{"title":"固定植酸酶的生化特性及其对番茄生长参数的影响","authors":"Neslihan Dikbaş, Şeyma Alım, Sevda Uçar, Merve Şenol Kotan","doi":"10.1002/jpln.202300311","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Phosphorus (P) is one of the nonrenewable resources of critical importance in agricultural production. P is present in soil in organic and inorganic forms. Phytate constitutes the majority of organic P in soil. Phytate binds strongly to the solid phase of the soil and becomes unavailable for use by plants. Therefore, the soluble phytate-P ratio in soil is mostly at very low levels. Plants and associated microorganisms secrete organic acids and hydrolyzing enzymes such as phytase to dissolve phytate in the soil. Both the solubility of phytate and phytase activity are limiting properties for the uptake of phytate-P by plants.</p>\n </section>\n \n <section>\n \n <h3> Aims</h3>\n \n <p>Our aim was to evaluate the effects of phytase immobilized on zinc oxide nanoparticles (ZnO Np) on tomato plant (<i>Solanum lycopersicum</i>) growth parameters. In this study, seedling period was analyzed.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In the study, phytase activity of 13 different bacteria was investigated, and phytase was purified from <i>Lactobacillus kefiri</i>, showing the highest activity, and its biochemical properties were determined. Phytase was immobilized on zinc oxide (ZnO) nanoparticles and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopes analysis. The effects of ZnONps, immobilized phytase, and free phytase on the growth parameters of tomato plant were investigated. Tomato seeds were soaked with ZnONps, immobilized and free phytase for 30 min at room temperature and sown in pots containing suitable growing medium. Vegetative development of tomato plant, plant height, number of lateral branches, main stem diameter, distance between nodes, number of nodes, main root, and shoot length were determined.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Phytase was partially purified with 7.60% recovery and specific activity of 1758.5 (EU mg<sup>−1</sup> protein). Molecular mass of partially purified phytase was approx.72 kD, optimum pH and temperature values were determined as pH 5.0 and 70–80°C, respectively. Immobilized phytase caused a significant increase of 41.1% in plant height, 64.1% in main root, and 36.1% in shoot length in tomato plants compared to the control. In addition, a significant increase was observed in the number of side branches, main stem diameter, distance between nodes, number of nodes, and vegetative growth of the plant.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The results showed that the immobilized phytase enzyme has a positive effect on seedling growth in tomato and can be used in tomato cultivation in the future.</p>\n </section>\n </div>","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"187 4","pages":"533-544"},"PeriodicalIF":2.6000,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jpln.202300311","citationCount":"0","resultStr":"{\"title\":\"Biochemical properties of phytase immobilized and its effect on growth parameters of tomato\",\"authors\":\"Neslihan Dikbaş, Şeyma Alım, Sevda Uçar, Merve Şenol Kotan\",\"doi\":\"10.1002/jpln.202300311\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Phosphorus (P) is one of the nonrenewable resources of critical importance in agricultural production. P is present in soil in organic and inorganic forms. Phytate constitutes the majority of organic P in soil. Phytate binds strongly to the solid phase of the soil and becomes unavailable for use by plants. Therefore, the soluble phytate-P ratio in soil is mostly at very low levels. Plants and associated microorganisms secrete organic acids and hydrolyzing enzymes such as phytase to dissolve phytate in the soil. Both the solubility of phytate and phytase activity are limiting properties for the uptake of phytate-P by plants.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Aims</h3>\\n \\n <p>Our aim was to evaluate the effects of phytase immobilized on zinc oxide nanoparticles (ZnO Np) on tomato plant (<i>Solanum lycopersicum</i>) growth parameters. In this study, seedling period was analyzed.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>In the study, phytase activity of 13 different bacteria was investigated, and phytase was purified from <i>Lactobacillus kefiri</i>, showing the highest activity, and its biochemical properties were determined. Phytase was immobilized on zinc oxide (ZnO) nanoparticles and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopes analysis. The effects of ZnONps, immobilized phytase, and free phytase on the growth parameters of tomato plant were investigated. Tomato seeds were soaked with ZnONps, immobilized and free phytase for 30 min at room temperature and sown in pots containing suitable growing medium. Vegetative development of tomato plant, plant height, number of lateral branches, main stem diameter, distance between nodes, number of nodes, main root, and shoot length were determined.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Phytase was partially purified with 7.60% recovery and specific activity of 1758.5 (EU mg<sup>−1</sup> protein). Molecular mass of partially purified phytase was approx.72 kD, optimum pH and temperature values were determined as pH 5.0 and 70–80°C, respectively. Immobilized phytase caused a significant increase of 41.1% in plant height, 64.1% in main root, and 36.1% in shoot length in tomato plants compared to the control. In addition, a significant increase was observed in the number of side branches, main stem diameter, distance between nodes, number of nodes, and vegetative growth of the plant.