Journal of Parasitology Research最新文献

{"title":"Soil-Transmitted Helminths in Kindergarten Children: Prevalence, Intensity and Associated Factors in Bule Hora Town.","authors":"Wako Dedecha, Tibeso Gemechu, Oliyad Husen, Habtemu Jarso, Lenco Bati","doi":"10.1155/2024/9356919","DOIUrl":"10.1155/2024/9356919","url":null,"abstract":"<p><p><b>Background:</b> Soil-transmitted helminth (STH) infections are a common problem in Ethiopia. This parasite affects the physical and mental development of children, causing malnutrition and iron deficiency anaemia. However, there are inadequate studies that demonstrate the extent of STHs and associated factors among kindergarten children in South Ethiopia, particularly in the study area. <b>Objective:</b> The aim of this study is to determine the prevalence, intensity, and associated factors of STH infection among kindergarten students in Bule Hora town. <b>Methods:</b> An institution-based cross-sectional study was conducted among randomly selected 235 kindergarten children in Bule Hora town from January to February 2023. A pretested questionnaire was used to collect information on associated factors. EpiData and SPSS were used for data entry and analysis, respectively. Binary logistic regression analyses were performed to identify risk factors. <b>Results:</b> In the present study, the prevalence of STH was 28.5% (95% CI: 22.7%-34.3%). <i>A. lumbricoides</i> 26 (11.1%) was the most common parasite identified. The arithmetic mean (+SD) of the egg counts for each species of STHs was as follows: <i>A. Lumbricoides</i> 1886.9 (+2103.2), <i>T. Trichiura</i> 299.2 (+305.8), and hookworms 756.1 (+739.4). Factors that showed significant association in the current study were hand washing after the toilet (AOR: 2.992, 95% CI: 1.299-6.891,), fingernail trimming (AOR: 3.646, 95% CI: 1.704-7.798,), and shoe wearing habit (AOR: 2.143, 95% CI: 1.170-3.924,). <b>Conclusion:</b> STH infection is a health problem among kindergarten children in Bule Hora town. Comprehensive health education on the value of hygienic habits, improved sanitation, and routine deworming of kindergarten children should be included in prevention and control efforts.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"9356919"},"PeriodicalIF":1.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Identification of <i>Hyalomma</i> Ticks and Application of <i>Bacillus thuringiensis</i> Toxins as an Effective Biological Acaricide.","authors":"Panhwer Sana Noor, Munir Ahmed, Abdul Suboor Ansari, Javaid Ali Gadahi, Shahar Bano Memon, Mansoor Tariq, Zubair Ahmed Laghari, Feroza Soomro, Bachal Bhutto, Noor-Un-Nisa Mari, Zhengli Chen","doi":"10.1155/2024/9952738","DOIUrl":"https://doi.org/10.1155/2024/9952738","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;i&gt;Bacillus thuringiensis&lt;/i&gt; (&lt;i&gt;B. thuringiensis&lt;/i&gt;) is considered one of the most important entomopathogenic microorganisms. It produces potent toxins against insects. Therefore, the present study investigates the bioacaricidal properties of &lt;i&gt;B. thuringiensis&lt;/i&gt; on the &lt;i&gt;Hyalomma&lt;/i&gt; tick species. Firstly, we identify &lt;i&gt;Hyalomma&lt;/i&gt; ticks based on morphological screening and molecular characterization. The cytochrome C oxidase subunit I (COX1) gene was selected for the polymerase chain reaction (PCR) analysis, which resulted in the amplification of 656 bp. The amplified products were sequenced, and the isolated (COX1) gene of ticks was submitted to the gene bank of NCBI (Accession No. OR077934.1). The nucleotide sequences were retrieved from the NCBI data bank by BLASTn analysis, which confirmed that all obtained sequences belong to genus &lt;i&gt;Hyalomma&lt;/i&gt;, and multiple alignments confirmed that the sequence of &lt;i&gt;Hyalomma anatolicum&lt;/i&gt; Tandojam-isolate (HA-TJ) 100% aligned with &lt;i&gt;Hyalomma analoticum&lt;/i&gt; KP792577.1, &lt;i&gt;Hyalomma detritum&lt;/i&gt; KP792595.1, &lt;i&gt;Hyalomma excavatum&lt;/i&gt; KX911989.1, and &lt;i&gt;H. excavatum&lt;/i&gt; OQ449693.1. The generated phylogenetic tree confirmed that sequences of HA-TJ COX1 clustered with a single clad of &lt;i&gt;H. analoticum&lt;/i&gt;, &lt;i&gt;H. excavatum&lt;/i&gt;, and &lt;i&gt;H. detritum&lt;/i&gt;. The acaricidal effect of &lt;i&gt;B. thuringiensis&lt;/i&gt; toxins &lt;i&gt;B. thuringiensis&lt;/i&gt; spore crystal mix (&lt;i&gt;Bt&lt;/i&gt;SCM) and &lt;i&gt;B. thuringiensis&lt;/i&gt; crystal proteins (&lt;i&gt;Bt&lt;/i&gt;cps) was evaluated against larvae and adult life stages of &lt;i&gt;Hyalomma&lt;/i&gt; ticks in vitro. We applied &lt;i&gt;Bt&lt;/i&gt;cps and &lt;i&gt;Bt&lt;/i&gt;SCM separately with different concentrations and calculated the mortality percentage. Adult mortality was estimated at the 8th, 10th, 12th, and 15th days posttreatment and larval mortality after 24 h. During treatment of the adult life stage, at first, ticks were immersed in different concentrations of &lt;i&gt;Bt&lt;/i&gt;cps and &lt;i&gt;Bt&lt;/i&gt;SCM for 5 min after the treatments, and the samples were transferred to sterile containers and placed in an incubator with 80% humidity at 23°C. Furthermore, &lt;i&gt;Bt&lt;/i&gt;cps produced the highest mortality on Day 15, 89 ± 1.00% at a concentration of 3000 &lt;i&gt;μ&lt;/i&gt;g/mL, followed by the 12th, 10th, and 8th days produced 83 ± 1.91%, 70 ± 1.15%, and 61 ± 1.00%, respectively. &lt;i&gt;Bt&lt;/i&gt;SCM produced mortality of 69 ± 1.91% on Day 15 at a concentration of 3000 &lt;i&gt;μ&lt;/i&gt;g/mL, followed by the 12th, 10th, and 8th days at 57 ± 2.51%, 37 ± 1.91%, and 34 ± 2.00%. The present study revealed that &lt;i&gt;B. thuringiensis&lt;/i&gt; toxins produced a significant (&lt;i&gt;p&lt;/i&gt; &lt; 0.05) increase in mortality rate in adults of &lt;i&gt;Hyalomma&lt;/i&gt; ticks. Additionally, &lt;i&gt;Bt&lt;/i&gt;cps and &lt;i&gt;Bt&lt;/i&gt;SCM were used to treat the larval stage. The treatments were applied to calculate the mortality percentage via the Laravel packet test. At a 1500 &lt;i&gt;μ&lt;/i&gt;g/mL concentration, &lt;i&gt;Bt&lt;/i&gt;cps resulted in the highest mortality of 98 ± 1.15%; this was followed by 1250 &lt;i&gt;μ&lt;/i&gt;g/mL, 1000 &lt;i&gt;μ&lt;/i&gt;","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"9952738"},"PeriodicalIF":1.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11410401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro and In Silico Anthelmintic Activity of Extracts of <i>Lannea kerstingii</i> and <i>Ficus thonningii</i> on <i>Heligmosomoides polygyrus</i>.","authors":"Ndjinkeu Ntcheuzing Serena, Masoud Besati, Noumedem Anangmo Christelle Nadia, Mahdi Yaghoobi, Yamssi Cédric, Claire Ciancia, Ngouyamsa Nsapkain Aboubakar Sidiki, Vincent Khan Payne, Mpoame Mbida, Haibo Hu","doi":"10.1155/2024/1858154","DOIUrl":"10.1155/2024/1858154","url":null,"abstract":"<p><p><b>Background:</b> The aim of this study was to assess the anthelmintic activity of <i>Lannea kerstingii</i> and <i>Ficus thonningii</i>, on a nematode model, to promote their use in the Cameroonian pharmacopoeia for the treatment of helminthiases. <b>Methods:</b> One nematode was used, <i>Heligmosomoides polygyrus</i>. First, the effect of the extracts on the eggs and larval stages (L1, L2, and L3) of <i>H. polygyrus</i> was evaluated, 100 <i>μ</i>L of extract and 100 <i>μ</i>L of parasite suspension (containing 50 eggs) were mixed in a 96-well microplate. The 96-well microplate was incubated for 20 h at 25°C in the WMicroTracker which measures the motility of the worms at various concentrations. Finally, docking studies were conducted by using the Glide module in Schrodinger Maestro. <b>Results:</b> The ethanolic extract of <i>L. kerstingii</i> with the half maximal inhibitory concentration (IC₅₀) of 0.1371 mg/mL produced a higher ovicidal effect than the effect produced by other extracts of these plants. However, with an IC₅₀ of 0.31 mg/mL, the aqueous extract of <i>F. thonningii</i> showed the greatest effect on the L2 stage. The aqueous and ethanolic extracts of <i>L. kerstingii</i> and <i>F. thonningii</i> inhibited the development of the L3 larvae of <i>H. polygyrus</i> with a better effect for the ethanolic extracts. <b>Conclusion:</b> The use of <i>L. kerstingii</i> and <i>F. thonningii</i> for the treatment of helminthiasis has been proved in vitro and in silico by this research. However, more research is required, especially on the acute toxicity and in vivo anthelmintic efficacy to validate this scientific investigation.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"1858154"},"PeriodicalIF":1.4,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11316912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Analysis of the ROP29 Protein as a Vaccine Candidate Against <i>Toxoplasma gondii</i>.","authors":"Amir Karimipour-Saryazdi, Fatemeh Ghaffarifar, Abdolhossein Dalimi, Masoud Foroutan, John Horton, Javid Sadraei","doi":"10.1155/2024/1918202","DOIUrl":"10.1155/2024/1918202","url":null,"abstract":"<p><p>The progression of <i>Toxoplasma gondii</i> (<i>T. gondii</i>) invasion is aided by rhoptry proteins (ROPs), which are also crucial for the parasite's survival in host cells. In this study, in silico analysis was performed