Journal of Reproduction and Development最新文献_第3页

Studies on gestational factors affecting postpartum reproductive performance in dairy cows and the health and reproductive potential of their offspring. 影响奶牛产后生殖性能及子代健康和生殖潜能的妊娠因素研究。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-02-25 DOI: 10.1262/jrd.2026-015

Chiho Kawashima

{"title":"Studies on gestational factors affecting postpartum reproductive performance in dairy cows and the health and reproductive potential of their offspring.","authors":"Chiho Kawashima","doi":"10.1262/jrd.2026-015","DOIUrl":"https://doi.org/10.1262/jrd.2026-015","url":null,"abstract":"Improving and sustaining the health and productivity of dairy cows require integrated strategies that combine management and appropriate nutrition. This review adopts two complementary perspectives: optimizing periparturient management to support high productivity without compromising animal health and improving gestational nutrition to produce healthy replacement heifers. Several studies have examined how maternal nutritional and metabolic status before and after calving influence postpartum reproduction, productivity, placental development, and offspring health. Prepartum energy status is a key determinant of early postpartum ovarian function, with a timely first ovulation associated with adequate insulin-like growth factor-I exposure during follicular development. Insufficient β-carotene intake in energy-prioritized feeding systems adversely affects ovarian resumption. Uterine recovery is also nutrition-sensitive; cows with endometritis exhibit signs of impaired liver function, whereas delayed uterine involution is associated with inefficient ruminal nitrogen utilization. Supplementation with lysine, the first limiting amino acid in corn-based diets, further promotes uterine recovery. From a developmental perspective, maternal insulin resistance is associated with low birth weight and altered endocrine profiles in calves, which are indicative of long-term metabolic vulnerability. In contrast, lysine supplementation during the dry period supports neonatal protein synthesis and growth. Furthermore, in growing primiparous cows, greater milk yield during early pregnancy was associated with reduced anti-Müllerian hormone concentrations in female calves, suggesting a lower ovarian reserve. Collectively, these findings highlight the importance of gestational and peripartum nutritional management not only for improving dairy cow fertility and productivity but also for optimizing the health and reproductive potential of the next generation.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147307157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of timing and morphology of blastomere cleavage on gene expression profiles of bovine in vitro fertilized embryos. 卵裂时间和形态对牛体外受精胚胎基因表达谱的影响。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-02-17 Epub Date: 2025-12-13 DOI: 10.1262/jrd.2025-035

Shunki Ono, Hinano Kozuka, Kota Okubo, Takashi Fujii, Ken Sawai

引用次数: 0

Transgenic Bax gene efficiently induces lethality in mouse early embryos. 转Bax基因可有效诱导小鼠早期胚胎致死性。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-02-17 Epub Date: 2025-12-20 DOI: 10.1262/jrd.2025-054

Yuzuki Goto, Takuto Yamamoto, Masahiro Sakata, Satoshi Mashiko, Daiki Shikata, Shinnosuke Honda, Naojiro Minami, Shuntaro Ikeda

{"title":"Transgenic Bax gene efficiently induces lethality in mouse early embryos.","authors":"Yuzuki Goto, Takuto Yamamoto, Masahiro Sakata, Satoshi Mashiko, Daiki Shikata, Shinnosuke Honda, Naojiro Minami, Shuntaro Ikeda","doi":"10.1262/jrd.2025-054","DOIUrl":"10.1262/jrd.2025-054","url":null,"abstract":"Apoptosis is an essential physiological process involved in embryonic development, immune responses, and tissue homeostasis. Despite many studies on pro-apoptotic genes, few reports have directly compared the lethality-inducing potential between them under comparable conditions. In this study, we evaluated the lethality-inducing potential of three representative pro-apoptotic genes, Bax, Casp3, and Casp9, in mouse early embryos under defined conditions using the doxycycline (Dox)-inducible tetracycline-regulated gene expression system in combination with the PiggyBac transposon system. All genes were transcriptionally induced by Dox, and Bax showed the strongest lethal effect, followed by Casp9, while Casp3 did not show any effects. Notably, Bax expression severely impaired blastocyst formation and led to the intense accumulation of the DNA damage marker γH2AX, along with a pronounced increase in the apoptotic cells. These findings suggest that introducing upstream apoptotic regulators leads to the more efficient and widespread activation of the apoptotic cascade. Overall, this study is expected to contribute to a deeper understanding of apoptotic mechanisms and future advancements in regenerative medicine, reproductive engineering, and cancer research.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"16-23"},"PeriodicalIF":2.2,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATAD2 deficiency leads to subfertility by impairing spermatogenesis in mice. 在小鼠中，ATAD2缺乏通过损害精子发生导致生育能力低下。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-02-17 Epub Date: 2025-12-25 DOI: 10.1262/jrd.2025-060

