Journal of Plant Biochemistry and Biotechnology最新文献_第6页

Functional identification and characterization of two flavonoid glycosyltransferases ZmUGT84A3 and ZmUGT84A4 from maize 玉米中两种黄酮类糖基转移酶 ZmUGT84A3 和 ZmUGT84A4 的功能鉴定和特征描述

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2024-01-28 DOI: 10.1007/s13562-024-00874-4

Zhao Ke, Dengyu Zheng, Meng She, Shipeng Zhang, Zhongyi Wu, Min Lu, Zhongbao Zhang

{"title":"Functional identification and characterization of two flavonoid glycosyltransferases ZmUGT84A3 and ZmUGT84A4 from maize","authors":"Zhao Ke, Dengyu Zheng, Meng She, Shipeng Zhang, Zhongyi Wu, Min Lu, Zhongbao Zhang","doi":"10.1007/s13562-024-00874-4","DOIUrl":"https://doi.org/10.1007/s13562-024-00874-4","url":null,"abstract":"Luteolin is an important secondary metabolite of maize, which can be glycosylated through uridine diphosphate glycosyltransferase (UGT). Extensive research on UGT has been centered in Arabidopsis thaliana, but little in maize. We cloned two maize glycosyltransferase genes, ZmUGT84A3 and ZmUGT84A4. In vitro experiments revealed that ZmUGT84A3 and ZmUGT84A4 glycosylated luteolin to both luteolin-7-O-glucoside and luteolin-4′,7-di-O-glucoside. Notably, both ZmUGT84A3 and ZmUGT84A4 catalyzed glycosylation of many flavonoids including apigenin, naringenin, eriodyctiol, kaempferol, and quercetin. Increased temperature enhanced activities of both ZmUGT84A3 and ZmUGT84A4, and elevated a production of luteolin-4′,7-di-O-glucoside. ZmUGT84A3 activity was optimal under acidic conditions but ZmUGT84A4 preferred alkaline environments. ZmUGT84A4 exhibited higher catalytic activities (1.3 to 2.6-fold) at various substrate concentrations than did ZmUGT84A3; the latter enzyme evidenced a unique activity pattern. Enzyme-catalyzed reaction processes of ZmUGT84A3 and ZmUGT84A4 in vitro for luteolin were depicted. This study unveiled two novel multifunctional glycosyltransferases identified in maize, which exhibited extensive substrate specificity, possessed multiple catalytic sites, and offered valuable insights for studying their enzymatic characteristics.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"10 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139578783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cost-effective multiplex PCR assay for simultaneous detection of bacterial leaf blight, blast and brown planthopper resistance genes in rice 同时检测水稻细菌性叶枯病、稻瘟病和褐斑病抗性基因的经济高效的多重 PCR 分析法

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2024-01-25 DOI: 10.1007/s13562-023-00870-0

{"title":"Cost-effective multiplex PCR assay for simultaneous detection of bacterial leaf blight, blast and brown planthopper resistance genes in rice","authors":"","doi":"10.1007/s13562-023-00870-0","DOIUrl":"https://doi.org/10.1007/s13562-023-00870-0","url":null,"abstract":"<h3>Abstract</h3> Rice production is drastically affected by biotic stresses, namely, bacterial leaf blight (BB), blast (BL) and brown planthopper (BPH). As marker-assisted selection for uniplex gene screening in breeding population is often tedious, time-consuming and expensive, multiplex PCR is practically a reasonable choice for the testing of genes in the pyramided lines as it offers significant time- and cost-saving advantages. Therefore, a cost-effective multiplex PCR-based screening for simultaneous detection of three BB (Xa21, xa13, and Xa4), two BL (Pi1and Pi54) and two BPH resistance genes (Bph20 and Bph21) has been developed. This multiplex system can detect different combinations of BB, BL and BPH resistance. In addition to this most cost-effective method, a five-gene (Xa21, xa13, Pi54, Bph20 and Bph21) multiplex assay in a single PCR tube for three biotic stresses, BB, BL and BPH, has been developed. These standardized multiplex assays have been incorporated into our breeding programs for accelerated results. These assays have displayed no deviation and are in perfect correlation with the individual marker screenings. As one of the most efficient and cost-saving technologies, this technology will have a great impact in the seed industry.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"17 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139578774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping QTLs for grain iron, zinc, and yield traits in advanced backcross inbred lines of Samba mahsuri (BPT5204)/Oryza rufipogon 绘制高级回交近交系 Samba mahsuri (BPT5204)/Oryza rufipogon 谷物铁、锌和产量性状的 QTLs 图谱

