Journal of Plant Pathology最新文献

{"title":"Determination of tolerance to Erwinia amylovora of intergeneric hybrid genus: Cydomalus","authors":"Müge Şahin","doi":"10.1007/s42161-024-01692-2","DOIUrl":"https://doi.org/10.1007/s42161-024-01692-2","url":null,"abstract":"<p>A greater genetic diversity is essential for disease resistance in particular, and intergeneric hybridization makes this possible. The genus <i>Cydomalus</i> (syn: <i>Cydolus</i>), which is frequently used as rootstock, was derived from intergeneric hybridization between <i>Cydonia oblonga</i> Mill. and <i>Malus domestica</i> Borkh in Moldova and Bulgaria. It is known that hybrids from this genus exhibit quince and/or apple characteristics and some of them have been identified with reddish fruit skin color in Türkiye in the last decade. <i>Erwinia amylovora</i> Burril. is the most devastating bacterial disease in the Rosaceae family, and there have been no reports of <i>Cydomalus.</i> In this study, fire blight susceptibility index of three <i>Cydomalus</i> genotypes, were collected from İzmir and Manisa provinces, and two important quince rootstocks were determined by artificial inoculations with the most virulent <i>Erwinia amylovora</i> strain of our collection, under greenhouse conditions for two consecutive years. In addition, susceptibility levels of two <i>Cydomalus</i> genotypes were observed under natural disease pressure habitats. In line with the results of artificial inoculations, two of <i>Cydomalus</i> genotypes were determined to be more tolerant to fire blight disease than quince rootstocks and these genotypes could be used in breeding studies. Moreover, <i>Cydomalus</i> genus was identified as the new host for <i>Erwinia amylovora.</i></p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"29 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential oils used against zucchini powdery mildew: evaluation of variability in chemical composition and bioactivity","authors":"Lucia Donnarumma, Paolo Nota, Giovanni Di Lernia, Filomena Milano","doi":"10.1007/s42161-024-01693-1","DOIUrl":"https://doi.org/10.1007/s42161-024-01693-1","url":null,"abstract":"<p>Trend in the reduction on use of synthetic pesticides is continuously growing. Their substitution with natural substances is well studied because of the environmental vantages. Consumers require safe foods. Their risk perception concerns the presence of synthetic pesticides and marketing is oriented toward food with lower and lower residue levels. Essential oils (EOs) could be a reliable alternative. Their components are in a variable range of concentrations determining, in some cases, for the chemotypes. Lots of rosemary, oregano, and clove oil were purchased on the market and their main components were analyzed. Four EOs formulations were prepared to test their effectiveness against zucchini powdery mildew. Moreover, rosemary leaves were collected from the CREA-DC garden (Rome) and rosemary essential oil, distilled in-house, in combination with oregano essential oil were also tested. Metrafenone and penconazole were synthetic fungicides used for reference treatments. Two trials were carried out in a greenhouse.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"16 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into Agrobacteria inducing galls on raspberry","authors":"José Eduardo Godínez-Alemán, Hilda Victoria Silva-Rojas, Andrés Aguilar-Granados, Paulino Pérez-Rodríguez, Alejandrina Robledo-Paz, Angel Rebollar-Alviter","doi":"10.1007/s42161-024-01676-2","DOIUrl":"https://doi.org/10.1007/s42161-024-01676-2","url":null,"abstract":"<p>Raspberry (<i>Rubus idaeus</i> L.) is a crop with increasing commercial value due to its nutritional properties and demand in national and international markets. Throughout the growing process, there have been reports of the presence of galls on roots and stems, which reduce plant size and yields. The current study used phylogenetic reconstruction to identify bacteria associated with these symptoms in raspberry plantations in the Mexican states of Jalisco and Michoacán. A total of 69 representative strains selected according to their colony morphotype were identified based on partial sequencing of the 16S ribosomal gene. Forty-nine of them were tested for pathogenicity, including of which three strains induced gall symptoms in raspberry and tomato plants. A set of 28 strains identified within the <i>Agrobacterium</i> and <i>Rhizobium</i> genera were chosen for amplification of the <i>atpD</i>, <i>glnA</i>, <i>gyrB</i>, and <i>rpoB</i> genes. Strain CPO 2.419 was selected for whole genome sequencing, with a total length of 5,679,921 base pairs assembled into 46 contigs. This strain was identified as <i>A. tumefaciens</i> (GenBank JAVIYJ000000000.1) using phylogenetic reconstruction. According to the OrthoANIu and dDDH percentage values, the CPO 2.419 strain belongs to the <i>A. tumefaciens</i> complex, along with the closely related strains of <i>A. tumefaciens</i> LMG 232 (99.4, 89.1), <i>A. tumefaciens</i> CNPSo 675 (98.5, 84.8), and <i>A. tumefaciens</i> ATCC 4720^T (98.1, 78), respectively. This study contributes to the understanding of the genomic <i>Agrobacterium</i> diversity in raspberry-producing areas in Mexico and highlights the relevance of genomics for accurate bacterial identification, with important implications for agriculture.