Journal of Plant Research最新文献

{"title":"Quantitative western blotting adapted for expanded sample capacity with non-linear curve fitting.","authors":"Yuting Cheng, Junko Kishimoto, Zihao Ye, Ayu Narita, Xueyun Hu, Hisashi Ito, Ryouichi Tanaka","doi":"10.1007/s10265-026-01700-9","DOIUrl":"10.1007/s10265-026-01700-9","url":null,"abstract":"<p><p>Quantification of cellular protein levels is a central aspect of molecular biology. Although large-scale proteomic quantification technologies have been developed, Western blotting remains a widely used method for researchers investigating a limited number of target proteins. As the demand for rigorous statistical evaluation of Western blotting data increases, particularly in studies involving larger sample sizes, the limitations imposed by the finite number of samples that can be loaded onto a single SDS-PAGE gel have become more apparent. Consequently, the quantitative capacity of Western blotting experiments is often restricted. To address this, we developed an enhanced method for quantitative Western blotting using a multi-gel blotting approach, which was originally described by Aksamitiene et al. for high-throughput Western blotting, wherein multiple SDS-PAGE gels are simultaneously transferred onto a single PVDF membrane. To optimize the accuracy of quantification, transfer conditions were systematically examined, and a standard protocol with which the majority of protein is uniformly transferred to a membrane is provided. Furthermore, considering the inherent non-linear relationship of protein concentration and Western blotting signal intensity, we adopted a log-logistic regression model to calibrate the relationship for the estimation of protein concentrations in unknown samples. Taken together, an enhanced quantitative Western blotting method is provided, offering improved accuracy and reliability in protein quantification.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"317-329"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147307179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockout of a single aquaporin, OsPIP2;4, decreases root water permeability in rice.","authors":"Aya Onishi, Tomoaki Horie, Ryo Ishitsuka, Shizuka Sasano, Rie Horie, Yunosuke Mito, Shigeko Utsugi, Junko Ishikawa, Majid Mahdieh, Maki Katsuhara","doi":"10.1007/s10265-025-01691-z","DOIUrl":"10.1007/s10265-025-01691-z","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"255-265"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12992421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Floral vascularization aiding in unraveling morphologically complex flowers of Apocynaceae.","authors":"Daniela M Alves, Letícia S Souto, Juliana L S Mayer, Ingrid Koch","doi":"10.1007/s10265-025-01686-w","DOIUrl":"10.1007/s10265-025-01686-w","url":null,"abstract":"<p><p>Apocynaceae exhibit remarkable floral morphological diversity, primarily through increased synorganization between the androecium and the gynoecium. However, internal structures such as floral vascularization remain underexplored. This study investigated vascular patterns in different morphological groups within the family, focusing on progressive adnation between stamens and carpels to better understand morphological variation and floral evolution. Floral buds and open flowers from five Apocynaceae species were analyzed using histological techniques, 3D reconstructions via X-ray microcomputed tomography, and ancestral character estimation. All species exhibited pedicel traces arranged in an eustele pattern, with more than ten vascular bundles. Each sepal was supplied by three traces, while petals and stamens received a single trace each, and the gynoecium was vascularized by the remaining bundles. A consistent petal-stamen vascular ring was observed, even in flowers without epipetalous stamens, suggesting an evolutionary remnant of a petal-stamen tube. In Asclepias curassavica, staminal traces contributed lateral bundles to the petals. Staminal coronas in Asclepiadoideae varied in their vascularization. Analyses indicated that non-vascularized coronas, the presence of petal vascularization derived from staminal traces, and unbranched stamen traces represent ancestral conditions. These vascular patterns reveal complex evolutionary trajectories and support the hypothesis of an ancestral floral tube. The results contribute to a deeper understanding of structural and evolutionary aspects of floral diversity in Apocynaceae.