Evolutionary Applications最新文献

{"title":"Signatures of Selection for Resistance/Tolerance to Perkinsus olseni in Grooved Carpet Shell Clam (Ruditapes decussatus) Using a Population Genomics Approach","authors":"Inés M. Sambade,&nbsp;João Estêvão,&nbsp;Marina Pampín,&nbsp;Andreia Cruz,&nbsp;Eric Guévélou,&nbsp;Andrés Blanco,&nbsp;Francisco Câmara,&nbsp;Jessica Gómez-Garrido,&nbsp;Fernando Cruz,&nbsp;Luca Bargelloni,&nbsp;Stefano Carboni,&nbsp;Tyler Alioto,&nbsp;Benjamin Costas,&nbsp;Sergio Fernández-Boo,&nbsp;Paulino Martínez","doi":"10.1111/eva.70106","DOIUrl":"https://doi.org/10.1111/eva.70106","url":null,"abstract":"<p>The grooved carpet shell clam (<i>Ruditapes decussatus</i>) is a bivalve of high commercial value distributed throughout the European coast. Its production has suffered a decline caused by different factors, especially by the parasite <i>Perkinsus olsenii</i>. Improving production of <i>R</i>. <i>decussatus</i> requires genomic resources to ascertain the genetic factors underlying resistance/tolerance to <i>P. olsenii</i>. In this study, the first reference genome of <i>R</i>. <i>decussatus</i> was assembled through long- and short-read sequencing (1677 contigs; 1.386 Mb) and further scaffolded at chromosome level with Hi-C (19 superscaffolds; 95.4% of assembly). Repetitive elements were identified (32%) and masked for annotation of 38,276 coding- and 13,056 non-coding genes. This genome was used as a reference to develop a 2bRAD-Seq 13,438 SNP panel for a genomic screening on six shellfish beds distributed across the Atlantic Ocean and Mediterranean Sea. Beds were selected by perkinsosis prevalence and the infection level was individually evaluated in all the samples. Genetic diversity was significantly higher in the Mediterranean than in the Atlantic region. The main genetic breakage was detected between those regions (F_ST = 0.224), being the Mediterranean more heterogeneous than the Atlantic. Several loci under divergent selection (394 outliers; 261 genomic windows) were detected across shellfish beds. Samples were also inspected to detect signals of selection for resistance/tolerance to <i>P. olsenii</i> by using infection-level and population-genomics approaches, and 90 common divergent outliers for resistance/tolerance to perkinsosis were identified and used for gene mining. Candidate genes and markers identified provide invaluable information for controlling perkinsosis and for improving production of the grooved carpet shell clam.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143939315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Beyond Karstification Have Shaped the Population Structure of a Surface-Dwelling Minnow (Phoxinus lumaireul) Able to Disperse Underground","authors":"Susanne Reier,&nbsp;Peter Trontelj,&nbsp;Luise Kruckenhauser,&nbsp;Martin Kapun,&nbsp;Alexandra Wanka,&nbsp;Anja Palandačić","doi":"10.1111/eva.70104","DOIUrl":"https://doi.org/10.1111/eva.70104","url":null,"abstract":"<p>The Dinaric Karst, a biodiversity hotspot, features complex surface and subterranean hydrological networks that influence aquatic species distribution. This study investigates how karst hydrology shapes the genetic structure of the surface-dwelling minnow <i>Phoxinus lumaireul</i>, examining both large-scale and small-scale population patterns. Using mitochondrial DNA and genome-wide single nucleotide polymorphism (SNP) data of 827 specimens of <i>P. lumaireul</i>, three hypotheses were tested: (1) karst underground water connections facilitate genetic connectivity within and across river systems, whereas non-karst rivers exhibit genetic connectivity mostly within the same system; (2) historical and occasional hydrological connections have shaped present-day population structure, leaving genetic signatures of relatedness where no contemporary hydrological links exist; and (3) genomic approaches provide additional insights into biologically relevant connections that may not be captured by classical tracing tests. The large-scale analyses confirmed three main genetic groups (1a–c), whose structure was likely shaped by Pleistocene glaciations and associated microrefugia rather than by karst hydrology. Small-scale structure analyses revealed that while karst hydrology facilitated gene flow within specific areas, connectivity was uneven and influenced by local hydrological dynamics and historical admixture events. Furthermore, some underground pathways identified by classical tracing tests lacked evidence of genetic connectivity, underscoring the limitations of traditional methods and the added value of genomic data in indirectly detecting biologically relevant hydrological connections. These findings highlight the influence of both historical processes and contemporary karst hydrology on <i>P. lumaireul</i> populations, emphasizing their vulnerability in karst ecosystems and the need for targeted conservation efforts.