Russian Journal of Biological Physics and Chemisrty最新文献_第6页

MODELING OF DNA DAMAGE REPAIR INDUCED BY HEAVY IONS IN MAMMALIAN CELLS 哺乳动物细胞中重离子诱导的DNA损伤修复模型

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-24 DOI: 10.29039/rusjbpc.2022.0560

M. Vasil'eva, A. Bugay, E. Dushanov

引用次数: 0

CONTRAST SENSITIVITY AND EYE MICROTREMOR AS MARKERS OF ADAPTATION AND READAPTATION IN A MODEL EXPERIMENT TO STUDY THE INFLUENCE OF GRAVITY CHANGES 对比敏感度和眼微颤作为适应和再适应的标志，在模型实验中研究重力变化的影响

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-24 DOI: 10.29039/rusjbpc.2022.0571

I. Shoshina, I. Zelenskaya, M. Bekreneva, S. Lyapunov, I. Lyapunov, D. Kotova, E. Tomilovskaya

{"title":"CONTRAST SENSITIVITY AND EYE MICROTREMOR AS MARKERS OF ADAPTATION AND READAPTATION IN A MODEL EXPERIMENT TO STUDY THE INFLUENCE OF GRAVITY CHANGES","authors":"I. Shoshina, I. Zelenskaya, M. Bekreneva, S. Lyapunov, I. Lyapunov, D. Kotova, E. Tomilovskaya","doi":"10.29039/rusjbpc.2022.0571","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0571","url":null,"abstract":"Visual perception plays a crucial role in providing the brain with the information it needs to make decisions, build a picture of the world, and adapt to changing environmental conditions. Under conditions of \"dry\" immersion, which simulates the effects of weightlessness on the human body, contrast sensitivity and tremor eye movements were studied under changing environmental conditions. The study involved 10 volunteers (mean age 30.8±4.6 years). The contrast sensitivity of the visual system was recorded using the method of visocontrastometry. We presented the Gabor elements with a spatial frequency: 0.4; 0.8; 1.0; 3.0; 6.0 and 10.0 cycle/deg. The parameters of eye micromovements, i.e., the amplitude and frequency of eye tremor oscillations, were recorded using an optical system providing high-frequency video recording. The measurements were carried out the day before immersion in the immersion bath, on days 1, 3, 5, and 7 of “dry” immersion, as well as the next day after its completion. A change in contrast sensitivity in the range of low and high spatial frequencies, as well as in the amplitude of eye micromovements, was established. The data obtained today are a new step in the search for methods for an objective assessment of the functional state under changing environmental conditions.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133732808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

POSSIBLE MOLECULAR MECHANISMS OF POLYSACCHARIDES ACTION IN VIRAL DISEASES 多糖在病毒性疾病中作用的可能分子机制

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-24 DOI: 10.29039/rusjbpc.2022.0570

E. Generalov, E. Simonenko, L. Yakovenko

引用次数: 0

THE EFFECT OF LOW-INTENSITY ELECTROMAGNETIC RADIATION OF EXTREMELY HIGH FREQUENCY ON THE INDICATORS OF TISSUE OXIDATIVE METABOLISM 极高频低强度电磁辐射对组织氧化代谢指标的影响

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-24 DOI: 10.29039/rusjbpc.2022.0574

M. Ravaeva, E. Chuyan, I. Cheretaev

{"title":"THE EFFECT OF LOW-INTENSITY ELECTROMAGNETIC RADIATION OF EXTREMELY HIGH FREQUENCY ON THE INDICATORS OF TISSUE OXIDATIVE METABOLISM","authors":"M. Ravaeva, E. Chuyan, I. Cheretaev","doi":"10.29039/rusjbpc.2022.0574","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0574","url":null,"abstract":"The paper considers the indicators of tissue oxidative in rats after 10-fold exposure to low-intensity electromagnetic radiation of the millimeter range. The experiment was carried out on 40 mature male rats of the Wistar line weighing 200-220 g, which were kept in a vivarium with a natural light regime. The animals were divided into 2 groups of 20 rats each. The animals in the first group were biological controls and were in normal vivarium conditions, the animals of the second group were exposed to EHF EMR for 10 sessions daily, in the morning. On the 10th day of EHF exposure, tissue fluorescence indicators were recorded by the diagnostic complex “LAZMA MC” (Modification of LAZMA-MC-III, LAZMA, Moscow, Russia). The results of the study showed that rats exposed to 10-fold EHF exposure had an increase in the values of normalized NADH fluorescence amplitudes and an increase in FAD amplitudes by 82.5% (p<0.05) and 42.6% (p<0.05), as well as redox potential - by 36.9% (p<0.05), respectively, compared to with control. These changes indicate that 10-fold exposure to low-intensity EHF EMR causes an increase in the contribution of aerobic metabolism in rats mainly due to oxidative phosphorylation, leads to activation of the respiratory chain. It can be assumed that these changes in oxidative metabolism are determined primarily by the process of adaptation of microcirculatory blood flow to the needs of tissues for nutrients and the removal of metabolites.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130128325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SYNTHESIS OF LUMINESCENT THERANOSTIC NANOCOMPLEXES BASED ON UPCOVERSION NANOPARTICLES AND RECOMBINANT PROTEINS 基于上转换纳米颗粒和重组蛋白的发光治疗纳米复合物的合成

