Journal of Reproduction and Development最新文献_第9页

Parallel expression patterns of NR4A nuclear receptor family genes in the pituitary gland of proestrus rats. 发情大鼠垂体中 NR4A 核受体家族基因的平行表达模式

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-04-04 Epub Date: 2024-02-09 DOI: 10.1262/jrd.2023-090

Ryota Terashima, Daiki Nagao, Masato Ikeo, Keisuke Morioka, Titaree Laoharatchatathanin, Shiro Kurusu, Mitsumori Kawaminami

{"title":"Parallel expression patterns of NR4A nuclear receptor family genes in the pituitary gland of proestrus rats.","authors":"Ryota Terashima, Daiki Nagao, Masato Ikeo, Keisuke Morioka, Titaree Laoharatchatathanin, Shiro Kurusu, Mitsumori Kawaminami","doi":"10.1262/jrd.2023-090","DOIUrl":"10.1262/jrd.2023-090","url":null,"abstract":"The NR4A nuclear receptor family (NR4As), encompassing NR4A1, NR4A2, and NR4A3, exerts pivotal roles in cellular processes through intricate expression patterns and interactions. Despite the influence of some NR4As on anterior pituitary functions regulated by the hypothalamus, their physiological expression patterns remain unclear. In our prior work, we demonstrated the specific upregulation of NR4A3 in the rat anterior pituitary gland during the proestrus afternoon, coinciding with a gonadotropin surge. In this study, we investigated changes in pituitary Nr4a gene expression throughout the estrous cycle in rats and a gonadotropin surge-induced model. Nr4a1 and Nr4a2 gene expression significantly increased during proestrus, aligning with previous observations for Nr4a3. Furthermore, prolactin gene expression increased sequentially with rising Nr4a gene expression, while thyroid-stimulating hormone beta gene expression remained stable. Immunohistochemistry revealed a widespread and differential distribution of NR4A proteins in the anterior pituitary, with NR4A1 and NR4A3 being particularly abundant in thyrotrophs, and NR4A2 in gonadotrophs. In estrogen-treated ovariectomized rats, elevated luteinizing hormone secretion corresponded to markedly upregulated expression of Nr4a1, Nr4a2, and Nr4a3. In gonadotroph and somatomammotroph cell lines, gonadotropin- and thyrotropin-releasing hormones transiently and dose-dependently increased the expression of Nr4a genes. These findings suggest that hypothalamic hormone secretion during proestrus may induce the parallel expression of pituitary Nr4a genes, potentially influencing the pituitary gene expression program related to endocrine functions before and after ovulation.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"115-122"},"PeriodicalIF":1.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139723086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of globin peptide on female fertility in aging granulosa cell-specific Nrg1 knockout mice 球蛋白肽对衰老颗粒细胞特异性 Nrg1 基因敲除小鼠雌性生育能力的影响

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-03-12 DOI: 10.1262/jrd.2023-076

Takashi UMEHARA, Marino OGASAHARA, D.M.V. Supun PREMARATHNE, Yuka SASAKAWA, Yasuo SUMIDA, Masayuki SHIMADA

{"title":"Effect of globin peptide on female fertility in aging granulosa cell-specific Nrg1 knockout mice","authors":"Takashi UMEHARA, Marino OGASAHARA, D.M.V. Supun PREMARATHNE, Yuka SASAKAWA, Yasuo SUMIDA, Masayuki SHIMADA","doi":"10.1262/jrd.2023-076","DOIUrl":"https://doi.org/10.1262/jrd.2023-076","url":null,"abstract":"Ovarian fibrosis contributes to age-related ovarian dysfunction. In our previous study, we observed ovarian fibrosis in both obese and aging mice with intracellular lipid droplets in the fibrotic ovaries. Although the importance of mitochondria in ovarian fibrosis has been recognized in pharmacological studies, their role in lipid metabolism remains unclear. Globin peptide (GP), derived from hemoglobin, enhances lipid metabolism in obese mice. This study aimed to elucidate the importance of lipid metabolism in ovarian fibrosis by using GP. Treatment of ovarian stromal cells with GP increased mitochondrial oxygen consumption during β-oxidation. Lipid accumulation was also observed in the ovaries of granulosa cell-specific Nrg1 knockout mice (gcNrg1KO), and the administration of GP to gcNrg1KO mice for two months reduced ovarian lipid accumulation and fibrosis in addition to restoring the estrous cycle. GP holds promise for mitigating lipid-related ovarian issues and provides a novel approach to safeguarding ovarian health by regulating fibrosis via lipid pathways.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-076/figure/advpub_2023-076.jpg\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"285 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140105679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin structure in totipotent mouse early preimplantation embryos 全能小鼠植入前早期胚胎的染色质结构

