Journal of Reproductive Immunology最新文献

{"title":"Gonadotropin-releasing hormone agonists combined with hormone replacement therapy improves the live birth rate of patients with thin endometrium and cured chronic endometritis.","authors":"Longlong Wei, Bing Tian, Shuna Wang, Siyue Xu, Weiran Hu","doi":"10.1016/j.jri.2025.104739","DOIUrl":"https://doi.org/10.1016/j.jri.2025.104739","url":null,"abstract":"<p><strong>Background: </strong>Both thin endometrium and chronic endometritis (CE) have adverse effects on pregnancy outcomes. Recently, it is often believed that downregulating gonadotropin-releasing hormone agonists (GnRH-a) might enhance endometrial receptivity and raise the chance of a successful pregnancy.</p><p><strong>Methods: </strong>A retrospective analysis of 2102 infertile women who received frozen embryo transfer (FET) cycles was carried out. Standard antibiotic treatment was administered to women with CE, and a follow-up biopsy verified that the CE had been cured. Subsequently, these patients received endometrial preparation, and FET was performed. This study systematically evaluated the impact of three different endometrial preparation strategies on pregnancy outcomes in FET cycles. Potential confounders were controlled through 1:1:1 propensity score matching (PSM) and multivariable logistic regression analysis based on prematched data.</p><p><strong>Results: </strong>There were 117 cycles in each group after matching and all baseline characteristics were balanced with no significant differences between the groups. In patients with thin endometrium and cured CE, the LBR in the GnRH-a-HRT group was significantly increased compared to both the HRT group and the NC group. The results after matching were highly consistent with the multivariable-adjusted findings from the pre-matching analysis.</p><p><strong>Conclusion: </strong>This study indicates that GnRH-a-HRT protocol improves the LBR in patients with thin endometrium and cured CE compared to the HRT and NC protocols in FET cycles.</p>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"104739"},"PeriodicalIF":2.9,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145301403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autoantibody-mediated disruption of the epidermal growth factor system during implantation and pregnancy.","authors":"Aiko Aoki, Toshitaka Sugi, Kei Kawana","doi":"10.1016/j.jri.2025.104736","DOIUrl":"https://doi.org/10.1016/j.jri.2025.104736","url":null,"abstract":"<p><p>Japan has a relatively high incidence of coagulation factor XII (FXII) and protein S deficiencies, which are undeniable risk factors for recurrent pregnancy loss (RPL). FXII and protein S share common epidermal growth factor (EGF)-like domains. Patients with RPL who have protein S and FXII deficiencies carry anti-protein S and anti-FXII antibodies (anti-FXII), respectively. Epitope mapping of these antibodies revealed that they have EGF-like domain epitopes, which cross-react with EGF. EGF plays a role in cell growth and differentiation, and its effects on the reproductive system, particularly in implantation and placental development, are well documented. We developed an anti-EGF antibody detection system to investigate the correlation between anti-EGF antibodies (anti-EGF) and other RPL-associated risk factors. Anti-EGF demonstrated a correlation not only with anti-FXII and anti-protein S but also with anti-phosphatidylserine/prothrombin antibodies (aPS/PT). Although the aPS/PT epitope lies within the thrombin region, which lacks an EGF-like domain, it nevertheless cross-reacts with EGF, indicating their pathogenicity to the EGF system as well as anti-FXII and anti-protein S. This review summarizes the history of antibody discovery and epitope mapping studies and discusses the pathogenic potential of these antibodies in pregnancy morbidity through their effects on reproductive biology.