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-24 DOI: 10.29039/rusjbpc.2022.0573

D. Bausheva, N. Shilyagina, V. Vodeneev, S. Deev, A. Zvyagin, E. Guryev

{"title":"SYNTHESIS OF LUMINESCENT THERANOSTIC NANOCOMPLEXES BASED ON UPCOVERSION NANOPARTICLES AND RECOMBINANT PROTEINS","authors":"D. Bausheva, N. Shilyagina, V. Vodeneev, S. Deev, A. Zvyagin, E. Guryev","doi":"10.29039/rusjbpc.2022.0573","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0573","url":null,"abstract":"Theranostics is a rapidly developing field of biomedicine, aimed at the development of multifunctional agents with diagnostic and therapeutic properties simultaneously. This work describes the production of HER2-specific theranostic nanocomplexes based on upconversion nanoparticles and bifunctional proteins. Inorganic nanocrystals of the NaY0.794Yb0.2Tm0.006F4/NaYF4 core/shell structure were used as a platform for the assembly of the multifunctional complex. Upconversion nanoparticles (UCNP) were shown to have pronounced maxima of photoluminescence emission in the visible and infrared regions of the spectrum, which provides effective in vivo imaging of cells and tissues by optical imaging methods. Covering UCNP with additional shells allows achieving their colloidal stability and attaching additional external modules to them. The recombinant targeting toxin DARPin-LoPE, which has specific toxicity against tumor cells expressing the HER2 receptor, was used as a therapeutic module of the nanocomplexes. The theranostic complex NAF-DARPin-LoPE was shown to be able to selectively bind to tumor cells hyperexpressing the HER2 receptor due to the DARPin guide module included in its composition.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"185 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131816891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DEPENDENCE OF THE BIOELECTRIC POTENTIAL IN THE ROOT ENVIRONMENT ON THE NITROGEN FORM IN THE NUTRIENT SOLUTION 根系环境中生物电势对营养液中氮形态的依赖

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-24 DOI: 10.29039/rusjbpc.2022.0558

T. Kuleshova, P. Zhelnacheva, Z. Gasieva, A. Galushko, G. Panova

{"title":"DEPENDENCE OF THE BIOELECTRIC POTENTIAL IN THE ROOT ENVIRONMENT ON THE NITROGEN FORM IN THE NUTRIENT SOLUTION","authors":"T. Kuleshova, P. Zhelnacheva, Z. Gasieva, A. Galushko, G. Panova","doi":"10.29039/rusjbpc.2022.0558","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0558","url":null,"abstract":"The influence of the nitrogen form (nitrate, amide, nitrate-ammonium) in the composition of the nutrient solution on the electrical characteristics of a bioelectrochemical system based on the electrogenic properties of lettuce was studied. The dynamics of the bioelectric potential (BEP) in the root environment-plants system, biometric and biochemical parameters of plant production using various nutrient solutions during the cultivation of lettuce by the panoponic method in intensive artificial lightculture have been registered. The average BEP value was 243 mV when using the nitrate form of nitrogen in the nutrient solution, 147 mV for the amide form and 178 mV for the nitrate-ammonium form. The maximum observed potential difference was characteristic of the variant containing nitrogen in the nitrate form, which probably indicates the potential-forming role of NO3-. An increase in the concentration of positive ions (Ca2+, K+, NH4+) on the lower electrode and negative (NO3-) on the upper one was noted, which corresponds to the polarity observed in the BES – the upper electrode is electronegative relative to the lower one. It is shown that the nutrient solution containing nitrogen in the nitrate form was the best in terms of size and stability of the studied options. The diffusion of ions in the root environment during the life of plants can become a new alternative source of electricity.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115700315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MITOCHONDRIA-TARGETED ANTIOXIDANT SKQ1 REGULATES OF SIGNAL SYSTEM KEAP1/NRF2/ARE AND APOPTOSIS IN THE LEUKOCYTES OF BLOOD UNDER OXIDATIVE STRESS 线粒体靶向抗氧化剂skq1调控氧化应激下血液白细胞的信号系统keap1 / nrf2 / are和凋亡

