Russian Journal of Biological Physics and Chemisrty最新文献

{"title":"THE ROLE OF NITRIC OXIDE UNDER THE INFLUENCE OF UV-B RADIATION ON YEAST CELLS","authors":"N. Kocharli, S. Hummatova","doi":"10.29039/rusjbpc.2023.0581","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0581","url":null,"abstract":". The present work is devoted to the study of nitric oxide production in yeast cells after exposure to UV-B radiation. In addition, a comparative evaluation of the production of reactive oxygen species (ROS) was carried out. The change in the level of ROS was determined using the fluorescent dye \u00002',7'-dichlorodihydrofluorescein diacetate-H2DCF•DA. That dye is used to determine the level of ROS in living cells. We determined the intracellular concentration of nitric oxide using a fluorescent dye; -4-amino-5-methylamino-2',7'-difluorescein diacetate (DAF-FM). It was found that under the action of UV-B radiation on cells, depending on the dose, the rate of oxidation of the dye 2',7'-dichlorodihydrofluorescein diacetate (H2DCF•DA) increases and a high intensity of DCF fluorescence is observed. Under the action of a high dose (4,8∙102 erg/mm2.) of UV-B rays, the generation of ROS and nitric oxide decreases. As can be seen on the obtained data, at high doses of radiation, the intensity of chemiluminescence of lucigenin remains at a high level.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"115 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140967986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PLANKTON FRACTIONATION BY SEQUENTIAL FILTRATION AND CONSTRUCTION OF BIODIVERSITY PYRAMIDS","authors":"M. Savitsky, A. Kuznetsov","doi":"10.29039/rusjbpc.2023.0596","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0596","url":null,"abstract":"Lotka-Volterra predator-prey models are used to study community ecology, but their ability to generate ecological pyramids compared to field data has not been investigated in detail. In this paper, agent-based modeling (ABM) was used instead of systems of ordinary differential equations (ODE). It was shown that the two-component producer-consumer system is unstable, whereas the three-component system with consumers of the 1st and 2nd order is stable under prolonged simulation. Time slices as the program progresses can generate both ecological pyramids and cascades. Simulation results are consistent with experiments on separation of the Black Sea plankton from the area of Cape Fiolent (Crimea) into fractions ranging in size from 2 mm to 2 microns. Although biodiversity in individual samples at different points in time as well as abundance vary widely, both predictably decline with rising trophic levels in cases where the number of tests increases over time.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"22 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140967276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF UV RADIATION AND TEMPERATURE ON PAPAIN ACTIVITY","authors":"V. Koroleva, S. Pankova, Yu. Redko, M. Holyavka, V. Artyukhov","doi":"10.29039/rusjbpc.2023.0589","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0589","url":null,"abstract":"Papain (EC 3.4.22.2) is an endolytic cysteine protease derived from papaya latex (Carica papaya L.). Today, this enzyme is considered as an anti-inflammatory, anticoagulant and hemolytic agent, as well as a substance that accelerates tissue repair. Chitosan is classified as a universal sorbent that binds a wide range of substances of organic and inorganic origin. It has antimicrobial activity, biodegradability, non-toxicity and bioadhesive properties. The aim of our work is to study the combined effect of UV-radiation and temperature on the activity of free papain and papain immobilized on a matrix of medium and high molecular weight chitosan. Papain was chosen as the object of study, bovine serum albumin (BSA) and azocasein served as substrates for hydrolysis, medium- (Mr = 200 kDa, degree of deacetylation (DD) 82%) and high molecular weight (Mr = 350 kDa, DD = 94.85%) chitosan were used as carriers for immobilization. It has been shown that the enzyme becomes more resistant to changes in the temperature regime, as well as to UV irradiation after its immobilization. At 90°C, native papain is completely inactivated, while the sorbed enzyme on medium and high molecular weight chitosan retains 45 and 57% of its initial activity, respectively. After the sorption of papain on the polysaccharide matrix at doses from 151 to 6040 J/m2, the catalytic ability of the enzyme changed to a lesser extent than that of its soluble form. The proteolytic ability of the biocatalyst immobilized on high molecular weight chitosan after UV irradiation even increased by 13-27% of the initial level.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"32 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABOUT INFORMATIONAL OPPORTUNITIES AND RESULTS OF EXPERIMENTAL STUDIES OF GDV VISUALIZATION PROTEIN-INDUCED MOLECULAR COMPLEXES IN AQUEOUS SOLUTIONS OF TABLETS, CONTAINING ANTIBODIES TO INTERFERON-γ","authors":"O. Maslennikova, A. Sibirev, M. Shipko, M. Stepovich","doi":"10.29039/rusjbpc.2023.0593","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0593","url":null,"abstract":"The character of changes in the positional order in the arrangement of protein-induced water associates in aqueous solutions of tableted drugs containing antibodies to interferon-γ was studied by the method of gas-discharge visualization. As objects of study, we used aqueous solutions of tablet forms, which were obtained by applying antibody solutions (with a concentration of 10–24 mg/ml) onto a carrier (89% raw lactose, 10% microcrystalline cellulose, 1% magnesium stearate). The informative possibilities of the gas-discharge visualization method in the analysis of data on the entropy, fractality, and length of emission isolines caused by kilovolt electrons released from aqueous solutions of tablet preparations containing antibodies to interferon-γ are considered. It has been shown that the activation of solutions by antibodies to interferon-γ is associated with the intensification of associative processes accompanied by the ordering of protein-induced molecular complexes. In general, the obtained results indicate the preservation of water associates formed in the process of ultrahigh dilution in solutions of tableted samples with antibodies to interferon.