</p>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"104736"},"PeriodicalIF":2.9,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145292591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activated CD8(+) tissue-resident memory T cells impact the endometrial receptivity in minimal/mild endometriosis","authors":"Xin Huang ,&nbsp;Yuchan Zhong ,&nbsp;Hong Li ,&nbsp;Lukanxuan Wu ,&nbsp;Yujing Li ,&nbsp;Ruiying Wang ,&nbsp;Xinyu Qiao ,&nbsp;Yixian Han ,&nbsp;Hanyun Zhang ,&nbsp;Dong Liu ,&nbsp;Tianjiao Pei ,&nbsp;Huili Zhu ,&nbsp;Yunwei Ouyang ,&nbsp;Wei Huang","doi":"10.1016/j.jri.2025.104737","DOIUrl":"10.1016/j.jri.2025.104737","url":null,"abstract":"<div><div>Endometrial immune disorders create an inhospitable endometrial environment for embryonic nidation in endometriosis. CD8 + tissue resident memory T cells (CD8 +TRM) are abundant tissue resident immune cells in endometrium, but the effect of CD8 +TRM on endometrial receptivity of endometriosis remains unexplored. We collected endometrium tissue to explore the number and function of CD8 +TRM in eutopic endometrium of endometriosis and controls through flow cytometry. Then we co-cultured CD8 +TRM with endometrial stromal cells (ESCs) to explore the influence of CD8 +TRM on the expression of endometrial receptivity markers in ESCs. Next, we established mice models to find the number and function of CD8 +TRM and find immune intervention target of it <em>in vivo</em>. We found that the cytotoxicity, degranulation, and inflammatory cytokine expressed by CD8 +TRM were higher in endometriosis patients than in controls during secretory phase. CD8 +TRM inhibited the expression of HOXA10, FOXO1, IGFBP-1 and PRL of ESCs in a co-culture system. The expression of HOXA10, FOXO1, IGFBP-1 and PRL of ESCs were improved when blocking IFN-γ. And the number of CD8 +TRM and its cytotoxicity and expression of inflammatory cytokine were higher in endometriosis mice models than sham operation controls. After intraperitoneal injected anti-IFN-γ or anti-CD8α, the expression of endometrial receptivity makers was increased. These findings demonstrated that CD8 +TRM participate in the pro-inflammatory endometrial immune microenvironment and affect the expression of endometrial receptivity and decidualization markers in the eutopic endometrium of endometriosis patients and endometriosis mice models. CD8 +TRM and its IFN-γ secretion could be probably immunotherapeutic target for defective endometrial receptivity of endometriosis.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"Article 104737"},"PeriodicalIF":2.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferroptosis, NF-κB expression, and antioxidant imbalance in chorioamnionitis-associated preterm birth","authors":"Zhonglian Li ,&nbsp;Zixia Zhang ,&nbsp;Ruishi Luo ,&nbsp;Ping Jiang ,&nbsp;Mengjuan Qi ,&nbsp;Ying Guo","doi":"10.1016/j.jri.2025.104735","DOIUrl":"10.1016/j.jri.2025.104735","url":null,"abstract":"<div><div>Ferroptosis is a regulated form of cell death driven by iron-dependent lipid peroxidation and characterized by antioxidant imbalance, including reduced glutathione peroxidase 4 (GPX4) and an impaired nuclear factor erythroid 2–related factor 2 (NRF2)–heme oxygenase-1 (HO-1) axis. Infection-induced chorioamnionitis is a major risk factor for preterm birth (PTB). Emerging evidence indicates that infections can trigger ferroptosis, and nuclear factor kappa B (NF-κB) signaling may be involved. In this study, flow cytometry quantified plasma cytokines (IL-6, IL-1β, TNF-α) in normal controls (NC), non-chorioamnionitis-associated preterm birth (NC-PTB), and chorioamnionitis-associated preterm birth (CA-PTB) groups. ELISA measured malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and superoxide dismutase (SOD) in placental tissues; total iron (reported as Fe²⁺ equivalents) was quantified with a ferrozine-based assay after reducing ferric iron (Fe³⁺) to ferrous iron (Fe²⁺). Immunohistochemistry assessed NF-κB, GPX4, NRF2, and HO-1 in fetal membranes. The CA-PTB group showed higher inflammatory cytokines (IL-6, IL-1β, TNF-α) and NF-κB expression, alongside reduced antioxidant defenses (GPX4, NRF2, HO-1, SOD). Lipid peroxidation products (MDA, 4-HNE) and iron load (Fe²⁺) were also increased, indicating ferroptosis-related features. NF-κB correlated positively with inflammatory cytokines, lipid peroxidation products (MDA, 4-HNE) and iron load (Fe²⁺), and negatively with antioxidant indices (NRF2,SOD,HO-1); GPX4 showed no significant correlation with NF-κB. Antioxidant indices were inversely related to lipid peroxidation products and iron load. Taken together, our data indicate elevated ferroptosis-related features in CA-PTB alongside NF-κB expression and reduced antioxidant defenses. Whether infection-related NF-κB contributes to ferroptosis requires mechanistic confirmation.