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting Patterns of Raccoon (Procyon lotor) Spatial Population Genomics Throughout a Rabies Management Area in Eastern North America","authors":"Matthew W. Hopken,&nbsp;Clara P. Mankowski,&nbsp;Christine Thurber,&nbsp;Antoinette J. Piaggio,&nbsp;Kathleen M. Nelson,&nbsp;Richard B. Chipman,&nbsp;Zaid Abdo,&nbsp;Tore Buchanan,&nbsp;Ariane Massé,&nbsp;Amy T. Gilbert","doi":"10.1111/eva.70105","DOIUrl":"https://doi.org/10.1111/eva.70105","url":null,"abstract":"<p>Wide-ranging, generalist species provide both interesting and challenging opportunities for research questions focused on population structure. Their continuous distributions and ability to occupy diverse habitat types can obscure genetic signals of ancestry and geographic clustering. However, spatially informed population genetic approaches are notable for high-resolution identification of geographic clusters that often elude more classical clustering models. The northern raccoon (<i>Procyon lotor</i>) is a broadly distributed species in North America, with populations in diverse habitats ranging from dense urban to rural landscapes. Wildlife management agencies have an interest in understanding raccoon ecology, given their propensity for human-wildlife conflicts and zoonotic diseases. We combined samples from an extensive raccoon tissue repository with a RADcapture panel of 1000 microhaplotype loci to conduct spatial genetic analyses of raccoon populations in eastern North America. Our objective was to estimate patterns of genetic diversity on the landscape that may inform raccoon rabies management. Bayesian clustering analyses delineated multiple ancestry clusters that encompassed large areas across 22 US states and 2 Canadian provinces. We discovered a potential phylogeographic split between central and southern samples from those in the northeast region, which correlates with post-Pleistocene recolonization detected in a multitude of species from the region. A finer scale structure was identified using spatially explicit analyses and demonstrated variable dispersal/gene flow patterns within specific regions. The Appalachian Mountain region restricted local connectivity among raccoons, while raccoon populations in central New York, the Ohio River Valley, southern Québec, and southern Alabama demonstrated high genetic connectivity. The results from this study highlight how raccoon ecology and historical biogeography can help contextualize contrasting hypotheses about the influence of landscape on raccoon movement patterns, which can inform management of zoonotic disease risks at regional scales.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Identification of Lamprey Genera and Anadromous Ecotypes in Watersheds of the Northeastern Pacific Ocean","authors":"G. S. Silver,&nbsp;R. T. Lampman,&nbsp;N. Percival,&nbsp;N. Timoshevskaya,&nbsp;J. J. Smith,&nbsp;K. T. Bentley,&nbsp;J. Wade,&nbsp;S. R. Narum,&nbsp;J. E. Hess","doi":"10.1111/eva.70108","DOIUrl":"https://doi.org/10.1111/eva.70108","url":null,"abstract":"<p>Nonparasitic, nonmigratory Western Brook Lamprey (WBL; <i>Lampetra ayresii</i>), and parasitic, anadromous Western River Lamprey (WRL; <i>L. ayresii</i>) are sympatric lampreys that likely represent different life history variations of a single species. Novel genetic tools are critical for differentiating WBL and WRL, whose larvae preclude morphological identification (ID) and will enable comprehensive assessment of imperiled native lampreys of the Northeastern Pacific (including WBL, WRL, and Pacific Lamprey, <i>Entosphenus tridentatus</i>). We developed 47 candidate single nucleotide polymorphism (SNP) markers using whole genome resequencing of WBL (<i>N</i> = 24) and WRL (<i>N</i> = 15) from Ksi Ts'oohl Ts'ap Creek (Nass River, British Columbia, Canada) which are likely ecotypes distinguished by few divergent SNPs across multiple chromosomes. We used five novel candidate SNPs to perform genetic ID of WBL and WRL ecotypes in collections of mixed native lampreys from lower Columbia River tributaries (<i>N</i> = 1474), Ksi Ts'oohl Ts'ap Creek (<i>N</i> = 352), and ocean phase WRL from the Georgia Basin (Salish Sea, British Columbia, Canada; <i>N</i> = 91). Two previously published SNPs were used to ID genera, <i>Entosphenus</i> versus <i>Lampetra</i>. Morphological ID utilized photographs collected from a subset of genotyped lampreys, and high concordance was demonstrated between ID methods for genera (99%) and <i>Lampetra</i> ecotypes (&gt; 98%). We characterized