Journal of Reproductive Immunology最新文献

{"title":"Analysis of the presence of natural killer cell subpopulations in preterm human milk: A first approach","authors":"Mario Daniel Caba-Flores ,&nbsp;María de la Soledad Lagunes-Castro ,&nbsp;Aracely López-Monteon ,&nbsp;Rubí Viveros-Contreras ,&nbsp;Juan Gerardo Neme Kuri ,&nbsp;David Huerta-Morales ,&nbsp;Samantha Ponce Ramos ,&nbsp;Edith Nava Bustos ,&nbsp;Angel Ramos-Ligonio","doi":"10.1016/j.jri.2024.104394","DOIUrl":"10.1016/j.jri.2024.104394","url":null,"abstract":"<div><div>Several immune cell populations are transferred to the newborn through breast milk, including natural killer (NK) cells, which are critical for innate defense and regulation of the immune response, especially in preterm infants. The aim of this study was to analyze the presence of NK cell subpopulations in different types of preterm breast milk. The study quantified the presence of NK cell subpopulations by flow cytometry using the relative expression of CD56 and CD16 markers in colostrum, transitional and mature milk samples from preterm mothers. Flow cytometry analysis revealed the presence of five NK cell subpopulations, but unlike those reported in peripheral blood, CD56^dimCD16⁺ and CD56^-CD16⁺ populations are predominantly present in preterm milk, only the CD56^brightCD16^dim population is increased in mature milk. Analysis of NK cell subpopulations in preterm milk revealed a pattern of NK cell presence in preterm breast milk with predominantly cytotoxic phenotypes in relation to CD16 marker expression.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104394"},"PeriodicalIF":2.9,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CX3CL1 (Fractalkine): An important cytokine in physiological and pathological pregnancies","authors":"Xianyang Hu ,&nbsp;Xixi Huang ,&nbsp;Tingxuan Yin ,&nbsp;Jiajia Chen ,&nbsp;Weijie Zhao ,&nbsp;Min Yu ,&nbsp;Lu Liu ,&nbsp;Meirong Du","doi":"10.1016/j.jri.2024.104392","DOIUrl":"10.1016/j.jri.2024.104392","url":null,"abstract":"<div><div>C-X3-C motif chemokine ligand 1 (CX3CL1), commonly known as Fractalkine, is an important chemokine with dual functions of chemotaxis and adhesion. It plays a pivotal role in a variety of physiological processes and pathological conditions, particularly in conjunction with its receptor, C-X3-C motif chemokine receptor 1 (CX3CR1). This review focuses on the expression and intricate regulatory mechanisms of CX3CL1 at the maternal-fetal interface, emphasizing its multifaceted role during pregnancy. CX3CL1 was detected in the trophoblast and decidua tissues, playing a crucial role in recruitment of immune cells, enhancing endometrial receptivity, and modulating trophoblast cell activities. Abnormal expression of CX3CL1 has been correlated with adverse pregnancy outcomes such as spontaneous abortion, gestational diabetes, preeclampsia, and preterm births. By elucidating the complex interplay of CX3CL1 at the maternal-fetal interface, this review aims to shed light on its potential roles in pregnancy-related complications.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104392"},"PeriodicalIF":2.9,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between cellular immune and preeclampsia and preterm birth: A Mendelian randomization study","authors":"Runfang Wang,&nbsp;Cuilian Liu,&nbsp;Xiaodan Liu,&nbsp;Li Liu,&nbsp;Yuange Xiao,&nbsp;Yan Huo","doi":"10.1016/j.jri.2024.104391","DOIUrl":"10.1016/j.jri.2024.104391","url":null,"abstract":"<div><div>Emerging evidences have highlighted immune-inflammatory imbalances as a critical driver of the pathogenesis for preeclampsia (PE) and preterm birth (PB), but the impact of specific immune factors on the diseases is largely unknown. Our aim was to determine whether these immune cells are causally associated with the onset of PE or PB. Drawing on publicly available genetic data, we applied Mendelian randomization analysis to probe the causal link of 731 immune traits (7 panels: TBNK panel, Regulatory T cells panel, Maturation stages of T-cell panel, Dendritic cell panel, B-cell panel, Monocyte panel and Myeloid cell pane) with the risk of PE and PB. The inverse variance weighting method (IVW) acted as the primary analysis to estimate the validity of causality, and sensitivity analyses were conducted to assessment of