Journal of Neuropathology and Experimental Neurology最新文献_第6页

Persistent increase of Nogo-A-positive cells and dynamic reduction in oligodendroglia lineage cells in white matter regions following experimental and clinical traumatic brain injury. 实验性和临床创伤性脑损伤后，白质区nogo - a阳性细胞持续增加，少突胶质细胞谱系动态减少。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-05-01 DOI: 10.1093/jnen/nlaf017

Karsten Ruscher, Georgios Michalettos, Sami Abu Hamdeh, Fredrik Clausen, Amber L Nolan, Johanna Flygt, Ilknur Özen, Niklas Marklund

{"title":"Persistent increase of Nogo-A-positive cells and dynamic reduction in oligodendroglia lineage cells in white matter regions following experimental and clinical traumatic brain injury.","authors":"Karsten Ruscher, Georgios Michalettos, Sami Abu Hamdeh, Fredrik Clausen, Amber L Nolan, Johanna Flygt, Ilknur Özen, Niklas Marklund","doi":"10.1093/jnen/nlaf017","DOIUrl":"10.1093/jnen/nlaf017","url":null,"abstract":"White matter (WM) disruption and atrophy is a consequence of traumatic brain injury (TBI) and contributes to persisting cognitive impairment. An increased expression of the myelin-associated axonal outgrowth inhibitor Nogo-A and oligodendrocyte pathology might be negatively associated with postinjury WM changes. Here, we analyzed brain tissue from severe TBI patients, obtained by surgical decompression in the early postinjury phase and postmortem brain tissue of long-term TBI survivors and observed an increased number of Nogo-A+ cells in WM tracts such as the corpus callosum (CC). Likewise, the number of Nogo-A+ cells in the CC was increased from day 7 postinjury to 6 months postinjury (mpi) following central fluid percussion injury (cFPI) in mice. In addition, the number of Olig2+ cells in the CC and capsula externa remained constant, while the numbers of Olig2+/CC1+ and GST-π+ mature oligodendrocytes declined throughout the observation time of 18 months. A significantly lower number of Olig2+/CC1+ cells was found in cFPI mice compared to controls at 18 mpi. Persistent vulnerability of oligodendrocytes in combination with dynamic alterations of Nogo-A expression may have implications for the WM atrophy and insufficient recovery observed after TBI.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":"423-435"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concurrent Pit-1 and SALL4 staining in primitive component of pituitary blastoma: A case report with clinicopathologic features including Cushing syndrome presentation, germline DICER1 mutation, and significant therapeutic response to chemoradiation. 垂体胚泡瘤原始成分中同时出现的 Pit-1 和 SALL4 染色：一例临床病理特征包括库欣综合征表现、种系 DICER1 基因突变和对化疗的显著治疗反应的病例报告。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-05-01 DOI: 10.1093/jnen/nlae126

Julieann C Lee, Amar Gajjar, Melissa R Perrino, Angela Delaney, Christopher L Tinkle, Paul Klimo, Larissa V Furtado, Yen-Chun Liu, Alex Breuer, Soniya N Pinto, Asim K Bag, David W Ellison, Brent A Orr

引用次数: 0

Maximum contrast projection: A powerful tool for biomedical image stack analysis. 最大对比度投影：生物医学图像堆栈分析的强大工具。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-05-01 DOI: 10.1093/jnen/nlaf013

