Journal of Reproduction and Development最新文献

{"title":"Systemic LPS exposure suppresses pulsatile LH secretion and impairs hepatic function in goats, accompanied by immune activation and stress responses.","authors":"Wenhao Cui, Misato Kikuzawa, Tomotsugu Takahashi, Keiichi Kariya, Junyou Li, Sho Nakamura, Satoshi Ohkura, Josef Johann Gross, Hiroko Tsukamura, Fuko Matsuda, Fumie Magata","doi":"10.1262/jrd.2026-010","DOIUrl":"10.1262/jrd.2026-010","url":null,"abstract":"<p><p>Uterine inflammatory diseases are a major cause of subfertility in both humans and domestic animals. Lipopolysaccharide (LPS) is a key mediator of uterine inflammation and reproductive dysfunction. Although the acute suppressive effects of LPS on the hypothalamic-pituitary-gonadal axis are well documented, its sustained effects under persistent inflammation remain unclear. This study investigated the effects of systemic LPS exposure on reproductive and hepatic function, immune activation, and stress responses in estradiol-treated ovariectomized goats. Animals received either a single intravenous (i.v.) bolus injection of LPS or a continuous i.v. infusion. Serial blood samples were collected for 8 h to evaluate plasma concentrations of LPS, interleukin (IL)-1β, cortisol, and liver enzymes, as well as pulsatile luteinizing hormone (LH) secretion. Rectal temperature increased during the early phase after LPS administration. Plasma LPS concentrations increased several hours after LPS administration, with a biphasic elevation observed during continuous LPS infusion. IL-1β and cortisol increased within 1 h of LPS administration, coinciding with LH pulse suppression and preceding the rise in plasma LPS. Gamma-glutamyltransferase (GGT) increased within 3 h and remained elevated after LPS administration. Aspartate transaminase (AST) increased at the end of the continuous LPS infusion experiment. Systemic LPS administration significantly suppressed pulsatile LH secretion compared with saline treatment. These findings suggest that LPS-driven systemic immune activation and/or stress-related endocrine responses may contribute to suppression of pulsatile LH secretion, and that progressive hepatic dysfunction during continuous LPS exposure may contribute to sustained circulating LPS and prolonged reproductive dysfunction.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"794-804"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13677209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148430634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of laminin-treated membrane culture inserts on the growth of bovine oocyte-granulosa cell complexes collected from early antral follicles.","authors":"Yuji Hirao, Satoshi Akagi","doi":"10.1262/jrd.2026-030","DOIUrl":"10.1262/jrd.2026-030","url":null,"abstract":"<p><p>Growing bovine oocytes represent a potential source of embryos, but this has not been adopted in practice. Although several offspring were produced following a two-week culture period, further optimization of the culture system is required. In vivo, the outermost granulosa cell layer is in direct contact with the basement membrane (BM); however, this interaction is absent in cultured oocyte-granulosa cell complexes (OGCs). Since the BM is known to have regulatory roles, this study examined the effects of BM components on oocyte growth by treating membrane inserts with laminin, a major BM constituent, or with the laminin-containing protein mixture Matrigel^®. OGCs containing growing oocytes (90-106 μm in diameter) were cultured from Day 0 to Day 14 on plain membranes or on membranes with spots of Matrigel or laminin. A modified minimum essential medium α supplemented with 5% fetal bovine serum, 4% polyvinylpyrrolidone (average molecular weight, 360,000), steroid hormones, and 3 mM hypoxanthine was used. Granulosa cell migration was more pronounced in the treated spots, particularly in the Matrigel-treated group where migration extended across the entire treated area. Oocyte volume increased to 147% in the control group, 158% in the Matrigel group, and 160% in the laminin group, with Day 1 values normalized to 100%. Oocytes cultured on laminin-treated membranes were significantly larger than those in the control group (P < 0.05). Following in vitro fertilization, approximately 5% of the oocytes in each group developed to the blastocyst stage. Overall, the interaction between granulosa cells and laminin-treated membranes positively influenced the growth of bovine oocytes in vitro.