Persistent increase of Nogo-A-positive cells and dynamic reduction in oligodendroglia lineage cells in white matter regions following experimental and clinical traumatic brain injury.

IF 3.2 3区 医学 Q2 CLINICAL NEUROLOGY
Karsten Ruscher, Georgios Michalettos, Sami Abu Hamdeh, Fredrik Clausen, Amber L Nolan, Johanna Flygt, Ilknur Özen, Niklas Marklund
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Abstract

White matter (WM) disruption and atrophy is a consequence of traumatic brain injury (TBI) and contributes to persisting cognitive impairment. An increased expression of the myelin-associated axonal outgrowth inhibitor Nogo-A and oligodendrocyte pathology might be negatively associated with postinjury WM changes. Here, we analyzed brain tissue from severe TBI patients, obtained by surgical decompression in the early postinjury phase and postmortem brain tissue of long-term TBI survivors and observed an increased number of Nogo-A+ cells in WM tracts such as the corpus callosum (CC). Likewise, the number of Nogo-A+ cells in the CC was increased from day 7 postinjury to 6 months postinjury (mpi) following central fluid percussion injury (cFPI) in mice. In addition, the number of Olig2+ cells in the CC and capsula externa remained constant, while the numbers of Olig2+/CC1+ and GST-π+ mature oligodendrocytes declined throughout the observation time of 18 months. A significantly lower number of Olig2+/CC1+ cells was found in cFPI mice compared to controls at 18 mpi. Persistent vulnerability of oligodendrocytes in combination with dynamic alterations of Nogo-A expression may have implications for the WM atrophy and insufficient recovery observed after TBI.

实验性和临床创伤性脑损伤后,白质区nogo - a阳性细胞持续增加,少突胶质细胞谱系动态减少。
白质(WM)破坏和萎缩是创伤性脑损伤(TBI)的后果,并有助于持续的认知障碍。髓鞘相关轴突生长抑制剂Nogo-A的表达增加和少突胶质细胞病理可能与损伤后WM变化负相关。在这里,我们分析了严重TBI患者的脑组织,分别在损伤后早期和长期TBI幸存者死后的脑组织中通过手术减压获得,并观察到胼胝体(CC)等WM束中Nogo-A+细胞数量增加。同样,小鼠中枢性液体冲击损伤(cFPI)后,从损伤后第7天到损伤后6个月(mpi), CC中Nogo-A+细胞数量增加。此外,在18个月的观察时间内,CC和外包膜中Olig2+细胞的数量保持不变,而Olig2+/CC1+和GST-π+成熟少突胶质细胞的数量下降。与对照组相比,在18 mpi时,cFPI小鼠的Olig2+/CC1+细胞数量明显减少。少突胶质细胞的持续脆弱性与Nogo-A表达的动态变化可能与脑外伤后WM萎缩和恢复不足有关。
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来源期刊
CiteScore
5.40
自引率
6.20%
发文量
118
审稿时长
6-12 weeks
期刊介绍: Journal of Neuropathology & Experimental Neurology is the official journal of the American Association of Neuropathologists, Inc. (AANP). The journal publishes peer-reviewed studies on neuropathology and experimental neuroscience, book reviews, letters, and Association news, covering a broad spectrum of fields in basic neuroscience with an emphasis on human neurological diseases. It is written by and for neuropathologists, neurologists, neurosurgeons, pathologists, psychiatrists, and basic neuroscientists from around the world. Publication has been continuous since 1942.
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