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"Article 104735"},"PeriodicalIF":2.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese medicines formula supplementation to late pregnancy sows enhance the ileal barrier and anti-inflammatory capacity of their offspring","authors":"Mingzheng Han,&nbsp;Quanwei Li,&nbsp;Jichang Deng,&nbsp;Na Qiao,&nbsp;Hui Zhang,&nbsp;Qingyue Han,&nbsp;Jianying Guo,&nbsp;Ying Li,&nbsp;Zhaoxin Tang","doi":"10.1016/j.jri.2025.104655","DOIUrl":"10.1016/j.jri.2025.104655","url":null,"abstract":"<div><h3>Background</h3><div>The nutritional status during the late stage of pregnancy significantly influences the survival rate, birth weight, growth performance, and immune function of piglets while also playing a pivotal role in regulating intestinal barrier function. However, it remains unclear whether supplementation with traditional Chinese medicine (TCM) can enhance offspring piglets' intestinal barrier function and immune function.</div></div><div><h3>Methods</h3><div>24 late-pregnancy sows (85 days pregnant) were randomly assigned to either CON group or TCM group for a 30-day feeding trial. Following parturition, 10 newborn piglets from each group were randomly selected for euthanasia, and the ileal tissues as well as cecal contents were collected for analysis. The hub active ingredients and targets of TCMs formula were analyzed using network pharmacology.</div></div><div><h3>Results</h3><div>TCM group exhibited elongation and deepening of villi in the ileum, a significant increase in Peyer's patches, notable enhancement of intestinal immune function, and improved anti-inflammatory ability. Further, maternal TCMs supplementation might increase the abundances of <em>Sutterella</em> and <em>Actinoplanes</em> in the piglet cecum, which significantly affected chemoheterotrophy, aerobic-chemoheterotrophy, and fermentation functions. PI3KCA and PI3KCB may serve as hub genes for the 14 TCMs. Kaempferol, Quercetin, and Luteolin were identified as the top three active ingredients with potential positive effects on Neonatal piglets through modulation of MAPK signaling pathway, calcium signaling pathway, and cAMP signaling pathway.</div></div><div><h3>Conclusion</h3><div>Incorporating Chinese herbs into the diets of sows significantly improves the Ileum barrier function and anti-inflammatory capacity of piglets, potentially attributable to active ingredients, modulation of intestinal microbial communities, and activation of diverse signaling pathways.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"Article 104655"},"PeriodicalIF":2.9,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic VSL#3 alleviates intrahepatic cholestasis of pregnancy by upregulating farnesoid X receptor-fibroblast growth factor 15 through regulation of the gut microbiota","authors":"Zhihua Zeng ,&nbsp;Hua Li ,&nbsp;Weitao Yang,&nbsp;Li Li,&nbsp;Ping Ni,&nbsp;Qiuling Chen,&nbsp;Wenjuan Zhou,&nbsp;Jing Peng,&nbsp;Le Huang","doi":"10.1016/j.jri.2025.104653","DOIUrl":"10.1016/j.jri.2025.104653","url":null,"abstract":"<div><div>Intrahepatic cholestasis of pregnancy (ICP) poses significant risks to both maternal and fetal health, and treatment options remain limited. This study investigated the efficacy and underlying mechanisms of VSL#3 in alleviating ICP. Clinical fecal and blood samples were collected from 26 patients with ICP and 21 healthy pregnant women. The gut microbiota composition was analyzed using 16S rRNA sequencing. To further explore causality, we established a fecal microbiota transplantation-ICP mouse model using fecal samples from ICP patients, as well as an