黄体酮对大鼠精子过度激活的增强作用。

IF 1.9 4区生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Reproduction and Development Pub Date : 2023-10-20 Epub Date: 2023-09-10 DOI:10.1262/jrd.2023-040

Yuki Miyazawa, Masakatsu Fujinoki

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引用次数: 0

摘要

孕酮（P）是一种众所周知的过度激活增强剂，与体外受精（IVF）的成功有关。在这项研究中，我们检测了P-增强的过度激活是否影响大鼠体外受精的成功。当大鼠精子暴露于10ng/ml、20ng/ml和40ng/ml P时，20ng/ml P通过膜孕酮受体增强过度激活。此外，磷脂酶C、跨膜腺苷酸环化酶和蛋白激酶A调节20ng/ml P对过度激活的增强。然而，20ng/ml的P并不影响IVF的成功。这些结果表明，20ng/ml P通过非基因组途径增强大鼠精子的过度激活。由于P的浓度在发情周期中发生变化，似乎大鼠精子对输卵管环境的反应是过度激活的。然而，20ng/ml P的作用似乎并不能使精子完全获能。

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Enhancement of rat spermatozoal hyperactivation by progesterone.

Progesterone (P) is a well-known enhancer of hyperactivation which is associated with the success of in vitro fertilization (IVF). In this study, we examined whether P-enhanced hyperactivation affected IVF success in rats. When rat spermatozoa were exposed to 10, 20, and 40 ng/ml P, 20 ng/ml P enhanced hyperactivation via the membrane progesterone receptor. In addition, the enhancement of hyperactivation by 20 ng/ml P was regulated by phospholipase C, transmembrane adenylate cyclase, and protein kinase A. However, 20 ng/ml P did not affect IVF success. These results suggest that 20 ng/ml P enhances rat spermatozoal hyperactivation through non-genomic pathways. Because the concentration of P changes during the estrous cycle, it seems that rat spermatozoa are hyperactivated in response to the oviductal environment. However, the effect of 20 ng/ml P does not seem to fully capacitate spermatozoa.

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来源期刊

Journal of Reproduction and Development 生物-奶制品与动物科学

CiteScore

3.70

自引率

11.10%

发文量

审稿时长

2 months

期刊介绍： Journal of Reproduction and Development (JRD) is the official journal of the Society for Reproduction and Development, published bimonthly, and welcomes original articles. JRD provides free full-text access of all the published articles on the web. The functions of the journal are managed by Editorial Board Members, such as the Editor-in-Chief, Co-Editor-inChief, Managing Editors and Editors. All manuscripts are peer-reviewed critically by two or more reviewers. Acceptance is based on scientific content and presentation of the materials. The Editors select reviewers and correspond with authors. Final decisions about acceptance or rejection of manuscripts are made by the Editor-in-Chief and Co-Editor-in-Chief.