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"32 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the wheat seed core mycobiome of two Egyptian cultivars (Giza 171 and Sids 14) by using high throughput amplicon sequencing of the ITS2 region","authors":"Sobhy S. H. Abdelsalam, Muziri Mugwanya, Ahmed G. Gad, Ayman B. A. Basyony","doi":"10.1007/s42161-024-01689-x","DOIUrl":"https://doi.org/10.1007/s42161-024-01689-x","url":null,"abstract":"<p>Wheat (<i>Triticum aestivum</i> L.) is an important food crop throughout the world. The seed mycobiome is the primary inoculum affecting the overall health and productivity of wheat plants. Seed-borne fungi can have an impact on the production of wheat and cause health complications upon consumption. Therefore, for durable and sustainable wheat production, it is imperative to characterise the wheat mycobiome. The rationale of this study was to investigate the wheat seed mycobiome of two Egyptian wheat cultivars (Sids 14 and Giza 171) using a culture-independent technique. The fungal community in 6 wheat seed samples was identified by high-throughput amplicon sequencing of the ITS2 region using the Illumina MiSeq platform. A total of 162 genera, 111 families, 55 orders and 25 classes have been identified. The fungal communities detected varied between seed samples, with a higher abundance of <i>Ascomycota</i> followed by <i>Basidiomycota</i>. Potential beneficial and pathogenic genera could be detected. Interestingly, a higher abundance of <i>Vishniacozyma</i>, <i>Epicoccum</i>, <i>Cladosporium</i>, <i>Blumeria</i>, <i>Stymphylium</i>, <i>Fusarium</i>, <i>Filobasidium</i>, and <i>Alternaria</i> was observed in the samples. Both <i>Anthracocystis</i> and <i>Nigrospora</i> were detected exclusively in <i>T. aestivum</i> cv. Giza 171. These results open new avenues for further investigation on the role of the mycobiome in Egyptian wheat cultivars. Ultimately, precise identification of both beneficial and pathogenic fungal genera could be accomplished.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salicylic acid blocks rice leaf blight by inhibiting biofilm formation of Xanthomonas oryzae pv. oryzae in rice","authors":"Sudha A, Fahima Firdouse M, Selva Amala A, Khalid E. Hamed","doi":"10.1007/s42161-024-01668-2","DOIUrl":"https://doi.org/10.1007/s42161-024-01668-2","url":null,"abstract":"<p><i>Xanthomonas oryzae</i> pv. <i>oryzae</i> is a destructive bacterial disease of rice. Due to the development of resistance by the existing bactericides, an alternative eco-friendly management strategy should be identified to contain the disease. The present study focused on the efficacy of salicylic acid (SA) and their metabolically active biomolecules against <i>X. oryzae</i> pv. <i>oryzae</i> during their interaction. The biofilm inhibition clearly expressed the aggregation, separation and presence of dead cells at various concentrations of SA. The highest biofilm production was observed for untreated control with maximum O.D. value. There is a significant difference in their O.D. values on the concentrations of SA. While studying the inhibition percentages, the chloramphenicol @ 0.2% recorded the highest percentage (76.65%) which shows significant results with SA at 250 ppm (75.56) followed by 100 and 50 ppm which exhibited no significant differences in their inhibition percentages (65.78% and 45.74%, respectively). Under CLSM the <i>Xoo</i> cell aggregation and separation has been observed at 50 ppm and 100 ppm and diminished at 250 ppm respectively. Under <i>in silico</i> analysis, the biomolecules from SA, viz., tetradecanoic acid, methyl oleate, and decyl acrylate, were extracted with a higher area percentage against <i>Xoo</i> target proteins with chloramphenicol as a positive control. Tetradecanoic acid has the highest -C DOCKER energy of 39.46 kcal/mol with the effector proteins of <i>Xoo</i>. The docked metabolites, were subjected to <i>in planta</i> assays, among them tetradecanoic acid recorded minimum lesion size of 0.6 cm at 5000 ppm followed by decyl acrylate (1.5 cm).Our results suggest that overexpression of NPR 1, PAL and LOX has been documented at SA at 250 ppm which can be used to improve BLB resistance in rice. The present study concludes that salicylic acid plays a signalling role inhibition of <i>Xoo</i> biofilm, a source of biomolecules, activation of various plant defence responses against <i>Xoo.