{"title":"Biochemical properties of phytase immobilized and its effect on growth parameters of tomato","authors":"Neslihan Dikbaş, Şeyma Alım, Sevda Uçar, Merve Şenol Kotan","doi":"10.1002/jpln.202300311","DOIUrl":"https://doi.org/10.1002/jpln.202300311","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Phosphorus (P) is one of the nonrenewable resources of critical importance in agricultural production. P is present in soil in organic and inorganic forms. Phytate constitutes the majority of organic P in soil. Phytate binds strongly to the solid phase of the soil and becomes unavailable for use by plants. Therefore, the soluble phytate-P ratio in soil is mostly at very low levels. Plants and associated microorganisms secrete organic acids and hydrolyzing enzymes such as phytase to dissolve phytate in the soil. Both the solubility of phytate and phytase activity are limiting properties for the uptake of phytate-P by plants.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Our aim was to evaluate the effects of phytase immobilized on zinc oxide nanoparticles (ZnO Np) on tomato plant (<i>Solanum lycopersicum</i>) growth parameters. In this study, seedling period was analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In the study, phytase activity of 13 different bacteria was investigated, and phytase was purified from <i>Lactobacillus kefiri</i>, showing the highest activity, and its biochemical properties were determined. Phytase was immobilized on zinc oxide (ZnO) nanoparticles and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopes analysis. The effects of ZnONps, immobilized phytase, and free phytase on the growth parameters of tomato plant were investigated. Tomato seeds were soaked with ZnONps, immobilized and free phytase for 30 min at room temperature and sown in pots containing suitable growing medium. Vegetative development of tomato plant, plant height, number of lateral branches, main stem diameter, distance between nodes, number of nodes, main root, and shoot length were determined.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Phytase was partially purified with 7.60% recovery and specific activity of 1758.5 (EU mg<sup>−1</sup> protein). Molecular mass of partially purified phytase was approx.72 kD, optimum pH and temperature values were determined as pH 5.0 and 70–80°C, respectively. Immobilized phytase caused a significant increase of 41.1% in plant height, 64.1% in main root, and 36.1% in shoot length in tomato plants compared to the control. In addition, a significant increase was observed in the number of side branches, main stem diameter, distance between nodes, number of nodes, and vegetative growth of the plant.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 ","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"187 4","pages":"533-544"},"PeriodicalIF":2.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jpln.202300311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141967947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Spermidine mitigates salt stress in grapevine with alterations in physicochemical properties and nutrient composition","authors":"Ghaffar Shokri, Jafar Amiri, Mohsen Barin","doi":"10.1002/jpln.202400003","DOIUrl":"10.1002/jpln.202400003","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Polyamines are plant growth regulators that exert a pivotal role in salt tolerance.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>This research focused on investigating the effect of spermidine on morphological and physicochemical characteristics and ion accumulation of two grapevine cultivars under NaCl stress.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A greenhouse experiment was conducted with three factors, including two grapevine cultivars (<i>Vitis vinifera</i> L. cv. Bidaneh-Sefid and cv. Siah-Sardasht), four levels of NaCl (together with the nutrient solution, including 0 [control], 20, 40, and 80 mM), and four spermidine levels ([foliar spray], 0 [control], 0.25, 0.5, and 1 mM). The experiment was performed in a factorial trial in accordance with a randomized complete design with three replicates.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Vegetative growth indices, including leaf number, fresh and dry weight of shoot, and root, were decreased by NaCl treatments. The application of spermidine positively reduced the effects of NaCl on morphological characteristics. Moreover, NaCl and/or spermidine significantly (<i>p</i> ≤ 0.05) improved antioxidant enzyme activities associated with rising total protein accumulation. NaCl stress significantly decreased ion percentage (calcium, magnesium, phosphate, potassium, iron, and zinc) in the leaves of both cultivars. Based on the results, increasing salinity levels significantly boosted plant Na<sup>+</sup> and Cl<sup>−</sup> percentage, along with increased membrane permeability and malondialdehyde (MDA) concentration. Interestingly, cv. Bidaneh-Sefid leaves accumulated less Na<sup>+</sup> and Cl<sup>−</sup> compared to the other cultivar. On the other hand, applying spermidine reduced the levels of Na<sup>+</sup> and Cl<sup>−</sup> in both cultivars, and this reduction was associated with a decrease in membrane permeability and MDA concentration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The findings confirmed the role of spermidine in reducing the negative effects of NaCl, although more investigations with different grapevine cultivars under NaCl stress are required.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"187 4","pages":"516-532"},"PeriodicalIF":2.6,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141343507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaofeng Gao, Ning He, Shubo Fang, Bolun Zhang, Maoqiu Wang, Peimin He
