Journal of Radiation Research最新文献_第7页

Inhibition of intracellular ATP synthesis impairs the recruitment of homologous recombination factors after ionizing radiation. 电离辐射后，抑制细胞内 ATP 合成会损害同源重组因子的招募。

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-05-23 DOI: 10.1093/jrr/rrae005

Ryota Hayashi, Hikaru Okumura, Mayu Isono, Motohiro Yamauchi, Daiki Unami, Rahmartani Tania Lusi, Masamichi Yamamoto, Yu Kato, Yuki Uchihara, Atsushi Shibata

{"title":"Inhibition of intracellular ATP synthesis impairs the recruitment of homologous recombination factors after ionizing radiation.","authors":"Ryota Hayashi, Hikaru Okumura, Mayu Isono, Motohiro Yamauchi, Daiki Unami, Rahmartani Tania Lusi, Masamichi Yamamoto, Yu Kato, Yuki Uchihara, Atsushi Shibata","doi":"10.1093/jrr/rrae005","DOIUrl":"10.1093/jrr/rrae005","url":null,"abstract":"Ionizing radiation (IR)-induced double-strand breaks (DSBs) are primarily repaired by non-homologous end joining or homologous recombination (HR) in human cells. DSB repair requires adenosine-5'-triphosphate (ATP) for protein kinase activities in the multiple steps of DSB repair, such as DNA ligation, chromatin remodeling, and DNA damage signaling via protein kinase and ATPase activities. To investigate whether low ATP culture conditions affect the recruitment of repair proteins at DSB sites, IR-induced foci were examined in the presence of ATP synthesis inhibitors. We found that p53 binding protein 1 foci formation was modestly reduced under low ATP conditions after IR, although phosphorylated histone H2AX and mediator of DNA damage checkpoint 1 foci formation were not impaired. Next, we examined the foci formation of breast cancer susceptibility gene I (BRCA1), replication protein A (RPA) and radiation 51 (RAD51), which are HR factors, in G2 phase cells following IR. Interestingly, BRCA1 and RPA foci in the G2 phase were significantly reduced under low ATP conditions compared to that under normal culture conditions. Notably, RAD51 foci were drastically impaired under low ATP conditions. These results suggest that HR does not effectively progress under low ATP conditions; in particular, ATP shortages impair downstream steps in HR, such as RAD51 loading. Taken together, these results suggest that the maintenance of cellular ATP levels is critical for DNA damage response and HR progression after IR.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":" ","pages":"263-271"},"PeriodicalIF":2.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11115441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling of a tissue expander with a radiofrequency identification port in postmastectomy radiation therapy planning. 带射频识别端口的组织扩张器在乳房切除术后放射治疗规划中的建模。

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-05-23 DOI: 10.1093/jrr/rrae004

Fumiyasu Matsubayashi, Taro Takahashi, Hikaru Miyauchi, Yasushi Ito, Arisa Harada, Yasuo Yoshioka

{"title":"Modeling of a tissue expander with a radiofrequency identification port in postmastectomy radiation therapy planning.","authors":"Fumiyasu Matsubayashi, Taro Takahashi, Hikaru Miyauchi, Yasushi Ito, Arisa Harada, Yasuo Yoshioka","doi":"10.1093/jrr/rrae004","DOIUrl":"10.1093/jrr/rrae004","url":null,"abstract":"The purpose of this study was to evaluate the dose attenuation of Motiva Flora® (Flora, Establishment Labs, Alajuela, Costa Rica) tissue expander with a radiofrequency identification port locator and to develop a model for accurate postmastectomy radiation therapy planning. Dose attenuation was measured using an EBT3 film (Ashland, Bridgewater, NJ), and the optimal material and density assignment for the radiofrequency identification coil for dose calculation were investigated using the AcurosXB algorithm on the Eclipse (Varian Medical Systems, Palo Alto, CA) treatment planning system. Additionally, we performed in vivo dosimetry analysis using irradiation tangential to the Flora tissue expander to validate the modeling accuracy. Dose attenuations downstream of the Flora radiofrequency identification coil was 1.29% for a 6 MV X-ray and 0.99% for a 10 MV X-ray when the coil was placed perpendicular to the beam. The most suitable assignments for the material and density of the radiofrequency identification coil were aluminum and 2.27 g/cm3, respectively, even though the coil was actually made of copper. Gamma analysis of in vivo dosimetry with criteria of 3% and 2 mm did not fail in the coil region. Therefore, we conclude that the model is reasonable for clinical use.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":" ","pages":"360-368"},"PeriodicalIF":2.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11115439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustained activation of the FGF1–MEK–ERK pathway inhibits proliferation, invasion and migration and enhances radiosensitivity in mouse angiosarcoma cells 持续激活 FGF1-MEK-ERK 通路可抑制小鼠血管肉瘤细胞的增殖、侵袭和迁移，并增强其放射敏感性