Nat Adamian, Christopher Guirguis, Ansel Link, Nate Jowett, Iván Coto Hernández

{"title":"Maximum contrast projection: A powerful tool for biomedical image stack analysis.","authors":"Nat Adamian, Christopher Guirguis, Ansel Link, Nate Jowett, Iván Coto Hernández","doi":"10.1093/jnen/nlaf013","DOIUrl":"10.1093/jnen/nlaf013","url":null,"abstract":"Maximum intensity projection is a simple post-hoc dimension reduction approach that yields sharp 2-dimensional images from image stacks but is limited by low signal or high background. Herein, we demonstrated improvement in image contrast using maximum contrast versus maximum intensity projection in 3-dimensional phase-contrast and quantitative phase imaging of frozen cross-sections of murine peripheral nerve. Fresh frozen murine sciatic nerve sections were imaged using 3-dimensional fluorescence, phase-contrast, and quantitative phase microscopy. Images were processed using maximum intensity and maximum contrast projection. Higher image contrast of nerve morphology was obtained when the images were processed with maximum contrast projection. Enhanced image contrast in quantitative phase imaging facilitated label-free multicolor imaging of murine peripheral nerves. Herein, we demonstrate the utility of maximum contrast projection in enhancing image contrast over intensity projection approaches in fluorescence phase-contrast and quantitative phase imaging of nerve histological samples. A user-friendly, open-source Python-based maximum contrast projection algorithm to facilitate the adoption of this technique is provided.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":"391-397"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different cortical and subcortical astroglial responsiveness in rats with acute liver failure. 急性肝衰竭大鼠皮层和皮层下星形胶质细胞反应性的差异。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-05-01 DOI: 10.1093/jnen/nlaf020

Nahla Ouard, Assmaâ Tali, Themoi Demsou Souhoudji, Rajâa Jebbouj, Ihssane El-Bouchikhi, Christopher F Rose, Samir Ahboucha

{"title":"Different cortical and subcortical astroglial responsiveness in rats with acute liver failure.","authors":"Nahla Ouard, Assmaâ Tali, Themoi Demsou Souhoudji, Rajâa Jebbouj, Ihssane El-Bouchikhi, Christopher F Rose, Samir Ahboucha","doi":"10.1093/jnen/nlaf020","DOIUrl":"10.1093/jnen/nlaf020","url":null,"abstract":"Hepatic encephalopathy (HE) is a neuropsychiatric complication of liver failure. Previous studies described astroglia alterations in HE, but regional changes have not been well investigated. This study addresses regional astroglial response by exploring glial fibrillary acidic protein (GFAP) immunoreactivity in cortical structures including somatosensory (S1Tr and S1BF), piriform (Pir), and perirhinal (PRh) cortices, and subcortical regions including corpus callosum (CC), ventromedial thalamus (VMT), mammillothalamic tract (MTT), and dorsomedial hypothalamic nucleus (DHN) in rats with acute liver failure (ALF) sacrificed at coma stage. Our data showed decreased numbers of astrocytes in S1Tr, Pir, and CC in ALF rats. GFAP-immunoreactive cells were increased within other regions including PRh, VMT, MTT, and DHN. Cell morphometric analysis showed significant increase in GFAP-immunoreactive astrocyte processes and cell bodies in cortical and subcortical regions but not in CC and DHN. However, astrocyte perimeters were increased, particularly in S1Tr and VMT. Our study demonstrates regional specificity including (1) regions with astrocyte activation associated with an increase of GFAP-immunostaining and astrocyte cell counts, together with (2) unaltered GFAP components, and (3) regions characterized by presumably inactive astrocyte with a reduced GFAP-immunostaining. These findings may reflect either different regional alterations in HE, or stages of an alteration progressing differently in different regions.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":"412-422"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Authors' response to Dr Miller's letter: "Machaalani et al. concerning dentate gyrus dysplasia". 作者对米勒博士来信的回应：“Machaalani等人关于齿状回发育不良”。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-05-01 DOI: 10.1093/jnen/nlaf012

Rita Machaalani, Michael Rodriguez

引用次数: 0

Presenilin 1 M139I mutation regulates the microRNA-34a-mediated neurogenic locus notch homolog protein 1 signaling pathway in an early-onset Alzheimer disease cell model. 早老素1m139i突变调控早发性阿尔茨海默病细胞模型中microrna -34a介导的神经源性基因座缺口同源蛋白1信号通路

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-26 DOI: 10.1093/jnen/nlaf044

