Journal of Pharmaceutical Negative Results最新文献

{"title":"Formulation And In Vitro Evaluation Of Fluconazole Niosomal Gel For Topical Drug Delivery","authors":"","doi":"10.47750/pnr.2022.13.s08.498","DOIUrl":"https://doi.org/10.47750/pnr.2022.13.s08.498","url":null,"abstract":"Fluconazole, a macrolide antibiotic, is employed to treat certain susceptible bacteria. Niosomes play a significant role in drug delivery since they can alter pharmacokinetics and bioavailability characteristics, as well as minimise toxicity. Niosomes are becoming more and more crucial in the administration of drugs. While decreasing the drug's systemic absorption, topically administered niosomes can lengthen the duration that medications remain in the stratum corneum and epidermis. Fluconazole-loaded topical gel niosomes are intended to be developed and evaluated in this study. Span 20, 40, 60 (as a non-ionic surfactant) and cholesterol were used to create niosomes by the thin film hydration method (as stable vesicle forming agent). Different dosages of the drugs, surfactants, and cholesterol were used to make niosomes (0.30:1:0.6, 0.6:1:0.6, 0.9:1:0.6). The vesicle size, surface shape, % entrapment effectiveness, drug content, and in vitro drug release of the niosomal dispersion were examined. Using a UV spectrophotometer, the drug concentration and entrapment efficiency were determined at 262 nm. A range of 77.650.25 to 94.120.48 was discovered for the entrapment efficiency. A maximum entrapment efficiency of 94.120.48 was shown for Formulation FS5, which contains Span 60, and 93.900.70 was shown for Formulation FT4, which contains Tween 60. Carbopol 940, glycerol, triethanolamine, and distilled water were used to make fluconazole niosomal gel. Niosomal gel's evaluation was based on its outward appearance, pH, viscosity, drug content, entrapment effectiveness, and in-vitro permeation investigations. The amount of medication released from the niosomal gel was discovered to be 80.76%. The aforementioned data show that encapsulating a fluconazole-loaded niosomal topical gel lengthens drug release, increases drug retention in skin, and enhances cellular permeability.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42297800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphism Of Virulence Genes Of Enterococcus Faecalis Associated With Aerobic Vaginitis","authors":"lbtisam H. Al-Azawi, Anmar H. H. AlFuadi, Wisam S. Abbood","doi":"10.47750/pnr.2023.14.03.424","DOIUrl":"https://doi.org/10.47750/pnr.2023.14.03.424","url":null,"abstract":"The aim of the current study is to determine the virulence genes of E. faecalis and polymorphism in these bacteria. A total of 300 females  who visited the outpatient clinics in the Maternity and Pediatrics Teaching Hospital in Al-Diwaniya city, Iraq were enrolled. For twenty isolates of E. faecalis that showed dense growth when cultured on the specified culture media, then the sensitivity to antibiotics was tested, and then the virulence genes were determined for each isolate, where the results were shown there are statistically significant differences (P<0.05) in the presence of virulence genes in the tested bacterial isolates. (where the genetic analysis by PCR showed the presence of all virulence genes, except for isolate 3 of the gene esp (Enterococcal surface production protein), and isolates 11, 12, 15, 16 and 19 of the hly gene (haemolysin production). ERlC results showed the most of the isolates share a DNA segment of length 1500, 1000 bp, and 2000 pb. The curve also shows that most of the isolates lack the presence of the segment of length 500 pb. As for the pieces of 700, 600 and 400 bp, they showed a very high heterogeneity between the isolates. ln conclusion, most genes responsible for virulence factors were positive, except for hly, which showed positive results for nearly more than half of the isolates, that the virulence factors of said bacteria are a major focus of infection in said bacteria, and if these genes are not available, they are unable to hit or stick to the surface of the vagina. There were two types of genetic polymorphism in strains, one of which is caused by horizontal transmission between strains via plasmids and other horizontal transmission methods, and the other is caused by vertical transmission through mutations that occur between generations, causing high resistance to some antibiotics. vitality.