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-03-08 DOI: 10.1262/jrd.2023-106

Masatoshi OOGA

{"title":"Chromatin structure in totipotent mouse early preimplantation embryos","authors":"Masatoshi OOGA","doi":"10.1262/jrd.2023-106","DOIUrl":"https://doi.org/10.1262/jrd.2023-106","url":null,"abstract":"Totipotency refers to the ability of a single cell to give rise to all the different cell types in the body. Terminally differentiated germ cells (spermatozoa and oocytes) undergo reprogramming, which results in the acquisition of totipotency in zygotes. Since the 1990s, numerous studies have focused on the mechanisms of totipotency. With the emergence of the concept of epigenetic reprogramming, which is important for the undifferentiated and differentiated states of cells, the epigenomes of germ cells and fertilized eggs have been thoroughly analyzed. However, in early immunostaining studies, detailed epigenomic information was difficult to obtain. In recent years, the explosive development of next-generation sequencing has made it possible to acquire genome-wide information and the rise of genome editing has facilitated the analysis of knockout mice, which was previously difficult. In addition, live imaging can effectively analyze zygotes and 2-cell embryos, for which the number of samples is limited, and provides biological insights that cannot be obtained by other methods. In this review, the progress of our research using these advanced techniques is traced back from the present to its earliest years.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-106/figure/advpub_2023-106.png\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140056941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm 高浓度牛血清白蛋白可提高冷藏大鼠精子的受精能力

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-03-01 DOI: 10.1262/jrd.2023-085

Katsuma YAMAGA, Satohiro NAKAO, Nobuyuki MIKODA, Jorge Mario SZTEIN, Naomi NAKAGATA, Toru TAKEO

{"title":"High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm","authors":"Katsuma YAMAGA, Satohiro NAKAO, Nobuyuki MIKODA, Jorge Mario SZTEIN, Naomi NAKAGATA, Toru TAKEO","doi":"10.1262/jrd.2023-085","DOIUrl":"https://doi.org/10.1262/jrd.2023-085","url":null,"abstract":"Cold transport of the cauda epididymides is a useful technique for shipping laboratory rat sperm. Cold transport of rat sperm avoids potential risks of microbiological infection, animal escape or death, and animal welfare issues. Previously, we reported that a cold-storage solution containing dimethyl sulfoxide and quercetin maintained the fertility of cold-stored rat sperm. However, cold-stored rat sperm exhibited a decreased fertilization rate after 24-h storage. To recover the fertility of cold-stored sperm, we focused on the effects of bovine serum albumin (BSA), a cholesterol acceptor that induces sperm capacitation. We sought to determine the optimal concentration of BSA in fertilization medium based on the fertility of cold-stored rat sperm. High concentrations of BSA (40 mg/ml) enhanced the fertilization rate of cold-stored rat sperm and maintained sperm fertility for 144 h. Embryos derived from cold-stored and BSA-treated sperm normally developed into pups after embryo transfer. In summary, high BSA concentrations enhanced the fertility of cold-stored rat sperm and prolonged the storage period to 144 h, thereby expanding the transportable region for genetically engineered rats.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-085/figure/advpub_2023-085.png\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"149 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140008653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes 排卵和授精之间的时间间隔决定大鼠受精卵细胞的质量

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-26 DOI: 10.1262/jrd.2023-067