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"26 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140970990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ON THE SUBSTRATE SPECIFICITY AND SOME PROPERTIES OF THE EXTRACELLULAR OXIDASE FROM THE NEONOTHOPANUS NAMBI BASIDIOMYCETE","authors":"O. Mogilnaya, N. Ronzhin, E. Posokhina, V. Bondar","doi":"10.29039/rusjbpc.2023.0591","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0591","url":null,"abstract":"An extracellular enzyme with oxidase activity was isolated from the mycelium of the higher fungus Neonothopanus nambi by mild treatment of the biomass with β-glucosidase. A substrate specificity and some properties of the isolated extracellular oxidase were studied in the present work. Experiments revealed that the extracellular oxidase exhibited activity with most phenolic compounds chosen as model substrates. It is important to note that the enzyme exhibited a catalytic function in the reactions without the addition of exogenous hydrogen peroxide and other mediators. The highest catalytic activity of the enzyme was observed with veratryl alcohol and dihydric phenols, hydroquinone and guaiacol. The enzyme showed lower activity with aromatic azo compounds (ABTS, DAB, o-dianisidine). In reactions with dihydric phenol resorcinol and monophenol, the enzyme efficiency was extremely low. The kinetic parameters of the enzymatic reactions with actively oxidized substrates were determined. The addition of a divalent metal ion chelator (EDTA) did not affect the activity of the enzyme, while the addition of the SH reagent (DTT) increased the catalytic efficiency of the studied oxidase. The totality of the data obtained indicates that the extracellular oxidase of the N. nambi fungus catalyzes the oxidation of a wide range of aromatic compounds under slightly acidic and neutral conditions without the addition of additional mediators (in particular, hydrogen peroxide). This creates the prerequisites for studying the applicability of the enzyme in biomedical analytics.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"26 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140968673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ELECTRONIC PARAMETERS OF CONFORMATIONAL STATES OF ABETA-AMYLOID PEPTIDE (25-35)","authors":"G. Agaeva, G. Najafova","doi":"10.29039/rusjbpc.2023.0583","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0583","url":null,"abstract":"The electronic characteristics of stable conformational states of beta-amyloid peptide (25-35) have been studied by molecular modeling methods. Beta-amyloid peptide (Aβ) is the main component of senile plaques found in the brains of patients with Alzheimer's disease. Aβ (25-35) fragment of a molecule with the amino acid sequence Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met. It has been shown that the conformational features of a molecule are largely determined by its environment; therefore, the aim of this work was to study the differences in the conformations of amyloid beta-peptide (25-35) in vacuum and in a low-polarity medium using the methods of molecular mechanics and quantum chemical calculations. The studies were carried out in two stages: 1) the geometric and energy parameters of the low-energy conformational states of the indicated peptide were determined, 2) the electronic characteristics of the established low-energy conformations of these molecule were calculated. Calculations showed that the conformational behavior of this peptide can be described by a set of similar low-energy conformations with structure on the C-terminal sequence. It has also been shown that the low-energy structures of the amyloid beta-peptide (25-35) have the most favorable dispersion contacts and, therefore, it can be expected that they will become most preferable in a low-polarity environment, when electrostatic interactions do not play a significant role. Permissible changes in the dihedral angles of the amyloid beta-peptide (25-35) in the energetically preferred conformation and the stability of secondary structure elements were also investigated. The distribution of electron density and equipotential surfaces in the energetically preferred conformations of the beta-amyloid peptide were obtained and compared (25-35). Quantum chemical calculations showed that the largest negative charges in the peptide are concentrated precisely on the oxygen atoms of the carbonyl groups and the nitrogen atoms of the amino group. The distribution of electron density and equipotential surfaces in the energetically preferred conformations of the beta-amyloid peptide (25-35) were obtained and compared.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"12 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140967188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STRUCTURAL ORGANIZATION OF LACTOFERROXIN MOLECULES","authors":"L. Agayeva, A. Abdinova, S. Akhmedova, N. Akhmedov","doi":"10.29039/rusjbpc.2023.0585","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0585","url":null,"abstract":"The conformational possibilities of lactoferroxin A (H-Tyr1-Leu2-Gly3-Ser4-Gly5-Tyr6-OH) and lactoferroxin B (H-Arg1-Tyr2-Tyr3-Gly4-Tyr5-OH) molecules were studied by theoretical conformational analysis. The potential energy of the system is chosen as the sum of non-valence, electrostatic and torsion interactions and the energy of hydrogen bonds. The low-energy conformations of molecules, the dihedral angles of the main and side chains of amino acid residues that make up the molecule were found, and the energy of intra- and interresidual interactions was estimated. It is shown that the spatial structure of the lactoferroxin A molecule is represented by fourteen forms of the main chain, and the spatial structure of the lactoferroxin B molecule by eleven forms of the main chain. Comparison of the obtained low-energy conformations of lactoferroxins A and B shows that they have quite a lot in common. In a small energy range of 0–3,0 kcal/mol, these molecules have many conformations. Therefore, it is precisely this that explains the fact that both molecules perform a common biological function. It can be assumed that tyrosine amino acid residues are involved in the performance of the biological function. In similar conformations, their side chains in space are approximately in the same positions. The results obtained can be used to elucidate the structural and structural-functional organization of lactoferroxin molecules.