heterogeneity and pleiotropy. After adjusting for P-values for FDR method, CD27 on CD24+ CD27+ B cell, CD80 on plasmacytoid Dendritic Cell, CD33+ HLA DR+ CD14dim Absolute Count, CD33+ HLA DR+ CD14- Absolute Count, CD33+ HLA DR+ Absolute Count were remarkably causally involved in increased risk of PE, while HLA DR on Dendritic Cell exerted a protective causation against PE (P_FDR &lt; 0.05). Moreover, we determined that CD45 on CD33dim HLA DR- in myeloid cells decreased PB risk, whereas CD11b on Granulocytic Myeloid-Derived Suppressor Cells had the opposite effect on PB (F_FDR &lt; 0.2). This study provided genetic evidence for causal relationships between immune cell traits and PE and PB, offering potential candidate immunophenotypes for future studies on the etiology of pregnancy complications.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104391"},"PeriodicalIF":2.9,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estriol in formation of mononuclear cells tolerogenic features","authors":"Irina Nekrasova,&nbsp;Sergei Shirshev","doi":"10.1016/j.jri.2024.104390","DOIUrl":"10.1016/j.jri.2024.104390","url":null,"abstract":"<div><div>Estriol (E₃) is one of hormones whose synthesis is mainly associated with pregnancy. The hormone can also regulate immune cells functions. E₃ influence on monocyte indoleamine-2,3-dioxygenase (IDO1) activity and Treg and NK cells’ markers expression was investigated. LPS and IFN-γ stimulated IDO1 activity was augmented by Е₃ independently of the hormone doses. Both Е₃ concentrations increased CD4⁺Foxp3⁺ lymphocytes percentage. This hormonal effect was mediated by protein kinase A. CD16 expression upon NK cells was reduced as a result of Е₃ influence. The data indicated that Е₃ is one of the factors which maintained immune tolerance during pregnancy and protected fetus preservation.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104390"},"PeriodicalIF":2.9,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics analysis of differential gene expression and immune and inflammatory response mechanisms in patients with typical and non-criteria obstetric antiphospholipid syndrome (OAPS and NC-OAPS)","authors":"Xuan Qi ,&nbsp;Peng Liu ,&nbsp;Yingjie Zhou ,&nbsp;Lingyan Lei ,&nbsp;Guoyu Xue ,&nbsp;Ronghua Wang ,&nbsp;Junping Wang ,&nbsp;Huifang Guo","doi":"10.1016/j.jri.2024.104389","DOIUrl":"10.1016/j.jri.2024.104389","url":null,"abstract":"<div><div>In this study, we investigated the molecular differences between patients with typical obstetric antiphospholipid syndrome (OAPS) and patients with non-criteria obstetric antiphospholipid syndrome (NC-OAPS) patients through transcriptome sequencing of peripheral blood samples from ten OAPS patients and ten NC-OAPS patients. Differentially expressed genes (DEGs) were identified, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, protein-protein interaction (PPI) analysis, and competitive endogenous RNA (ceRNA) network construction to identify hub genes. Verification was performed via Quantitative Real-time PCR (qPCR) in OAPS (n=9) and NC-OAPS (n=12) samples. We identified 240 DEGs in two groups. GO and KEGG analyses reviewed upregulated in pathways related to the inflammatory response; immune response; antigen processing and presentation; Th1, Th2, and Th17 cell differentiation; and NK cell-mediated cytotoxicity in OAPS patients. PPI and ceRNA network analyses identified key genes, with significant upregulation of CXCR2, JAK2, and MPO found in the OAPS group, which correlated with severe inflammation, JAK-STAT pathway activation, and increased NET activity in neutrophils. Other genes such as CD4, IL2RB, and NKG7, are involved in T-cell and NK cell regulation. Our results indicate enhanced inflammatory and immune responses in OAPS patients, suggesting more severe immune activity than in NC-OAPS patients, providing a basis for precise diagnostic and therapeutic strategies.