Journal of Reproductive Immunology最新文献

{"title":"The interplay between extracellular NAMPT and inflammatory cytokines in preeclampsia","authors":"Priscila Rezeck Nunes ,&nbsp;Daniela Alves Pereira ,&nbsp;Luis Fernando Pereira Passeti ,&nbsp;Lídia Lana Ferreira Coura ,&nbsp;Karina Braga Gomes ,&nbsp;Valeria Cristina Sandrim ,&nbsp;Marcelo Rizzatti Luizon","doi":"10.1016/j.jri.2024.104248","DOIUrl":"10.1016/j.jri.2024.104248","url":null,"abstract":"<div><p>Preeclampsia (PE) is the major cause of maternal-fetal mortality and morbidity. Its pathophysiology is not elucidated, but there is evidence for the role of visfatin/nicotinamide phosphoribosyl transferase (NAMPT), mainly due to its relation to endothelial dysfunction, a hallmark of PE. However, there is heterogeneous data regarding visfatin/NAMPT in healthy pregnancy (HP) and PE. Therefore, we performed a search on MEDLINE/PubMed using the terms “visfatin and preeclampsia” and “NAMPT and preeclampsia, and we selected 23 original articles: 12 articles reported increased levels in PE compared to HP, only four articles showed lower levels and eight articles did not find differences regarding visfatin/NAMPT in the groups studied. It is widely acknowledged that levels detected in plasma, serum, or placenta can be influenced by the size of the population and sample analyzed, as well as genetic factors. We further discussed the correlations of visfatin/NAMPT with clinical biomarkers in PE and inflammatory pathways. Considering the common inflammatory mechanisms between PE and visfatin/NAMPT, few studies have recently performed serum or plasma dosages. In conclusion, further studies are needed to highlight the potential role of visfatin/NAMPT in the pathophysiology of PE. This will provide comparative evidence to establish it as a biomarker for disease outcomes and treatment.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104248"},"PeriodicalIF":3.4,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140780406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of female bone marrow transplantation on male reproductive organs","authors":"Tatsuhiko Takahashi ,&nbsp;Kenta Nagahori ,&nbsp;Takuya Omotehara ,&nbsp;Miyuki Kuramasu ,&nbsp;Yuki Ogawa ,&nbsp;Xi Wu ,&nbsp;Yutaro Natsuyama ,&nbsp;Shinichi Kawata ,&nbsp;Tomiko Yakura ,&nbsp;Hidenobu Miyaso ,&nbsp;Zhong-Lian Li ,&nbsp;Masahiro Itoh","doi":"10.1016/j.jri.2024.104245","DOIUrl":"https://doi.org/10.1016/j.jri.2024.104245","url":null,"abstract":"<div><p>Graft-versus-host disease (GVHD), an adverse effect after bone marrow transplantation (BMT), may affect male reproductive function. It is hypothesized that a sex-mismatched BMT induces GVHD in male reproductive organs because female immune cells are not immunologically tolerant to specific antigens of the male organs. However, this hypothesis has not been experimentally verified using male (M) recipient animals following BMT from the female (F) donors. Therefore, the aim of the present study is to examine whether the female BMT to males (F→M group) induces some GVHD reactions in the testis and the other male reproductive organs. The results showed that no inflammation was found in recipients of the male BMT to males (M→M group), whereas significant inflammatory cell responses lasting for at least 4 months were induced in testis, epididymis, prostate and preputial gland in some mice of F→M group. The most severe lesion was found in the preputial gland, in which lymphocytic inflammation was accompanied by loss of glandular acini, thickening of the interstitum and increased cytokines such as TNF-α and IFN-γ. Western blot analyses revealed that sera from the F→M group reacted with various antigens of the male reproductive organs. These results indicate that transplanted female immune cells may recognize the male reproductive organs as immunologically foreign ones and induce chronic GVHD, which may affect male reproductive function.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104245"},"PeriodicalIF":3.4,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165037824000548/pdfft?md5=6e7feac4f99c377d39b965f6977e9357&pid=1-s2.0-S0165037824000548-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140543628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progesterone-mediated remodeling of the maternal-fetal interface by a PGRMC1-dependent mechanism","authors":"Fang Wang ,&nbsp;Leonardo M.R. Ferreira ,&nbsp;Andrew Mazzanti ,&nbsp;Huaxiao Yu ,&nbsp;Bowen Gu ,&nbsp;Torsten B. Meissner ,&nbsp;Qin Li ,&nbsp;Jack L. Strominger","doi":"10.1016/j.jri.2024.104244","DOIUrl":"10.1016/j.jri.2024.104244","url":null,"abstract":"<div><p>Implantation