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45777854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction, Isolation Of Chemical Compounds And Evaluation Of Wound Healing Activity Of Bombax Ceiba Plant","authors":"S. Ramasamy, Vinod Kumar Singh","doi":"10.47750/pnr.2022.13.s08.653","DOIUrl":"https://doi.org/10.47750/pnr.2022.13.s08.653","url":null,"abstract":"Herbal plants have a lot of benefits over conventional drugs, including the fact that they usually have less frequent side-effects and might be non-toxic if used for a long time. B. Ceiba is an enormous, stunning, and deciduous tree and native for Northern Australian origin. The present study was based on the extraction, isolation of chemical compounds and evaluation of wound healing activity of Bombax ceiba plant. The chemicals and instruments were obtained from the certified manufacturer and suppliers only. B. ceiba roots and flowers were collected and authenticated from a Botanist at MJPRU Bareilly. The extraction process was done by maceration process. Pharmacological (wound healing) potential was evaluated by wound contraction, re-epithelization, and histopathology. The n-hexane, chloroform, ethyl acetoacetate and methanol extracts of B. ceiba were examined for its phytoconstituents. Wistar rats weighing 130-210g were obtained from the Dept. of Pharmacy, MJP Rohilkhand University Bareilly. The % yield was obtained as 59% for n-hexane, 62% for chloroform, 58% for ethyl acetoacetate and 65% for methanol. According to the preliminary phytochemical analysis, the methanolic B. ceiba extracts showed excellent phytoconstituents of alkaloids, flavonoids, glycosides, carbohydrates, etc. when compared with other solvents extracts. In results, all the groups of animals showed an excellent wound healing activity when compared with control group after 15th day of exposure with treatments. The B. ceiba N-hexane, chloroform, ethyl acetoacetate, and methanol demonstrated rats showed the re-epithelization period of 11.20±0.62**, 12.17±1.30***, 9.3±1.23** and 10.4±1.12***, respectively. It is concluded that each of these has considerably exhibited wound healing potential. It suggests, to isolate the active constituent responsible for the activity.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70924441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug Utilization Of Anti-Diabetic And Anti-Hypertension Drugs At Tertiary Care Teaching Hospital, Bangalore, India","authors":"Mohammadhadi Mosallanezhad","doi":"10.47750/pnr.2023.14.03.416","DOIUrl":"https://doi.org/10.47750/pnr.2023.14.03.416","url":null,"abstract":"Background: Hypertension is extremely prevalent in patients with diabetes. Limited data exist on utilization patterns of antihypertensive in this population are consistent with evidence-based practice guidelines. \u0000Objective: The present study is aimed to evaluate utilization patterns of antihypertensive agents among hypertensive diabetic patients. \u0000Methodology: The study was conducted in the patient pharmacy department of Bangalore. \u0000A prospective observational study was conducted for a period of 6 months in at Tertiary Care Teaching Hospital, Bangalore, India. We included 150 hypertensive diabetic patients with or without other comorbid conditions and patients aged below 18 years and pregnant women were excluded. All the prescribed medications along with other medications and relevant information will be noted in a customized data collection. The study patients will be followed daily until their discharge. The Micromedex, Medscape, articles and relevant references books will be used as tools to review the collected data. The prescribed medication will be checked for their existence in the hospital and also the reelevating calculation. Check for any error in prescription such as doses, frequency and route of administration and analysis. \u0000Result: Out of 150 patients, 50% were male and 50% were female. Maximum patients belonged to age group of 51-60 years. Chronic Kidney Disease (18%) the was the most common associated disease with hypertension and diabetes mellitus. More than half of the patients had received one antihypertensive drug (60%), followed by two (28%), three (10%) and four (2%) antihypertensive drugs. Amlodipine (CCB) was the most commonly prescribed antihypertensive drug (64%). \u0000Conclusion: Amlodipine and furosemide were the most frequently prescribed antihypertensive drugs. Prescription of antihypertensive drugs for some patients with compelling indications were not in congruent with JNC 8 guidelines. This study highlights some therapeutic rationality in this health center. However, targeted education of the prescription-givers and dissemination of treatment guideline could facilitate rational use of drugs and adherence to treatment guide lines.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48217508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect Of Interaction Between Hyper-Video Navigation Tools And The Cognitive Style In Raising The Level Of Learning Rate Of Students At The College Of Education","authors":"Dr. Sultan Saad Albugami","doi":"10.47750/pnr.2023.14.03.414","DOIUrl":"https://doi.org/10.47750/pnr.2023.14.03.414","url":null,"abstract":"The purpose of this study is to determine the effect of the interaction between hyper-video navigation tools (buttons, cognitive maps, and hot spots) and cognitive style (independent of cognitive field / dependent according to the cognitive field) ا on the learning rate of the students at the Department of Education Technology. An experimental approach was used to compare six experimental groups (3*2). The research sample consisted of sixty sixth-grade students from the Department of Learning Techniques, College of Education, University of Jeddah. An embedded figure test (Embedded Figure Test) was used for e-learning technology to determine who is independent of the cognitive field and who is dependent according to the cognitive field. The research sample was divided into six experimental groups, each with ten students. An electronic achievement test was created to measure the learning rate of students. The results showed that the buttons were the appropriate navigation tool for both the independents and the dependents in the cognitive field. That is because the buttons take into account the individual differences between the students, it was concluded that it is the most appropriate tool for roaming through the hyper-video.