Journal of Neuropathology and Experimental Neurology最新文献_第5页

Evaluation of 6 monoclonal antibodies against Ser129-phosphorylated α-synuclein: Critical role of proteinase K antigen retrieval and superior sensitivity of the D1R1R clone in human skin biopsies. 6种抗ser129磷酸化α-突触核蛋白单克隆抗体的评价：蛋白酶K抗原检索的关键作用和D1R1R克隆在人皮肤活检中的优越敏感性

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-22 DOI: 10.1093/jnen/nlaf036

Cecilia Delprete, Alex Incensi, Alessandro Furia, Riccardo Bari, Rocco Liguori, Vincenzo Donadio

{"title":"Evaluation of 6 monoclonal antibodies against Ser129-phosphorylated α-synuclein: Critical role of proteinase K antigen retrieval and superior sensitivity of the D1R1R clone in human skin biopsies.","authors":"Cecilia Delprete, Alex Incensi, Alessandro Furia, Riccardo Bari, Rocco Liguori, Vincenzo Donadio","doi":"10.1093/jnen/nlaf036","DOIUrl":"https://doi.org/10.1093/jnen/nlaf036","url":null,"abstract":"α-Synuclein is an essential component of synucleinopathies including Parkinson disease, dementia with Lewy bodies, and multiple system atrophy (MSA). Misfolded-α-synuclein inclusions that contain high levels of Serine-129 phosphorylated (pS129-α-syn) are key diagnostic markers. Skin biopsies are a promising peripheral tissue for in vivo detection of aggregates using immunofluorescence staining. Several primary antibodies target pS129-α-syn but their diagnostic reliability remains uncertain. Common practice relies on clones EP1536Y and 81A without antigen retrieval; however, recent findings have underscored the need to validate additional methodologies and alternative clones. We compared the diagnostic accuracy of the standard protocol, alongside formic acid and proteinase K (PK) antigen retrieval to evaluate 4 additional monoclonal antibodies (J18, BBF19, pSyn#64, and D1R1R) in a cohort of 43 confirmed synucleinopathy patients (7 with MSA) and 33 healthy controls. The results showed that PK increased the detection rates for EP1536Y, 81A, and D1R1R, with D1R1R outperforming the others in sensitivity. J18, BBF19, and pSyn#64 exhibited insufficient specificity, limiting their clinical applicability. The improved accuracy with PK treatment and the promising performance of D1R1R mark critical advancements for reliable diagnosis, highlighting the importance of optimizing protocols and validating antibodies for dependable detection of pathological aggregates in skin biopsies.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adequate capture of spatial heterogeneity of Ki-67 proliferative index in meningiomas requires multiple tissue sections. 脑膜瘤中Ki-67增殖指数的空间异质性需要多次组织切片。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-21 DOI: 10.1093/jnen/nlaf043

Ugochukwu Ugwuowo, Bethany Faust, Haiming Tang, Marcello DiStasio

引用次数: 0

Letter to the Editor: Molecularly defined oligodendroglioma evolving to osseous metastases. 致编辑的信：分子定义的少突胶质细胞瘤进化为骨转移瘤。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-18 DOI: 10.1093/jnen/nlaf038

Emily Ling-Lin Pai, Yuji Matsumoto, Dan Dou, Shweta Nelluri, Pooja Devi, Salvatore Priori, Nduka Amankulor, Michelle Alonso-Basanta, Omkar Singh, Zied Abdullaev, Kenneth Aldape, Arati Desai, Suyash Mohan, MacLean P Nasrallah

引用次数: 0

Correction to: Comorbidities in early-onset sporadic versus presenilin-1 mutation-associated Alzheimer disease dementia: Evidence for dependency on Alzheimer disease neuropathological changes. 纠正：早发散发性与早老素-1突变相关的阿尔茨海默病痴呆的合共病：阿尔茨海默病神经病理改变依赖性的证据

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-18 DOI: 10.1093/jnen/nlaf030

引用次数: 0

Digital pathology in tau research: A comparison of QuPath and HALO. 数字病理学在tau研究中的应用：QuPath和HALO的比较。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-16 DOI: 10.1093/jnen/nlaf026

Angelique D Gonzalez, Yannick N Wadop, Benjamin Danner, Kyra M Clarke, Matthew B Dopler, Ali Ghaseminejad-Bandpey, Sahana Babu, Julie Parker-Garza, Cole Corbett, Mohammad Alhneif, Mallory Keating, Kevin F Bieniek, Gladys E Maestre, Sudha Seshadri, Shahroo Etemadmoghadam, Bernard Fongang, Margaret E Flanagan