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"747-755"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13677169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148271866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Timed administration of llama-purified Beta-Nerve Growth Factor (βNGF) is associated with changes in steroidogenic and LH receptor gene expression in dairy heifers undergoing hormonal estrous synchronization treatments.","authors":"Marcelo H Ratto, Gerardo López García, Ximena Valderrama, César Ulloa-Leal, Gonzalo Gajardo, Héctor Zapata-Carmona, Luis Paiva, Margherita Maranesi, Rodolfo Ungerfeld","doi":"10.1262/jrd.2026-096","DOIUrl":"10.1262/jrd.2026-096","url":null,"abstract":"<p><p>The beta-nerve growth factor (β-NGF), a seminal plasma protein with established luteotrophic effects in camelids and cattle, has been proposed as a modulator of periovulatory follicular function. The optimization of the steroidogenic environment of the preovulatory follicle is critical for oocyte maturation, corpus luteum formation, and embryo quality. However, the molecular actions of β-NGF on granulosa cells (GCs) during the periovulatory period in dairy heifers remain poorly defined. This study evaluated the effects of systemic administration of heterologous β-NGF (purified from llama seminal plasma) on the expression of steroidogenic enzymes in GCs from preovulatory follicles in estrus-synchronized Holstein heifers. Animals received 1 mg of β-NGF intramuscularly on Day 9 (estradiol benzoate administration) or Day 10 (fixed-time artificial insemination) of a progesterone (P4)/estradiol (E2)-based synchronization protocol. The follicular fluid and GCs were collected via transvaginal ultrasound-guided aspiration for hormone quantification and gene expression analysis. β-NGF treatment on Day 9 significantly upregulated Lutenizing hormone/choriogonadotropin receptor (LHCGR) and key steroidogenic enzymes, including 3β-hydroxysteroid dehydrogenase (HSD3B), Cytochrome P450 family 11 subfamily A member 1 (CYP11A1), 17β-hydroxysteroid dehydrogenase (HSD17B), and Cytochrome P450 family 19 subfamily A member 1 (CYP19A1), whereas no transcriptional effects were observed when NGF was administered on Day 10, indicating a timing-dependent regulation of GCs differentiation. In contrast, Growth hormone receptor GHR and Steroidogenic acute regulatory protein expression levels remained unaffected, supporting the selectivity of NGF-induced transcriptional modulation. The follicular fluid P4/E2 ratios were not significantly altered by NGF treatment. These findings indicate that β-NGF increased steroidogenic and LHCGR gene abundance of GCs in a timing-dependent manner, suggesting that NGF may represent a potential modulator of the preovulatory follicle function in dairy cattle.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"805-812"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13677206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of preimplantation genomic selection using in vitro-produced embryos in Japanese Black cattle.","authors":"Shoya Nakatsu, Takashi Fujii, Haruka Shimizu, Hiromi Suzuki, Takeshi Koyama, Ken Sawai","doi":"10.1262/jrd.2026-061","DOIUrl":"https://doi.org/10.1262/jrd.2026-061","url":null,"abstract":"<p><p>Preimplantation genomic selection (PGS) using single nucleotide polymorphism (SNP) genotyping can be used to accelerate genetic improvement in cattle, but the technical components for in vitro-produced (IVP) embryos remain unvalidated in Japanese Black cattle; we aimed to address this gap in the present study. In the technical validation experiments, biopsies of approximately 15 cells and 5 cells achieved median call rates of 97.8% and 95.2%, respectively, with a median SNP genotype concordance of 99.9% with the corresponding embryos in both groups. Among biopsied IVP blastocysts, vitrification preserved a significantly higher cell number than slow freezing. In Japanese Black IVP embryos, call rates ≥ 90% were achieved in 88.5%, variation in genomic estimated breeding values (GEBVs) was observed among full-sibling embryos, and the conception rate after vitrification and transfer was 24.0%. At biopsied-cell call rates ≥ 90%, GEBV ranking was preserved between biopsied cells and the corresponding calves. These findings support the potential utility of PGS using IVP embryos as a breeding tool for Japanese Black cattle.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149030770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing global chromatin accessibility alone is insufficient to enhance global transcription during mouse zygotic gene activation.","authors":"Sana Nakamae, Masatoshi Ooga","doi":"10.1262/jrd.2026-110","DOIUrl":"https://doi.org/10.1262/jrd.2026-110","url":null,"abstract":"<p><p>In mice, zygotic gene activation (ZGA) occurs in two waves: a minor wave at the 1-cell stage and a major wave at the 2-cell stage. At the 1-cell stage, chromatin is highly decondensed and transcriptionally permissive; however, global transcription is low. At the 2-cell stage, global transcription increases with chromatin compaction. Thus, the early embryo departs from the usual association between open chromatin and active transcription. Open chromatin in the 1-cell embryo is regarded as a hallmark of totipotency; however, its contribution to transcription remains unclear. To address this issue, whether increasing accessibility is sufficient to enhance global transcription and whether the response depends on the mechanism of chromatin opening were investigated. Therefore, global chromatin accessibility was increased at each developmental stage using two mechanistically distinct perturbations, KDM4D-mediated removal of H3K9me3 and trichostatin A (TSA)-mediated histone hyperacetylation, and the resulting accessibility and transcription were evaluated by DNase-TUNEL and 5-ethynyl uridine incorporation, respectively. At