Rui Wu, Shuyun Zhao, Qi Yu, Yili Zhao, Yan Han, Rong Liu, ChaoQun Duan

{"title":"ATAD2 deficiency leads to subfertility by impairing spermatogenesis in mice.","authors":"Rui Wu, Shuyun Zhao, Qi Yu, Yili Zhao, Yan Han, Rong Liu, ChaoQun Duan","doi":"10.1262/jrd.2025-060","DOIUrl":"10.1262/jrd.2025-060","url":null,"abstract":"During spermatogenesis, chromatin remodeling regulated by histone modification is essential for spermatogenic cell development, and multiple epigenetic regulators are involved in this process. Recent studies reported that ATAD2 was a newly discovered cancer/testis factor that functioned in chromatin remodeling in somatic cells by binding acetylated histone. However, the physiological role of ATAD2 in spermatogenesis is largely unknown. In this study, we characterized the expression pattern of ATAD2 in mouse testes and found that the highly expressed ATAD2 in the gonads was lowly expressed in meiotic spermatocytes with distinct localization in nucleus, but highly expressed in round spermatids. By generating Atad2 knockout (KO) mice using CRISPR/Cas9 technology, we revealed that ATAD2 deletion leads to failure of DSB repair and chromosome synapsis in spermatocytes and impairs spermiogenesis in spermatids. Atad2 KO mice were subfertile, as characterized by reduced sperm count, impaired motility, and abnormal morphology. RNA-Seq analysis showed that hundreds of genes were dysregulated in Atad2-KO round spermatids. As revealed by GSEA analysis, the gene set related to spermatid development was downregulated, while gene sets related to chromatin binding and positive and negative DNA-templated transcription were upregulated. In conclusion, our results indicate that ATAD2 contributes to meiotic progression and participates in spermiogenesis by regulating RNA transcription in spermatids.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"24-33"},"PeriodicalIF":2.2,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145827926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of a comprehensive metabolomic analysis system for characterizing metabolic alterations in the cauda epididymis of mature and juvenile mice. 成熟和幼年小鼠附睾尾代谢变化的综合代谢组学分析系统的优化。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-02-17 Epub Date: 2026-01-03 DOI: 10.1262/jrd.2025-063

Ryusei Maeda, Satohiro Nakao, Yuichiro Arima, Ayana Shitashimizu, Keisuke Masuda, Naomi Nakagata, Toru Takeo

{"title":"Optimization of a comprehensive metabolomic analysis system for characterizing metabolic alterations in the cauda epididymis of mature and juvenile mice.","authors":"Ryusei Maeda, Satohiro Nakao, Yuichiro Arima, Ayana Shitashimizu, Keisuke Masuda, Naomi Nakagata, Toru Takeo","doi":"10.1262/jrd.2025-063","DOIUrl":"10.1262/jrd.2025-063","url":null,"abstract":"The cauda epididymis protects and stores mature sperm in mammals. Recently, comprehensive transcriptomic and proteomic analyses have been conducted to understand its molecular functions; however, fundamental information on its metabolites has not been reported. In this study, we optimized a system for the comprehensive metabolic analysis of the cauda epididymis in mature and juvenile mice. This system identified 116 and 92 metabolites in mature and juvenile mice, respectively. Comparative analysis revealed that 44 and 13 metabolites were upregulated and downregulated, respectively, in the cauda epididymis of mature and juvenile mice. Based on the identified metabolites, 34 metabolic and unique pathways (mature: four pathways and juvenile: one pathway) were determined. In conclusion, the levels of certain metabolites in the cauda epididymis differed between mature and juvenile mice. These results contribute to understanding of the unique functions of the cauda epididymis based on dynamic changes in metabolites.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"8-15"},"PeriodicalIF":2.2,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum: Ybx1 deficiency impairs spermatid development and male fertility without affecting meiosis in mice: insights into spermatogenesis. 勘误：Ybx1缺乏损害精子发育和雄性生育能力，而不影响小鼠减数分裂：对精子发生的见解。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-01-01 DOI: 10.1262/jrd.2024-108c