</i></p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"37 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome assembly of two California isolates of Seiridium cardinale (BM-138-000494 and BM-138-000479)","authors":"Edoardo Scali, Gianni Della Rocca, Roberto Danti, Matteo Garbelotto, Sara Barberini, Giovanni Emiliani","doi":"10.1007/s42161-024-01665-5","DOIUrl":"https://doi.org/10.1007/s42161-024-01665-5","url":null,"abstract":"","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"44 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Hemileia vastatrix resistance in chemically mutagenized Coffea arabica L. leaf discs and the emergence of a novel resistance scale","authors":"José Andrés Rojas-Chacón, Fabián Echeverría-Beirute, Bradley J. Till, Andrés Gatica-Arias","doi":"10.1007/s42161-024-01620-4","DOIUrl":"https://doi.org/10.1007/s42161-024-01620-4","url":null,"abstract":"<p>Coffee leaf rust (CLR), caused by <i>Hemileia vastatrix</i> Berk. and Br, stands as a globally significant disease responsible for diminishing the grain yield and quality of <i>Coffea arabica</i> L. As such, the need arises for a practical and dependable method to quantify CLR disease severity and propose effective management strategies. The creation of a diagrammatic scale, featuring color photographs, proves instrumental in enhancing severity assessment and disease estimation. Mutagenesis serves as a potent tool for improving crop traits, notably resistance to pests and diseases, by inducing genetic variability, thus fostering phenotypic diversity. The primary objective of this study was to craft a novel diagrammatic scale for leaf discs, designed to gauge the severity of coffee leaf rust in <i>C. arabica</i> L. cv. Catuaí plants treated with sodium azide (NaN3) and ethyl methane sulfonate (EMS). The assessment encompassed a thorough examination of incidence, severity, incubation period, and latency in plant leaf discs inoculated with CLR isolates, all carried out under rigorously controlled laboratory conditions. The scale’s construction hinged on the frequency distribution of severity and Weber-Fechner’s visual stimulus law, which facilitated the determination of minimum and maximum limits, as well as intermediary levels. The resulting scale comprises seven distinct levels spanning a range from 0 to 52.15% of the afflicted leaf area. Employing conglomerate analysis (Wilks, <i>p</i> &lt; 0.0001) unveiled four groups of progenies exhibiting significantly different (<i>p</i> &lt; 0.05) resistance responses to CLR. Notably, Plant E154 displayed an incidence rate of merely 0.3% for CLR, while the remaining plants, including A123, E6, E64, A127, CES5, A107, A93, E29, E116, and A101, showcased an incidence rate of less than 10%. In conclusion, this scale represents a suitable, valuable, and dependable tool for visually estimating CLR severity in coffee leaf discs.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"16 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141532367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of durum wheat vs. bread wheat to Fusarium foot and root rot under semi-arid conditions","authors":"Samira Chekali, Sourour Ayed, Eya Khemir, Mohamed Saleh Gharbi, Salem Marzougui, Timothy Paulitz, Samia Gargouri","doi":"10.1007/s42161-024-01659-3","DOIUrl":"https://doi.org/10.1007/s42161-024-01659-3","url":null,"abstract":"<p>Fusarium foot and root rot is one of the most important soilborne diseases of cereals, specifically on durum wheat which is the main cultivated species in the Mediterranean regions. This work aimed to evaluate the response of durum wheat and bread wheat to this disease under different climatic conditions. The disease parameters, grain yield loss as well as thousand kernel weight (TKW), heading date and height of plant were evaluated on 29 durum and bread wheat genotypes during two years. Overall, durum wheat grain yield was strongly and negatively correlated with disease parameters, heading date, and positively with plant height and TKW compared to bread wheat. In the drier year, the 24 durum wheat genotypes were more susceptible to the disease than the five bread wheat varieties while some of them showed less disease during the wetter year. Bread wheat maintained the same behavior in both years vs. durum wheat. Grain yield loss was higher on durum wheat compared to bread wheat. It was the same in both years (56 and 51%) for durum wheat and was significantly (<i>p</i> &lt; 0.01) lower in the drier (24%) compared to the wetter year (44%) for bread wheat. This work confirmed the susceptibility of Tunisian durum wheat to the disease compared to bread wheat and calls attention to the potential vulnerability of this important Mediterranean food crop under a hotter, drier climate. More research is needed to investigate adaptation to drought and plant defense mechanisms and to breed for resistance to this disease.