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2024-01-02 DOI: 10.1007/s13562-023-00869-7

Gowthami Chandu, Divya Balakrishnan, Suchandranath Babu Munnam, Satendra K. Mangrauthia, Durbha Sanjeeva Rao, C. N. Neeraja, R. M. Sundaram, Sarla Neelamraju

{"title":"Mapping QTLs for grain iron, zinc, and yield traits in advanced backcross inbred lines of Samba mahsuri (BPT5204)/Oryza rufipogon","authors":"Gowthami Chandu, Divya Balakrishnan, Suchandranath Babu Munnam, Satendra K. Mangrauthia, Durbha Sanjeeva Rao, C. N. Neeraja, R. M. Sundaram, Sarla Neelamraju","doi":"10.1007/s13562-023-00869-7","DOIUrl":"https://doi.org/10.1007/s13562-023-00869-7","url":null,"abstract":"Iron and zinc deficiency is a major problem among large populations in rice-consuming countries. Development micronutrient dense rice varieties with high yield is a key target area in breeding programmes and QTL mapping studies using backcross inbred lines to transfer beneficial genes from wild relatives is one of the potential strategy. In this study, 136 BC4F10 backcross inbred lines (BILs) from BPT5204 x Oryza rufipogon WR119 were field evaluated for 3 years for nine yield related traits. Grain Fe and Zn were estimated using ED-XRF. In all, 11 major QTLs with phenotypic variance from 10 to 16.8% were identified for Fe, Zn, and 5 yield related traits. O. rufipogon alleles were trait-enhancing in 18% of all QTLs and an allele at qFe2.1 increased iron concentration. Major effect QTLs qFe1.1 for grain Fe and qZn5.1, qZn8.1, and qZn10.1 for grain Zn explained 11 to 16% PVE, qZn8.1 and qZn10.1 were co-located with QTLs for grain yield related traits. Seven chromosomal regions showed QTLs for more than two traits. QTLs were associated with several high priority candidate genes for grain Fe, Zn and yield. One elite BIL [IET 24775 RP4920-Bio51B] was tested in AICRIP bio fortification trials for 4 years [2014–2017], and three BILs [IET 28715 RP4920-Bio61-1B], [IET28706 RP4920-Bio83B] and [IET28695 RP4920-Bio88B] are evaluated for 2 years of trials. The significant BILs and QTLs are useful in rice bio fortification and for gene discovery.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"104 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139077155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision genetic technologies for cereal functional genomics 谷物功能基因组学的精准遗传技术

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2023-12-18 DOI: 10.1007/s13562-023-00862-0

{"title":"Precision genetic technologies for cereal functional genomics","authors":"","doi":"10.1007/s13562-023-00862-0","DOIUrl":"https://doi.org/10.1007/s13562-023-00862-0","url":null,"abstract":"<h3>Abstract</h3> Identifying and characterizing genes that control important agronomic traits and finding ways to alter them are vital for crop improvement. Map-based cloning, heterologous gene expression, RNAi mediated gene silencing, T-DNA insertional mutation and TILLING technologies have enabled cloning, characterization, and deployment of a few important genes. Revolution in sequencing technologies and bioinformatics has facilitated us to quickly predict and annotate plethora of genes and regulatory elements with improved precision and spatio-temporal expression of genes in almost all cereal crops. However, their functional validation forms a bottleneck in exploiting useful genetic elements for crop improvement. The CRISPR/Cas9 mediated gene editing has become the tool of choice for precisely introducing targeted modifications in the genome to knock out/in a gene, introducing specific mutation in sequence or modulating its expression in diverse crop species. This can help to rapidly characterize plenty of genes in terms of understanding the function of individual gene/ gene family, involvement in a particular biochemical pathway or interaction of the crop plant with external stimuli at a reasonable cost. In this review, we discuss the power of gene editing for rapid functional characterization of genetic elements as a fundamental requirement to harness the power of precision genetic technologies for increasing crop yield, progress made so far, opportunities and challenges.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138746050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of BvNHX1, a novel tonoplast Na+/H+ antiporter gene from sugar beet (Beta vulgaris), confers enhanced salt tolerance in transgenic tobacco 转基因烟草过表达甜菜（Beta vulgaris）中一种新型调质体 Na+/H+ 反转运体基因 BvNHX1 可增强耐盐性