{"title":"Digital pathology in tau research: A comparison of QuPath and HALO.","authors":"Angelique D Gonzalez, Yannick N Wadop, Benjamin Danner, Kyra M Clarke, Matthew B Dopler, Ali Ghaseminejad-Bandpey, Sahana Babu, Julie Parker-Garza, Cole Corbett, Mohammad Alhneif, Mallory Keating, Kevin F Bieniek, Gladys E Maestre, Sudha Seshadri, Shahroo Etemadmoghadam, Bernard Fongang, Margaret E Flanagan","doi":"10.1093/jnen/nlaf026","DOIUrl":"https://doi.org/10.1093/jnen/nlaf026","url":null,"abstract":"The application of digital pathology tools has expanded in recent years, but non-neoplastic human brain tissue presents unique challenges due to its complexity. This study evaluated HALO and QuPath tau quantification performance in the hippocampus and mid-frontal gyrus across various tauopathies. Percent positivity emerged as the most reliable measure, showing strong correlations with Braak stages and CERAD scores, outperforming object and optical densities. QuPath demonstrated superior correlations with Braak stages, while HALO excelled in aligning with CERAD scoring. However, HALO's optical density was less consistent. Paired t-tests revealed significant differences in object and optical densities between platforms, though percent positivity was consistent across both. QuPath's threshold-based object density showed similar agreement with manual counts compared to HALO's AI-dependent approach (all ρ > 0.70). Reanalysis of QuPath further improved its agreement with manual measurements and correlations with Braak and CERAD scores (all ρ > 0.70). HALO offers a user-friendly interface and excels in certain metrics but is hindered by frequent software malfunctions and more limited flexibility. In contrast, QuPath's customizable workflows and superior performance in Braak staging make it more suitable for advanced and larger-scale analyses. Overall, our study highlights the strengths and limitations of these platforms, helping guide their application in neuropathology.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining dopamine and vitamin E enhances the differentiation to cholinergic neurons of mesenchymal stem cells. 多巴胺与维生素E联用可促进间充质干细胞向胆碱能神经元分化。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-11 DOI: 10.1093/jnen/nlaf025

Ramada R Khaswaneh, Ejlal Abu-El-Rub, Ayman Alzu'bi, Rawan Almazari, Amneh Alrabie, Fatimah A Almahasneh, Amani Kasasbeh, Heba F Ai-Jariri, Ayman Mustafa

{"title":"Combining dopamine and vitamin E enhances the differentiation to cholinergic neurons of mesenchymal stem cells.","authors":"Ramada R Khaswaneh, Ejlal Abu-El-Rub, Ayman Alzu'bi, Rawan Almazari, Amneh Alrabie, Fatimah A Almahasneh, Amani Kasasbeh, Heba F Ai-Jariri, Ayman Mustafa","doi":"10.1093/jnen/nlaf025","DOIUrl":"https://doi.org/10.1093/jnen/nlaf025","url":null,"abstract":"Recent research indicates that mesenchymal stem cells (MSCs) can transdifferentiate into neuron-like cells under specific conditions, offering promise for neuronal regeneration. However, challenges remain in optimizing differentiation protocols to generate specific neuron types. This study explores the impact of supplementing neuronal induction media with dopamine and vitamin E to guide MSCs toward specific neuronal subtypes. Human adipose-MSCs were utilized to investigate neuronal differentiation. The cells were cultured in induction media supplemented with 2 concentrations of dopamine (2.5 and 5 µM) and vitamin E (12.5 and 25 µM). Immunostaining and western blot analysis were employed to assess the sequential expression of neuronal markers associated with various stages of maturation and development. These markers included Nestin, MAP2, NeuN, TBR1, SATB2, DAT, DBH, and CHAT. The results demonstrate, for the first time, that supplementing neuronal induction media with dopamine and vitamin E significantly enhances and accelerates the differentiation of MSCs into neuronal cells. Furthermore, the findings suggest that the induced cells are predominantly reprogrammed toward a cholinergic neuronal lineage. For MSCs, our study reveals that the addition of dopamine and vitamin E reprograms MSCs mainly toward cholinergic neurons, suggesting promising approaches for treating neurodegenerative disorders.","PeriodicalId":16682,"journal":{"name":"Journal of Neuropathology and Experimental Neurology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solitary fibrous tumor-meningioma collision tumor with 18 years of follow-up. 孤立性纤维瘤-脑膜瘤碰撞瘤，随访18年。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-11 DOI: 10.1093/jnen/nlaf037

Phuong Nguyen, Raquel T Yokoda, Quazi Hossain, Joshua B Bederson, Timothy E Richardson

引用次数: 0

Correction to: PTEN Blocking Stimulates Corticospinal and Raphespinal Axonal Regeneration and Promotes Functional Recovery After Spinal Cord Injury. 纠正：PTEN阻断刺激皮质脊髓和raphspinal轴突再生，促进脊髓损伤后功能恢复。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-08 DOI: 10.1093/jnen/nlaf027

引用次数: 0

TBX19/SF1 co-expressing pituitary neuroendocrine tumor/pituitary adenomas add to the lineage infidelity. TBX19/SF1共表达垂体神经内分泌肿瘤/垂体腺瘤增加了谱系不忠。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-08 DOI: 10.1093/jnen/nlaf028

Christie G Turin, Timothy H Ung, B K Kleinschmidt-DeMasters

引用次数: 0

Consecutive brain biopsies illustrate the histological evolution of acute hemorrhagic leukoencephalitis. 连续的脑活检说明急性出血性脑白质炎的组织学演变。

IF 3.2 3区医学

Journal of Neuropathology and Experimental Neurology Pub Date : 2025-04-04 DOI: 10.1093/jnen/nlaf019

Felipe J S Jones, Emily Ling-Lin Pai, Rogan Magee, Kelly Boylan, Vivian Chioma, Jessica Little, Daniel Yoshor, Jose L Pascual, Amir Banihashemi, Sashank Prasad, Edward B Lee, Jennifer Orthmann-Murphy

引用次数: 0