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42641189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Analysis Of Socio-Cultural Factors Affecting Gender Inequality With A Role In Mental Health","authors":"None Melika keyghobadi","doi":"10.47750/pnr.2023.14.03.415","DOIUrl":"https://doi.org/10.47750/pnr.2023.14.03.415","url":null,"abstract":"Women's needs and expectations in society are changing and evolving now as a result of efforts to reclaim their lost rights and achieve gender justice. In order to understand the cultural-social aspects that contribute to gender disparity, the current research has looked into factors including patriarchal ideology, gender stereotypes, gender socialization, and others. Feminists' conceptions of gender inequality, and Bourdieu's theory have all been used in this study. 385 individuals were chosen as a sample from the married women in Isfahan city who are the statistical population for this study and range in age from 20 to 49. Quota sampling is the technique used in this study. The research's findings show that there is a high level of gender disparity, especially in the economic and cultural spheres, and that there is no correlation between gender stereotypes and inequality, adherence to the patriarchal ideology, or societal norms and inequality. Inequality and gender tolerance exist in the connection.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":"209 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136195815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Efficient EEG Channels-Selection Approaches For Epilepsy Seizure Prediction","authors":"Sidaoui Boutkhil, Sadouni Kadour","doi":"10.47750/pnr.2023.14.03.406","DOIUrl":"https://doi.org/10.47750/pnr.2023.14.03.406","url":null,"abstract":"In this study, we are interested in the epilepsy seizures problem. Indeed, we used binary SVM to predict the ongoing seizures and multiclass SVM to predict different states of patients' epilepsy. Brain activity is used as an efficient source for predicting seizures, it's recorded in Electroencephalography (EEG) segments signal. We propose and compare in this paper, three ideas select channels: the highest frequency channels, the channels of the left part of the head, and the channels of the right part of the head. A features extraction stage is important to produce a rich and relevant dataset, in effect, 22 features are calculated for each segment of 5 min from EEG signal. A binary SVM is used to predict the ongoing seizures named pre-ictal, and a one-versus-all multi-class SVM is used to predict four classes (pre-ictal, ictal, inter-ictal, and post-ictal). A classification rate toward 97%, on the selected channels corpus, was achieved by SVM (binary and multiclass) with the majority of patients.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41625766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Model To Detect Breast Cancer Based On Patient Symptoms","authors":"Mr. Satish Dekka, Dr.K. Narasimha Raju, Dr. D. ManendraSai, Mr. Mohammad Rafi","doi":"10.47750/pnr.2022.13.s05.439","DOIUrl":"https://doi.org/10.47750/pnr.2022.13.s05.439","url":null,"abstract":"The number of medical data warehouses is expanding quickly these days. As a result, it is difficult for us to predict or analyse these facts in order to uncover hidden knowledge that is valuable. For forecasting medical analysis, many machine learning methods and tools are employed. The most prevalent and well-known malignancy, particularly among women, is breast cancer. It ranks among the leading global causes of death. The sole remedy is early detection, which lowers the mortality rate from breast cancer. Breast cells can develop into cancer, which is known as breast cancer. Breast cancer has recently become a highly serious disease, not just in India but also in other nations. The primary goal of this research is to diagnose breast cancer patients as early as possible. Three machine learning approaches Decision Tree, Support Vector Machine, and Logistic Regression are employed for the early detection and prevention of breast cancer patients. These techniques help reduce waiting times and human and technical errors in breast cancer diagnosis. By employing these methods, we can increase the number of lives saved and decrease the death rate by maximising early diagnosis of breast cancer. The likelihood that an infection will be successfully treated depends on precisely identifying and locating it as soon as possible using logistic regression and SVM. A significant obstacle to the diagnosis of breast cancer is the classification of the appropriate machine learning technique. Thus, in order to analyse risk levels that contribute to prognosis, we developed a model for a breast cancer early prediction system. Doctors can diagnose breast cancer using this experimental study, and patients can benefit from early therapy to prolong their lives.