Viswanathan Chinnusamy, Van Schepler-Luu, Satendra K. Mangrauthia, S. V. Ramesh
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引用次数: 0
摘要
基因组或基因编辑(GE)涉及一系列创新的分子技术,这些技术利用序列特异性核酸酶(ssn)来精确修饰生物体的基因组序列。基于CRISPR/ cas的GE系统,与集群规则间隔短回文重复序列(Clustered Regularly Interspaced Short Palindromic Repeats)相关,已成为不断扩展的基因组学工具包的有力补充。它可以实现精确的诱变、基因敲除、多重基因编辑和操纵植物中的基因表达。毫无疑问,基于CRISPR/ cas1的转基因技术在植物中的应用为基础研究带来了一场革命,帮助我们了解基因功能,极大地推进了应用作物的研究。这反过来又强调了它在作物改良方面的巨大潜力。在此背景下,本期《植物基因组编辑:精确育种和功能基因组学的工具》特刊及时召集了一批植物基因组编辑领域的顶尖专家。该汇编包括1篇评论文章、2篇原创研究论文和11篇综述文章,预计将在植物科学领域,特别是基因组编辑领域带来实质性进展。
Genome editing in plants: a tool for precision breeding and functional genomics
Genome or gene editing (GE) involves a repertoire of innovative molecular techniques that make use of sequence-specific nucleases (SSNs), for the precise modification of an organism's genome sequences. The CRISPR/Cas-based GE system, associated with Clustered Regularly Interspaced Short Palindromic Repeats, has emerged as a potent addition to the expanding genomics toolkit. It enables precise mutagenesis, gene knockouts, multiplex gene editing, and the manipulation of gene expression in plants. Undoubtedly, the application of CRISPR/Cas-based GE in plants has brought about a revolution in basic research, aiding in our understanding of gene functions and significantly advancing applied crop research. This, in turn, underscores its immense potential for crop improvement. Against this backdrop, the current Special Issue on "Genome Editing in Plants: A Tool for Precision Breeding and Functional Genomics" represents a timely effort to assemble a group of leading experts in the field of plant genome editing. This compilation includes a commentary article, two original research papers, and eleven review articles and is expected to bring about substantial progress in the field of plant science, particularly in the domain of genome editing.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.