the 1-cell stage, both perturbations increased accessibility but not global transcription. KDM4D decreased transcription in the maternal pronucleus, whereas TSA reduced transcription in both pronuclei. At the 2-cell stage, KDM4D increased accessibility without altering global transcription. The simultaneous removal of H3K9me3 and H3K27me3 did not increase global transcription. In contrast, TSA increased global accessibility and global transcription. Thus, increasing global chromatin accessibility alone was insufficient to enhance global transcription and could even reduce it. The transcriptional response depended on the mechanism of chromatin perturbation. Furthermore, maternally enriched H3K9me3 did not account for the lower transcriptional activity in the maternal pronucleus.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149030843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal reproductive and metabolic changes in male Pacific white-sided dolphins (Aethalodelphis obliquidens).","authors":"Chika Shirakata, Miyuki Sumi, Yuki Yamamoto, Kentaro Nagaoka","doi":"10.1262/jrd.2026-097","DOIUrl":"https://doi.org/10.1262/jrd.2026-097","url":null,"abstract":"<p><p>Seasonal reproductive dynamics were investigated in two adult male Pacific white-sided dolphins (Aethalodelphis obliquidens, formerly Lagenorhynchus obliquidens) maintained at the Enoshima Aquarium over a two-year period (December 2018-November 2020). Serum testosterone (T), testicular cross-sectional area (TS), and pixel intensity (PI), an index of testicular echogenicity, were measured along with rectal body temperature at two depths (shallow, 15 cm; deep, 30 cm). Untargeted serum metabolomic profiling was performed using gas chromatography-mass spectrometry (GC-MS). In both individuals, T showed a clear seasonal pattern, with elevations lasting approximately one month during spring and summer, followed by prolonged increases in TS (approximately three months) and PI (three-four months). This temporal sequence was consistent across both years of observation. The T, TS, PI, and photoperiod were strongly and positively correlated with one another (Spearman's rs > 0.74, P < 0.01). Shallow-body temperature varied seasonally with water temperature, whereas deep-body temperature remained relatively stable. GC-MS detected 131 metabolites, and partial least squares discriminant analysis revealed a separation between high-T (> 10 ng/ml) and low-T (< 1 ng/ml) states. The four metabolites with the highest variable importance in projection scores were myoinositol, palmitoleic acid, L-tyrosine, and L-phenylalanine. Myoinositol and palmitoleic acid were positively correlated with the reproductive indices, whereas L-tyrosine and L-phenylalanine were negatively correlated, suggesting coordinated shifts in inositol, lipid, and amino acid metabolism during reproductive activation. To the best of our knowledge, this is the first study to integrate endocrine, ultrasonographic, thermal, and metabolomic approaches to characterize male reproductive seasonality in this species.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic profiling of sperm with differential freezability in Wannan Black pig.","authors":"Wenjie Yu, Yi Li, Wenzhuo Wang, Lanruo Wang, Zhuoyuan Zhang, Liangyu Cai, Zongjun Yin, Xiaodong Zhang, Jason G Knott, Yunhai Zhang, Zubing Cao","doi":"10.1262/jrd.2026-056","DOIUrl":"https://doi.org/10.1262/jrd.2026-056","url":null,"abstract":"<p><p>Sperm freezability varies considerably among pigs owing to genetic, husbandry, and management differences. Boar sperm cryopreservation remains underutilized owing to its inconsistent freezability. Semen with good freezability (GF) increases sow litter size and reduces breeding costs. Previous studies identified freezability biomarkers in cattle, sheep, and pigs. To comprehensively investigate these biomarkers, we conducted proteomic profiling of sperm with poor freezability (PF) and GF in Wannan Black pigs. We detected 2,485 proteins, identifying 337 significantly differentially expressed proteins (DEPs). Gene Ontology annotation revealed the involvement of DEPs in crucial processes, such as protein homeostasis maintenance, zona pellucida binding, polyspermy blockade, and ATPase activation regulation. Its molecular functions include immune signaling and oxidative stress defense. KEGG pathway analysis highlighted DEPs enrichment in seminal adhesion complexes, nucleic acid helicase families, and mitochondrial membrane protein clusters. Subcellular localization showed predominant DEPs distribution in the cytoplasm, extracellular matrix, nucleus, mitochondria, and plasma membranes. Western blotting validation of differentially expressed proteins (HSP90AA1, ACE, ANXA2, DEFB1, and S100A8) demonstrated results consistent with the proteomic sequencing data. This study delineates the key proteomic differences underlying boar sperm freezability, providing insights into refining cryopreservation protocols and augmenting swine reproductive efficiency.