{"title":"The above and the belowground nitrogen allocation strategy of Scirpus mariqueter based on 15N isotope tracing along an elevation gradient and its significance for coastal wetlands restoration","authors":"Xiaofeng Gao, Ning He, Shubo Fang, Bolun Zhang, Maoqiu Wang, Peimin He","doi":"10.1002/jpln.202400070","DOIUrl":"10.1002/jpln.202400070","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Ecological restoration of coastal wetlands has become particularly urgent worldwide as wetland areas have declined dramatically over the past two decades.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>To understand the nitrogen allocation strategy of <i>Scirpus mariqueter</i> (<i>S. mariqueter</i>) and provide theory support for future wetland management and restoration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The study investigated the response mechanism of <i>S. mariqueter</i> to altitude spatial changes in Nanhui Dongtan from 2017 to 2019 using remote sensing imagery and field surveys. The ecological adaptability of <i>S. mariqueter</i> at different elevations (denoted as A, B, and C for elevations 2.40, 3.15, and 3.49 m, respectively) was analyzed through <sup>15</sup>N stable isotope tracing technology. In July and September 2020, <sup>15</sup>N-enriched urea solution was uniformly sprayed onto the leaf surfaces of <i>S. mariqueter</i> at different sites. Plant samples were collected at the end of July and September, and the aboveground, belowground, seed, and rhizome biomass were measured, followed by <sup>15</sup>N isotope tests.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>(1) From 2017 to 2019, the biomass of <i>S. mariqueter</i> significantly increased in the elevation change of 0.44–0.48 m, with the maximum aboveground biomass increase of 488.70 g dm<sup>−2</sup>. The density also increased significantly in the elevation change of 0.13–0.43 m, peaking at 674.02 plants m<sup>−2</sup>; (2) during the growing period, the biomass of A, B, and C increased. The aboveground portion of the <sup>15</sup>N allocation rate accounted for 74%–84%. The belowground portion of the <sup>15</sup>N allocation rate positively correlated with elevation; (3) during the reproductive period, elevation positively correlated with the <sup>15</sup>N distribution rate of seeds and corms, as well as the biomass allocation rate of seeds and aboveground portions. The <sup>15</sup>N allocation rate of the corms was higher than that of seeds. Additionally, elevation exhibited a negative correlation with belowground biomass allocation rate. (4) Point A has the highest difference of above and belowground biomass proportion, and <sup>15</sup>N isotope allocation. Area of point A is the critical area affecting vegetation expansion and should be paid more attention in the future work of coastal management and restoration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>There is an adaption strategy of <i>S. mariqueter</i> that affect","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"187 4","pages":"504-515"},"PeriodicalIF":2.6,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141368537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Contents: J. Plant Nutr. Soil Sci. 3/2024","authors":"","doi":"10.1002/jpln.202470034","DOIUrl":"https://doi.org/10.1002/jpln.202470034","url":null,"abstract":"","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"187 3","pages":"438"},"PeriodicalIF":2.5,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jpln.202470034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141245857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Johannes L. Jensen, Franca Giannini-Kurina, Jørgen Eriksen
{"title":"Similar root and stubble biomass carbon in grass–clover leys irrespective of yield, species composition, sward age, and fertilization","authors":"Johannes L. Jensen, Franca Giannini-Kurina, Jørgen Eriksen","doi":"10.1002/jpln.202400093","DOIUrl":"10.1002/jpln.202400093","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Crop- and site-specific quantification of non-harvestable aboveground residues and root biomass is essential for predicting management-induced changes in soil C storage.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>The aim of this study was to quantify stubble and root biomass C from productive grass–clover leys used for cutting as affected by fertilization and sward age.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Based on an organic long-term dairy crop rotations experiment with 4 years of grass–clover in a six-course rotation, we examined the effects of fertilization (unfertilized and 300 kg total-N ha<sup>−1</sup> in cattle slurry) and sward age (1–4-year-old) on herbage yield and composition, stubble biomass, and composition and root biomass of grass–clover ley.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ley duration and fertilization altered plant community composition and aboveground productivity but did not affect stubble and root biomass C.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The results question the use of yield-dependent allometric functions for grass–clover ley used in simulation models and life cycle assessments for C accounting in agricultural systems. For predictions of soil C changes, we recommend the use of a fixed stubble-derived C input from grass–clover ley regardless of sward age and fertilization-induced differences in species composition, and herbage yield. Likewise, a fixed root-derived C input for 1-year-old grass–clover, irrespective of fertilization, may be implemented. However, the contribution of continuous rhizodeposition and fine root turnover to root-derived C input need to be accounted for.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"187 4","pages":"494-503"},"PeriodicalIF":2.6,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jpln.202400093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141197590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carlos Felipe dos Santos Cordeiro, Fábio Rafael Echer, Ciro Antonio Rosolem