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48119771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subtractive Genomics Based Analysis For In Silico Recognition And Characterization Of New Drug Targets In Yersinia Pestis","authors":"Faisal A Al Zahrani, Alam Jamal","doi":"10.47750/pnr.2023.14.03.372","DOIUrl":"https://doi.org/10.47750/pnr.2023.14.03.372","url":null,"abstract":"Yersinia pestis (Y. pestis) is a gram-negative bacteriam.it cause plague disease. Between 30% and 100% of untreated patients die, with pneumonic plague being the deadliest. Chloramphenicol Streptomycin and tetracycline are the three main antibiotics that are used for plague treatment. Even if antibiotics are given right away, 16% of people with bubonic plague and more than 50% of people with pneumonic plague die. The number of plague cases and epidemics over the past 15 years has increased to the point where the disease is now considered reemerging. Researchers have discovered that some strains of the plague are immune to the most effective medications now in use to treat the disease. Y. pestis is worrisome since there is no effective vaccination, it must be treated with antibiotics, and it has a history of being used as a bioweapon. Antibiotic-resistant bacteria are a worldwide problem. It has been revealed that two strains of Y. pestis are highly resistant to eight antimicrobial medicines used for cure. Additionally, it is resistant to a number of commonly used antibiotics, including ampicillin, kanamycin, and spectinomycin. In subtractive genomics approach the bacterial total proteome is gently nethermost to a few numbers of probable targets for drug. The steps which are applied in this method to detect  targets proteins which are non-similar to human and are vital for bacteria and contribution of the sorted out proteins in bacterial pathways of metabolism that are vital for continuity of pathogen. In this study the approach of subtractive genomics is applied on proteins of Y. pestis   and culminated with 4 proteins that may be powerful drug targets and novel zestful molecules can be designed against them to cure the bacteria associated infection.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48234603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Prospective Study On Corticosteroids Utilization Pattern In Dermatology Department Of A Tertiary Care Teaching Hospital, Bangalore, India","authors":"Mehrangiz Nikzad, Sharath Kumar","doi":"10.47750/pnr.2023.14.03.370","DOIUrl":"https://doi.org/10.47750/pnr.2023.14.03.370","url":null,"abstract":"Corticosteroids are widely prescribed in dermatology department and a rational prescribing lead to maximum benefit and minimum untoward effects. The objectives of this study were to assess the corticosteroid prescribing pattern for various skin diseases and to identify, monitor and assess any drug interactions and adverse reactions if any associated with their use. A prospective cross-sectional study on corticosteroid utilization pattern in dermatology department of a tertiary care teaching hospital was done by reviewing 205 inpatient cases for a period of 6 months. The data was collected with respect to demographics, medical and medication history, laboratory data and drugs prescribed during hospitalization and at discharge. A descriptive statistical analysis was performed to report the findings in terms of number and percentage. Corticosteroids were prescribed in 129 cases with 72male patients most in 51-60-year age group and 57female patients with 28% in 31-40-year age group. The common indications were psoriasis, dermatitis, and pemphigus. Prednisolone (27.4%) followed by clobetasol (22.04%) were most commonly prescribed. 48.6% therapy was by topical route, 28.2% by oral and 23.03% parenteral route. At discharge prednisolone and clobetasol were mostly prescribed and topical route was the most preferred route. Drug interactions involving drugs like betamethasone, triamcinolone, dexamethasone, and prednisolone were observed but no adverse reactions were reported. With a wide choice of corticosteroids and dosage forms available to treat dermatological conditions the rational therapy should consider the appropriate choice of drugs, potency, effectiveness and the cost.","PeriodicalId":16728,"journal":{"name":"Journal of Pharmaceutical Negative Results","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48458946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}