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2023-12-12 DOI: 10.1007/s13562-023-00868-8

Xin-Miao Zhang, Guo-Qiang Wu, Ming Wei, Hong-Xia Kang

{"title":"Overexpression of BvNHX1, a novel tonoplast Na+/H+ antiporter gene from sugar beet (Beta vulgaris), confers enhanced salt tolerance in transgenic tobacco","authors":"Xin-Miao Zhang, Guo-Qiang Wu, Ming Wei, Hong-Xia Kang","doi":"10.1007/s13562-023-00868-8","DOIUrl":"https://doi.org/10.1007/s13562-023-00868-8","url":null,"abstract":"Salinity is one of the major environmental factors that limit the plant growth and crop productivity worldwide. Tonoplast Na+/H+ transporters (NHXs) play crucial roles in regulating the intracellular Na+/K+ and pH homoeostasis, which is essential for salt tolerance and development of plants. In the present study, a novel gene BvNHX1 encoding tonoplast Na+/H+ antiporter was isolated in natrophilic crop sugar beet (Beta vulgaris) and functionally characterized in tobacco (Nicotiana tabacum) plants to assess the behavior of the transgenic organisms in the response to salt stress. The results showed that overexpression of BvNHX1 significantly enhanced salt tolerance in transgenic tobacco plants compared with wild-type (WT) plants. The seed germination, root length, plant height, and fresh and dry weights in transgenic plants were significantly higher than those in WT plants under salt stresses. The contents of leaf relative water, chlorophyll, proline, soluble sugars, and soluble proteins were significantly higher as compared with WT plants, while malondialdehyde (MDA) contents were significantly lower than those of WT plants under salt stresses. Na+ and K+ contents both in shoots and roots of transgenic plants were significantly higher than those of WT plants, and transgenic plants maintained a balanced K+/Na+ ratio under saline conditions. Taken together, these results suggested that overexpression of BvNHX1 reduced damage to cell membrane by reducing osmotic potential of cells, and maintaining relative water and chlorophyll contents of leaves, and finally improved salt tolerance in transgenic tobacco plants.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"5 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138631340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MYB44 plays key roles in regulating plant responses to abiotic and biotic stress, metabolism, and development MYB44在调节植物对非生物和生物胁迫、代谢和发育的反应中起关键作用

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2023-12-02 DOI: 10.1007/s13562-023-00864-y

Fenghua Wang, Feng Yang, Dengfeng Zhu, Boyelayefa Saniboere, Bo Zhou, Dan Peng

{"title":"MYB44 plays key roles in regulating plant responses to abiotic and biotic stress, metabolism, and development","authors":"Fenghua Wang, Feng Yang, Dengfeng Zhu, Boyelayefa Saniboere, Bo Zhou, Dan Peng","doi":"10.1007/s13562-023-00864-y","DOIUrl":"https://doi.org/10.1007/s13562-023-00864-y","url":null,"abstract":"MYB44 played key roles in plant responses to abiotic and biotic stress and important roles in plant metabolism and development. At present, the function of MYB44 has not been systemically summarized in plants. In this review, we systemically summarized the structure and function of MYB44 in plants, such as how MYB44 interplays in phytohormone signaling pathways; how MYB44 regulates abiotic and biotic stress, which includes drought tolerance, salt tolerance, cold tolerance, responding to phosphate and nitrogen starvation, and disease resistance; and how MYB44 regulates plant metabolism, which contains MYB44 regulates fruit malate accumulation, starch biosynthesis, sucrose accumulation, flavonoid accumulation, anthocyanin biosynthesis, chlorophyll degradation, and leaf senescence; it also regulates plant development, which includes root growth and development and somatic embryogenesis in plants. Moreover, we constructed the regulatory networks of the MYB44 protein's responses to biotic and abiotic stress and their regulation of plant metabolism and development. Furthermore, we give some suggestions on how to use MYB44 as a positive and negative regulator to create new breeds in the future.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"59 2","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical, physiological and molecular responses of rice to terminal drought stress: transcriptome profiling of leaf and root reveals the key stress-responsive genes 水稻对末端干旱胁迫的生化、生理和分子响应:叶片和根系的转录组分析揭示了关键的胁迫响应基因

