Journal of Reproduction and Development最新文献_第9页

Mean distance from skin to epiglottis in parturients as measured by airway ultrasound. 通过气道超声波测量的产妇会厌到皮肤的平均距离。

IF 4.4 4区生物学

Journal of Reproduction and Development Pub Date : 2023-11-01 Epub Date: 2022-12-27 DOI: 10.1177/1742271X221140017

Emma Neish, Jeremy Collins, Roman M Sniecinski

{"title":"Mean distance from skin to epiglottis in parturients as measured by airway ultrasound.","authors":"Emma Neish, Jeremy Collins, Roman M Sniecinski","doi":"10.1177/1742271X221140017","DOIUrl":"10.1177/1742271X221140017","url":null,"abstract":"Introduction: There is an increasing interest in using airway ultrasound to predict difficult intubation. Studies to date have excluded pregnant women in reporting airway measurements. We performed this study to compare the mean distance from skin to epiglottis in parturients to that reported in previously published studies. We also assessed the correlation of mean distance from skin to epiglottis with other elements of the airway examination.Methods: A total of 100 parturients were recruited from a tertiary hospital's labor and delivery floor. Standard physical examination parameters were recorded in addition to the mean distance from skin to epiglottis for all subjects. The ratio of height-to-thyromental distance was used to classify airways as potentially favorable or unfavorable.Results: The average mean distance from skin to epiglottis in parturients was 19.9 ± 3.3 mm and followed a normal distribution. The mean distance from skin to epiglottis was moderately correlated with height and body mass index. There was no difference in mean distance from skin to epiglottis between subjects with favorable versus unfavorable airways as classified by ratio of height-to-thyromental distance.Conclusion: The typical mean distance from skin to epiglottis in parturients falls between previously published values in mixed populations. Previously published cut-off values using airway ultrasound to predict difficult intubation are not likely to apply to parturients.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"41 1","pages":"254-258"},"PeriodicalIF":4.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88256715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of rat spermatozoal hyperactivation by progesterone. 黄体酮对大鼠精子过度激活的增强作用。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-10-20 Epub Date: 2023-09-10 DOI: 10.1262/jrd.2023-040

Yuki Miyazawa, Masakatsu Fujinoki

引用次数: 0

Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period. 细胞外小泡在胚胎植入期建立过程中的整体分析和潜在影响。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-10-20 Epub Date: 2023-07-25 DOI: 10.1262/jrd.2023-044

Keigo Nakamura, Kazuya Kusama, Masatoshi Hori, Kazuhiko Imakawa

{"title":"Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period.","authors":"Keigo Nakamura, Kazuya Kusama, Masatoshi Hori, Kazuhiko Imakawa","doi":"10.1262/jrd.2023-044","DOIUrl":"10.1262/jrd.2023-044","url":null,"abstract":"Intrauterine extracellular vesicles (EVs) are involved in establishing proper conceptus-endometrial communication, which is essential for conceptus implantation and subsequent successful placentation. Despite several studies on intrauterine EVs, the composition and quantitative changes in conceptus and endometrial EVs, as well as the effects of intrauterine EVs on endometrial epithelial cells (EECs) during the peri-implantation period, have not been well characterized. To elucidate global changes in proteins in EVs extracted from uterine flushings (UFs) during the pre-implantation (P17), just-implantation (P20), and post-implantation (P22) periods, the datasets of the proteome iTRAQ analysis were compared among P17, P20, and P22 EVs. These analyses revealed that the composition and function of proteins in the EVs changed dramatically during peri-implantation in cattle. Notably, intrauterine P17 EVs affected the high expression of \"Developmental Biology\" and \"morphogenesis of an endothelium\" compared with those in P20 and P22 EVs. Furthermore, P20 EVs had the functions of the high expression of \"mitochondrial calcium ion homeostasis\" and \"Viral mRNA Translation\" compared with those in P17 EVs. Transcripts extracted from EECs treated with P17, P20, or P22 EVs were subjected to RNA-seq analysis. These analyses identified 60 transcripts in EECs commonly induced by intrauterine EVs recovered from P17, P20, and P22, a large number of which were associated with \"type I interferon signaling pathway\". Collectively, these findings reveal the presence and multiple functions of EVs that are potentially implicated in facilitating conceptus implantation into the uterine epithelium during the peri-implantation period.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"246-253"},"PeriodicalIF":1.8,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c8/de/jrd-69-246.PMC10602766.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9873723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex difference in developmental changes in visualized Kiss1 neurons in newly generated Kiss1-Cre rats. 新生Kiss1-Cre大鼠Kiss1神经元发育变化的性别差异。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-10-20 Epub Date: 2023-07-28 DOI: 10.1262/jrd.2023-019