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-24 DOI: 10.29039/rusjbpc.2022.0569

O. Gutsenko, I. Kornienko, A. Ananyan, N. Milutina, V. Vnukov

{"title":"MITOCHONDRIA-TARGETED ANTIOXIDANT SKQ1 REGULATES OF SIGNAL SYSTEM KEAP1/NRF2/ARE AND APOPTOSIS IN THE LEUKOCYTES OF BLOOD UNDER OXIDATIVE STRESS","authors":"O. Gutsenko, I. Kornienko, A. Ananyan, N. Milutina, V. Vnukov","doi":"10.29039/rusjbpc.2022.0569","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0569","url":null,"abstract":"This study demonstrated that hyperoxia induced (0.5 MPa, 90 min) oxidative stress decreased mRNA level of Nrf2 transcription factor and Nrf2 induced genes encoding antioxidant enzymes (SOD1, CAT, GPx4) in leukocytes of rat blood. The change in gene expression profiles in hyperoxia was accompanied by disbalance of antioxidant enzyme activities in the leukocytes – activation of superoxide dismutase, and inhibition of catalase, glutathione peroxidase. The administration of SkQ1 (50 nmol/kg during 5 days) significantly increased mRNA level of Nrf2 transcription factor and Nrf2 induced genes encoding antioxidant enzymes SOD2 and GPx4 and nor malized the transcriptional activity of the SOD1 and CAT genes in the leukocytes under the hyperoxia induced oxidative stress. Catalase and glutathione peroxidase activity in the leukocytes increased concurrently with reversion of superoxide dismutase activity to the control level. The protective effect of SkQ1 in hyperoxia induced oxidative stress may be realized via direct antioxidant activity and the stimulation of the Keap1/Nrf2/ARE defense system. Administration of SkQ1 in extremely low amounts (50 nmol/kg for 5 days) effectively inhibits the accumulation of lipid peroxidation molecular products, normalizes the structural state of lymphocyte membranes and the level of their apoptosis under the condition of physiological standard and oxidative stress.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124008349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LYMPHOCYTES APOPTOSIS UNDER THE ACTION OF PHYSICAL FACTORS COMBINATION 物理因素联合作用下淋巴细胞凋亡

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0524

E. Nikanorova, I. Varganova, Y. Medvedev, V. Nagiba

{"title":"LYMPHOCYTES APOPTOSIS UNDER THE ACTION OF PHYSICAL FACTORS COMBINATION","authors":"E. Nikanorova, I. Varganova, Y. Medvedev, V. Nagiba","doi":"10.29039/rusjbpc.2022.0524","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0524","url":null,"abstract":"The article presents the comparative study results of the bioeffects of 30-minute exposure session to electromagnetic radiation with 1000 MHz frequency and 10 mW/cm2 EFD; of 30-minute exposure session to low-frequency pulsed magnetic field with 4 mT magnetic induction, as well as their combination, on rat’s lymphocytes apoptosis level in vivo. The apoptosis level was assessed morphologically with fluorescent microscope after 24-hours of cell suspension cultivation at immunological plates at 37 0C in nutrient medium without loading. The percentage of cells with morphological signs of apoptosis (margination, condensation and fragmentation of chromatin in nucleus) was quantitatively determined. It was found that electromagnetic irradiation had no significant impact on apoptosis level, but magnetic field action resulted in reducing the apoptosis cells number by 16% (p≤0,01) compared to the control group. The combination of electromagnetic radiation and low intensity magnetic field increased the apoptotic cells number by 28% (p ≤0.01) compared to the control. Thus, the mediated effect of electromagnetic radiation on blood cells genome leads to disruption of its normal functioning. It has been shown that low-frequency magnetic field can influence on intracellular processes in different directions; in combination with electromagnetic radiation it can intensify pathological reactions, and thus launching the apoptosis massive program.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134178029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE EFFECTS OF COBRA CARDIOTOXINS ON THE PAPILLARY MUSCLE AND LANGENDORF PERFUSED RAT HEART ARE NOT ASSOCIATED WITH ADRENALINE RELEASE 眼镜蛇心脏毒素对大鼠乳头肌和兰根多夫灌注心脏的影响与肾上腺素释放无关