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"207-220"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual stress (waterlogging and salinity) resilience in Arabidopsis thaliana conferred by overexpression of a putative daylily (Hemerocallis spp.) betaine aldehyde dehydrogenase gene.","authors":"Meiqing Pan, Anqi Zhao, Min Fan, Xiang Liu, Yanpeng Wang, Qiaoping Qin, Dongmei Yin, Di-An Ni","doi":"10.1007/s10265-026-01692-6","DOIUrl":"10.1007/s10265-026-01692-6","url":null,"abstract":"<p><p>Glycine betaine (GB), a key osmoprotectant in plants, alleviates abiotic stress through osmotic adjustment and reactive oxygen species (ROS) scavenging. Although betaine aldehyde dehydrogenase (BADH) catalyzes GB biosynthesis and enhances salinity tolerance in crops such as rice and maize, its role in waterlogging adaptation remains unclear. A putative BADH gene, HfBADH1, was cloned from waterlogging-treated daylily (Hemerocallis spp.) 'Autumn Red'. HfBADH1 expression increased 6.8-fold under waterlogging and 8.9-fold under salinity. Notably, HfBADH1 expression was repressed by abscisic acid (ABA), in contrast with typical stress-responsive genes. In addition, the activities of BADH and aldehyde dehydrogenase (ALDH) correlated with waterlogging tolerance among different daylily cultivars. Overexpression of HfBADH1 in Arabidopsis thaliana under the CaMV35S promoter resulted in 2.4-fold higher BADH activity and twofold higher ALDH activity than in wild-type plants. The transgenic lines exhibited enhanced tolerance to both salinity and waterlogging. Stress responses were evaluated by antioxidant and anaerobic respiration enzyme activities, and physiological indices including malondialdehyde (MDA) levels and chlorophyll content. The observed stress tolerance in transgenic A.thaliana suggested that BADH overexpression helps stabilize cellular structures and scavenges ROS. The increase in ALDH activity indicated that HfBADH1 overexpression may facilitate aldehyde oxidation, supporting adaptation to hypoxic conditions induced by waterlogging. This study is the first to link BADH overexpression to dual stress resilience, highlighting its combined enzymatic roles in GB biosynthesis and aldehyde detoxification. The ABA-independent regulatory pattern provides insight into noncanonical stress adaptation and suggests new strategies for engineering crops tolerant to saline and flood-prone environments.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"267-281"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146180482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The combined effects of drought and defoliation on non-structural carbohydrates in different organs of Chinese pine.","authors":"Citian Li, Yihang Zhu, Xiangmin Liang, Xinyi Guo, Changhui Peng, Tong Li, Jiayi Tang, Cong Liu, Xin Li, Xiaolu Zhou, Hanxiong Song, Zelin Liu, Ziying Zou, Xuding Wen","doi":"10.1007/s10265-025-01688-8","DOIUrl":"10.1007/s10265-025-01688-8","url":null,"abstract":"<p><p>Climate change imposes negative impacts on forest ecosystems through both abiotic stressors, such as drought, and biotic stressors, such as insect-induced defoliation. Chinese pine (Pinus tabulaeformis), a primary afforestation species in the northwest region of China, has recently faced increasing threats from drought and defoliation, leading to widespread mortality. This study investigates the combined effects of drought and defoliation on the growth and non-structural carbohydrate (NSC) dynamics of Chinese pine seedlings. A greenhouse-based manipulative experiment lasting 80 days was conducted, incorporating three watering treatments (100% field water-holding capacity, 30-40% field water-holding capacity, and no watering) and two defoliation treatments (defoliated and non-defoliated). Under moderate drought, seedlings exhibited increased concentrations of soluble sugars and starch in leaves and stems, demonstrating an active resistance to drought stress. However, under severe drought, NSC concentrations significantly decreased in all organs. Defoliation exacerbated the negative effects of drought, particularly impairing the NSC reserves in roots, thereby weakening the resilience of seedlings. The combination between drought and defoliation significantly altered the allocation patterns of soluble sugars and starch among roots, stems, and leaves in Chinese pine, highlighting the critical role of NSC in plant responses to environmental stressors. This study emphasizes the need to consider both drought and defoliation challenges simultaneously in ecological restoration practices to ensure the resilience of forest ecosystems.