Naomi NAKAGATA, Satohiro NAKAO, Nobuyuki MIKODA, Katsuma YAMAGA, Toru TAKEO

{"title":"Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes","authors":"Naomi NAKAGATA, Satohiro NAKAO, Nobuyuki MIKODA, Katsuma YAMAGA, Toru TAKEO","doi":"10.1262/jrd.2023-067","DOIUrl":"https://doi.org/10.1262/jrd.2023-067","url":null,"abstract":"Genetically modified rats are valuable models in human disease research. We recently developed an improved system for rat sperm cryopreservation and in vitro fertilization (IVF) that facilitates the efficient production and preservation of genetically modified rats. In the IVF procedure performed using frozen–thawed rat sperm, the IVF schedule is fixed to ensure timely hormone administration and oocyte collection. To enhance the flexibility of the IVF schedule, possible periods of postovulated rat oocytes with normal fertility and developmental abilities should be determined. Therefore, in this study, we examined the fertilization and developmental ability of incubated oocytes 1–13 h after oocyte collection at 9:00 AM. The fertilization rate decreased 7 h after oocyte collection, and abnormally fertilized oocytes appeared 10 h after oocyte collection. The developmental rate also decreased 7 h after oocyte collection; however, live pups were obtained from oocytes 12 h after oocyte collection. In summary, ovulated rat oocytes exhibited a high developmental ability after IVF for up to 4 h after oocyte collection.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-067/figure/advpub_2023-067.png\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"97 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139950578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of chemokines in regulating luteal and uterine functions in pregnant cows 趋化因子在调节妊娠母牛黄体和子宫功能中的作用

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-25 DOI: 10.1262/jrd.2023-100

Ryosuke SAKUMOTO

引用次数: 0

Capturing temperature changes on the ocular surface along with estrus and ovulation using infrared thermography in Japanese Black cows. 利用红外热像仪捕捉日本黑牛眼表温度随发情和排卵的变化。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-19 Epub Date: 2023-11-23 DOI: 10.1262/jrd.2022-116

Riho Ozaki, Seiji Inoue, Yuki Yorozui, Rei Ichikawa, Naoki Yamada, Seiya Higashi, Shuichi Matsuyama, Hiroko Tsukamura, Satoshi Ohkura, Yoshihisa Uenoyama, Yasuhiro Morita

{"title":"Capturing temperature changes on the ocular surface along with estrus and ovulation using infrared thermography in Japanese Black cows.","authors":"Riho Ozaki, Seiji Inoue, Yuki Yorozui, Rei Ichikawa, Naoki Yamada, Seiya Higashi, Shuichi Matsuyama, Hiroko Tsukamura, Satoshi Ohkura, Yoshihisa Uenoyama, Yasuhiro Morita","doi":"10.1262/jrd.2022-116","DOIUrl":"10.1262/jrd.2022-116","url":null,"abstract":"Pre-ovulatory follicles are cooler than the neighboring reproductive organs in cows. Thus, measuring the temperature of reproductive organs could be a useful method for predicting estrus and ovulation in cows, and the establishment of a non-invasive technique is required. In this study, we used infrared thermography (IRT) to measure ocular surface temperature as a potential surrogate for reproductive organ temperature. Five Japanese Black cows with synchronized estrus were subjected to temperature measurements in five regions of the ocular surface, including the nasal conjunctiva, nasal limbus, center cornea, temporal limbus, and temporal conjunctiva, twice a day (0800 h and 1600 h) during the experimental period. The temperatures in the five regions significantly declined in cows from estrus to ovulation. To the best of our knowledge, this study is the first to use IRT to show a temperature decrease in the ocular surface along with estrus to ovulation in Japanese Black cows.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"49-54"},"PeriodicalIF":1.8,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10902635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138440895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PABPN1L is required for maternal mRNA degradation after meiosis resumption. 减数分裂恢复后母体mRNA降解需要PABPN1L。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-19 Epub Date: 2023-12-07 DOI: 10.1262/jrd.2023-077

Chihiro Emori, Mayo Kodani, Ferheen Abbasi, Masashi Mori, Masahito Ikawa

引用次数: 0

Lipid droplets synthesized during luteinization are degraded after pregnancy 黄体化过程中合成的脂滴在怀孕后会降解

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-04 DOI: 10.1262/jrd.2023-095

Junichiro MITSUI, Megumi IBAYASHI, Ryutaro AIZAWA, Tomonori ISHIKAWA, Naoyuki MIYASAKA, Satoshi TSUKAMOTO