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"167 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141253690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fire4CAST – a new integrative epidemiological forecasting model for the accurate prediction of infection risk and effective control of fire blight in Pyrus orchards","authors":"Daniel McGuire, Francisco Pinto, Telma Costa, Joana Cruz, Rui Sousa, Miguel Leão de Sousa, Carmo Martins, Margarida Gama-Carvalho, Ana Tenreiro, Rogério Tenreiro, Leonor Cruz","doi":"10.1007/s42161-024-01622-2","DOIUrl":"https://doi.org/10.1007/s42161-024-01622-2","url":null,"abstract":"<p>Fire blight disease, caused by <i>Erwinia amylovora</i> is present worldwide and affects over 40 countries in Europe where it is listed as a quarantine or regulated pest often due to ineffective control strategies maladapted to the respective production systems. In Portugal, the disease was confirmed in 2010 and the occurrence of disease outbreaks in new production areas has risen over the years. The disease affects the national production of apple and pear fruits, with greater impact on the national pear variety ‘Rocha’, widely exported to European countries and Brazil. The mild temperatures and high relative humidity promote the progression of the disease during winter, revealing the potential activity of the bacterium in the latency period (LP) of the host. Infection alert risk using the established predictive models Maryblight TM, Cougarblight and BIS98 was put in place in 2013 by Centro Operativo e Tecnológico Hortofrutícola Nacional (COTHN). These attempts to control the spread of this disease, showed low accuracy for the Portuguese epidemiological reality. Within the framework of project Fire4CAST we developed a new epidemiological model to predict fire blight disease using a systems biology approach integrating microbiological, cytological and genomic pathogen data with phenological host development and climatic variables. The presence of <i>E. amylovora</i> was monitored in orchards with fire blight history using standard laboratory tests. Simultaneously, the implementation of immune-flow cytometry (IFCM) highlighted the viability of <i>E. amylovora</i> populations prevailing during winter and early spring, long before bloom risk period. The integration of the whole data set allowed the development of the Fire4CAST predictive model, able to monitor the expected infection date (EID) and to define accurate outbreak alarms. Fire4CAST model enabled the detection of outbreak risk during winter based on rules that consider climatic data variables, which were validated by effective presence of live and active <i>E. amylovora</i> populations and real-time quantitative PCR (qPCR) data, accomplishing a precision rate of 83%. Field application of Fire4CAST can hopefully guide the implementation of successful control strategies, leading to more sustainable pome chain production areas.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"46 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141253689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyricularia pennisetigena as leaf blast disease-causing pathogen in king grass (Pennisetum sinese) and its assessment of the pathogenic risk","authors":"Yinglong Liu, Tengfei Gui, Ayesha Ahmed, Shahzad Munir, Pengfei He, Pengbo He, Yixin Wu, Ping Tang, Qiong Luo, Yueqiu He","doi":"10.1007/s42161-024-01654-8","DOIUrl":"https://doi.org/10.1007/s42161-024-01654-8","url":null,"abstract":"<p>King grass (<i>Pennisetum sinese</i>), also known as sugar cane grass, is being affected by emerging leaf blast pathogen which is responsible for causing foliar disease in Baoshan City, Yunnan Province, China. The pathogen was isolated from the typical infected plants showing symptoms like eye-shaped and whitish color in the center with brown-black necrotic borders surrounded by a yellow halo sample on potato dextrose agar (PDA) media. The leaves of various plants including maize (<i>Zea mays</i>), hybrid rice (<i>Oryza sativa</i>), and wheat (<i>Triticum aestivum</i>), were inoculated with the pathogen in an in vitro assay to evaluate the infection potential of the pathogen and transmission risk. Our study verified that Psp1 is pathogenic to the king grass leaves and it is considered as the main causal agent of leaf blast in the study area. Further, morphological characterization and molecular analysis based on ITS region and LSU gene confirmed the pathogen as <i>Pyricularia pennisetigena</i>. During pathogenicity assay, both Psp1 and LP11 isolates of a rice blast pathogen induced symptoms of varying severity in a total of different nine plants. Among these, the pathogen Psp1 exhibited significant symptoms, including conidial emergence on king grass leaves (<i>Pennisetum alopecuroides</i> and <i>Pe. flaccidum</i>) indicating high pathogenicity to these plants. This suggests that Psp1 pose a considerable a high pathogenic risk to <i>Pennisetum</i> spp. This work lays a groundwork for future implementation of integrated management strategies for leaf blast in local king grass, offering a theoretical basis and research foundation for such endeavor.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141168745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}