Polymorphism Of Virulence Genes Of Enterococcus Faecalis Associated With Aerobic Vaginitis

The aim of the current study is to determine the virulence genes of E. faecalis and polymorphism in these bacteria. A total of 300 females  who visited the outpatient clinics in the Maternity and Pediatrics Teaching Hospital in Al-Diwaniya city, Iraq were enrolled. For twenty isolates of E. faecalis that showed dense growth when cultured on the specified culture media, then the sensitivity to antibiotics was tested, and then the virulence genes were determined for each isolate, where the results were shown there are statistically significant differences (P<0.05) in the presence of virulence genes in the tested bacterial isolates. (where the genetic analysis by PCR showed the presence of all virulence genes, except for isolate 3 of the gene esp (Enterococcal surface production protein), and isolates 11, 12, 15, 16 and 19 of the hly gene (haemolysin production). ERlC results showed the most of the isolates share a DNA segment of length 1500, 1000 bp, and 2000 pb. The curve also shows that most of the isolates lack the presence of the segment of length 500 pb. As for the pieces of 700, 600 and 400 bp, they showed a very high heterogeneity between the isolates. ln conclusion, most genes responsible for virulence factors were positive, except for hly, which showed positive results for nearly more than half of the isolates, that the virulence factors of said bacteria are a major focus of infection in said bacteria, and if these genes are not available, they are unable to hit or stick to the surface of the vagina. There were two types of genetic polymorphism in strains, one of which is caused by horizontal transmission between strains via plasmids and other horizontal transmission methods, and the other is caused by vertical transmission through mutations that occur between generations, causing high resistance to some antibiotics. vitality.