and maintenance of pregnancy involve intricate immunological processes that enable the developing fetus to coexist with the maternal immune system. Progesterone, a critical hormone during pregnancy, is known to promote immune tolerance and prevent preterm labor. However, the mechanism by which progesterone mediates these effects remains unclear. In this study, we investigated the role of the non-classical progesterone receptor membrane component 1 (PGRMC1) in progesterone signaling at the maternal-fetal interface. Using JEG3 cells, a trophoblast model cell line, we observed that progesterone stimulation increased the expression of human leukocyte antigen-C (HLA-C) and HLA-G, key molecules involved in immune tolerance. We also found that progesterone upregulated the expression of the transcription factor ELF3, which is known to regulate trophoblast-specific HLA-C expression. Interestingly, JEG3 cells lacked expression of classical progesterone receptors (PRs) but exhibited high expression of PGRMC1, a finding we confirmed in primary trophoblasts by mining sc-RNA seq data from human placenta. To investigate the role of PGRMC1 in progesterone signaling, we used CRISPR/Cas9 technology to knockout PGRMC1 in JEG3 cells. PGRMC1-deficient cells showed a diminished response to progesterone stimulation. Furthermore, we found that the progesterone antagonist RU486 inhibited ELF3 expression in a PGRMC1-dependent manner, suggesting that RU486 acts as a progesterone antagonist by competing for receptor binding. Additionally, we found that RU486 inhibited cell invasion, an important process for successful pregnancy, and this inhibitory effect was dependent on PGRMC1. Our findings highlight the crucial role of PGRMC1 in mediating the immunoregulatory effects of progesterone at the maternal-fetal interface.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104244"},"PeriodicalIF":3.4,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140268663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-eclampsia: Re-visiting pathophysiology, role of immune cells, biomarker identification and recent advances in its management","authors":"Palanisamy Tamil Barathi,&nbsp;Arumugam Mohanapriya","doi":"10.1016/j.jri.2024.104236","DOIUrl":"https://doi.org/10.1016/j.jri.2024.104236","url":null,"abstract":"<div><p>Pre-eclampsia (PE) is a hypertension condition that occurs exclusively during pregnancy and has the potential to impact nearly all organ systems. It is estimated to complicate approximately 2–8% of pregnancies worldwide. PE is a prominent medical disorder that poses a significant risk to pregnant mothers and their infants. This review commences by giving the most up-to- date concepts about the pathophysiology of PE. The condition involves atypical infiltration of trophoblast cells into the spiral arteries of the decidua and myometrium, resulting in an insufficient establishment of proper blood flow between the uterus and placenta. The aberrant activation of natural killer (NK) cells in both the peripheral blood and the decidua has been identified as one of the contributing factors to the development of PE. The strong evidence for the genetic etiology of PE is provided by the association between maternal killer cell immunoglobulin-like receptor (KIR) and Human Leukocyte Antigen (HLA-C) in trophoblast cells. Recent observations provide evidence that changes in the expression of anti-angiogenic factors in the placenta are the underlying cause of the clinical symptoms associated with the condition. This review also provides a comprehensive overview of the latest advancements in understanding the underlying causes of PE. It specifically highlights the emergence of new diagnostic biomarkers and their potential implications for therapeutic interventions in managing this medical condition.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104236"},"PeriodicalIF":3.4,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140328665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics and functions of memory regulatory T cells in normal pregnancy cycle and pregnancy complications","authors":"Zeyang Chen ,&nbsp;Xiaojiao Zhou ,&nbsp;Hongmei Qu ,&nbsp;Xiaolu Zhang ,&nbsp;Joanne Kwak-Kim ,&nbsp;Wenjuan Wang","doi":"10.1016/j.jri.2024.104235","DOIUrl":"10.1016/j.jri.2024.104235","url":null,"abstract":"<div><p>Regulatory T cells (Tregs) are activated and expanded after exposure to fetal-specific (paternal) antigens. A proportion of Tregs differentiate into memory Tregs (mTregs), exhibiting immune memory function and exerting more potent immunosuppression than naive