黄体酮对大鼠精子过度激活的增强作用。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-10-20 Epub Date: 2023-09-10 DOI:10.1262/jrd.2023-040

Yuki Miyazawa, Masakatsu Fujinoki

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引用次数: 0

摘要

孕酮（P）是一种众所周知的过度激活增强剂，与体外受精（IVF）的成功有关。在这项研究中，我们检测了P-增强的过度激活是否影响大鼠体外受精的成功。当大鼠精子暴露于10ng/ml、20ng/ml和40ng/ml P时，20ng/ml P通过膜孕酮受体增强过度激活。此外，磷脂酶C、跨膜腺苷酸环化酶和蛋白激酶A调节20ng/ml P对过度激活的增强。然而，20ng/ml的P并不影响IVF的成功。这些结果表明，20ng/ml P通过非基因组途径增强大鼠精子的过度激活。由于P的浓度在发情周期中发生变化，似乎大鼠精子对输卵管环境的反应是过度激活的。然而，20ng/ml P的作用似乎并不能使精子完全获能。

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Enhancement of rat spermatozoal hyperactivation by progesterone.

Progesterone (P) is a well-known enhancer of hyperactivation which is associated with the success of in vitro fertilization (IVF). In this study, we examined whether P-enhanced hyperactivation affected IVF success in rats. When rat spermatozoa were exposed to 10, 20, and 40 ng/ml P, 20 ng/ml P enhanced hyperactivation via the membrane progesterone receptor. In addition, the enhancement of hyperactivation by 20 ng/ml P was regulated by phospholipase C, transmembrane adenylate cyclase, and protein kinase A. However, 20 ng/ml P did not affect IVF success. These results suggest that 20 ng/ml P enhances rat spermatozoal hyperactivation through non-genomic pathways. Because the concentration of P changes during the estrous cycle, it seems that rat spermatozoa are hyperactivated in response to the oviductal environment. However, the effect of 20 ng/ml P does not seem to fully capacitate spermatozoa.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.