spatial and temporal composition of lamprey genera and ecotypes surveyed across NE Pacific tributaries under the expectation these compositions would be similar across nearby sites and across years at the same site. Proportions of lamprey genera were highly variable within regions and across years; however, <i>Lampetra</i> ecotypic proportions were spatially and temporally stable. WRL were rare in lower Columbia tributaries (~1% average rate among <i>Lampetra</i>) and common further north (&gt; 40% of <i>Lampetra</i>). Genetic ID methods are powerful monitoring tools that create the novel ability to ascertain genera and ecotypes regardless of life stage, while increasing the efficiency of surveys by eliminating time-intensive morphological data collection.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mammalian Browsers Disrupt Eco-Evolutionary Dynamics in a Forest Tree Restoration Planting","authors":"João Costa e Silva,&nbsp;Brad M. Potts,&nbsp;Peter A. Harrison","doi":"10.1111/eva.70099","DOIUrl":"https://doi.org/10.1111/eva.70099","url":null,"abstract":"<p>Native and restored forests are increasingly impacted by pests and diseases, including large herbivores. While community- and species-level impacts of these tree enemies are often well-documented, there is little understanding of their influence on finer-scale eco-evolutionary processes. We here study the influence of large-mammal herbivory on the survival and height growth of trees in a mixed species restoration planting of the Australian forest trees, <i>Eucalyptus ovata</i> and <i>E. pauciflora</i>, in Tasmania, Australia. Common-garden field trials mixing the two species were compared in adjacent unbrowsed (fenced) and browsed (unfenced) plantings. The browsed planting was exposed to mammal browsing by native marsupials, as well as feral introduced European fallow deer (<i>Dama dama</i>). Each tree species was represented by open-pollinated families from 22 paired geographic areas, allowing the assessment of the effects of browsing on the species and population differences, as well as on family variation within each species. In the browsed planting, a marked reduction in species and population differences, as well as in family variance, was observed for both height growth and survival. The pattern of height growth and survival of the populations of both species also differed between browsing regimes, with significant changes of climate relationships involving both focal tree attributes detected. Our results argue for a major disruption of the eco-evolutionary dynamics of restored forests in the presence of browsing by large mammalian herbivores, at the observed period of the tree life cycle. Importantly for forest restoration and conservation in the face of global change, our results challenge the choice of tree populations for translocation based solely on predicted or observed relationships of their home-site climate with current and predicted future climates of the restoration sites, while emphasising the need for genetic diversity to provide future resilience of restored forests to both biotic and abiotic stresses.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CpG Landscape of Protein Coding DNA in Vertebrates","authors":"Justin J. S. Wilcox,&nbsp;James Ord,&nbsp;Dennis Kappei,&nbsp;Toni I. Gossmann","doi":"10.1111/eva.70101","DOIUrl":"https://doi.org/10.1111/eva.70101","url":null,"abstract":"<p>DNA methylation has fundamental implications for vertebrate genome evolution by influencing the mutational landscape, particularly at CpG dinucleotides. Methylation-induced mutations drive a genome-wide depletion of CpG sites, creating a dinucleotide composition bias across the genome. Examination of the standard genetic code reveals CpG to be the only facultative dinucleotide; it is however unclear what specific implications CpG bias has on protein coding DNA. Here, we use theoretical considerations of the genetic code combined with empirical genome-wide analyses in six vertebrate species—human, mouse, chicken, great tit, frog, and stickleback—to investigate how CpG content is shaped and maintained in protein-coding genes. We show that protein-coding sequences consistently exhibit significantly higher CpG content than noncoding regions and demonstrate that CpG sites are enriched in genes involved in regulatory functions and stress responses, suggesting selective maintenance of CpG content in specific loci. These findings have important implications for evolutionary applications in both natural and managed populations: CpG content could serve as a genetic marker for assessing adaptive potential, while the identification of CpG-free codons provides a framework for genome optimization in breeding and synthetic biology. Our results underscore the intricate interplay between mutational biases, selection, and epigenetic regulation, offering new insights into how vertebrate genomes evolve under varying ecological and selective pressures.