Tregs (nTregs). However, it is unclear how mTregs are regulated during normal and pathological pregnancies (e.g., gestational diabetes mellitus (GDM) and preeclampsia (PE)). In this study, PD-1, HLA-G, and HLA-DR expressions on memory CD4⁺ T cells, naive CD4⁺ T cells, Tregs, mTregs, and nTregs in healthy non-pregnant women (n=20), healthy first (n=20), second (n=20), and third-trimester women (n=20), postpartum women (n=20), GDM (n=20), and PE patients (n=20) were analyzed. The proportion of mTregs out of Tregs was increased (P&lt;0.05) in the first trimester compared with that in non-pregnancy and reduced in the second and third trimesters. The proportions of PD-1⁺ Tregs and mTregs were significantly increased during the first trimester compared to those of non-pregnancy (P&lt;0.01), reached their maximum in the second trimester. Moreover, the proportions of HLA-G⁺ memory CD4⁺ T cells, Tregs, and mTregs were increased in the first and second trimesters (P&lt;0.01), reached their maximum in the third trimester. GDM patients were characterized by significantly lower percentages of PD-1⁺ and HLA-G⁺ mTregs (P&lt;0.01), while PE patients were characterized by significantly lower percentages of HLA-G⁺ mTregs (P&lt;0.01), compared with the healthy third-trimester women. In general, as demonstrated by this study, mTregs increase in number and enhance maternal-fetal immunoregulation during pregnancy, and their dysfunction can result in pregnancy complications such as GMD or PE.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104235"},"PeriodicalIF":3.4,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140181768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 infection, inflammation and birth outcomes in a prospective NYC pregnancy cohort","authors":"Frederieke A.J. Gigase ,&nbsp;Rebecca H. Jessel ,&nbsp;Elianna Kaplowitz ,&nbsp;Natalie Boychuk ,&nbsp;Sophie Ohrn ,&nbsp;Erona Ibroci I ,&nbsp;Juliana Castro ,&nbsp;Jezelle Lynch ,&nbsp;Rushna Tubassum ,&nbsp;Amy Balbierz ,&nbsp;Nina M. Molenaar ,&nbsp;Mara Graziani ,&nbsp;Roy Missall ,&nbsp;Tammy Flores ,&nbsp;Toni Stern ,&nbsp;Juan Manuel Carreno ,&nbsp;Krammer Serology Core Study Group ,&nbsp;Florian Krammer ,&nbsp;Alan Adler ,&nbsp;Rachel I. Brody ,&nbsp;Teresa Janevic","doi":"10.1016/j.jri.2024.104243","DOIUrl":"10.1016/j.jri.2024.104243","url":null,"abstract":"<div><p>Associations between antenatal SARS-CoV-2 infection and pregnancy outcomes have been conflicting and the role of the immune system is currently unclear. This prospective cohort study investigated the interaction of antenatal SARS-CoV-2 infection, changes in cytokine and HS-CRP levels, birthweight and gestational age at birth. 2352 pregnant participants from New York City (2020–2022) were included. Plasma levels of interleukin (IL)-1β, IL-6, IL-17A and high-sensitivity C-reactive protein (HS-CRP) were quantified in blood specimens obtained across pregnancy. Quantile and linear regression models were conducted to 1) assess the impact of antenatal SARS-CoV-2 infection, overall and by timing of detection of SARS-CoV-2 positivity (&lt; 20 weeks versus <span><math><mo>≥</mo></math></span> 20 weeks), on birthweight and gestational age at delivery; 2) examine the relationship between SARS-CoV-2 infection and maternal immune changes during pregnancy. All models were adjusted for maternal demographic and obstetric factors and pandemic timing. Birthweight models were additionally adjusted for gestational age at delivery and fetal sex. Immune marker models were also adjusted for gestational age at specimen collection and multiplex assay batch. 371 (15.8%) participants were infected with SARS-CoV-2 during pregnancy, of which 98 (26.4%) were infected at &lt; 20 weeks gestation. Neither SARS-CoV-2 infection in general nor in early or late pregnancy was associated with lower birthweight nor earlier gestational age at delivery. Further, we did not observe cytokine or HS-CRP changes in response to SARS-CoV-2 infection and thus found no evidence to support a potential association between immune dysregulation and the diversity in pregnancy outcomes following infection.