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-04-18 DOI: 10.1093/jrr/rrae021

Taichi Miura, Junko Kado, Kazuma Ashisuke, Mikio Masuzawa, Fumiaki Nakayama

{"title":"Sustained activation of the FGF1–MEK–ERK pathway inhibits proliferation, invasion and migration and enhances radiosensitivity in mouse angiosarcoma cells","authors":"Taichi Miura, Junko Kado, Kazuma Ashisuke, Mikio Masuzawa, Fumiaki Nakayama","doi":"10.1093/jrr/rrae021","DOIUrl":"https://doi.org/10.1093/jrr/rrae021","url":null,"abstract":"Angiosarcoma is a rare refractory soft-tissue tumor with a poor prognosis and is treated by radiotherapy. The fibroblast growth factor 1 (FGF1) mutant, with enhanced thermostability due to several substituted amino acids, inhibits angiosarcoma cell metastasis, yet the mechanism of action is unclear. This study aims to clarify the FGF1 mutant mechanism of action using ISOS-1 mouse angiosarcoma cells. The wild-type FGF1 or FGF1 mutant was added to ISOS-1 cells and cultured, evaluating cell numbers over time. The invasive and migratory capacity of ISOS-1 cells was assessed by transwell analysis. ISOS-1 cell radiosensitivity was assessed by colony formation assay after X-ray irradiation. To examine whether mitogen-activated protein kinase (MEK) inhibitor counteracts the FGF1 mutant effects, a combination of MEK inhibitor and FGF1 mutant was added to ISOS-1 cells and cultured. The FGF1 mutant was observed to inhibit ISOS-1 cell proliferation, invasion and migration by sustained FGF1 signaling activation. A MEK inhibitor suppressed the FGF1 mutant-induced inhibition of proliferation, invasion and migration of ISOS-1 cells. Furthermore, the FGF1 mutant enhanced radiosensitivity of ISOS-1 cells, but MEK inhibition suppressed the increased radiosensitivity. In addition, we found that the FGF1 mutant strongly inhibits actin polymerization, suggesting that actin cytoskeletal dynamics are closely related to ISOS-1 cell radiosensitivity. Overall, this study demonstrated that in ISOS-1 cells, the FGF1 mutant inhibits proliferation, invasion and migration while enhancing radiosensitivity through sustained activation of the MEK-mediated signaling pathway.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":"50 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140623038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are prophylactic antibiotics required for combined intracavitary and interstitial brachytherapy of gynecologic cancers? 妇科癌症联合腔内和间质近距离放射治疗是否需要预防性抗生素？

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-04-13 DOI: 10.1093/jrr/rrae018

Takuya Kumazawa, Yu Ohkubo, Keishiro Mochida, Saori Kondo, Osamu Oguchi, Daisaku Yoshida

