Ryosuke Nogami, Mari Nagata, Risa Imada, Kenji Kai, Takashi Kawaguchi, Shuji Tani
{"title":"Cycloheximide in the nanomolar range inhibits seed germination of Orobanche minor","authors":"Ryosuke Nogami, Mari Nagata, Risa Imada, Kenji Kai, Takashi Kawaguchi, Shuji Tani","doi":"10.1584/jpestics.d23-038","DOIUrl":"https://doi.org/10.1584/jpestics.d23-038","url":null,"abstract":"</p><p>From the 992 samples of culture extracts of microorganisms isolated from soil in Japan, we found that the extract of <i>Streptomyces</i> sp. no. 226 inhibited <i>Orobanche minor</i> seed germination without significantly affecting the seed germination of <i>Trifolium pratense</i> and the growth of <i>Aspergillus oryzae</i> and <i>Escherichia coli</i>. Using ESI-MS, <sup>1</sup>H-NMR, and <sup>13</sup>C-NMR, we identified the active compound as cycloheximide. Cycloheximide had half-maximum inhibitory concentrations of 2.6 ng/mL for the inhibition of seed germination of <i>O. minor</i> and 2.5 µg/mL for that of the conidial germination of <i>A</i>. <i>oryzae</i>. Since cycloheximide is known to inhibit translation by interacting with ribosomal protein L28 (RPL28) in yeast, we investigated whether RPL protein of <i>O. minor</i> plays a critical role in the inhibition of <i>O. minor</i> seed germination. Our data suggested that <i>O. minor</i> RPL27A was not sensitive to cycloheximide by comparing it to the strain expressing <i>S. cerevisiae</i> RPL28. These findings suggest the presence of an unidentified mechanism by which cycloheximide hinders <i>O. minor</i> seed germination.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D23-038/figure/advpub_D23-038.png\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"24 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139055872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quantification of pendimethalin residue in green fodder and silage of winter cereals using gas chromatography-tandem mass spectrometry","authors":"Navjot Singh Brar, Kousik Mandal, Simerjeet Kaur, Amanpal Kaur Sandhu, Makhan Singh Bhullar, Maninder Pal Singh","doi":"10.1584/jpestics.d23-045","DOIUrl":"https://doi.org/10.1584/jpestics.d23-045","url":null,"abstract":"</p><p>Use of agro-chemicals in forage crops is restricted due to the fear of direct toxicity to livestock and risk of pesticide residue accumulation in the food chain. Wheat and barley can be used as green fodder and silage, and herbicide residue estimation in green fodder and silage is important for ensuring the safety of dairy cattle. A field experiment was conducted for two years to study pendimethalin residues in the green fodder and silage of wheat and barley. In both cereal crops, pendimethalin (1.125 kg a.i./ha) was applied as pre-emergence along with an unsprayed control. Pendimethalin residues in fodder, silage, and soil were estimated using gas chromatography-tandem mass spectrometry (GC-MS/MS). At harvest, pendimethalin residues in fodder and silage of wheat and barley were below the limit of quantification (<0.01 mg/kg) during both crop seasons. Pendimethalin can be safely used for weed control in winter cereals grown for fodder and silage.</p>\u0000<p></p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"163 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138502937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Author Index to Volume 48, 2023","authors":"","doi":"10.1584/jpestics.a23-03","DOIUrl":"https://doi.org/10.1584/jpestics.a23-03","url":null,"abstract":"","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"60 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139256321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Keyword Index to Volume 48, 2023","authors":"","doi":"10.1584/jpestics.a23-02","DOIUrl":"https://doi.org/10.1584/jpestics.a23-02","url":null,"abstract":"","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"8 10","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139259086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Contents of Volume 48, 2023","authors":"","doi":"10.1584/jpestics.a23-01","DOIUrl":"https://doi.org/10.1584/jpestics.a23-01","url":null,"abstract":"","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"14 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139255717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xin Wang, Yongqiang Zhang, Haofu Hu, Bin Liu, Feizhao Wang, Yang Zhang, Weiguo Wang, Xiaomeng Li, Wenping Xu
{"title":"Evaluation of the droplet deposition and control effect of a special adjuvant for unmanned aerial vehicle (UAV) sprayers.","authors":"Xin Wang, Yongqiang Zhang, Haofu Hu, Bin Liu, Feizhao Wang, Yang Zhang, Weiguo Wang, Xiaomeng Li, Wenping Xu","doi":"10.1584/jpestics.D22-048","DOIUrl":"https://doi.org/10.1584/jpestics.D22-048","url":null,"abstract":"<p><p>Unmanned aerial vehicle (UAV) sprayers have been widely used in agriculture. With the goals of using pesticides efficiently and reducing their dosage, we evaluated the effects of adding and not adding special adjuvants to UAV sprayers on droplet deposition and the control effect of leaf folder insects. The deposition quantity and coverage area of UAV sprayers with the Kao Adjuvant A-200® on rice leaves were better than those without the Kao Adjuvant A-200®. Regarding the control effect on rice leaf rollers, UAV sprayers with the Kao Adjuvant A-200® were also better, and they also met the pesticide residue limit for brown rice. Kao Adjuvant A-200® can improve the UAV sprayer's droplet deposition and pest control effect. When the pesticide dosage was reduced by 30%, UAV sprayers with Kao Adjuvant A-200® can achieve a good control effect, which is very helpful in reducing the pesticide dosage.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"48 3","pages":"78-85"},"PeriodicalIF":2.4,"publicationDate":"2023-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/86/81/jps-48-3-D22-048.PMC10513950.