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2023-11-27 DOI: 10.1007/s13562-023-00865-x

Aruna Tyagi, Suresh Kumar, Trilochan Mohapatra

{"title":"Biochemical, physiological and molecular responses of rice to terminal drought stress: transcriptome profiling of leaf and root reveals the key stress-responsive genes","authors":"Aruna Tyagi, Suresh Kumar, Trilochan Mohapatra","doi":"10.1007/s13562-023-00865-x","DOIUrl":"https://doi.org/10.1007/s13562-023-00865-x","url":null,"abstract":"Drought stress has been known to adversely affect growth, development, and productivity of plants to varying extent. Being a multifaceted trait, drought tolerance involves interaction of an array of genes, pathways, and mechanisms. A unique regulatory scheme is adopted by different plants, which provides tolerance to drought stress in association with biochemical and physiological mechanisms. Transcriptome analysis of a drought tolerant [Nagina 22 (N-22)] and drought sensitive (IR-64) cultivars provides insights into the genes/pathways/mechanisms involved in terminal drought stress tolerance. In the present study, comparative physio-biochemical analyses of the rice cultivars under terminal drought stress substantiated their performance. Whole transcriptome analysis of leaf and root from the rice cultivars exposed to terminal drought stress revealed 6077 and 10,050 differentially expressed genes (DEGs) in leaf of N-22 and IR-64, respectively, under drought stress. A maximum of 2682 genes were up-regulated exclusively in N-22 while 7198 genes were down-regulated exclusively in leaf of IR-64. Interestingly, the highest number (2594) of genes was down-regulated exclusively in roots of IR-64, while only 1497 gene were up-regulated exclusively in root of N-22. Differential expression of OsNAC10, OsbZIP23, OsABA8ox1, OsCPK4, OsLEA3, and OsNCED4 along with the GO terms enriched with up-regulated genes for transcription factors (TFs), redox homeostasis, and ABA signaling in N-22 under terminal drought stress play crucial roles in stress tolerance. The stress-responsive genes for transcription factors, redox homeostasis, and ABA signaling up-regulated in N-22 were mainly responsible for terminal drought tolerance. These stress-associated genes can be utilized for genetic improvement of rice for drought tolerance.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"63 2","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The evolving landscape of global regulations on genome-edited crops 全球基因编辑作物监管的演变格局

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2023-11-23 DOI: 10.1007/s13562-023-00863-z

Zarna Vora, Janki Pandya, Chandramohan Sangh, Papa Rao Vaikuntapu

{"title":"The evolving landscape of global regulations on genome-edited crops","authors":"Zarna Vora, Janki Pandya, Chandramohan Sangh, Papa Rao Vaikuntapu","doi":"10.1007/s13562-023-00863-z","DOIUrl":"https://doi.org/10.1007/s13562-023-00863-z","url":null,"abstract":"The creators of CRISPR/Cas have been awarded the 2020 Nobel Prize in Chemistry for their ground-breaking technology and its exceptional potential to address fundamental issues in the field of biological sciences. This revolutionary tool has accelerated the development of novel crop varieties with enhanced features in agriculture, all without the need for transgenes. However, in order for this technology to reach its full potential, the establishment of a precise and comprehensive global regulatory framework for these crops is crucial. Despite the absence of foreign genetic material in crops developed through CRISPR/Cas mediated genome editing, there is an ongoing and intense debate surrounding the regulation of these crops prior to their release into the market. While certain CRISPR-edited crops have already been introduced in Japan, their legal status remains a point of contention in several nations, including the EU and New Zealand. This review paper serves as a comprehensive guide to the worldwide regulatory framework for CRISPR-edited crops, as well as provide insights into the future prospects of this transformative technology. By examining the current landscape of regulations and exploring potential avenues for harmonization, we can better understand the challenges and opportunities that lie ahead for CRISPR-edited crops.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"71 2","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genome editing in plants: a tool for precision breeding and functional genomics 植物基因组编辑:精确育种和功能基因组学的工具