{"title":"Are prophylactic antibiotics required for combined intracavitary and interstitial brachytherapy of gynecologic cancers?","authors":"Takuya Kumazawa, Yu Ohkubo, Keishiro Mochida, Saori Kondo, Osamu Oguchi, Daisaku Yoshida","doi":"10.1093/jrr/rrae018","DOIUrl":"https://doi.org/10.1093/jrr/rrae018","url":null,"abstract":"The purpose of this study is to evaluate the need for prophylactic antibiotic treatment prior to combined intracavitary and interstitial (hybrid) brachytherapy for gynecologic cancer. A total of 105 gynecologic cancer patients received 405 brachytherapy sessions, including 302 sessions of intracavitary brachytherapy and 103 sessions of hybrid brachytherapy. Prophylactic antibiotics were administered before 35% of the hybrid brachytherapy sessions. The incidence of postbrachytherapy fever and the frequency of subsequent antibiotic use for infection were compared between treatment groups. Among patients treated with hybrid brachytherapy, fever ≥37.5°C occurred in 16.4% of those not receiving prophylactic antibiotics and 16.7% of those receiving prophylactic antibiotics (P &gt; 0.05). Similarly, fever ≥38.0°C occurred in 4.9% of patients not receiving prophylactic antibiotics and 2.4% of those receiving prophylactic antibiotics (P &gt; 0.05). Additional antibiotics were used to treat postbrachytherapy infections in 4.8% of the group receiving prophylactic antibiotics and 0% of those not receiving prophylactic antibiotics, again without statistically significant difference. There were also no significant differences in posttreatment fever incidence and antibiotics use for infection between intracavitary brachytherapy and hybrid brachytherapy sessions. In conclusion, the incidences of infection and fever are low following hybrid brachytherapy, so prophylactic antibiotics are generally unnecessary.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":"35 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proton beam reirradiation for locally recurrent rectal cancer patients with prior pelvic irradiation 质子束再照射治疗曾接受盆腔照射的局部复发直肠癌患者

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-04-11 DOI: 10.1093/jrr/rrae019

Yoshiaki Takagawa, Motohisa Suzuki, Ichiro Seto, Yusuke Azami, Masanori Machida, Kanako Takayama, Nor Shazrina Sulaiman, Tatsuhiko Nakasato, Yasuhiro Kikuchi, Masao Murakami, Michitaka Honda, Yasushi Teranishi, Koji Kono

{"title":"Proton beam reirradiation for locally recurrent rectal cancer patients with prior pelvic irradiation","authors":"Yoshiaki Takagawa, Motohisa Suzuki, Ichiro Seto, Yusuke Azami, Masanori Machida, Kanako Takayama, Nor Shazrina Sulaiman, Tatsuhiko Nakasato, Yasuhiro Kikuchi, Masao Murakami, Michitaka Honda, Yasushi Teranishi, Koji Kono","doi":"10.1093/jrr/rrae019","DOIUrl":"https://doi.org/10.1093/jrr/rrae019","url":null,"abstract":"The aim of the present study was to report the feasibility of proton beam reirradiation for patients with locally recurrent rectal cancer (LRRC) with prior pelvic irradiation. The study population included patients who were treated with proton beam therapy (PBT) for LRRC between 2008 and December 2019 in our institution. Those who had a history of distant metastases of LRRC, with or without treatment, before reirradiation, were excluded. Overall survival (OS), progression-free survival (PFS) and local control (LC) were estimated using the Kaplan–Meier method. Ten patients were included in the present study. The median follow-up period was 28.7 months, and the median total dose of prior radiotherapy (RT) was 50 Gy (range, 30 Gy–74.8 Gy). The median time from prior RT to reirradiation was 31.5 months (range, 8.1–96.6 months), and the median reirradiation dose was 72 Gy (relative biological effectiveness) (range, 56–77 Gy). The 1-year/2-year OS, PFS and LC rates were 100%/60.0%, 20.0%/10.0% and 70.0%/58.3%, respectively, with a median survival time of 26.0 months. Seven patients developed a Grade 1 acute radiation dermatitis, and no Grade ≥ 2 acute toxicity was recorded. Grade ≥ 3 late toxicity was recorded in only one patient, who had developed a colostomy due to radiation-related intestinal bleeding. Reirradiation using PBT for LRRC patients who had previously undergone pelvic irradiation was feasible. However, the indications for PBT reirradiation for LRRC patients need to be considered carefully due to the risk of severe late GI toxicity.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":"1 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140581063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen-rich solution alleviates acute radiation pneumonitis by regulating oxidative stress and macrophages polarization 富氢溶液通过调节氧化应激和巨噬细胞极化缓解急性放射性肺炎

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-04-08 DOI: 10.1093/jrr/rrae017