Yan Han, Rui Wu, Chaoqun Duan, Jiemin Chen, Xing Deng, Wei Peng, Buzhen Tan

引用次数: 0

Evaluation of tubal patency with hysterosalpingo-contrast sonography using sodium alginate solution and the subsequent fertility outcomes in repeat breeder cattle. 用海藻酸钠溶液评价子宫输卵管造影对重复种牛输卵管通畅的影响及随后的生育结果。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-12-01 Epub Date: 2025-11-02 DOI: 10.1262/jrd.2025-052

Kazuki Ito, Ryuji Hashimoto, Natsumi Endo, Tomomi Tanaka

{"title":"Evaluation of tubal patency with hysterosalpingo-contrast sonography using sodium alginate solution and the subsequent fertility outcomes in repeat breeder cattle.","authors":"Kazuki Ito, Ryuji Hashimoto, Natsumi Endo, Tomomi Tanaka","doi":"10.1262/jrd.2025-052","DOIUrl":"10.1262/jrd.2025-052","url":null,"abstract":"Tubal abnormalities were evaluated on hysterosalpingo-contrast sonography (HyCoSy) using sodium alginate solution as a contrast agent in 18 repeat breeder cattle. Clear contrast enhancement was observed from the uterine horn to the tubal infundibulum in the patent cases, and the percentage of cases with unilateral or bilateral passage disorder was 64.7%. The post-examination artificial insemination conception rate when ovulatory follicles were present on the patent side was 64.3%, which was significantly higher than 12.5% seen when ovulatory follicles were present on the side affected by passage disorder (P < 0.05). The polymorphonuclear leukocyte percentage in the recovered contrast agent was significantly higher on the passage disorder side than on the patent side, whereas there was no significant difference in C-reactive protein concentrations between the patent and passage disorder sides. The present method can be used for diagnosing tubal abnormalities, and chronic inflammatory changes may be related to tubal passage disorder.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"310-314"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145438306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of bovine oocyte transportation system on embryonic quality. 牛卵母细胞运输系统对胚胎质量的影响。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-12-01 Epub Date: 2025-10-04 DOI: 10.1262/jrd.2025-031

Shunsuke Hara, Minori Shida, Kanami Abe, Koumei Shirasuna, Hisataka Iwata

{"title":"Effect of bovine oocyte transportation system on embryonic quality.","authors":"Shunsuke Hara, Minori Shida, Kanami Abe, Koumei Shirasuna, Hisataka Iwata","doi":"10.1262/jrd.2025-031","DOIUrl":"10.1262/jrd.2025-031","url":null,"abstract":"The conventional ovum pick-up method requires oocytes to be transported from local farms to the laboratory, where they undergo nuclear maturation. However, atmospheric conditions for oocyte transportation differ from those for normal oocyte maturation in vitro. In this study, we examined the effects of conventional and modified oocyte transport conditions on oocyte quality and subsequent embryonic development. Cumulus-oocyte complexes were collected from slaughterhouse-derived bovine ovaries and cultured in few drops of medium on plastic plates in a CO2-incubator (Control), in plastic tubes containing medium (C-T) in air, or in tubes containing gellan gum and medium (MC-T) in air. C-T conditions reduced mitochondrial functionality (mitochondrial membrane potential and adenosine triphosphate), lipid content, and DNA methylation but increased mitochondrial DNA copy number and phosphorylated AMP-activated protein kinase (P-AMPK) levels compared to those in control oocytes. Furthermore, RNA sequencing analysis of blastocysts derived from these oocytes revealed that C-T conditions affected mitophagy- and AMPK-signaling-related genes. However, MC-T conditions attenuated these C-T-associated changes. In conclusion, conventional C-T conditions affect oocyte metabolism and alter embryo quality, whereas the use of gellan gum as a substrate ameliorates such adverse effects. The oocyte transportation system is inadequate for embryonic production and can induce epigenetic changes. Modifying these conditions with gellan gum is a useful counter-measure.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"301-309"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel method of correlative light and electron microscopy in cryosectioning of bovine anterior pituitary tissue using NanoSuit CLEM. 应用NanoSuit CLEM进行牛垂体前叶冷冻切片的相关光镜和电镜观察。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-10-09 Epub Date: 2025-08-14 DOI: 10.1262/jrd.2025-025