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41122793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Major latex-like proteins show pH dependency in their binding to hydrophobic organic pollutants.","authors":"Kentaro Fujita, Chihiro Sonoda, Maho Chujo, Hideyuki Inui","doi":"10.1584/jpestics.D23-014","DOIUrl":"https://doi.org/10.1584/jpestics.D23-014","url":null,"abstract":"<p><p>The Cucurbitaceae family accumulates hydrophobic organic pollutants in its aerial parts at high concentrations. Major latex-like proteins (MLPs) were identified in zucchini (<i>Cucurbita pepo</i>) as a transporting factor for hydrophobic organic pollutants. MLPs bind to hydrophobic organic pollutants in the roots, are secreted to xylem vessels as complexes, and are transported to the aerial parts. However, the suitable conditions for binding MLPs to hydrophobic organic pollutants remain elusive. In the present study, we show that MLPs bind to the hydrophobic organic pollutant pyrene with higher affinity under acidic conditions. Our results demonstrated that pH regulates the binding of MLPs to hydrophobic organic pollutants.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"48 3","pages":"71-77"},"PeriodicalIF":2.4,"publicationDate":"2023-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/a9/jps-48-3-D23-014.PMC10513956.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Insect growth-regulating activity of 1-benzyl-2-methylbenzimidazole derivatives on silkworms.","authors":"Shuhei Henmi, Ayumu Sueyoshi, Hiroshi Ono, Toshimasa Yamazaki, Konatsu Inoue, Takahiro Shiotsuki","doi":"10.1584/jpestics.D23-010","DOIUrl":"10.1584/jpestics.D23-010","url":null,"abstract":"<p><p>Derivatives of 1-benzyl-2-methylbenzimidazoles (BMBIs) were synthesized to evaluate their biological activities against <i>Bombyx mori</i>, a lepidopteran model insect. Synthesized BMBIs exhibited two different biological activities: inhibition of development and acute lethality. From a structural perspective, the activity varied with the position of the substitutions on the 1-benzyl moiety; BMBIs with substitutions on the 2 and/or 4 positions had comparatively high activity in comparison with those with substitutions on the 3-position. There was more activity for the inhibition of development with low doses, and more for acute lethality with high doses. The activity was also affected by the applied stage, that is, application in the 4th instar mostly interfered the larval molting or pupation, whereas that in the 3rd instar caused more acute mortality. Taken together, these results suggest that BMBIs have multiple modes of action.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"48 3","pages":"99-106"},"PeriodicalIF":1.5,"publicationDate":"2023-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/47/b8/jps-48-3-D23-010.PMC10513952.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of further substitutions at 5-, 6-, 7-, or 8-position of 3-[3-(4-methoxyphenyl)-1-hydroxyprop-2-yl]coumarin on phytotoxicity.","authors":"Satoshi Yamauchi, Hazna Sartiva, Hisashi Nishiwaki","doi":"10.1584/jpestics.D23-016","DOIUrl":"https://doi.org/10.1584/jpestics.D23-016","url":null,"abstract":"<p><p>Derivatives of the coumarin ring in (<i>R</i>)-3-[3-(4-methoxyphenyl)-1-hydroxyprop-2-yl]coumarin <b>2</b>, which is a lignan structure, were synthesized to clarify their structure-phytotoxicity relationships. The growth-inhibitory activity of the 8-OCH<sub>3</sub> derivative <b>8</b> (IC<sub>50</sub>=228 µM) was more potent against the roots of lettuce seedlings than the compound without substituents <b>2</b>. As for the roots of Italian ryegrass seedlings, the presence of the methoxy group at the 7- or 8-position was extremely effective for inhibiting growth (7-OCH<sub>3</sub> <b>7</b>: IC<sub>50</sub>=121 µM, 8-OCH<sub>3</sub> <b>8</b>: 56.7 µM). Methyl derivatives at the 5- or 8-position showed activity levels similar to those of the compound without substituents <b>2</b> (5-CH<sub>3</sub> <b>13</b>: IC<sub>50</sub>=214 µM, 8-CH<sub>3</sub> <b>16</b>: IC<sub>50</sub>=225 µM). The activities of OH- and F-derivatives were not observed or were lower.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"48 3","pages":"93-98"},"PeriodicalIF":2.4,"publicationDate":"2023-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f2/8e/jps-48-3-D23-016.PMC10513945.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41176497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fenoxycarb, a carbamate insect growth regulator, inhibits brassinosteroid action.","authors":"Shinsaku Ito, Kojiro Kawada, Yasumasa Saeki, Takeshi Nakano, Yasuyuki Sasaki, Shunsuke Yajima","doi":"10.1584/jpestics.D23-015","DOIUrl":"https://doi.org/10.1584/jpestics.D23-015","url":null,"abstract":"<p><p>Brassinosteroids (BRs) are steroid hormones that regulate plant growth, development, and stress resistance. In this study, we evaluated the effect of agrochemicals on dark-induced hypocotyl elongation, which is regulated by BRs, to identify novel chemicals that regulate BR action. We found that the juvenile hormone agonist fenoxycarb inhibited dark-induced hypocotyl elongation in <i>Arabidopsis</i>. Treatment with the same class of juvenile hormone agonist, pyriproxyfen, did not affect hypocotyl elongation. Co-treatment with fenoxycarb and BR partly canceled the fenoxycarb-induced hypocotyl suppression. In addition, gene expression analysis revealed that fenoxycarb altered the BR-responsive gene expression. These results indicate that fenoxycarb is a BR action inhibitor.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":"48 3","pages":"107-110"},"PeriodicalIF":2.4,"publicationDate":"2023-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/34/e4/jps-48-3-D23-015.PMC10513946.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41176512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}