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"21 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140967425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIGMA-1 RECEPTOR ANTAGONIST, COMPOUND BD-1063, ATTENUATES Ca2+ RESPONSES INDUCED BY MOLIXAN IN MACROPHAGES","authors":"L. Milenina, Z. Krutetskaya, V. Antonov, N. Krutetskaya, V. Badulina, A. Simonyan","doi":"10.29039/rusjbpc.2023.0588","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0588","url":null,"abstract":"Sigma-1 receptors are ubiquitous multifunctional ligand-operated molecular chaperones in the endoplasmic reticulum membrane with a unique history, structure, and pharmacological profile. Acting as chaperones, sigma-1 receptors modulate a wide range of cellular processes in health and disease, including Ca2+ signaling processes. The pharmacological analogue of oxidized glutathione, drug molixan®, is used as an immunomodulator and cytoprotector in the complex therapy of bacterial, viral and oncological diseases; effective in the prevention and treatment of coronavirus infection COVID-19. To elucidate the involvement of sigma-1 receptors in the effect of molixan on the intracellular Ca2+ concentration in macrophages and in the regulation of Ca2+ signaling processes in macrophages in general, the effect of the sigma-1 receptor selective antagonist, compound BD-1063, on Ca2+ responses induced by molixan in rat peritoneal macrophages was investigated. Using Fura-2AM microfluorimetry we have shown for the first time that compound BD-1063 significantly suppresses both Ca2+ mobilization from intracellular Ca2+ stores and subsequent store-dependent Ca2+ entry, induced by molixan in peritoneal macrophages. The data obtained indicate the involvement of sigma-1 receptors in the complex signaling cascade triggered by molixan and leading to intracellular Ca2+ concentration increase in macrophages. The results also suggest the involvement of sigma-1 receptors in the regulation of store-dependent Ca2+ entry in macrophages.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"120 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140967871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE GELATINOUS-TO-FORAGE ZOOPLANKTON BIOMASS RATIO OF THE WORLD OCEAN","authors":"Sergey Piontkovskiy, I. Minsky, Ya. V. Meger","doi":"10.29039/rusjbpc.2023.0595","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0595","url":null,"abstract":"The spatiotemporal variability of ocean profound biophysical characteristics (i.e. the intensity of bioluminescence, soun scattering layers and etc.) gradually depends on the zooplankton biomass distribution pattern. In turn, the forage zooplankton fraction (which is represented basically by crustaceans contributing to a small pelagic fish diet) and the gelatinous zooplankton fraction (which is characterized by jellyfish, ctenophores, and some other organisms), both play important but different roles in matter and energy transfer in the pelagic World Ocean. Forage zooplankton is the transformer that links producers to high trophic-level consumers. The gelatinous zooplankton channels the organic carbon towards a detrital pathway. The ratio of forage-to-gelatinous zooplankton biomass inferred out of international databases (JeDI and COPEPOD) and published papers (with data averaged for 1951-2014), was calculated. The dominance of gelatinous biomass (in carbon units) over the forage biomass in the upper 200 m layer across the World Ocean’s northern hemisphere was shown. This dominance can negatively impact fish stock recruitment.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"18 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140967709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MECHANISM OF ACTION OF THE HEAVY METAL IONS ON THE PHYSICOCHEMICAL PROPERTIES OF PHOSPHOLIPIDS","authors":"L. Shishkina, P. Beletskaya, A. Dubobik, A. Mashukova, V. Shvydkiy","doi":"10.29039/rusjbpc.2023.0597","DOIUrl":"https://doi.org/10.29039/rusjbpc.2023.0597","url":null,"abstract":"Effect of the divalent copper and iron ions on their ability to form complexes with phospholipids and the lipid peroxidation processes intensity, to affect the self-aggregation of the natural lipids in the water medium and ξ-potential of formed particles are studied in the wild range of the metal ion concentrations. Mathematical analysis of UV-spectra of the water solutions of lecithin, metal ions and their mixtures by Gauss method is revealed that copper and iron ions form complexes with phospholipids and act on content of the oxidation products. Besides, the scale of effect depends on the ion concentration, its nature and the lipid oxidizability. The absence of the linear dependence of the ξ-potential value for the lecithin particles on the ion concentration in the water medium and the essential differences of their ability to act on size of formed by lecithin aggregates are obtained. The obtained data allow us to conclude that quality of the natural water is due to both concentrations of the heavy metal ions and their ability to act on the lipid peroxidation regulatory system parameters of the inherent in water bio-objects.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"9 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}