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"181-193"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced lipidomic techniques for high-throughput profiling of complex sphingolipids in plant tissues.","authors":"Toshiki Ishikawa","doi":"10.1007/s10265-025-01687-9","DOIUrl":"10.1007/s10265-025-01687-9","url":null,"abstract":"<p><p>Sphingolipids are essential membrane components that regulate various cellular functions by forming ordered domains. The structural core of sphingolipids comprises a long-chain sphingoid base and its N-acylated ceramide, modified with diverse head groups to form complex sphingolipids. In plants, sphingolipid structures exhibit unique structural diversity in both the ceramide moiety and head group. The complicated sphingolipid structures suggest unique functions, but complicate lipidomic studies. A new methodology was established for simple sample preparation and targeted mass spectrometry, to advance high-throughput techniques for plant sphingolipidomics. A single-tube extraction protocol, consisting of the sequential procedures of enzyme inactivation, alkaline saponification and acidic 1-butanol phase partitioning, was optimized to enable rapid (~ 2.5 h) preparation of all sphingolipid classes with improved recovery. Over 900 molecular species from a theoretical plant sphingolipidome library were separated via reverse-phase liquid chromatography and quantified in a single run using scheduled monitoring of targeted mass spectrometry. This method enables large-scale profiling of the natural distribution of sphingolipids across diverse plant materials.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"309-316"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12992377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic defects in the CDP-choline pathway for phosphatidylcholine biosynthesis cannot be transmitted to offspring via male gametophytes owing to interruption of autophagy-like processes required for pollen germination in Arabidopsis thaliana.","authors":"Momoka Wada, Chiaki Kuga, Kimie Atsuzawa, Atsuko Miyagi, Toshiki Ishikawa, Masatoshi Yamaguchi, Yuki Fujiki, Yasuko Kaneko, Maki Kawai-Yamada, Ikuo Nishida","doi":"10.1007/s10265-025-01690-0","DOIUrl":"10.1007/s10265-025-01690-0","url":null,"abstract":"<p><p>Phosphatidylcholine is a major plant membrane phospholipid that contributes to the biogenesis and desaturation of membrane lipids and storage lipids. Thus, to ensure reproductive capacity, any genetic defect that affects phosphatidylcholine biosynthesis must be eliminated before fertilization. In Arabidopsis thaliana, phosphatidylcholine biosynthesis depends on CCT1 and CCT2, both encoding CTP: phosphorylcholine cytidylyltransferase. Using A. thaliana T-DNA-tagged mutants, we demonstrate that neither cct1-3 cct2-3 nor cct1-3 cct2-5 seedlings are viable. Reciprocal crosses of cct2-3/CCT2 or cct2-5/CCT2 plants in the cct1-3 background revealed that neither cct2-3 nor cct2-5 was transmitted via cct1-3 male gametophytes, although each allele was transmitted via cct1-3 female gametophytes. Although all pollen grains on a pollen quartet from qrt1-1 cct1-3 cct2-5/CCT2 plants were viable, none of the cct1-3 cct2-5 pollen grains from cct2-5 cct1-3/CCT1 and cct1-3 cct2-5/CCT2 plants were able to germinate in vitro. Transmission electron microscopy analysis of pollen grains subjected to pollen germination revealed that cct1-3 cct2-5 pollen grains developed unusual ultrafine structures, such as lipid bodies of disproportionate size including extremely enlarged ones, swelling of small granular structures, inhibition of vacuole development, and accumulation of incomplete autophagosome-like bodies enclosing various intracellular compartments. Thus, transmission of cct1-3 cct2-5 to offspring via male gametophytes appears to be strictly prohibited by interruption of autophagic processes required for pollen germination, thereby preventing the widespread dispersal of deleterious mutations among the progeny. By contrast, cct1-3 cct2-5 was partly transmissible via female gametophytes, so the background genome could be rescued by fertilization.