Koki Yamada, Mayuko Nagae, Tetsuya Mano, Hitomi Tsuchida, Safiullah Hazim, Teppei Goto, Makoto Sanbo, Masumi Hirabayashi, Naoko Inoue, Yoshihisa Uenoyama, Hiroko Tsukamura

{"title":"Sex difference in developmental changes in visualized Kiss1 neurons in newly generated Kiss1-Cre rats.","authors":"Koki Yamada, Mayuko Nagae, Tetsuya Mano, Hitomi Tsuchida, Safiullah Hazim, Teppei Goto, Makoto Sanbo, Masumi Hirabayashi, Naoko Inoue, Yoshihisa Uenoyama, Hiroko Tsukamura","doi":"10.1262/jrd.2023-019","DOIUrl":"10.1262/jrd.2023-019","url":null,"abstract":"Hypothalamic kisspeptin neurons are master regulators of mammalian reproduction via direct stimulation of gonadotropin-releasing hormone and consequent gonadotropin release. Here, we generated novel Kiss1 (kisspeptin gene)-Cre rats and investigated the developmental changes and sex differences in visualized Kiss1 neurons of Kiss1-Cre-activated tdTomato reporter rats. First, we validated Kiss1-Cre rats by generating Kiss1-expressing cell-specific Kiss1 knockout (Kiss1-KpKO) rats, which were obtained by crossing the current Kiss1-Cre rats with Kiss1-floxed rats. The resulting male Kiss1-KpKO rats lacked Kiss1 expression in the brain and exhibited hypogonadotropic hypogonadism, similar to the hypogonadal phenotype of global Kiss1 KO rats. Histological analysis of Kiss1 neurons in Kiss1-Cre-activated tdTomato reporter rats revealed that tdTomato signals in the anteroventral periventricular nucleus (AVPV) and arcuate nucleus (ARC) were not affected by estrogen, and that tdTomato signals in the ARC, AVPV, and medial amygdala (MeA) were sexually dimorphic. Notably, neonatal AVPV tdTomato signals were detected only in males, but a larger number of tdTomato-expressing cells were detected in the AVPV and ARC, and a smaller number of cells in the MeA was detected in females than in males at postpuberty. These findings suggest that Kiss1-visualized rats can be used to examine the effect of estrogen feedback mechanisms on Kiss1 expression in the AVPV and ARC. Moreover, the Kiss1-Cre and Kiss1-visualized rats could be valuable tools for further detailed analyses of sexual differentiation in the brain and the physiological role of kisspeptin neurons across the brain in rats.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"227-238"},"PeriodicalIF":1.8,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e0/6b/jrd-69-227.PMC10602768.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9888239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hatchability evaluation of bovine IVF embryos using OCT-based 3D image analysis. 使用基于OCT的3D图像分析对牛IVF胚胎的孵化性评估。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-10-20 Epub Date: 2023-08-11 DOI: 10.1262/jrd.2023-009

Yasumitsu Masuda, Ryo Hasebe, Yasushi Kuromi, Mitsugu Hishinuma, Tetsuya Ohbayashi, Ryo Nishimura