{"title":"Lipid droplets synthesized during luteinization are degraded after pregnancy","authors":"Junichiro MITSUI, Megumi IBAYASHI, Ryutaro AIZAWA, Tomonori ISHIKAWA, Naoyuki MIYASAKA, Satoshi TSUKAMOTO","doi":"10.1262/jrd.2023-095","DOIUrl":"https://doi.org/10.1262/jrd.2023-095","url":null,"abstract":"After pregnancy, the corpus luteum (CL) functions as a transient endocrine gland that produces progesterone, which is necessary to maintain pregnancy. To maintain constant progesterone production, CLs are enriched in lipids as its precursors. Lipid droplets (LDs) are organelles that originate from the endoplasmic reticulum and store neutral lipids such as triacylglycerols and cholesteryl esters. The size and number of LDs in a cell are regulated by LD-associated proteins that coat their surface. LD degradation is regulated by either neutral lipid hydrolases (lipolysis), selective autophagic mechanism (lipophagy), or both. Mammalian CLs are long known to be enriched in LDs, but LDs are rapidly depleted after pregnancy and reappear near the time of delivery. In this present study, we hypothesized that LDs synthesized by luteinization are massively degraded after pregnancy. Using mCherry-HPos mice, in which LD synthesis can be visualized in vivo, we found that LD synthesis, which was activated during luteal development, was suppressed after implantation. In CLs, LD synthesis remained low during pregnancy, but was reactivated before and after delivery. These changes in LDs were confirmed using electron microscopy and immunostaining. Furthermore, LD degradation was mediated by lipolysis rather than lipophagy. In summary, our findings indicate that luteinization-induced LD synthesis is suppressed after pregnancy onset and that CLs are lipid-poor during pregnancy because LDs stored during luteal development are extensively degraded by lipolysis.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-095/figure/advpub_2023-095.jpg\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"4 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139678088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The fertility of dairy heifers and cows is not influenced by the follicular wave of the ovulatory follicle 乳用小母牛和奶牛的繁殖力不受排卵卵泡的卵泡波影响

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-01-20 DOI: 10.1262/jrd.2023-084

Javad MOHAMMADI, Mehdi AZARI, Mojtaba KAFI

{"title":"The fertility of dairy heifers and cows is not influenced by the follicular wave of the ovulatory follicle","authors":"Javad MOHAMMADI, Mehdi AZARI, Mojtaba KAFI","doi":"10.1262/jrd.2023-084","DOIUrl":"https://doi.org/10.1262/jrd.2023-084","url":null,"abstract":"Two studies were conducted to evaluate the effects of the follicular wave on ovarian function and fertility in dairy heifers and lactating cows. In study 1, the estrous cycle of the selected Holstein heifers was initially synchronized using two intra-muscular prostaglandin F2α (PGF2α) administrations 11 days apart. Heifers in group FFW (n = 14) received an intra-muscular 500 μg PGF2α administration on day 7 after detecting standing estrus, while Heifers in group SFW (n = 14) were administered PGF2α 13 days after detecting standing estrus. The pregnancy rates of FFW (n = 98) and SFW (n = 100) heifers were also determined 35–37 days after artificial insemination (AI). In Study 2, healthy Holstein lactating cows (n = 28) were randomly assigned to either the FFW (n = 14) or SFW (n = 14) groups. The estrous cycles of the cows were presynchronized using two intra-muscular administrations of PGF2α given 14 days apart. Then, the emergences of the follicular waves were induced using an Ovsynch protocol. The pregnancy rate of FFW (n = 99) versus SFW (n = 98) cows was also determined 35–37 days after AI. The ovulatory follicle and corpus luteum (CL) resulting from the ovulatory follicle of FFW were larger than those of the dominant follicle and the CL of SFW in dairy heifers and lactating cows. However, the pregnancy rate did not differ between the FFW and SFW groups in heifers and lactating cows 35–37 days after AI. In conclusion, although the characteristics of the ovulatory follicles in FFW versus SFW animals differed, the follicular wave in dairy heifers or lactating cows did not affect fertility.\u0000","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"13 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139501064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0