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and Physiological Basis of Structural and Foliar Trait Variation in Tropical Species Pterocarpus officinalis: Implications for Restoration in Future Drier Climates","authors":"Sean M. Collins,&nbsp;Kevin C. Grady,&nbsp;Gustavo P. Lorenzana,&nbsp;Kailey Baesen,&nbsp;Laura Figueroa-Corona,&nbsp;E. Petter Axelsson,&nbsp;Aalap Dixit,&nbsp;Amanda R. De La Torre","doi":"10.1111/eva.70102","DOIUrl":"https://doi.org/10.1111/eva.70102","url":null,"abstract":"<p>Tropical wetlands are some of the most threatened ecosystems in the world. <i>Pterocarpus officinalis</i> exists in swampy wetlands in riparian and fresh-water coastal areas across the neotropics, supporting biodiversity and storm surge and flooding protection as well as water filtration. In Puerto Rico, <i>P. officinalis</i>-dominated forests have been severely declining in recent decades, mainly due to land development. Reversing this trend in the face of climate change and projected sea level rise via ecological restoration may benefit from understanding phenotypic traits suitable for future climates. Currently, there are no seed sourcing guidelines for restoration, due to the understudied nature of the species. The goals of our study were to examine population structure and the genomic basis of variation in structural and physiological foliar traits. Seeds were collected from twelve seed sources spanning the natural distribution of <i>P. officinalis</i> in Puerto Rico. Water use efficiency related foliar traits were measured in well-watered conditions from seedlings grown in a nursery experiment. A total of 109 seedlings were whole-genome resequenced from 12 seed sources. Our results indicate strong foliar trait variation despite very little genetic differentiation among seed sources within the island, suggesting a relatively small number of genes might be involved in water-use efficiency traits. Eleven out of thirteen foliar traits varied significantly across seed sources. Trait variation was associated with either longitude, elevation, mean annual precipitation, or isothermality. Seedlings across seed sources were observed to have different strategies for managing water use. Candidate loci identified using Genome-Wide Association Studies were associated with signal transduction, transcription regulation, DNA and RNA methylation, transport, and primary and secondary metabolism. Restoration of this species is key in maintaining ecosystem services. Our study identified seed sources that may be successful in drier restoration conditions and match future arid climates.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome of Kumamoto Oyster Crassostrea sikamea Provides Insights Into Bivalve Evolution and Environmental Adaptation","authors":"Sheng Liu,&nbsp;Youli Liu,&nbsp;Ximing Guo,&nbsp;Naoki Itoh,&nbsp;Guangqiu Chang,&nbsp;Zhihua Lin,&nbsp;Qinggang Xue","doi":"10.1111/eva.70100","DOIUrl":"https://doi.org/10.1111/eva.70100","url":null,"abstract":"<p>The Kumamoto oyster, <i>Crassostrea sikamea</i>, is a marine bivalve naturally distributed along the coasts of southern China and southern Japan, with a hatchery population that has been under domestication in the United States since its introduction from Japan in the 1940s. To understand its evolutionary history and environmental adaptation, we produced a chromosome-level genome assembly of <i>C. sikamea</i> and conducted whole-genome resequencing of 141 individuals from the US hatchery population and six wild populations from China and Japan. The assembled genome of <i>C. sikamea</i> has a size of 616 Mb covering all 10 chromosomes with a contig N50 of 4.21 Mb and a scaffold N50 of 62.25 Mb. Phylogenetic analysis indicated that <i>C. sikamea</i> diverged from the <i>Crassostrea angulata</i> and <i>Crassostrea gigas</i> clade about 9.9 million years ago. Synteny analysis revealed significant chromosomal rearrangements during bivalve evolution leading to oysters, but remarkable conservation of all 10 oyster chromosomes over ~180 million years, a surprising disparity in chromosomal evolution. Phylogenetic analysis produced three distinct clusters for the US, Japanese, and Chinese populations, with the US population closer to the Japanese population, confirming its origin. No differentiation was detected among the five Chinese populations, indicating strong gene flow. Between the US and Japan populations, 402 genes exhibited selection signals, including three myosin heavy chain genes that were also differentiated in domesticated lines of the eastern oyster, suggesting changes in these genes may be important for domestic production. Among the 768 genes showing selection signals between natural populations of Japan and China, genes related to stress response are most enriched, suggesting responding to environmental stress is critical for local adaptation. These findings provide insights into bivalve evolution and environmental adaptation, as well as useful resources for comparative genomics and genetic improvement of cultured Kumamoto oyster stocks.