{"title":"Root systems of peanut cultivars respond differently to soil P availability to improve P uptake","authors":"Carlos Felipe dos Santos Cordeiro, Fábio Rafael Echer, Ciro Antonio Rosolem","doi":"10.1002/jpln.202300144","DOIUrl":"10.1002/jpln.202300144","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Peanut (<i>Arachis hypogaea</i> L.) is regarded as a crop with high nutrient use efficiency, but there may be differences between cultivars. Furthermore, there is little information on the strategy of peanut cultivars to adapt to soil P availability and to what extent they explore non-labile P pools.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Our objective was to evaluate growth, root morphology, enzymatic activity in the rhizosphere, and P uptake of peanut cultivars grown under different soil P status.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The study was conducted in a greenhouse in 6-L pots. Soils with low P (without fertilization) and high P content (with fertilization) and seven peanut cultivars of different origins, different maturation groups, and release years were investigated. Peanut shoot yield, phosphorus uptake, root growth, soil P fractions as well as phosphatase activity in the rhizosphere soil were determined.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In P-deficient soil, a higher dry matter yield was associated with longer root hairs and root length, which resulted in decreased soil non-labile P was observed mainly with cultivars developed in Argentina (ARG-medium-old and ARG-medium-new) and the late maturity Brazilian cultivar (BR I-late new). These cultivars adapted well to P deficiency and were less dependent on labile P. New Brazilian early and medium maturity cultivars developed less, shorter root hairs, and showed low acid phosphatase activity in the rhizosphere under P deficiency, resulting in lower P uptake and dry matter yield. Under high P availability, new Brazilian cultivars of medium and late maturity showed the highest dry matter yield (9.0 and 9.8 g plant<sup>−1</sup>, respectively) and longest roots, around 120 m plant<sup>−1</sup>. High P availability decreased root hairs in all cultivars.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Overall, the adaptation of peanut cultivars to P-deficient soils was lower for the new mid- and early-maturing Brazilian cultivars compared with the Argentinian and old or late-maturing Brazilian cultivars. The main strategies of P-efficient cultivars under low P availability are to increase root length, root hair length, and root hair density.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"187 4","pages":"484-493"},"PeriodicalIF":2.6,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141110294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of simultaneous increase in CO2 and temperature on soil aggregates, associated organic carbon, and nutritional quality of rice–wheat grains","authors":"Karnena Koteswara Rao, Sharad Kumar Dwivedi, Santosh Kumar, Saubhagya Kumar Samal, N. Raju Singh, Janki Sharan Mishra, Ved Prakash, Anup Kumar Choubey, Manoj Kumar, Bhagwati Prasad Bhatt","doi":"10.1002/jpln.202200261","DOIUrl":"10.1002/jpln.202200261","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Food and nutritional security remain a major thrust area in the under developed and developing countries. These problems are exaggerated by the unprecedented challenges of climate change.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>The aim of this study was to assess the impact of climate change on grain quality of wheat and rice genotypes as well as their effect on soil aggregate fractions and aggregate associated carbon.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>In the context, the present study was formulated by considering four predicted climate scenarios, namely, T<sub>0</sub>C<sub>0</sub> (ambient condition), T<sub>0</sub>C<sub>1</sub> (approx. 25% higher CO<sub>2</sub>), T<sub>1</sub>C<sub>0</sub> (2°C higher temperature) and T<sub>1</sub>C<sub>1</sub> (25% higher CO<sub>2</sub> + 2°C higher temperature) and their impact on grain quality of wheat (HD2967, HD2733, DBW17, and HD3093) and rice (IR83376-B-B-24-2, IR84895-B-127-CRA-5-1-1, R Bhagwati, and IR64) genotypes as well as soil aggregate fractions and aggregate associated carbon.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The result revealed that T<sub>0</sub>C<sub>1</sub> has a negative impact on grain nitrogen and protein content. On an average, nitrogen content in wheat and rice showed a decrease of about 15.55% (5.52%–25.32%) and 11.44% (3.33%–23.86%), respectively. Interestingly, the concurrent effect of elevated CO<sub>2</sub> and temperature resulted in higher nitrogen and protein content as compared to other climate conditions. Further, P (P) content in the wheat and rice grains also improved under the elevated CO<sub>2</sub> condition, whereas the content of potassium was not significantly influenced. Apart from major nutrients, micronutrients (Zn and Fe) were significantly influenced by climatic variables. The study revealed that grain Zn and Fe content of both the crops were reduced due to elevated CO<sub>2</sub>. The data on soil aggregate fractions revealed that elevated CO<sub>2</sub> favors the formation of macro-aggregate, whereas an increase in temperature favors micro-aggregate fractions in the soil. Further, the elevation of CO<sub>2</sub> also resulted in the accumulation of more carbon in the macro-aggregates.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>We conclude that elevated CO<sub>2</sub> and temperature cause specific changes in soil aggregate formation and grain nutrient quality. Based on molar ratio of P/Zn and P/Fe, we identified varieties of rice (IR83376-B-B-24-2) and wheat (HD2733) with higher bioavailabi","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"187 4","pages":"470-483"},"PeriodicalIF":2.6,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140934865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}