Journal of Neuropathology and Experimental Neurology最新文献

Persistent increase of Nogo-A-positive cells and dynamic reduction in oligodendroglia lineage cells in white matter regions following experimental and clinical traumatic brain injury.

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-03-14 DOI: 10.1093/jnen/nlaf017

Karsten Ruscher, Georgios Michalettos, Sami Abu Hamdeh, Fredrik Clausen, Amber L Nolan, Johanna Flygt, Ilknur Özen, Niklas Marklund

{"title":"Persistent increase of Nogo-A-positive cells and dynamic reduction in oligodendroglia lineage cells in white matter regions following experimental and clinical traumatic brain injury.","authors":"Karsten Ruscher, Georgios Michalettos, Sami Abu Hamdeh, Fredrik Clausen, Amber L Nolan, Johanna Flygt, Ilknur Özen, Niklas Marklund","doi":"10.1093/jnen/nlaf017","DOIUrl":"https://doi.org/10.1093/jnen/nlaf017","url":null,"abstract":"White matter (WM) disruption and atrophy is a consequence of traumatic brain injury (TBI) and contributes to persisting cognitive impairment. An increased expression of the myelin-associated axonal outgrowth inhibitor Nogo-A and oligodendrocyte pathology might be negatively associated with postinjury WM changes. Here, we analyzed brain tissue from severe TBI patients, obtained by surgical decompression in the early postinjury phase and postmortem brain tissue of long-term TBI survivors and observed an increased number of Nogo-A+ cells in WM tracts such as the corpus callosum (CC). Likewise, the number of Nogo-A+ cells in the CC was increased from day 7 postinjury to 6 months postinjury (mpi) following central fluid percussion injury (cFPI) in mice. In addition, the number of Olig2+ cells in the CC and capsula externa remained constant, while the numbers of Olig2+/CC1+ and GST-π+ mature oligodendrocytes declined throughout the observation time of 18 months. A significantly lower number of Olig2+/CC1+ cells was found in cFPI mice compared to controls at 18 mpi. Persistent vulnerability of oligodendrocytes in combination with dynamic alterations of Nogo-A expression may have implications for the WM atrophy and insufficient recovery observed after TBI.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Authors' response to Dr Miller's letter: "Machaalani et al. concerning dentate gyrus dysplasia".

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-03-14 DOI: 10.1093/jnen/nlaf012

Rita Machaalani, Michael Rodriguez

引用次数: 0

Correlating hippocampal and amygdala volumes with neuropathological burden in Down syndrome and Alzheimer's disease and related neurodegenerative pathologies using 7T postmortem MRI.

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-03-10 DOI: 10.1093/jnen/nlaf010

Jr-Jiun Liou, Jinghang Li, Jacob Berardinelli, Hecheng Jin, Tales Santini, Jaehoon Noh, Nadim Farhat, Minjie Wu, Howard J Aizenstein, Joseph M Mettenburg, William H Yong, Elizabeth Head, Milos D Ikonomovic, Tamer S Ibrahim, Julia K Kofler

{"title":"Correlating hippocampal and amygdala volumes with neuropathological burden in Down syndrome and Alzheimer's disease and related neurodegenerative pathologies using 7T postmortem MRI.","authors":"Jr-Jiun Liou, Jinghang Li, Jacob Berardinelli, Hecheng Jin, Tales Santini, Jaehoon Noh, Nadim Farhat, Minjie Wu, Howard J Aizenstein, Joseph M Mettenburg, William H Yong, Elizabeth Head, Milos D Ikonomovic, Tamer S Ibrahim, Julia K Kofler","doi":"10.1093/jnen/nlaf010","DOIUrl":"https://doi.org/10.1093/jnen/nlaf010","url":null,"abstract":"Limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC), is common in elderly brains and often seen in conjunction with Alzheimer's disease neuropathologic change (ADNC). LATE-NC typically begins in the amygdala and spreads to the hippocampus and neocortex. Whether it contributes to hippocampal and amygdala atrophy in Down syndrome (DS) remains unexplored. We analyzed amygdala and hippocampal volumes and neuropathological burden in 12 DS cases and 54 non-DS cases with AD and related neurodegenerative pathologies (ADRNP) using 7 Tesla (7T) postmortem ex vivo MRI. Postmortem and antemortem hippocampal volumes were significantly correlated in a subset of 17 cases with available antemortem MRI scans. DS cases had smaller hippocampal and amygdala volumes than ADRNP cases; these correlated with more severe Braak stage but not with Thal phase. LATE-NC and hippocampal sclerosis (HS) were uncommon in DS cases. In ADRNP cases, lower hippocampal volumes associated with dementia duration, advanced Thal phase, Braak NFT stage, C score, LATE-NC stage, HS and arteriolosclerosis severity; reduced amygdala volumes correlated with severe LATE-NC stage, HS, and arteriolosclerosis severity, but not with Thal phase or Braak NFT stage. Lewy body pathology did not affect hippocampal or amygdala volume in either cohort. Thus, hippocampal volumes in ADRNP were influenced by both ADNC and LATE-NC, and amygdala volumes were primarily influenced by LATE-NC. In DS, hippocampal and amygdala volumes were primarily influenced by tau pathology.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cortical variant multiple sclerosis presenting clinically as Lewy body dementia.