Zhen Yin, Wenjing Xu, Junjun Ling, Lihai Ma, Hao Zhang, Pei Wang

{"title":"Hydrogen-rich solution alleviates acute radiation pneumonitis by regulating oxidative stress and macrophages polarization","authors":"Zhen Yin, Wenjing Xu, Junjun Ling, Lihai Ma, Hao Zhang, Pei Wang","doi":"10.1093/jrr/rrae017","DOIUrl":"https://doi.org/10.1093/jrr/rrae017","url":null,"abstract":"This study was aimed to investigate the effect of hydrogen-rich solution (HRS) on acute radiation pneumonitis (ARP) in rats. The ARP model was induced by X-ray irradiation. Histopathological changes were assessed using HE and Masson stains. Inflammatory cytokines were detected by ELISA. Immunohistochemistry and flow cytometry were performed to quantify macrophage (CD68) levels and the M2/M1 ratio. Western blot analysis, RT-qPCR, ELISA and flow cytometry were used to evaluate mitochondrial oxidative stress injury indicators. Immunofluorescence double staining was performed to colocalize CD68/LC3B and p-AMPK-α/CD68. The relative expression of proteins associated with autophagy activation and the adenosine 5′-monophosphate-activated protein kinase/mammalian target of rapamycin/Unc-51-like kinase 1 (AMPK/mTOR/ULK1) signaling pathway were detected by western blotting. ARP decreased body weight, increased the lung coefficient, collagen deposition and macrophage infiltration and promoted M1 polarization in rats. After HRS treatment, pathological damage was alleviated, and M1 polarization was inhibited. Furthermore, HRS treatment reversed the ARP-induced high levels of mitochondrial oxidative stress injury and autophagy inhibition. Importantly, the phosphorylation of AMPK-α was inhibited, the phosphorylation of mTOR and ULK1 was activated in ARP rats and this effect was reversed by HRS treatment. HRS inhibited M1 polarization and alleviated oxidative stress to activate autophagy in ARP rats by regulating the AMPK/mTOR/ULK1 signaling pathway.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":"51 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patterns of care for brachytherapy in Japan. 日本近距离放射治疗的护理模式。

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-03-22 DOI: 10.1093/jrr/rrad099

Hitoshi Ikushima, Noriko Ii, Shin-Ei Noda, Koji Masui, Naoya Murakami, Ken Yoshida, Miho Watanabe, Shinnji Kawamura, Toru Kojima, Yoshihito Nomoto, Takafumi Toita, Tatsuya Ohno, Hideyuki Sakurai, Hiroshi Onishi

{"title":"Patterns of care for brachytherapy in Japan.","authors":"Hitoshi Ikushima, Noriko Ii, Shin-Ei Noda, Koji Masui, Naoya Murakami, Ken Yoshida, Miho Watanabe, Shinnji Kawamura, Toru Kojima, Yoshihito Nomoto, Takafumi Toita, Tatsuya Ohno, Hideyuki Sakurai, Hiroshi Onishi","doi":"10.1093/jrr/rrad099","DOIUrl":"10.1093/jrr/rrad099","url":null,"abstract":"This study aimed to assess the current state of brachytherapy (BT) resources, practices and resident education in Japan. A nationwide survey was undertaken encompassing 177 establishments facilitating BT in 2022. Questionnaires were disseminated to each BT center, and feedback through online channels or postal correspondence was obtained. The questionnaire response rate was 90% (159/177), and every prefecture had a response in at least one center. The number of centers in each prefecture ranged from 0.6 to 3.6 (median: 1.3) per million population. The annual number of patients in each center ranged from 0 to 272 (median: 31). While most prefectures provided intracavitary (IC) BT for gynecological cancers and interstitial (IS) BT for prostate cancer, only one-third of the prefectures provided IS BT for cancer sites other than the prostate. The institutional image-guided BT implementation rate was 71%. IC and IS BT was performed for 15.4% of IC BT cases of gynecological cancer. Only 47% of the BT training centers answered that they could provide adequate training in BT for residents. The most common reason for this finding was the insufficient number of patients in each center. The results show that, although BT has achieved uniformity in terms of facility penetration, new technologies are not yet widespread enough. Furthermore, IS BT, which requires advanced skills, is limited to a few BT centers, and considerable number of BT training centers do not have sufficient caseloads to provide the necessary experience for their residents.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":" ","pages":"168-176"},"PeriodicalIF":2.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10959427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139048935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Japanese nationwide cohort data of proton beam therapy for liver oligometastasis in breast cancer patients. 日本全国范围内质子束治疗乳腺癌患者肝脏寡转移的队列数据。