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104389"},"PeriodicalIF":2.9,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased circulating levels of novel anti-inflammatory cytokines IL-27 and IL-38 are associated with immunoendrocrine dysregulation and altered redox stress in polycystic ovarian syndrome","authors":"Sugumar Shruthi ,&nbsp;Veerasamy Nirmaladevi ,&nbsp;Vivekanandhan Aravindhan","doi":"10.1016/j.jri.2024.104388","DOIUrl":"10.1016/j.jri.2024.104388","url":null,"abstract":"<div><h3>Background</h3><div>Polycystic ovary syndrome (PCOS) is a common metabolic/ endocrine disorder seen predominantly in women in their reproductive age, which increases the risk of infertility, endometrial cancer and metabolic disorders. IL-27 and IL-38 are recently discovered, novel anti-inflammatory cytokines whose role in immune-endocrine dysfunction seen in PCOS is largely unknown.</div></div><div><h3>Methods</h3><div>In the present study, we quantified these two cytokines along with markers for meta-inflammation (TNF-α, IL-6, IL-1β, IL-1Ra, IL-10 and TGF-β) and hormonal dysregulation (insulin, leptin, adiponectin, FGF-21, testosterone and DHEA-S) in the serum of PCOS women (n=44), along with age matched controls (n=20), by ELISA. We quantified serum lipid peroxidation, protein peroxidation, and nitrite levels using spectrophotometry.</div></div><div><h3>Results</h3><div>PCOS women had significantly elevated levels of IL-27, IL-38 along with TNF-α, IL-6, IL-1Ra, IL-10, FGF-21 and adiponectin, and decreased levels of TGF-β, SDF-1 and leptin. While there is no significant difference with respect to redox markers, nitrite levels were significantly increased in PCOS cases.</div></div><div><h3>Conclusion</h3><div>The increased circulating levels of anti-inflammatory cytokines IL-27 and IL-38 under PCOS conditions warrant further investigation. To the best of our knowledge, this is the first report on IL-38 levels in PCOS.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104388"},"PeriodicalIF":2.9,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oocyte donation pregnancies with high fetal-maternal immunogenetic dissimilarity show alterations in the maternal peripheral immunoregulatory response","authors":"K. van Bentem ,&nbsp;L.J. Verleng ,&nbsp;G.L. Lafeber ,&nbsp;X. Tian ,&nbsp;E. van Beelen ,&nbsp;C. van der Keur ,&nbsp;J.M. Kapsenberg ,&nbsp;E.E.L.O. Lashley ,&nbsp;M. Eikmans ,&nbsp;M.L.P. van der Hoorn","doi":"10.1016/j.jri.2024.104387","DOIUrl":"10.1016/j.jri.2024.104387","url":null,"abstract":"<div><div>Oocyte donation (OD) pregnancies result in increased fetal-maternal immunogenetic dissimilarity due to paternal and donor-derived genes. Higher fetal-maternal HLA mismatches are correlated with preeclampsia. Therefore, this study explored the maternal immune response, focusing on regulatory T cells (Tregs) during low versus high allogeneic pregnancies, and healthy versus preeclamptic OD pregnancies. Ten healthy and five preeclamptic OD pregnancies were included. Maternal peripheral blood was collected at different stages of pregnancy. Fetal-maternal HLA mismatches were determined, and immunophenotyping of peripheral blood mononuclear cells was conducted using a 22-colour spectral flow cytometry panel. Cytokines and hormones were detected in maternal plasma using ELISA and Luminex assays. The findings show similarities, but also distinct differences between low and high allogeneic healthy OD pregnancies. Early high allogeneic OD pregnancy showed reduction in Tregs, and CD8+ T cells, alongside lower percentage of effector/memory Tregs expressing PD-1 and Helios. Additionally, high allogeneic OD pregnancies showed increased IL-6 and progesterone in the first trimester. These variations suggest a different mode of immune regulation in early high allogeneic OD pregnancies, possibly to maintain healthy pregnancy. Further comparative analyses revealed reduced CD45RO+CTLA-4+ Tregs and increased latent TGF-β1 and -β2 levels in early preeclamptic compared to healthy OD pregnancy. Late-stage preeclamptic OD pregnancies exhibited higher frequencies of CD45RO+TIGIT+ Tregs and higher levels of TNFα, indicating both a regulatory and pro-inflammatory environment. Overall, this study sheds light on the course of various immunoregulatory key players in OD pregnancy, and expands knowledge on maternal tolerance in this particular type of pregnancy.