estrogen-induced ICP mouse model. Compared with healthy pregnant women, ICP patients exhibited a distinct gut microbiota profile, characterized by an increased abundance of <em>Bacteroides</em> and <em>Alistipes</em>. Serum FGF19 levels were significantly lower in ICP patients, showing a negative correlation with liver function markers, such as serum total bile acid (TBA), and a positive correlation with beneficial genera including <em>Bifidobacterium</em>, <em>Ruminococcus</em>, <em>Blautia</em>, <em>Dorea, Eubacterium</em> (<em>hallii group</em>) and <em>Ruminococcus</em> (<em>torques group</em>). VSL#3 treatment in mice alleviated ICP manifestations by improving liver histopathology, reducing TBA and alanine aminotransferase levels, increasing FGF15 concentrations, and enhancing fetal outcomes. These beneficial effects were abolished by co-administration of the FXR antagonist Z-guggulsterone, confirming the role of FXR signaling. In conclusion, VSL#3 alleviated ICP by modulating the gut microbiota to activate the FXR-FGF15 axis, thereby reducing bile acid synthesis and improving maternal and fetal outcomes.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"Article 104653"},"PeriodicalIF":2.9,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Decreased membrane-bound suppressor of tumorigenicity 2 (ST2) but not soluble ST2 was associated with women with recurrent pregnancy loss and recurrent implantation failure compared to controls” [J. Reprod. Immunol. 170 (2025) 104619]","authors":"Thanh Vinh Luu ,&nbsp;Amy Thees ,&nbsp;Umida Ganieva ,&nbsp;Svetlana Dambaeva ,&nbsp;Joanne Kwak-Kim","doi":"10.1016/j.jri.2025.104650","DOIUrl":"10.1016/j.jri.2025.104650","url":null,"abstract":"","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"Article 104650"},"PeriodicalIF":2.9,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hidden diversity: Identification and characterisation of compartment-specific testicular macrophage populations","authors":"Sneha Biniwale ,&nbsp;Rukmali Wijayarathna ,&nbsp;Rosemary Genovese ,&nbsp;Christiane Pleuger ,&nbsp;Vishnu Kumar ,&nbsp;Vijay Singh ,&nbsp;Sudhanshu Bhushan ,&nbsp;Kate L. Loveland ,&nbsp;Andreas Meinhardt ,&nbsp;Mark P. Hedger","doi":"10.1016/j.jri.2025.104651","DOIUrl":"10.1016/j.jri.2025.104651","url":null,"abstract":"<div><div>Two major populations of resident macrophages have been identified in the mouse testis: interstitial tissue macrophages, characterised by high expression of CD206 and low or undetectable MHC class II antigen (MHCII) expression, and peritubular macrophages that generally lack CD206, but exhibit elevated MHCII. A morphometric analysis of macrophage subset marker antigens throughout the entire adult mouse testis was undertaken employing transgenic expression of the green fluorescent protein reporter at the locus of the <em>Cx3cr1</em> gene (<em>Cx3cr1</em>^{<em>gfp</em>/+}), and immunofluorescence localisation of F4/80, MHCII (I-A/I-E) and CD206. Compared with the testicular parenchyma encompassing the seminiferous tubules, the volume density of total macrophages (F4/80⁺) was 8–9-fold higher in the interstitial and peri-epithelial regions of the rete testis. Moreover, the majority of the interstitial and peri-epithelial macrophages in the rete testis were positive for both CD206 and MHCII – these double-positive macrophages were also numerous within and beneath the testis capsule. There was a small, but significant, increase (5–10 %) in the volume density of interstitial macrophages in the rete testis of mice with a heterozygous deletion of the <em>Inhba</em> (activin A subunit) gene. Macrophages increased considerably in the rete testis and adjacent testicular regions during infection with uropathogenic <em>Escherichia coli</em>. These data identify a substantial subset of macrophages within the mouse rete testis and subcapsular region that express both CD206 and MHCII, which comprises only a minor subset in the remainder of the testis. These double-positive macrophages may play a dual role in regulating tolerance to spermatozoa and responding to ascending infections.