{"title":"Hatchability evaluation of bovine IVF embryos using OCT-based 3D image analysis.","authors":"Yasumitsu Masuda, Ryo Hasebe, Yasushi Kuromi, Mitsugu Hishinuma, Tetsuya Ohbayashi, Ryo Nishimura","doi":"10.1262/jrd.2023-009","DOIUrl":"10.1262/jrd.2023-009","url":null,"abstract":"Although embryo transfer is widely applied in cattle, many of the transferred embryos do not result in pregnancy. To determine a new parameter for bovine embryo evaluation, we investigated the relationships between in vitro hatchability and embryo morphological parameters using optical coherence tomography (OCT) that we established recently. Bovine embryos were obtained from Japanese Black cattle by in vitro fertilization (IVF). The quality of the blastocysts was examined under an inverted microscope and confirmed as Codes 1-3 according to the IETS standards for embryo evaluation. The OCT images of the embryos were captured on Day 7 after IVF, and the embryos were cultured until Day 9 to determine their hatchability. During OCT, the embryos were irradiated with near-infrared light for a few minutes to obtain three-dimensional images. In total, 22 parameters were assessed for each of the 42 embryos, of which 25 hatched (H embryos) and 17 did not (NH embryos). The thickness of the trophectoderm (TE) and TE+zona pellucida (ZP) was lesser, and the volumes of the TE, ZP, blastocoel, and whole embryo and blastocoel diameter were greater in the H embryos than in the NH embryos. PCA identified that the increase in the blastocoel-related value along with the decrease in the thickness-related value of the TE and/or ZP could be indicators for evaluating the hatchability of bovine IVF embryos. These results support the idea that OCT-captured structural data of blastocyst-stage embryos can be used as a potential model to predict the quality of bovine embryos.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"239-245"},"PeriodicalIF":1.8,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/15/c2/jrd-69-239.PMC10602767.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9990699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in daily milk production during early pregnancy alter placental characteristics and neonatal metabolic amino acid levels in dairy cows. 妊娠早期每日产奶量的差异会改变奶牛的胎盘特征和新生儿代谢氨基酸水平。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-10-20 Epub Date: 2023-08-16 DOI: 10.1262/jrd.2023-004

Riku Mashimo, Sayaka Ito, Chiho Kawashima

{"title":"Differences in daily milk production during early pregnancy alter placental characteristics and neonatal metabolic amino acid levels in dairy cows.","authors":"Riku Mashimo, Sayaka Ito, Chiho Kawashima","doi":"10.1262/jrd.2023-004","DOIUrl":"10.1262/jrd.2023-004","url":null,"abstract":"We investigated the effects of differences in milk production during early pregnancy on placental characteristics at full term, calf birth weights, and their metabolic status. Thirty-four Holstein cows were categorized into three groups (Low, n = 9; Middle, n = 16; High, n = 9) based on the quartile of average daily 4% fat-corrected milk production during early pregnancy. The High group showed higher milk component production than the other groups (P < 0.05) during early and mid-pregnancy. Although most placental characteristics did not differ significantly among the groups, cows in the High group had larger individual cotyledons and fewer medium-sized cotyledons than those in the Low group (P < 0.05). Plasma amino acid concentrations of calves in the Low and High groups were significantly higher than those of calves in the Middle group, although calf birth weights were similar among the groups. Furthermore, cows in the Low group had longer dry periods than those in the High (P = 0.004) and Middle (P = 0.058) groups. This suggests that cows in the Low group may have provided more amino acids to the fetus because of low lactation and long dry periods. Conversely, cows in the High group required more energy for lactation during early pregnancy, which can reduce nutrient availability to the placenta and fetus; however, increasing individual cotyledonary sizes during late pregnancy may ensure that the same amounts of amino acids as those in cows in the Low group are supplied to the fetus, recovering the birth weights.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"254-260"},"PeriodicalIF":1.8,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/35/bf/jrd-69-254.PMC10602765.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10389347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress. 5-氨基乙酰丙酸/柠檬酸亚铁钠通过减少氧化应激改善热应激牛卵母细胞的质量。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-10-20 Epub Date: 2023-08-21 DOI: 10.1262/jrd.2023-038