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-03-22 DOI: 10.1093/jrr/rrad106

Hisashi Yamaguchi, Nobuyoshi Fukumitsu, Haruko Numajiri, Hiroyuki Ogino, Tomoaki Okimoto, Takashi Ogino, Motohisa Suzuki, Shigeyuki Murayama

{"title":"The Japanese nationwide cohort data of proton beam therapy for liver oligometastasis in breast cancer patients.","authors":"Hisashi Yamaguchi, Nobuyoshi Fukumitsu, Haruko Numajiri, Hiroyuki Ogino, Tomoaki Okimoto, Takashi Ogino, Motohisa Suzuki, Shigeyuki Murayama","doi":"10.1093/jrr/rrad106","DOIUrl":"10.1093/jrr/rrad106","url":null,"abstract":"A nationwide multicenter cohort study on particle therapy was launched by the Japanese Society for Radiation Oncology in Japan in May 2016. We analyzed the outcome of proton beam therapy (PBT) for liver oligometastasis in breast cancers. Cases in which PBT was performed at all Japanese proton therapy facilities between May 2016 and February 2019 were enrolled. The patients were selected based on the following criteria: the primary cancer was controlled, liver recurrence without extrahepatic tumors and no more than three liver lesions. Fourteen females, with a median age of 57 years (range, 44-73) and 22 lesions, were included. The median lesion size, fraction (fr) size and biological effective dose were 44 (20-130) mm, 6.6 (2-8) gray (Gy) (relative biological effectiveness)/fr and 109.6 (52.7-115.2) Gy, respectively. The median follow-up period was 22.8 (4-54) months. The 1-, 2- and 3-year local control (LC) rates of liver metastasis from breast cancer were 100% for all. The 1-, 2- and 3-year overall survival rates were 85.7, 62.5 and 62.5%, respectively. The 1-, 2- and 3-year progression-free survival (PFS) rates were 50.0%, 33.3%, and 16.7%, respectively. The median PFS time was 16 months. Only one patient did not complete PBT due to current disease progression. One patient had Grade 3 radiation-induced dermatitis. None of the patients experienced radiation-induced liver failure during the acute or late phase. Owing to the low incidence of adverse events and the high LC rate, PBT appears to be a feasible option for liver oligometastasis in breast cancers.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":" ","pages":"231-237"},"PeriodicalIF":2.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10959435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139697660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-radiation effect of MRN-100: a hydro-ferrate fluid, in vivo. MRN-100 的活体抗辐射效果：一种氢铁酸盐流体。

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-03-22 DOI: 10.1093/jrr/rrad095