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2023-11-23 DOI: 10.1007/s13562-023-00867-9

Viswanathan Chinnusamy, Van Schepler-Luu, Satendra K. Mangrauthia, S. V. Ramesh

{"title":"Genome editing in plants: a tool for precision breeding and functional genomics","authors":"Viswanathan Chinnusamy, Van Schepler-Luu, Satendra K. Mangrauthia, S. V. Ramesh","doi":"10.1007/s13562-023-00867-9","DOIUrl":"https://doi.org/10.1007/s13562-023-00867-9","url":null,"abstract":"Genome or gene editing (GE) involves a repertoire of innovative molecular techniques that make use of sequence-specific nucleases (SSNs), for the precise modification of an organism's genome sequences. The CRISPR/Cas-based GE system, associated with Clustered Regularly Interspaced Short Palindromic Repeats, has emerged as a potent addition to the expanding genomics toolkit. It enables precise mutagenesis, gene knockouts, multiplex gene editing, and the manipulation of gene expression in plants. Undoubtedly, the application of CRISPR/Cas-based GE in plants has brought about a revolution in basic research, aiding in our understanding of gene functions and significantly advancing applied crop research. This, in turn, underscores its immense potential for crop improvement. Against this backdrop, the current Special Issue on \"Genome Editing in Plants: A Tool for Precision Breeding and Functional Genomics\" represents a timely effort to assemble a group of leading experts in the field of plant genome editing. This compilation includes a commentary article, two original research papers, and eleven review articles and is expected to bring about substantial progress in the field of plant science, particularly in the domain of genome editing.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"66 2","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9 mediated editing of phytoene desaturase gene in squash CRISPR/Cas9介导的南瓜植物烯去饱和酶基因编辑

IF 1.9 4区生物学

Journal of Plant Biochemistry and Biotechnology Pub Date : 2023-11-20 DOI: 10.1007/s13562-023-00866-w

Shallu Thakur, Geoffrey Meru

{"title":"CRISPR/Cas9 mediated editing of phytoene desaturase gene in squash","authors":"Shallu Thakur, Geoffrey Meru","doi":"10.1007/s13562-023-00866-w","DOIUrl":"https://doi.org/10.1007/s13562-023-00866-w","url":null,"abstract":"Gene editing using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated 9 (CRISPR/Cas9) system has become an important biotechnological tool for studying gene function and improving crops. In the present study, the potential of the system was assessed for squash (Cucurbita pepo subspecies pepo) by targeting phytoene desaturase (PDS) gene using the particle bombardment method. The recombinant pHSE401 vector, carrying two sgRNAs (gRNA1 and gRNA2) specific to the PDS homolog (Cp4.1LG08g06310, CpPDS) under the control of Arabidopsis U6 promoter and the Cas9 protein was developed and bombarded into cotyledonary node explants of squash cv. Black Beauty. The transformation efficiency of 4.5% was observed and all the transformants exhibited albino/bleached phenotype. The CpPDS knockout system generated easily detectable bleached/albino explants within 6–8 weeks. The albino phenotype was confirmed through Sanger sequencing which detected several deletion mutations (single, two and three bp deletion) within the CpPDS-gRNA1 target. However, no mutations were found within the CpPDS-gRNA2 target. This study demonstrated CRISPR/Cas9 as a viable tool for gene editing in squash and provides a platform for the modification of economically important traits in the crop.","PeriodicalId":16835,"journal":{"name":"Journal of Plant Biochemistry and Biotechnology","volume":"58 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1