Hiroya Kadokawa, Hideya Kawasaki

{"title":"A novel method of correlative light and electron microscopy in cryosectioning of bovine anterior pituitary tissue using NanoSuit CLEM.","authors":"Hiroya Kadokawa, Hideya Kawasaki","doi":"10.1262/jrd.2025-025","DOIUrl":"10.1262/jrd.2025-025","url":null,"abstract":"Correlative light-electron microscopy (CLEM) combines fluorescence microscopy and scanning electron microscopy (SEM) to achieve nanoscale resolution while highlighting regions of interest identified by fluorescence microscopy. CLEM is becoming increasingly important in life sciences but traditionally requires highly dried samples to withstand the high vacuum of SEM. The NanoSuit method, which mimics native extracellular substances, was developed to address this limitation by encasing samples in a thin, vacuum-proof membrane, allowing SEM observation of live or wet multicellular organisms. While previous NanoSuit CLEM studies focused on formalin-fixed paraffin-embedded sections and cultured cells, cryosections had not yet been explored. In this study, NanoSuit CLEM with diluted NanoSuit solution was applied to cryosections of bovine anterior pituitary tissue. Secretory granules in gonadotrophs, which constitute less than 12% of anterior pituitary cells, were successfully visualized. However, other organelles remained unobserved due to fixation conditions. Therefore, NanoSuit CLEM enabled visualization of the ultrastructure of important cells in cryosections, even from large animals.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"295-300"},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12511776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144847147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanism of heat stress-induced injury in the immature oocytes of zebrafish (Danio rerio). 热应激诱导斑马鱼未成熟卵母细胞损伤的机制。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-10-09 Epub Date: 2025-09-08 DOI: 10.1262/jrd.2025-050

Yanuar Achadri, Nao Kuwahara, Momoko Kimura, Nami Kanamaru, Kazutsugu Matsukawa, Keisuke Edashige

{"title":"The mechanism of heat stress-induced injury in the immature oocytes of zebrafish (Danio rerio).","authors":"Yanuar Achadri, Nao Kuwahara, Momoko Kimura, Nami Kanamaru, Kazutsugu Matsukawa, Keisuke Edashige","doi":"10.1262/jrd.2025-050","DOIUrl":"10.1262/jrd.2025-050","url":null,"abstract":"Immature zebrafish oocytes are highly susceptible to high temperatures, making it difficult to warm cryopreserved oocytes rapidly. In the present study, we aimed to investigate whether thermosensitive channels, lipid mediators, and ferroptosis are involved in heat stress-induced injury in immature zebrafish oocytes. Oocytes were injected with inhibitors of a heat-sensitive channel (TRPV1) and multiple enzymes-cytosolic phospholipase A2α (cPLA2α), cyclooxygenases (COXs), arachidonate 5-lipoxygenase (ALOX5), and lysophosphatidylcholine acyltransferase 2 (LPCAT2). In addition, a ferroptosis-specific inhibitor was administered. The oocytes were then warmed at 45°C for 15 min, incubated at 25°C for 2 h, and then stained with propidium iodide. When the control oocytes were warmed at 45°C for 15 min, their survival was low (1%-8%). However, the survival of oocytes injected with the TRPV1-specific inhibitor markedly improved (40%), suggesting that TRPV1 activation triggers heat stress injury in oocytes. When a cPLA2α-specific inhibitor was injected, survival of oocytes after warming significantly improved (30%), suggesting that lipid mediators or ferroptosis are involved in heat stress-induced injury in oocytes. In contrast, survival either slightly improved or did not improve when oocytes were injected with specific inhibitors of COXs, ALOX5, and LPCAT2 (16%, 8%, and 3%, respectively). Notably, the ferroptosis-specific inhibitor markedly improved oocyte survival (60%). These results may facilitate methodological advancements in fish oocyte cryopreservation. Additionally, they suggest that ferroptosis is involved in heat stress-induced injury in immature zebrafish oocytes, following TRPV1 activation and subsequent cPLA2α activation.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"266-271"},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12511778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0