Mamdooh Ghoneum, Nariman K Badr El-Din, Mai Alaa El-Dein

{"title":"Anti-radiation effect of MRN-100: a hydro-ferrate fluid, in vivo.","authors":"Mamdooh Ghoneum, Nariman K Badr El-Din, Mai Alaa El-Dein","doi":"10.1093/jrr/rrad095","DOIUrl":"10.1093/jrr/rrad095","url":null,"abstract":"Ionizing radiation (IR) severely harms many organs, especially the hematopoietic tissue, mandating the development of protective nutraceuticals. MRN-100, a hydro-ferrate fluid, has been shown to protect γ-radiated fish against hematopoietic tissue damage and lethality. The current study aimed to examine MRN-100's protective effect against irradiated mice and explore the mechanisms underlying its effect. Mice received a single acute, sub-lethal, 5 Gy, whole body dose of X-ray IR. MRN-100 treatment was administered daily for 2-weeks pre-irradiation until 1-week post-irradiation. Spleen and blood were analysed for oxidative stress, hematological, histological and biochemical parameters. Radiation exposure markedly decreased complete blood count (CBC) parameters including hemoglobin, hematocrit, red blood cells, platelets, white blood cells and lymphocytes, and significantly increased neutrophils. In contrast, MRN-100 supplementation to irradiated mice ameliorated all CBC parameters and protected against DNA damage in both splenic cells and serum. It also had an antioxidant effect, increasing the levels of glutathione, superoxide dismutase, catalase and total antioxidant capacity, which were otherwise decreased by irradiation. MRN-100 intake reduced the oxidative stress biomarker levels of nitric oxide, protein carbonyl, malondialdehyde, reactive oxygen species and 8-hydroxydeoxyguanosine, a marker specific to DNA damage. Furthermore, MRN-100 enhanced serum iron and reversed the radiation-induced elevations of liver enzymes. Finally, MRN-100 protected splenic tissue from irradiation as observed by histology. We conclude that MRN-100 consumption may protect against oxidative stress generated by radiation exposure, suggesting that it may be employed as an adjuvant treatment to prevent radiation's severe damage to important organs.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":" ","pages":"145-158"},"PeriodicalIF":2.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10959437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139512830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiological imaging protection: a study on imaging dose used while planning computed tomography for external radiotherapy in Japan. 放射成像保护：日本外放射治疗计算机断层扫描规划时使用的成像剂量研究。

IF 2 4区医学

Journal of Radiation Research Pub Date : 2024-03-22 DOI: 10.1093/jrr/rrad098

Satoshi Kito, Yuhi Suda, Satoshi Tanabe, Takeshi Takizawa, Tomomasa Nagahata, Naoki Tohyama, Hiroyuki Okamoto, Takumi Kodama, Yukio Fujita, Hisayuki Miyashita, Kazuya Shinoda, Masahiko Kurooka, Hidetoshi Shimizu, Takeshi Ohno, Masataka Sakamoto

{"title":"Radiological imaging protection: a study on imaging dose used while planning computed tomography for external radiotherapy in Japan.","authors":"Satoshi Kito, Yuhi Suda, Satoshi Tanabe, Takeshi Takizawa, Tomomasa Nagahata, Naoki Tohyama, Hiroyuki Okamoto, Takumi Kodama, Yukio Fujita, Hisayuki Miyashita, Kazuya Shinoda, Masahiko Kurooka, Hidetoshi Shimizu, Takeshi Ohno, Masataka Sakamoto","doi":"10.1093/jrr/rrad098","DOIUrl":"10.1093/jrr/rrad098","url":null,"abstract":"Previous studies have primarily focused on quality of imaging in radiotherapy planning computed tomography (RTCT), with few investigations on imaging doses. To our knowledge, this is the first study aimed to investigate the imaging dose in RTCT to determine baseline data for establishing national diagnostic reference levels (DRLs) in Japanese institutions. A survey questionnaire was sent to domestic RT institutions between 10 October and 16 December 2021. The questionnaire items were volume computed tomography dose index (CTDIvol), dose-length product (DLP), and acquisition parameters, including use of auto exposure image control (AEC) or image-improving reconstruction option (IIRO) for brain stereotactic irradiation (brain STI), head and neck (HN) intensity-modulated radiotherapy (IMRT), lung stereotactic body radiotherapy (lung SBRT), breast-conserving radiotherapy (breast RT), and prostate IMRT protocols. Details on the use of motion-management techniques for lung SBRT were collected. Consequently, we collected 328 responses. The 75th percentiles of CTDIvol were 92, 33, 86, 23, and 32 mGy and those of DLP were 2805, 1301, 2416, 930, and 1158 mGy·cm for brain STI, HN IMRT, lung SBRT, breast RT, and prostate IMRT, respectively. CTDIvol and DLP values in institutions that used AEC or IIRO were lower than those without use for almost all sites. The 75th percentiles of DLP in each treatment technique for lung SBRT were 2541, 2034, 2336, and 2730 mGy·cm for free breathing, breath holding, gating technique, and real-time tumor tracking technique, respectively. Our data will help in establishing DRLs for RTCT protocols, thus reducing imaging doses in Japan.","PeriodicalId":16922,"journal":{"name":"Journal of Radiation Research","volume":" ","pages":"159-167"},"PeriodicalIF":2.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10959444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139048946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0