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-03-06 DOI: 10.1093/jnen/nlaf015

Delaney Liskey, Alexia R Maier, Shunsuke Koga, Sarosh Irani, Neill Graff-Radford, Keith A Josephs, Dennis W Dickson, Shanu F Roemer

引用次数: 0

Insights in the underlying pathophysiology of brain malformations associated with VRK1-related syndrome derived from fetal neuropathology.

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-03-06 DOI: 10.1093/jnen/nlaf014

Aude Tessier, Olivier Monestier, François Guillaume Debray, Fréderic Vauthier, Valerie Benoit, Kim Van Berkel, Leila Ghassemi, Stefanie Brock

引用次数: 0

Re: Article by Machaalani et al. concerning dentate gyrus dysplasia.

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-03-02 DOI: 10.1093/jnen/nlaf011

Douglas C Miller

引用次数: 0

Maximum contrast projection: A powerful tool for biomedical image stack analysis.

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-03-02 DOI: 10.1093/jnen/nlaf013

Nat Adamian, Christopher Guirguis, Ansel Link, Nate Jowett, Iván Coto Hernández

{"title":"Maximum contrast projection: A powerful tool for biomedical image stack analysis.","authors":"Nat Adamian, Christopher Guirguis, Ansel Link, Nate Jowett, Iván Coto Hernández","doi":"10.1093/jnen/nlaf013","DOIUrl":"https://doi.org/10.1093/jnen/nlaf013","url":null,"abstract":"Maximum intensity projection is a simple post-hoc dimension reduction approach that yields sharp 2-dimensional images from image stacks but is limited by low signal or high background. Herein, we demonstrated improvement in image contrast using maximum contrast versus maximum intensity projection in 3-dimensional phase-contrast and quantitative phase imaging of frozen cross-sections of murine peripheral nerve. Fresh frozen murine sciatic nerve sections were imaged using 3-dimensional fluorescence, phase-contrast, and quantitative phase microscopy. Images were processed using maximum intensity and maximum contrast projection. Higher image contrast of nerve morphology was obtained when the images were processed with maximum contrast projection. Enhanced image contrast in quantitative phase imaging facilitated label-free multicolor imaging of murine peripheral nerves. Herein, we demonstrate the utility of maximum contrast projection in enhancing image contrast over intensity projection approaches in fluorescence phase-contrast and quantitative phase imaging of nerve histological samples. A user-friendly, open-source Python-based maximum contrast projection algorithm to facilitate the adoption of this technique is provided.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acquired intradiploic epidermoid cyst: A rare case report with literature review. 后天性腹膜内表皮样囊肿：罕见病例报告及文献综述

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-02-21 DOI: 10.1093/jnen/nlae106

Jacob A Schroeder, Mohammed Sabawi, Amr H Masaadeh, Kathryn L Eschbacher, Nitesh Shekhrajka, Márcio Luís Duarte, Leonardo Furtado Freitas

引用次数: 0

An analysis of RNA quality metrics in human brain tissue. 人脑组织中RNA质量指标的分析。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-02-21 DOI: 10.1093/jnen/nlae132