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroimmune Pain and Its Manipulation by Pathogens","authors":"Kevin W. Lozo,&nbsp;Athena Aktipis,&nbsp;Joe Alcock","doi":"10.1111/eva.70098","DOIUrl":"https://doi.org/10.1111/eva.70098","url":null,"abstract":"<p>Recent studies highlight extensive crosstalk that exists between sensory neurons responsible for pain and the immune system. Cutaneous pain neurons detect harmful microbes, recruit immune cells, and produce anticipatory immunity in nearby tissues. These complementary systems generally protect hosts from infections. At the same time, neuroimmune pain is vulnerable to manipulation. Some pathogens evade immunity activated by nociceptors by producing opioid analogs and by interfering with sensory nerve function. Other organisms manipulate neuroimmune pain by increasing it. Hosts may gain protection from interference by adjusting pain sensitivity. Nociceptive sensitization follows expectations of signal detection theory and the smoke detector principle, allowing pain to be more easily triggered in response to microbial threats and damage. However, pain sensitization at the spinal level and cortical responses to pain are themselves the target of manipulation by parasites and other organisms. Here we review examples of parasites, bacteria, and other medically important organisms that interfere with pain signaling and describe their implications for public health, infectious disease, and the treatment of pain.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene Flow and Abundance of a Tropical Fruit Fly in a Horticultural Landscape Mosaic in Eastern Australia Is Limited by Cleared Grazing Land and Area-Wide Management","authors":"James L. Ryan,&nbsp;Anthony R. Clarke,&nbsp;Alexander M. Piper,&nbsp;Susan Fuller,&nbsp;Peter J. Prentis","doi":"10.1111/eva.70097","DOIUrl":"https://doi.org/10.1111/eva.70097","url":null,"abstract":"<p>Landscape ecology and genetics provide important analytical frameworks for investigating the effect of environmental features on ecological processes. Few empirical studies, however, have simultaneously tested how landscape characteristics influence spatial patterns of gene flow and abundance of pest insects in heterogeneous environments. To address this, we undertook a combined landscape-ecology/landscape-genetic study of the tephritid fruit fly, <i>Bactrocera tryoni</i> , in the Wide Bay-Burnett region of Southeast Queensland, Australia. This region contains areas of rainforest, <i>Eucalyptus</i> forest, cleared pasture, residential areas, and two areas of intensive horticulture production; one implementing area-wide management practices. We collected <i>B. tryoni</i> samples from 26 sites in 2021 during the months of April, August, October, December and the following year during February and April. We used high-density DArTseq SNP genotyping on samples collected during the 2021 April, August and December sampling periods. We then modelled the contemporary landscape characteristics and management factors influencing gene flow and abundance of this pest species. Genome-wide SNP analysis estimated infinite effective population sizes at all sites and detected limited genetic structure across the landscape. However, fly abundance varied significantly among habitats, with cleared pasture negatively associated with population abundance and acting as a barrier to gene flow. Additionally, highways in composite with cleared pasture exhibited a very strong barrier effect. Abundance was highest in residential areas and rainforest, lowest in <i>Eucalyptus</i> forest, and reduced in the horticultural region with area-wide management implemented. We discuss the benefits of collecting simultaneous genetic and ecological datasets for informing and evaluating area-wide management programmes for insect pests and highlight considerations in the spatial analysis of SNP data when effective population sizes are extremely large.</p>","PeriodicalId":168,"journal":{"name":"Evolutionary Applications","volume":"18 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eva.70097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}