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104243"},"PeriodicalIF":3.4,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165037824000524/pdfft?md5=1d3b951978342027d0f80f4a68a2ae3c&pid=1-s2.0-S0165037824000524-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140181939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune cells at the feto-maternal interface: Comprehensive characterization and insights into term labor","authors":"Angela Mosebarger ,&nbsp;Manuel S. Vidal Jr. ,&nbsp;Giovana Fernanda Cosi Bento ,&nbsp;Ryan C.V. Lintao ,&nbsp;Mary Elise L. Severino ,&nbsp;Ananth kumar Kammala ,&nbsp;Ramkumar Menon","doi":"10.1016/j.jri.2024.104239","DOIUrl":"https://doi.org/10.1016/j.jri.2024.104239","url":null,"abstract":"<div><p>Immune cells at the feto-maternal interface play an important role in pregnancy; starting at implantation, maintenance of pregnancy, and parturition. The role of decidual immune cells in induction of labor still needs to be understood. Published reports on this topic show heterogeneity in methods of cell isolation, assay, analysis and cellular characterization making it difficult to collate available information in order to understand the contribution of immune cells at term leading to parturition. In the present study, available literature was reviewed to study the differences in immune cells between the decidua basalis and decidua parietalis, as well as between immune cells in term and preterm labor. Additionally, immune cells at the decidua parietalis were isolated from term not in labor (TNL) or term in labor (TL) samples and characterized via flow cytometry using a comprehensive, high-dimensional antibody panel. This allowed a full view of immune cell differences without combining multiple studies, which must include variation in isolation and analysis methods, for more conclusive data. The ratio of cells found in decidua parietalis in this study generally matched those reported in the literature, although we report a lower percentage of natural killer (NK) cells at term. We report that CD4 expression on CD8^- NK cells decreased in term labor compared to not in labor samples, suggesting that natural killer cells may be migrating to other sites during labor. Also, we report a decrease in CD38 expression on CD8⁺ CD57⁺ T cells in labor, indicative of cytotoxic T cell senescence. Our study provides a comprehensive status of immune cells at the decidua-chorion interface at term.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104239"},"PeriodicalIF":3.4,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140138218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of innate and adaptive immunity in endometriosis","authors":"Khaleque N. Khan ,&nbsp;Sun-Wei Guo ,&nbsp;Kanae Ogawa ,&nbsp;Akira Fujishita ,&nbsp;Taisuke Mori","doi":"10.1016/j.jri.2024.104242","DOIUrl":"https://doi.org/10.1016/j.jri.2024.104242","url":null,"abstract":"<div><p>The innate and adaptive immune systems are the two key branches that determine host protection at all mucosal surfaces in human body, including the female reproductive tract. The pattern recognition receptors within the host that recognize pathogen-associated molecular patterns are expressed on the cells of the innate immune system. Rapidly reactive, theinnate immune system, responds immediately to the presence of infectious or other non-self agents, thereby launching an inflammatory response to protect the host until the activation of slower adaptive immune system. Macrophages, dendritic cells, and toll-like receptors are integral components of the innate immune system. In contrast, T-helper (Th1/Th2/Th17) cells and regulatory T (Treg) cells are the primary components of adaptive immune system. Studies showed that the growth and progression of endometriosis continue even in unilateral ovariectomized animal suggesting that besides ovarian steroid hormones, the growth of endometriosis could be regulated by innate/adaptive immune systems in pelvic environment. Recent reports demonstrated a potential role of Th1/Th2/Th17/Treg cells either individually or collectively in the initiation, maintenance, and progression of endometriosis. Herewe review the fundamental knowledge of innate and adaptive immunity and elaborate the role of innate and adaptive immunity in endometriosis based on both human and experimental data.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104242"},"PeriodicalIF":3.4,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140160938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental expression of inflammatory Galectin-12 is associated with gestational diabetes","authors":"Christina Buschmann ,&nbsp;Laura Unverdorben ,&nbsp;Julia Knabl ,&nbsp;Stefan Hutter ,&nbsp;Sarah Meister ,&nbsp;Susanne Beyer ,&nbsp;Maximiliane Burgmann ,&nbsp;Alaleh Zati zehni ,&nbsp;Elisa Schmoeckel ,&nbsp;Mirjana Kessler ,&nbsp;Udo Jeschke ,&nbsp;Tanja K. Eggersmann ,&nbsp;Sven Mahner ,&nbsp;Thomas Kolben ,&nbsp;Franziska Ganster","doi":"10.1016/j.jri.2024.104240","DOIUrl":"https://doi.org/10.1016/j.jri.2024.104240","url":null,"abstract":"<div><h3>Objectives</h3><p>Gestational diabetes mellitus (GDM) is a growing health concern. Since members of the galectin-family are identified to play a role in the pathogenesis of GDM, we determined galectin-12 as an essential protein due to its influence in lipolysis and inflammation processes. This study investigates the expression of galectin-12 in the placentas of women with GDM.