Xuechun Sun, Lijun Dai, Xin Yuan, Lufeng Cheng, Jing Wang, Ye Tian, Lingyan Zhou

{"title":"Presenilin 1 M139I mutation regulates the microRNA-34a-mediated neurogenic locus notch homolog protein 1 signaling pathway in an early-onset Alzheimer disease cell model.","authors":"Xuechun Sun, Lijun Dai, Xin Yuan, Lufeng Cheng, Jing Wang, Ye Tian, Lingyan Zhou","doi":"10.1093/jnen/nlaf044","DOIUrl":"https://doi.org/10.1093/jnen/nlaf044","url":null,"abstract":"Presenilin 1 (PSEN1) mutations are the leading cause of early-onset Alzheimer disease (EOAD). A recent study found that the PSEN1 M139I mutation is associated with EOAD. In this study, we examined the impact of the PSEN1 M139I mutation in an EOAD in vitro model. Our findings reveal that the PSEN1 M139I mutation leads to increased levels of Aβ42/40, Hairy and Enhancer of Split-1 (Hes1), neurogenic locus notch homolog intracellular domain, and microRNA-34a, accompanied by a decrease in the level of neurogenic locus notch homolog protein 1 (NOTCH-1). Computational predictions indicate that NOTCH-1 is a direct target of microRNA-34a. Transfection of microRNA-34a mimics into PSEN1 M139I mutant SH-SY5Y cells increased the ratio of Aβ42/40 and induced Hes1, cysteine-aspartic acid protease 3 (Caspase-3), and apoptosis while reducing the NOTCH-1 expression and inhibiting cell proliferation. Conversely, downregulating microRNA-34a expression by transfecting microRNA-34a inhibitors mitigated these effects, thereby restoring NOTCH-1 production and cell proliferation and reversing the increases in Aβ42/40 ratio, Hes1, Caspase-3, and apoptosis induced by the PSEN1 M139I mutation. In summary, the PSEN1 M139I mutation identified in EOAD may influence amyloid-β (Aβ) production and apoptosis by regulating the microRNA-34a-mediated NOTCH-1 signaling pathway.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EGFR transcript variants: Potential diagnostic biomarker for glioblastoma, IDH-wildtype? EGFR转录变体：胶质母细胞瘤的潜在诊断生物标志物，idh野生型？

IF 3 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-25 DOI: 10.1093/jnen/nlaf041

David J Cook, Paul A Decker, Jayson J Hardcastle, Tom M Kollmeyer, Stephanie A Smoley, Matthew D Isaacson, Mallika Gandham, Surendra Dasari, Zied Abdullaev, Kenneth Aldape, Martha Quezado, Patrick J Cimino, Drew W Pratt, Caterina Giannini, Aivi Nguyen, Aditya Raghunathan, Jorge A Trejo-Lopez, Rachael A Vaubel, M Adelita Vizcaino, Cinthya J Zepeda Mendoza, Hussam Al Kateb, Robert B Jenkins, Cristiane M Ida

引用次数: 0

Adequate capture of spatial heterogeneity of Ki-67 proliferative index in meningiomas requires multiple tissue sections. 脑膜瘤中Ki-67增殖指数的空间异质性需要多次组织切片。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-21 DOI: 10.1093/jnen/nlaf043

Ugochukwu Ugwuowo, Bethany Faust, Haiming Tang, Marcello DiStasio

引用次数: 0

Characterization of Chitinase 3-like protein 1 spatiotemporal distribution in human post-traumatic brain contusions and other neuropathological scenarios. 几丁质酶3样蛋白1在人创伤后脑挫伤及其他神经病理情景中的时空分布特征

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-01 DOI: 10.1093/jnen/nlaf002

Cristina Sánchez Carabias, Victoria Cunha Alves, Aurelio Hernández Laín, Alfonso Lagares