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"Article 104651"},"PeriodicalIF":2.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A role for TLR7 activation by miR-146a-3p in antiphospholipid antibody-induced trophoblast IL-8 and inflammasome-mediated IL-1β production","authors":"Lily C. Salmeron ,&nbsp;Tiffany N. Hidalgo ,&nbsp;Tatyana Lynn ,&nbsp;Deidre M. Jones ,&nbsp;Lawrence W. Chamley ,&nbsp;Vikki M. Abrahams","doi":"10.1016/j.jri.2025.104652","DOIUrl":"10.1016/j.jri.2025.104652","url":null,"abstract":"<div><div>Women with antiphospholipid antibodies (aPL) are at high risk for pregnancy complications. In obstetric antiphospholipid syndrome (APS), aPL specific for beta₂ glycoprotein I (β₂GPI), target the placental trophoblast leading to inflammation. Studies from our group have shown that aPL trigger trophoblast cells to produce elevated inflammatory IL-1β and IL-8 via activation of Toll-like receptor (TLR) 4, and that downstream, IL-1β is mediated by uric acid-induced NLRP3 inflammasome activation, and IL-8 is mediated by a novel TLR8-activating microRNA (miR), miR-146a-3p. The objective of this study was to further explore the role of TLR7- and TLR8-activating miRs in the aPL-driven trophoblast chemokine and inflammasome response. Using the human first trimester trophoblast cell line, Sw.71, we found that aPL elevated miR-146a-3p expression but had no effect on levels of miR-21a, miR-29a or Let-7b. aPL-induced trophoblast IL-8 secretion was reduced by the TLR7 inhibitor, IRS661, and by the TLR8 inhibitor, CU-CPT9a, while aPL-induced uric acid, caspase-1 activity, and IL-1β secretion was reduced only by the TLR7 inhibitor. Over expression of miR-146a-3p using a specific miR mimic induced trophoblast IL-8 secretion in a TLR7- and TLR8-dependent manner, while miR-146a-3p induced trophoblast IL-1β secretion only via TLR7. This study highlights a role for TLR7/TLR8-activating miR-146a-3p as an intermediate signal that contributes to and modulates upstream TLR-driven trophoblast inflammatory responses, and adds to our knowledge of the molecular and innate immune mechanisms that underpin the pathogenesis of obstetric APS.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"Article 104652"},"PeriodicalIF":2.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of platelet-rich plasma (PRP) on ovarian function and oocyte quality: A comprehensive review","authors":"Farahnazsadat Ahmadi ,&nbsp;Roghaye Gharaei ,&nbsp;Elham Shirali ,&nbsp;Khadijeh Adabi ,&nbsp;Valiollah Alishahi ,&nbsp;Mahboubeh Tajaldini","doi":"10.1016/j.jri.2025.104649","DOIUrl":"10.1016/j.jri.2025.104649","url":null,"abstract":"<div><div>Ovarian aging, premature ovarian insufficiency (POI), and decreased ovarian reserve (DOR) are major challenges in reproductive medicine, and few treatments are available to restore ovarian function reversibly. Platelet-rich plasma (PRP), an autologous concentrate rich in bioactive growth factors, has been proposed as a regenerative therapy for reversing ovarian aging. Recent evidence suggests that PRP enhances the ovarian endocrine function and oocyte competence by stimulating major survival signals, reducing oxidative stress, and promoting mitochondrial biogenesis. Preliminary clinical evidence and case reports show the potential for successful reproduction following intraovarian PRP administration. By critically evaluating existing clinical evidence and discussing the molecular mechanisms involved, this article highlights the potential PRP to act as a minimally invasive, autologous, and novel fertility regenerative strategy. It also emphasized the implementation of standardized protocols and robust clinical trials to establish the effectiveness of PRP in reproductive medicine. Therefore, this review aims to critically evaluate existing literature on the effects reported to date, while highlighting the very-low-certainty evidence base and the imperative for large, sham-controlled randomized trials to confirm efficacy and long-term safety.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"172 ","pages":"Article 104649"},"PeriodicalIF":2.9,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}