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>The results showed that the immobilized phytase enzyme has a positive effect on seedling growth in tomato and can be used in tomato cultivation in the future.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16802,\"journal\":{\"name\":\"Journal of Plant Nutrition and Soil Science\",\"volume\":\"187 4\",\"pages\":\"533-544\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-06-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jpln.202300311\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Nutrition and Soil Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jpln.202300311\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Nutrition and Soil Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jpln.202300311","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Biochemical properties of phytase immobilized and its effect on growth parameters of tomato
Background
Phosphorus (P) is one of the nonrenewable resources of critical importance in agricultural production. P is present in soil in organic and inorganic forms. Phytate constitutes the majority of organic P in soil. Phytate binds strongly to the solid phase of the soil and becomes unavailable for use by plants. Therefore, the soluble phytate-P ratio in soil is mostly at very low levels. Plants and associated microorganisms secrete organic acids and hydrolyzing enzymes such as phytase to dissolve phytate in the soil. Both the solubility of phytate and phytase activity are limiting properties for the uptake of phytate-P by plants.
Aims
Our aim was to evaluate the effects of phytase immobilized on zinc oxide nanoparticles (ZnO Np) on tomato plant (Solanum lycopersicum) growth parameters. In this study, seedling period was analyzed.
Methods
In the study, phytase activity of 13 different bacteria was investigated, and phytase was purified from Lactobacillus kefiri, showing the highest activity, and its biochemical properties were determined. Phytase was immobilized on zinc oxide (ZnO) nanoparticles and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopes analysis. The effects of ZnONps, immobilized phytase, and free phytase on the growth parameters of tomato plant were investigated. Tomato seeds were soaked with ZnONps, immobilized and free phytase for 30 min at room temperature and sown in pots containing suitable growing medium. Vegetative development of tomato plant, plant height, number of lateral branches, main stem diameter, distance between nodes, number of nodes, main root, and shoot length were determined.
Results
Phytase was partially purified with 7.60% recovery and specific activity of 1758.5 (EU mg−1 protein). Molecular mass of partially purified phytase was approx.72 kD, optimum pH and temperature values were determined as pH 5.0 and 70–80°C, respectively. Immobilized phytase caused a significant increase of 41.1% in plant height, 64.1% in main root, and 36.1% in shoot length in tomato plants compared to the control. In addition, a significant increase was observed in the number of side branches, main stem diameter, distance between nodes, number of nodes, and vegetative growth of the plant.
Conclusions
The results showed that the immobilized phytase enzyme has a positive effect on seedling growth in tomato and can be used in tomato cultivation in the future.
期刊介绍：
Established in 1922, the Journal of Plant Nutrition and Soil Science (JPNSS) is an international peer-reviewed journal devoted to cover the entire spectrum of plant nutrition and soil science from different scale units, e.g. agroecosystem to natural systems. With its wide scope and focus on soil-plant interactions, JPNSS is one of the leading journals on this topic. Articles in JPNSS include reviews, high-standard original papers, and short communications and represent challenging research of international significance. The Journal of Plant Nutrition and Soil Science is one of the world’s oldest journals. You can trust in a peer-reviewed journal that has been established in the plant and soil science community for almost 100 years.
Journal of Plant Nutrition and Soil Science (ISSN 1436-8730) is published in six volumes per year, by the German Societies of Plant Nutrition (DGP) and Soil Science (DBG). Furthermore, the Journal of Plant Nutrition and Soil Science (JPNSS) is a Cooperating Journal of the International Union of Soil Science (IUSS). The journal is produced by Wiley-VCH.
Topical Divisions of the Journal of Plant Nutrition and Soil Science that are receiving increasing attention are:
JPNSS – Topical Divisions
Special timely focus in interdisciplinarity:
- sustainability & critical zone science.
Soil-Plant Interactions:
- rhizosphere science & soil ecology
- pollutant cycling & plant-soil protection
- land use & climate change.
Soil Science:
- soil chemistry & soil physics
- soil biology & biogeochemistry
- soil genesis & mineralogy.
Plant Nutrition:
- plant nutritional physiology
- nutrient dynamics & soil fertility
- ecophysiological aspects of plant nutrition.
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