.2152) than Compolab TS and Coulter combination (± 0.2730). Conclusion : Estimation of Hb by Compolab TS is rapid, simple and easy and showed good agreement with the standard methods.","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"13 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91420443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of e-learning during covid-19 pandemic: a cross sectional survey among health sciences students in northern India","authors":"Shweta M. Chaudhary, Rishi Kumar Bharti, Swati Yadav, Rinku Garg","doi":"10.52547/rabms.8.1.44","DOIUrl":"https://doi.org/10.52547/rabms.8.1.44","url":null,"abstract":"Background & Aims : To prepare qualified doctors for today’s environment in which the internet provides universal digital information, the teaching methods used for educating and training medical school students should be reconsidered for their effectiveness. The aim of this study was to investigate effectiveness of online teaching in facilitating medical education during the COVID-19 pandemic in northern India. Materials & Methods : This Cross-sectional, online survey study was conducted on total 334 students of 18-22 years' age by giving questionnaire which consisted of 10 questions. Informed consent was also taken. Questionnaire was given through online Google forms and link shared through social media and responses were collected. Questions were 5-point Likert-type questions, ranging from strongly disagree to strongly agree. Responses were collected in a one-week period. Statistical analysis was done using MS Excel program (ver. 2019, Microsoft, Redmond, WA, USA). Results : It was shown that 36.5% of the students disagree for “effectiveness of online teaching whereas 69.9% of them agree for “preference for online teaching to offline teaching. The commonly perceived disadvantages as perceived by students to using online teaching platforms were problematic internet connection (42.5%) and lack of two-way interaction. (22.2%). P values calculated for mean of paramedical and medical group was 0.03, which was statistically significant . Conclusion : Our study results showed that 82.6% of the students agreed that online teaching has not successfully replaced the offline teaching. Whereas 91.5% of the students felt they could not learn practical skills through online teaching. This indicates practical skills remain as potential disadvantage for online teaching.","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"C-27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84435055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Platelet Parameters and their Prognostic Significance in Anemia and Leukemia","authors":"Siva Kota Reddy Vallamreddy, Priyanka Pappula, Vaheda Begam Korrapadu","doi":"10.52547/rabms.8.1.36","DOIUrl":"https://doi.org/10.52547/rabms.8.1.36","url":null,"abstract":"Background & Aims : Most commonly assessed Platelet indices include the mean platelet volume (MPV), platelet distribution width (PDW), platelet-large cell ratio (P-LCR), and the plateletcrit (PCT). The platelet behavior is often unpredictable and complicated in Iron Deficiency Anemia. High MPV and low PDW were reported in patients with leukemia. The present study aimed to evaluate the significance of platelet parameters in anemia and leukemia cases. Materials & Methods : A cross-sectional and observational case-control study was carried out at a teaching hospital of Andhra Pradesh. We measured the platelet indices using an automated counter. Laboratory data from 200 patients of anemia and leukemias were analysed. Results : Hematological disorders such as anemia in 168 cases and Leukemia in 32 cases were recorded. Platelet parameters specifically platelet count (PC), MPV, PDW, and P-LCR were significantly lowered in acute leukemia patients than them of the control group. In chronic leukemia (both CML & CLL) patients, all the platelet parameters such as mean platelet component (MPC), MPV, PDW, and P-LCR were found to be higher than them of the control group. In the majority of chronic leukemia cases, platelets on PBS were discrete (81%) and hypogranular (55%). Inverse relationship noted between MPV and PC among anemic patients. PC in acute leukemia (both AML & ALL) patients was lower than that of the control group. PDW was significantly lower in acute leukemia patients compared to that in the control group. P-LCR was also found to be significantly lower in the acute leukemia group compared to the control group. The PC was higher and the MPV was lower in the anemic group compared to the control group. Conclusion : In chronic leukemia patients, all the platelet parameters such asPC, MPV, PDW, and P-LCR were found to be significantly higher than them of the control group. PC was higher and the MPV was lower in the anemic group compared to the control group. All the platelet parameters such as MPV, PC, PDW, and P-LCR were significantly lower in acute leukemia patients compared to them in the control group, in contrast with chronic leukemia patients where the parameters were significantly higher compared to them in the control group.","PeriodicalId":16970,"journal":{"name":"Journal of Research in Applied and Basic Medical Sciences","volume":"8 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90252820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}