Omnia Elgendy, Go Kitahara, Kentaro Yamada, Shin Taniguchi, Takeshi Osawa

{"title":"5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress.","authors":"Omnia Elgendy, Go Kitahara, Kentaro Yamada, Shin Taniguchi, Takeshi Osawa","doi":"10.1262/jrd.2023-038","DOIUrl":"10.1262/jrd.2023-038","url":null,"abstract":"A high temperature-humidity index during summer has deleterious effects on mitochondrial function, reducing oocyte developmental competence. 5-Aminolevulinic acid (5-ALA) and sodium ferrous citrate (SFC) are both known to support mitochondrial function and have strong anti-oxidant and anti-apoptotic activities. This study aimed to determine the mechanism of action of 5-ALA/SFC on oocyte quality. Bovine oocytes were collected from medium-sized follicles during summer (July-September, temperature-humidity index:76.6), cultured with 0, 1, 2, 4, and 8 µM 5-ALA with SFC at a molar ratio of 1:0.125, fertilized, and cultured for 10 days. The addition of 8/1 µM 5-ALA/SFC had a deleterious effect on oocyte cleavage rate in comparison with control oocytes, but did not affect the blastocyst rate, while 1/0.125 µM 5-ALA/SFC had a significantly higher increase in blastocyst rate than 8/1 µM 5-ALA/SFC. The addition of 1/0.125 and 2/0.25 µM 5-ALA/SFC improved oocyte quality by increasing the mitochondrial distribution pattern and metaphase-II oocytes, reducing reactive oxygen species and upregulating nuclear factor erythroid-2-related factor 2, heme oxygenase-1, and superoxide dismutase-1 in oocytes, and nuclear factor erythroid-2-related factor 2 and mitochondrial transcription factor A in cumulus cells. These results indicate that 1/0.125 and 2/0.25 µM 5-ALA/SFC may support oocyte quality and developmental competence and provide anti-oxidant actions in cumulus-oocyte complexes.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"261-269"},"PeriodicalIF":1.8,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/f0/jrd-69-261.PMC10602763.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10028973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of ACVR1B/TGFBR1/ACVR1C signaling inhibition on oocyte and granulosa cell development during in vitro growth culture. ACVR1B/TGFBR1/ACVR1C信号抑制对体外生长培养过程中卵母细胞和颗粒细胞发育的影响。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-10-20 Epub Date: 2023-09-19 DOI: 10.1262/jrd.2023-041

Yuki Akimoto, Wataru Fujii, Kunihiko Naito, Koji Sugiura

{"title":"The effect of ACVR1B/TGFBR1/ACVR1C signaling inhibition on oocyte and granulosa cell development during in vitro growth culture.","authors":"Yuki Akimoto, Wataru Fujii, Kunihiko Naito, Koji Sugiura","doi":"10.1262/jrd.2023-041","DOIUrl":"10.1262/jrd.2023-041","url":null,"abstract":"The signals of the transforming growth factor β (TGF-β) superfamily play a critical role in follicular development in mammals. ACVR1B/TGFBR1/ACVR1C receptors mediate the signaling of several TGF-β superfamily ligands in granulosa cells. Although the requirement for ACVR1B/TGFBR1/ACVR1C receptor signaling in follicular development has been confirmed using mutant mouse models, the detailed roles of the signaling in granulosa cell and oocyte development have not been clearly defined. In the present study, we examined the requirement for ACVR1B/TGFBR1/ACVR1C receptor signaling in granulosa cells using an in vitro growth culture of oocyte-granulosa cell complexes (OGCs) and SB431542, a potent inhibitor of the receptor signaling. Although cumulus-oocyte complexes isolated from the control OGCs were able to undergo cumulus expansion, those isolated from OGCs grown with the inhibitor were not competent, even in the presence of in vivo-grown oocytes. The diameter of the oocytes in the SB431542-treated OGCs was comparable with that of the control; however, these oocytes were not competent for complete meiotic maturation or preimplantation development. Therefore, ACVR1B/TGFBR1/ACVR1C receptor signaling is not required for oocytes to increase their volume but is essential for the normal development of cumulus cells and oocyte developmental competence.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"270-278"},"PeriodicalIF":1.8,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2f/a5/jrd-69-270.PMC10602769.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10656894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enkephalin-δ opioid receptor signaling partly mediates suppression of LH release during early lactation in rats. 脑啡肽δ阿片受体信号部分介导大鼠哺乳期早期LH释放的抑制。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-08-11 DOI: 10.1262/jrd.2023-006

Hitomi Tsuchida, Marina Takizawa, Miku Nonogaki, Naoko Inoue, Yoshihisa Uenoyama, Hiroko Tsukamura