Jiahe Tian, Tiffany G Lam, Sophie K Ross, Benjamin Ciener, Sandra Leskinen, Sharanya Sivakumar, David A Bennett, Vilas Menon, Guy M McKhann, Alexi Runnels, Andrew F Teich

{"title":"An analysis of RNA quality metrics in human brain tissue.","authors":"Jiahe Tian, Tiffany G Lam, Sophie K Ross, Benjamin Ciener, Sandra Leskinen, Sharanya Sivakumar, David A Bennett, Vilas Menon, Guy M McKhann, Alexi Runnels, Andrew F Teich","doi":"10.1093/jnen/nlae132","DOIUrl":"10.1093/jnen/nlae132","url":null,"abstract":"Human brain tissue studies have used a range of metrics to assess RNA quality but there are few large-scale cross-comparisons of presequencing quality metrics with RNA-seq quality. We analyzed how postmortem interval (PMI) and RNA integrity number (RIN) before RNA-seq relate to RNA quality after sequencing (percent of counts in top 10 genes [PTT], 5' bias, and 3' bias), and with individual gene counts across the transcriptome. We analyzed 4 human cerebrocortical tissue sets (1 surgical, 3 autopsy), sequenced with varying protocols. Postmortem interval and RIN had a low inverse correlation (down to r = -0.258, P < .001 across the autopsy cohorts); both PMI and RIN showed consistent and opposing correlations with PTT (up to r = 0.215, P < .001 for PMI and down to r = -0.677, P < .001 for RIN across the autopsy cohorts). Unlike PMI, RIN showed consistent correlations with measurements of 3' and 5' bias in autopsies (r = -0.366, P < .001 with 3' bias). RNA integrity number correlated with 3933 genes across the 4 datasets vs 138 genes for PMI. Neuronal and immune response genes correlated positively and negatively with RIN, respectively. Thus, different gene sets have divergent relationships with RIN. These analyses suggest that conventional metrics of RNA quality have varying values and that PMI has an overall modest effect on RNA quality.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":"236-243"},"PeriodicalIF":3.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alzheimer disease-associated tau post-translational modification mimics impact tau propagation and uptake.

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-02-21 DOI: 10.1093/jnen/nlaf007

John R Dickson, Robert G R Sobolewski, Analiese R Fernandes, Joanna M Cooper, Zhanyun Fan, Mirra Chung, Cameron Donahue, Derek H Oakley, Dudley K Strickland, Bradley T Hyman

{"title":"Alzheimer disease-associated tau post-translational modification mimics impact tau propagation and uptake.","authors":"John R Dickson, Robert G R Sobolewski, Analiese R Fernandes, Joanna M Cooper, Zhanyun Fan, Mirra Chung, Cameron Donahue, Derek H Oakley, Dudley K Strickland, Bradley T Hyman","doi":"10.1093/jnen/nlaf007","DOIUrl":"10.1093/jnen/nlaf007","url":null,"abstract":"As Alzheimer disease (AD) progresses, pathological tau spreads by cell-to-cell propagation of tau. This study aims to elucidate the impact of AD-associated post-translational modifications of tau-on-tau propagation. Tau propagation reporter constructs distinguishing donor cells from recipient cells were developed, and additional constructs were made with tau residues mutated from serine or threonine to aspartate to mimic the negative charge of a phosphorylation and/or from lysine to glutamine to mimic the charge-neutralizing effect of acetylation. Flow cytometry was used to quantify donor and recipient cells. This revealed that the mutations generally tended to reduce tau propagation compared to wildtype tau. Recombinant tau containing either wildtype or posttranslational modification mimicking mutations were used to treat Chinese hamster ovary cells or human induced pluripotent stem cell-derived neurons to quantify tau uptake, revealing that the mutations generally resulted in reduced uptake compared to wildtype tau. Surface plasmon resonance revealed that the mutations had a reduced affinity for lipoprotein receptor-related protein 1 (LRP1), a tau uptake receptor, compared to wildtype tau. Overall, these results suggest that AD-associated posttranslational modification mimicking mutations reduce the cell-to-cell propagation of tau by reducing tau uptake by recipient cells, which may be in part due to reduced binding affinity to LRP1.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0