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0515

A. Averin, F. Tyurin, K. Samodurova, V. Starkov, T. Andreeva, Y. Utkin

{"title":"THE EFFECTS OF COBRA CARDIOTOXINS ON THE PAPILLARY MUSCLE AND LANGENDORF PERFUSED RAT HEART ARE NOT ASSOCIATED WITH ADRENALINE RELEASE","authors":"A. Averin, F. Tyurin, K. Samodurova, V. Starkov, T. Andreeva, Y. Utkin","doi":"10.29039/rusjbpc.2022.0515","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0515","url":null,"abstract":"In a study of the effects of cobra cardiotoxins on myocardial tissue, both right ventricular (papillary muscle) and left ventricular contractility (isovolumic recording of left ventricular pulse pressure during Langendorff perfusion of the heart) were assessed. In papillary muscle, both toxins, at a concentration of 5 μg/mL, caused short-term increases in contractility to 200±25% and 171±15% for CTX-1 and CTX-2, respectively, at the point of maximum effect. At the same time, for CTX-1 and CTX-2, the time to peak tension (TPT) increased from 104±2 to 111±2 and from 96±2 to 104±5 ms, the relaxation time to 50%(TR50%) from 64±4 to 70±6 and from 64±6 to 69±7 ms, and the relaxation time to 95%(TR95%) from 163±10 to 190±22 and from 148±16 to 155±20 ms, respectively. This significantly differs from the positive inotropic effect of the β-adrenomimetic isoproterenol (170±31%), which causes acceleration of TPT from 106±5 to 89±4 ms, TR50% from 58±6 to 43±4 ms, and TR95% from 145±15 to 90±14 ms. When the whole heart was exposed to cardiotoxins, an increase in contractility was also observed, followed by its suppression and contracture, in contrast to isoproterenol, which caused a steady increase in contractility coupled with an increase in heart rate. Pretreatment of papillary muscles with the β-blocker propranolol \u0000(10 μM) did not prevent the development of cardiotoxin effects, but completely blocked the effects of isoproterenol. Our data indicate that the temporary increase in contractility under the action of cardiotoxins is not associated with the release of endogenous adrenaline, but rather is caused by changes in calcium homeostasis in cardiomyocytes.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134405780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EFFECT OF COBALT FERRITE MAGNETIC NANOPARTICLES IN HYDROPHILIC COATING ON THE CONDUCTIVITY OF BILAYER LIPID MEMBRANES 亲水涂层中钴铁氧体磁性纳米颗粒对双层脂膜电导率的影响

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0501

E. Borisova, B. Kozhemova, O. Konstantinov, E. Korepanova, V. Mikheev, A. Mikhnich, V. Sukhova, P. Astanina, O. Koplak, A. Anosov

{"title":"EFFECT OF COBALT FERRITE MAGNETIC NANOPARTICLES IN HYDROPHILIC COATING ON THE CONDUCTIVITY OF BILAYER LIPID MEMBRANES","authors":"E. Borisova, B. Kozhemova, O. Konstantinov, E. Korepanova, V. Mikheev, A. Mikhnich, V. Sukhova, P. Astanina, O. Koplak, A. Anosov","doi":"10.29039/rusjbpc.2022.0501","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0501","url":null,"abstract":"Magnetic nanoparticles are widely used in medical applications, for example, as contrast agents for magnetic resonance imaging, to enhance hyperthermia, for targeted drug delivery, theranostics (therapy + diagnostics). When used in vivo, magnetic nanoparticles require a special coating that promotes biocompatibility and minimizes potential toxicity under physiological conditions. Human serum albumin or polyethylene glycol can be used as a coating. In this work, the interactions of magnetic cobalt ferrite nanoparticles (12 and 27 nm size) with diphitanoylphosphatidylcholine bilayer lipid membranes were investigated. The conductivity of membranes varied over a wide range – two groups of membranes can be distinguished – membranes, the conductivity of which (after the addition of nanoparticles) remained practically unchanged compared to the control, and membranes, the conductivity of which, upon the addition of nanoparticles, changed abruptly and increased in a wide range by 0.5–2 orders of magnitude. Significant differences between the conductivities upon the addition of the same volume of 12 and 27 nm nanoparticles surrounded by human serum albumin were not found. When adding nanoparticles with a coating of polyethylene glycol, the conductivity increased much weaker than when adding nanoparticles with a coating of human serum albumin. The results suggest that hydrophilic coated magnetic nanoparticles interact with the membranes, which can lead to the appearance of metastable conducting pores, which, in turn, increase the integral conductivity of the membranes.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"59 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120961433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0