{"title":"Characterization of Chitinase 3-like protein 1 spatiotemporal distribution in human post-traumatic brain contusions and other neuropathological scenarios.","authors":"Cristina Sánchez Carabias, Victoria Cunha Alves, Aurelio Hernández Laín, Alfonso Lagares","doi":"10.1093/jnen/nlaf002","DOIUrl":"10.1093/jnen/nlaf002","url":null,"abstract":"Chitinase 3-like protein 1 (CHI3L1) is emerging as a promising biomarker for assessing intracranial lesion burden and predicting prognosis in traumatic brain injury (TBI) patients. Following experimental TBI, Chi3l1 transcripts were detected in reactive astrocytes located within the pericontusional cortex. However, the cellular sources of CHI3L1 in response to hemorrhagic contusions in human brain remain unidentified. Hence, we examined a comprehensive collection of histologically defined acute and subacute human cerebral contusions with various surgical intervals using immunohistochemistry, validated through double immunofluorescence for markers such as GFAP, NeuN, MBP, and Iba-1, along with Fluoro-Jade C histofluorescence staining. CHI3L1 was found at meningeal interfaces, showing significant thickening of subpial glial plate. Paradoxically, CHI3L1-positive astrocytes were identified in neuroanatomical locations distant from hemorrhagic foci, where numerous eosinophilic ischemic neurons also exhibited CHI3L1 immunoreactivity. CHI3L1 immunostaining extended into white matter tracts and highlighted various phagocytic or activated microglia forms after delayed surgical decompressions. Given these findings, we advise against using CHI3L1 as a reactive astrogliosis marker due to its expression in multiple cell types, including astrocytes, neurons, oligodendrocytes, ependymocytes, leptomeningeal cells, microglia, and blood vessels. This non-selective response underscores the potential for CHI3L1 elevation patterns in biofluids to reflect the overall lesion burden extent.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":"305-328"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intramural hematomas and astrocytic infiltration precede perivascular inflammation in a rat model of repetitive low-level blast injury. 在大鼠重复性低水平爆炸损伤模型中，壁内血肿和星形细胞浸润先于血管周围炎症。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-01 DOI: 10.1093/jnen/nlaf003

Miguel A Gama Sosa, Rita De Gasperi, Rachel H Lind, Dylan Pryor, Danielle C Vargas, Georgina S Perez Garcia, Gissel M Perez, Rania Abutarboush, Usmah Kawoos, Allison Sowa, Carolyn W Zhu, William G M Janssen, Patrick R Hof, Stephen T Ahlers, Gregory A Elder

{"title":"Intramural hematomas and astrocytic infiltration precede perivascular inflammation in a rat model of repetitive low-level blast injury.","authors":"Miguel A Gama Sosa, Rita De Gasperi, Rachel H Lind, Dylan Pryor, Danielle C Vargas, Georgina S Perez Garcia, Gissel M Perez, Rania Abutarboush, Usmah Kawoos, Allison Sowa, Carolyn W Zhu, William G M Janssen, Patrick R Hof, Stephen T Ahlers, Gregory A Elder","doi":"10.1093/jnen/nlaf003","DOIUrl":"10.1093/jnen/nlaf003","url":null,"abstract":"In modern war theaters, exposures to blast overpressures are one of the most common causes of brain injury. These pervasive events result in acute and chronic cerebrovascular degenerative processes. Using a rat model of blast-induced mild traumatic brain injury, we identified intramural periarterial hematomas as early primary acute lesions induced by blast exposures. These lesions resulted in intravascular cell death, cell layer reorganization, and plasma leakage into the intraperiarterial basal membranes that constitute the intraperiarterial drainage system (IPAD). Plasma metalloproteases, including MMP-9, in the IPAD basal membranes may degrade extracellular matrix components compromising normal cerebral interstitial fluid drainage, arterial structure and function leading to chronic vascular degenerative processes. Related subacute effects of blast exposure included increased MMP-9 expression in perivascular reactive astrocytes and the extension of astrocytic processes through the layers of affected vessels. These results, in combination with normal levels of proinflammatory cytokines and the absence of proinflammatory MHC II-expressing microglia, suggest an astrocytic role in the clearing of intravascular hematomas and provide further mechanistic evidence that blast-induced vascular degenerative processes may precede the onset of neurovascular inflammation.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":"337-352"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0