{"title":"Enkephalin-δ opioid receptor signaling partly mediates suppression of LH release during early lactation in rats.","authors":"Hitomi Tsuchida, Marina Takizawa, Miku Nonogaki, Naoko Inoue, Yoshihisa Uenoyama, Hiroko Tsukamura","doi":"10.1262/jrd.2023-006","DOIUrl":"https://doi.org/10.1262/jrd.2023-006","url":null,"abstract":"Gonadal function is often suppressed during lactation in mammals including rodents, ruminants, and primates. This suppression is thought to be mostly due to the inhibition of the tonic (pulsatile) release of gonadotropin-releasing hormone (GnRH) and consequent gonadotropin. Accumulating evidence suggests that kisspeptin neurons in the arcuate nucleus (ARC) play a critical role in the regulation of pulsatile GnRH/gonadotropin release, and kisspeptin mRNA (Kiss1) and/or kisspeptin expression in the ARC are strongly suppressed by the suckling stimuli in lactating rats. This study aimed to examine whether the central enkephalin-δ-opioid receptor (DOR) signaling mediates the suckling-induced suppression of luteinizing hormone (LH) release in lactating rats. Central administration of a selective DOR antagonist increased the mean plasma LH levels and baseline of LH pulses in ovariectomized lactating mother rats compared to vehicle-injected control dams on day 8 of lactation without affecting the number of Kiss1-expressing cells and the intensity of Kiss1 mRNA signals in the ARC. Furthermore, the suckling stimuli significantly increased the number of enkephalin mRNA (Penk)-expressing cells and the intensity of Penk mRNA signals in the ARC compared to non-lactating control rats. Collectively, these results suggest that central DOR signaling, at least in part, mediates the suppression of LH release induced by suckling stimuli in lactating rats via indirect and/or direct inhibition of ARC kisspeptin neurons.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 4","pages":"192-197"},"PeriodicalIF":1.8,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/92/14/jrd-69-192.PMC10435526.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10419206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A combined treatment with progesterone, anti-inhibin serum, and equine chorionic gonadotropin improves number of ovulated oocytes in young C57BL/6J mice. 黄体酮、抗抑制素血清和马绒毛膜促性腺激素联合治疗可提高幼年C57BL/6J小鼠的排卵卵母细胞数量。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-08-11 DOI: 10.1262/jrd.2023-036

Atsuko Kageyama, Mizuho Tsuchiya, Jumpei Terakawa, Junya Ito, Naomi Kashiwazaki

{"title":"A combined treatment with progesterone, anti-inhibin serum, and equine chorionic gonadotropin improves number of ovulated oocytes in young C57BL/6J mice.","authors":"Atsuko Kageyama, Mizuho Tsuchiya, Jumpei Terakawa, Junya Ito, Naomi Kashiwazaki","doi":"10.1262/jrd.2023-036","DOIUrl":"https://doi.org/10.1262/jrd.2023-036","url":null,"abstract":"Superovulation procedures are routinely and widely used in mouse reproductive technology. Previous studies have shown that a large number of oocytes can be obtained from adult mice (> 10 weeks old) using a combined treatment with progesterone (P4) and anti-inhibin serum (AIS). However, these effects have not been fully investigated in young (4 weeks) C57BL/6J mice. Here, we found that a modified superovulation protocol (combined treatment with P4, AIS, eCG (equine chorionic gonadotropin), and hCG (human chorionic gonadotropin); P4D2-Ae-h) improved the number of oocytes compared to the control (eCG and hCG) (39.7 vs. 21.3 oocytes/mouse). After in vitro fertilization, pronuclear formation rates were 69.3% (P4D2-Ae-h group) and 66.2% (control group). After embryo transfer, 46.4% (116/250) of the embryos in the P4D2-Ae-h group successfully developed to term, which was comparable to the control group (42.9%; 123/287 embryos). In conclusion, our protocol (P4D2-Ae-h) was effective for superovulation in young C57BL/6J mice.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"69 4","pages":"223-226"},"PeriodicalIF":1.8,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a5/72/jrd-69-223.PMC10435527.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0