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104387"},"PeriodicalIF":2.9,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role and potential treatment of macrophages in patients with infertility and endometriosis","authors":"Linlin Song ,&nbsp;Caihong Yang ,&nbsp;Guiyi Ji ,&nbsp;Rong Hu","doi":"10.1016/j.jri.2024.104384","DOIUrl":"10.1016/j.jri.2024.104384","url":null,"abstract":"<div><div>Endometriosis is characterized as a macrophage-related ailment due to its strong link with immune dysfunction. Understanding the status of macrophage polarization in the context of endometriosis-related infertility is crucial for advancing diagnostic and therapeutic strategies. Our comprehensive review delves into the foundational understanding of macrophages and their profound influence on both endometriosis and infertility. Additionally, we illuminate the complex role of macrophages in infertility and endometriosis specifically. Finally, we focused on four critical dimensions: follicular fluid, the intraperitoneal environment, endometrial receptivity, and strategies for managing endometriosis. It is clear that throughout the progression of endometriosis, the diverse polarization states of macrophages play a pivotal role in the internal reproductive environment of infertile individuals grappling with this condition. Modulating macrophage polarization in the reproductive environment of endometriosis patients could address infertility challenges more effectively, offering a promising pathway for treating infertility associated with endometriosis.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104384"},"PeriodicalIF":2.9,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reply to ‘‘The Association of Systemic Immune-Inflammation Index (SII), Systemic Immune-Response Index (SIRI), and Neutrophil-to-Lymphocyte Ratio (NLR) with Cesarean Scar Pregnancy (CSP)’’","authors":"Refaettin Sahin,&nbsp;Atakan Tanacan,&nbsp;Dilek Sahin","doi":"10.1016/j.jri.2024.104386","DOIUrl":"10.1016/j.jri.2024.104386","url":null,"abstract":"<div><div>We have read the correspondance written by Jin et al. with great interest. It is an honor for us to be followed by high quality academicians around the world. We want to thank the authors for their interest on our manuscript and in our opinion the manusript will be much more improved after our reply to the concerns underlined by Jin et al.All of the blood samples were collected before the administration of MTX in the present study. Cases with chronic diseases or infections that may affect the complete blood cell indices were excluded from the study. Thus, complete blood cell indices evaluated in the present study were not affected by the mentioned factors.We hope our reply will be sufficient to relieve the concerns of the readers. Thank you very much for your interest.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104386"},"PeriodicalIF":2.9,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HLA-G - evolvement from a trophoblast specific marker to a checkpoint molecule in cancer, a narrative review about the specific role in breast- and gynecological cancer","authors":"Julia Knabl ,&nbsp;Yao Ye ,&nbsp;Gernot Desoye ,&nbsp;Udo Jeschke","doi":"10.1016/j.jri.2024.104385","DOIUrl":"10.1016/j.jri.2024.104385","url":null,"abstract":"<div><div>Human leukocyte antigen G (HLA-G) is known as a non-classical molecule of the major histocompatibility complex class Ib and downregulates the mother's immune response against the fetus during pregnancy, thereby generating immune tolerance. Due to the latter effect, HLA-G is also referred to as an immune checkpoint molecule. Originally identified on extravillous trophoblasts, HLA-G is already known to induce immune tolerance at various stages of the immune response, for example through cell differentiation and proliferation, cytolysis and cytokine secretion. Because of these functions, HLA-G is involved in various processes of cancer progression, but a comprehensive review of the role of HLA-G in gynecologic cancers is lacking. Therefore, this review focuses on the existing knowledge of HLA-G in ovarian cancer, endometrial cancer, cervical cancer and breast cancer. HLA-G is predominantly expressed in cancer tissues adjacent to the extravillous trophoblast. Therefore, modulating its expression in the cancer target tissues of cancer patients could be a potential therapeutic approach to treat these diseases.</div></div>","PeriodicalId":16963,"journal":{"name":"Journal of Reproductive Immunology","volume":"166 ","pages":"Article 104385"},"PeriodicalIF":2.9,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}