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LRRC37A is an acrosomal protein required for acrosome integrity and sperm head morphogenesis.","authors":"Keisuke Shimada, Yumiao Qiu, Yuki Kaneda, Anh Hoang Pham, Masahito Ikawa","doi":"10.1262/jrd.2026-071","DOIUrl":"https://doi.org/10.1262/jrd.2026-071","url":null,"abstract":"<p><p>Fertilization depends on the proper formation and function of the acrosome, a specialized organelle essential for sperm-oocyte interaction. Defects in acrosome biogenesis impair fertilization and cause severe male infertility, including globozoospermia. However, the molecular mechanisms underlying acrosome formation and maintenance remain poorly understood. Here, we investigated the roles of the testis-enriched proteins LRRC37 and LRRC37A using knockout (KO) mouse models. While Lrrc37 KO males were fertile with only mild sperm head abnormalities, Lrrc37a KO males were completely infertile and produced round-headed spermatozoa characteristic of globozoospermia. LRRC37A localized to the acrosome, and its loss resulted in abnormal acrosome enlargement accompanied by the accumulation of Golgi- and vesicle-like structures. Acrosome formation in Lrrc37a KO mice appeared normal during early spermiogenesis but became abnormal at later stages, indicating a requirement for LRRC37A in acrosomal structural integrity. These defects were associated with impaired sperm head shaping during spermiogenesis, leading to globozoospermia. Together, these findings establish LRRC37A as an essential factor in maintaining acrosome integrity and sperm head morphogenesis, highlighting its distinct function from LRRC37. This work provides new insight into the molecular basis of acrosome-related infertility and has important implications for understanding the etiology of globozoospermia.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct localization and potential functions of GPR37 signaling mediated by prosaposin and protectin D1 in the rat pituitary gland.","authors":"Ayano Shindo, Morio Azuma, Aki Obara, Kai Kitamura, Aimi Fuyuki, Shouichiro Saito, Kotaro Horiguchi","doi":"10.1262/jrd.2026-055","DOIUrl":"https://doi.org/10.1262/jrd.2026-055","url":null,"abstract":"<p><p>Prosaposin (PSAP), a secreted glycoprotein with neurotrophic activity, and protectin D1, a docosahexaenoic acid-derived lipid mediator, are endogenous ligands of G protein-coupled receptor 37 (GPR37). Although PSAP is expressed in the pituitary gland, the cellular localization and functional significance of PSAP-GPR37 signaling and protectin D1-associated pathways remain unclear. In this study, we investigated the localization and potential functions of PSAP, GPR37, and protectin D1-related factors in the rat pituitary gland, focusing on adult pituitary stem/progenitor cells (CD9/SRY-box transcription factor 2 [SOX2]-positive cells) in the marginal cell layer (MCL) facing the residual lumen of Rathke's pouch. In situ hybridization and immunohistochemistry revealed that PSAP was localized in CD9/SOX2-positive cells in the intermediate lobe-side MCL (IL-MCL) and in subsets of adrenocorticotropic hormone (ACTH)- and growth hormone-producing cells in the anterior lobe (AL) of the pituitary gland. Gpr37 was predominantly detected in CD9/SOX2-positive cells in the IL-MCL and in some ACTH-producing cells. The protectin D1-associated factors, fatty acid transport protein 1 (FATP1/SLC27A1) and arachidonate 15-lipoxygenase (ALOX15), colocalized with CD9/SOX2-positive cells in the IL-MCL. In a pharmacologically induced prolactinoma model, Gpr37, Slc27a1, and Alox15 expression level was upregulated in IL-derived CD9-positive cells. Functional analyses showed that PSAP treatment increased proopiomelanocortin expression in AL-derived CD9-negative cells, whereas protectin D1 treatment increased Sox2 expression and decreased Gpr37 expression in CD9/SOX2-positive cells. These findings suggest that ligand-dependent GPR37 signaling may have distinct roles in the pituitary gland: PSAP-GPR37 signaling may regulate ACTH production, whereas protectin D1-GPR37 signaling may contribute to Sox2 expression in CD9/SOX2-positive cells.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148829610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cavitary corpus luteum: opportunity or threat for bovine reproduction?","authors":"Christian Hanzen, Fernando López-Gatius","doi":"10.1262/jrd.2026-104","DOIUrl":"https://doi.org/10.1262/jrd.2026-104","url":null,"abstract":"<p><p>The homogenous, compact or non-cavitary corpus luteum (CL) is an ovarian structure appearing after ovulation and responsible of progesterone synthesis during the estrous cycle and pregnancy. The cavitary CL is similar to the homogeneous but it presents a more or less developed central cavity. The prevalence of a cavitary CL ranges from 18 to 79% during the period of 6 to 12 days post-ovulation. Most cavities regress throughout the cycle. This review aims to present the state of art of the prevalence, physio-morphological characteristics, diagnostic methods and effects on fertility of the cavitary corpus luteum, most of the aspects remaining a topic of debate. From a clinical perspective, the presence of a CL with a large cavity with a very thin wall, resembling an ovarian follicular/luteal cyst, in absence of a further CL in pregnant cows is also described.</p>","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}