Cycloheximide in the nanomolar range inhibits seed germination of Orobanche minor

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY
Ryosuke Nogami, Mari Nagata, Risa Imada, Kenji Kai, Takashi Kawaguchi, Shuji Tani
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Abstract

From the 992 samples of culture extracts of microorganisms isolated from soil in Japan, we found that the extract of Streptomyces sp. no. 226 inhibited Orobanche minor seed germination without significantly affecting the seed germination of Trifolium pratense and the growth of Aspergillus oryzae and Escherichia coli. Using ESI-MS, 1H-NMR, and 13C-NMR, we identified the active compound as cycloheximide. Cycloheximide had half-maximum inhibitory concentrations of 2.6 ng/mL for the inhibition of seed germination of O. minor and 2.5 µg/mL for that of the conidial germination of A. oryzae. Since cycloheximide is known to inhibit translation by interacting with ribosomal protein L28 (RPL28) in yeast, we investigated whether RPL protein of O. minor plays a critical role in the inhibition of O. minor seed germination. Our data suggested that O. minor RPL27A was not sensitive to cycloheximide by comparing it to the strain expressing S. cerevisiae RPL28. These findings suggest the presence of an unidentified mechanism by which cycloheximide hinders O. minor seed germination.

Abstract Image Fullsize Image
纳摩尔范围内的环己亚胺可抑制小鹅掌楸种子的萌发
在从日本土壤中分离的 992 份微生物培养物提取物样本中,我们发现链霉菌 226 号提取物可抑制小鹅掌楸种子萌发,但对三叶草种子萌发以及米曲霉和大肠杆菌的生长无明显影响。利用 ESI-MS、1H-NMR 和 13C-NMR 技术,我们确定了活性化合物为环己亚胺。环己亚胺对小杆线虫种子萌发的半最大抑制浓度为 2.6 纳克/毫升,对大肠杆菌分生孢子萌发的半最大抑制浓度为 2.5 微克/毫升。众所周知,环己亚胺能通过与酵母中的核糖体蛋白 L28(RPL28)相互作用来抑制翻译,因此我们研究了欧鼠李的 RPL 蛋白在抑制欧鼠李种子萌发中是否起着关键作用。我们的数据表明,通过与表达 S. cerevisiae RPL28 的菌株比较,小尾寒羊 RPL27A 对环己亚胺不敏感。这些研究结果表明,环己亚胺阻碍小麦角种子萌发的机制尚未确定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
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