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"233-253"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12992434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular docking of the DREB(A-6) group and enhanced thermotolerance in potato by overexpressing the DREB(A-6) gene.","authors":"Saba Azeem, Faiza Munir, Rabia Amir, Alvina Gul","doi":"10.1007/s10265-026-01694-4","DOIUrl":"10.1007/s10265-026-01694-4","url":null,"abstract":"<p><p>Dehydration-responsive element binding (DREB) transcription factors (TFs) play an indispensable role in abiotic stress tolerance, including heat stress via abscisic acid (ABA) dependent and independent pathways. Among them, the DREB (A-6) subgroup remains least characterized, and the role of StDREB30 (A-6) under heat stress, particularly in association with ABA signaling, is not previously explored. This study aimed to characterize StDREB (A-6) group by molecular modelling and explore the role of StDREB30 in thermotolerance and its association with exogenous ABA. Molecular modelling was performed to predict 3D structures using SWISS-MODEL. STRING database was employed for protein-protein interactions (PPI), and molecular docking analysis was performed using the HDOCK server. Further, the role of StDREB30 (A-6) was assessed under heat (42 °C), ABA (50 µM), and combined (heat + ABA) treatments in potato. Overexpression of StDREB30 enhanced the tolerance in transgenic (T) potato under combined treatment of heat and ABA as compared to wild type (WT) counterparts. StDREB30 overexpressing plants showed significant upregulation of the StDREB30 gene, improved phenotypic traits and physiological features under heat and combined heat + ABA treatment. Transgenic lines maintained higher relative water content (i.e., 70% in T vs. 59% in WT), low malondialdehyde (3.1 nmol/g FW in T vs. 3.9 nmol/g FW in WT) and high anthocyanin content. It also improved photosynthetic parameters, including the maximum quantum yield of photosystem II (0.7 in T vs. 0.6 in WT), coefficient of photochemical quenching (0.6 in T vs. 0.49 in WT), and chlorophyll meter (SPAD) values (45 in T vs. 41 in WT). Moreover, RT-PCR analysis confirmed strong up-regulation of StDREB30 (i.e., 5.8, 6.1, and 7.2-fold in leaves, shoots and roots, respectively) under combined heat + ABA treatment compared to non-transgenic counterparts. The transgenic plants showed higher antioxidant activities, decreased reactive oxygen species, and cell death. Additionally, the combined (heat + ABA) treatment enhanced tuberization in transgenic plants. It is proposed that ABA primarily serves as a stress signal modulator without significantly influencing the physiological, biochemical and expression patterns under non-stressed conditions but significantly alleviates the effects of heat stress in combined treatment.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":"283-308"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146180544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclic nucleotide-gated channels serve as core components for Ca²⁺ signal encoding in guard cells.","authors":"Yan-Qiu Tan, Yong-Fei Wang","doi":"10.1007/s10265-026-01696-2","DOIUrl":"https://doi.org/10.1007/s10265-026-01696-2","url":null,"abstract":"<p><p>Tempo-spatial intracellular Ca²⁺ changes/oscillations mainly in cytoplasm and nucleoplasm are the primary mechanisms for the encoding and transmission of upstream stimulating signals to corresponding downstream responses, and the cytosolic Ca²⁺ signals are involved in numerous biological processes. External Ca²⁺ influx mediated by Ca²⁺ channels in the plasma membrane (PM) and the Ca²⁺ release from intracellular Ca²⁺ stores, such as vacuoles and endoplasmic reticulum (ER), respectively contribute to the majority and minority of Ca²⁺ for the generation of cytosolic Ca²⁺ signals, and the dynamic changes of PM Ca²⁺ channel activity directly control the rhythms of external Ca²⁺ influx and the subsequent cytosolic Ca²⁺ oscillations. Thus, the PM Ca²⁺ channels are the core components for Ca²⁺ signal encoding, but remain to be addressed for decades. Stomatal guard cell is an ideal plant cell model for deciphering Ca²⁺ signaling in plants, and more and more studies revealed that cyclic nucleotide-gated channels (CNGCs) play essential roles in Ca²⁺ signal encoding in guard cells. This minireview briefly summarizes the main recent advances in this field, highlights the core roles of CNGCs and underlying mechanism in guard cells, and discusses the possible remaining scientific questions and perspectives.</p>","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147307154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Year's Greeting 2026 from the Journal of Plant Research.","authors":"Noriko Inada","doi":"10.1007/s10265-026-01693-5","DOIUrl":"https://doi.org/10.1007/s10265-026-01693-5","url":null,"abstract":"","PeriodicalId":16813,"journal":{"name":"Journal of Plant Research","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146010827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}