</p></div><div><h3>Study design</h3><p>The study population includes 40 expectant women suffering from GDM and 40 healthy controls. The expression of galectin-12 in the syncytiotrophoblast (SCT) and the extra villous trophoblast (EVT) of the placenta was analyzed by immunohistological staining and double immunofluorescence. Immunoreactivity Score (IRS) was used for evaluation.</p></div><div><h3>Results</h3><p>The results demonstrate a significant overexpression of galectin-12 in the nucleus of the SCT and the EVT of placentas with GDM compared to the healthy control group.</p><p>Additionally, double immunofluorescence visualizes corresponding results with an overexpression of galectin-12 in the extra villous trophoblast of GDM placentas representing maternal cells.</p></div><div><h3>Conclusion</h3><p>This study identifies galectin-12 to be associated with the process of gestational diabetes mellitus. These findings are in correspondence with the involvement of galectin-12 in inflammatory processes. Maternal BMI and male sex seem to be confounder for the expression of galectin-12 in the nuclear syncytiotrophoblast, but not in other parts of the investigated placental areas. Further investigations are necessary to verify the correlation between gestational diabetes mellitus and the expression of galectin-12 in the placenta and to further elucidate its distinct role.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104240"},"PeriodicalIF":3.4,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165037824000494/pdfft?md5=315571ba743c95ec68a6025bf6d8ca51&pid=1-s2.0-S0165037824000494-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140138217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential tools for predicting response to chemotherapy in OC: Assessment of immune dysbiosis, participant’s self-rated health and microbial dynamics","authors":"Taylor Badger ,&nbsp;Elizabeth Anderson ,&nbsp;Sarah Nelson ,&nbsp;Kathleen Groesch ,&nbsp;Teresa Wilson ,&nbsp;Paula Diaz-Sylvester ,&nbsp;Kristin Delfino ,&nbsp;Nhung Le ,&nbsp;Laurent Brard ,&nbsp;Andrea Braundmeier-Fleming","doi":"10.1016/j.jri.2024.104241","DOIUrl":"10.1016/j.jri.2024.104241","url":null,"abstract":"<div><p>Epithelial ovarian cancer (OC) is the deadliest female reproductive cancer; an estimated 13,270 women will die from OC in 2023. Platinum-based chemotherapy resistance mechanisms contribute to poor OC 5-year survival rates. Peripheral inflammation is linked to various disease states and we previously identified unique peritoneal microbial features predictive of OC. We hypothesized that unique peripheral immune profiles and peritoneal microbial features may be predictive of disease-free interval (time to recurrence) and response to chemotherapy in participants with OC. We also investigated self-rated health (SRH) scores in the context of peripheral inflammation as a potential screening tool for OC. Blood and peritoneal fluid were collected from participants with OC or a benign adnexal mass (BPM). Lymphocyte populations were analyzed using Fluorescence Activated Cell Sorting, serum cytokine levels were analyzed using the Human Th17 Magnetic Bead Panel assay and peritoneal fluid microbial features were analyzed using Next Generation Sequencing (NGS). Participants completed a standardized questionnaire on self-rated physical and emotional health. Participants were classified into three chemotherapy response categories: platinum-refractory, platinum-resistant or platinum-sensitive. A significant positive correlation was found between elevated inflammatory status on the day of surgery and longer disease-free interval. SRH measures did not correlate with immune status in participants with OC or a BPM. We identified a correlation between peritoneal microbial features and chemotherapy response. We conclude that immune dysbiosis may be useful in predicting OC recurrence. The immune findings reported here set the framework for additional studies utilizing immune profiles to predict platinum-based chemotherapy responsiveness in OC.</p></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"163 ","pages":"Article 104241"},"PeriodicalIF":3.4,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140127097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}