to examine the various aspects of the ROP29 protein, such as physicochemical properties, potential T- and B-cell epitopes, and other significant features. The research revealed that there were 55 possible sites for posttranslational modification in the ROP29 protein. The secondary structure of the ROP29 protein consists of a random coil, an alpha-helix, and an extended strand, which account for 49.69%, 36.81%, and 13.50%, respectively. Moreover, a number of putative T- and B-cell epitopes for ROP29 were found. The Ramachandran plot showed that 88.91% (crude model) and 97.54% (refine model) of the amino acid residues were located in the favored regions. Also, the testing of this protein's antigenicity and allergenicity showed that it was nonallergenic and immunogenic. Our results suggested that employing in silico tools to apply structural and functional predictions to the ROP29 protein can lower the likelihood that laboratory investigations will fail. This research served as a crucial foundation for further research. More research is required in the future in suitable animal model employing ROP29 alone or in combination with other antigens.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"1918202"},"PeriodicalIF":1.4,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoonotic Parasites and Their Association With Human Activities in Northern Tanzania: An Integrated Ecosystem Approach for One Health.","authors":"Barakaeli Abdieli Ndossi, Eblate Ernest Mjingo, Hansol Park, Dongmin Lee, Mohammed Mebarek Bia, Heejae Yang, Sungbo Seo, Keeseon S Eom","doi":"10.1155/2024/8872837","DOIUrl":"10.1155/2024/8872837","url":null,"abstract":"<p><p>The community's awareness of the prevalence and impact of zoonotic diseases has been significantly underestimated, leading to insufficient implementation of control measures. This study was carried out in Northern Tanzania between 2019 and 2023 to investigate zoonotic parasites and the risks associated with human activities that contribute to zoonotic diseases. Cross-sectional surveys were conducted in 12 villages, including nine in Loliondo Division and three in Babati District. Focus Group Discussions and Key Informant Interviews were conducted to assess the community's knowledge and practices regarding the risks associated with zoonotic diseases in the surveyed areas. A total of 255 samples were collected from various sources, including latrines, households, livestock enclosures, domestic dogs, and chickens. Out of these samples, 152 tested positive for identifiable parasite eggs and oocysts. These parasites included hookworms (21.7%), <i>Trichuris</i> sp. (14.5%), <i>Strongyloides</i> sp. (13.8%), <i>Eimeria</i> sp. (19.7%), Taeniids (5.9%), <i>Hymenolepis</i> sp. (3.3%), <i>Spirometra</i> sp. (2.6%), and <i>Dipylidium</i> sp. (0.7%). Taeniids and <i>Spirometra</i> species were predominantly found in villages near protected areas such as Arash Sokoni, Oloipiri, Sukenya, Wasso, Orkuyiene, Haytemba, and Loliondo. Hookworms were most commonly detected in Arash Sokoni, Loliondo, Isuguro, and Hyatemba, while <i>Strongyloides</i> sp. was prevalent in Wasso, Sukenya, and Olobo villages. The quantitative analysis reveals significant associations between hygiene practices, proximity to livestock enclosures, ecological factors, and human-animal interaction, highlighting their pivotal roles in determining soil-transmitted helminth (STH) prevalence across different villages. This study reveals that there was a generally low level of awareness regarding zoonotic diseases and STHs. The detection of STH samples, combined with the limited understanding of zoonotic diseases, emphasizes the importance of taking proactive measures to reduce transmission risks. Prioritizing education and promoting awareness along with implementing comprehensive strategies are essential steps to effectively tackle the problems linked to STH infections and substantially lessen the public health burden caused by zoonotic diseases.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"8872837"},"PeriodicalIF":1.4,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11281853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helminth Parasite Prevalence in the Endangered Ethiopian Wolf (<i>Canis simensis</i>) in Web Valley, Bale Mountains National Park, Ethiopia.","authors":"Girma Ayalew Mengistu, Nigatu Kebede, Fedlu Abdella","doi":"10.1155/2024/6057393","DOIUrl":"10.1155/2024/6057393","url":null,"abstract":"<p><p>Ethiopian wolves (EWs), <i>Canis simensis</i>, are the rarest canids in the world and Africa's most endangered carnivore, found in only six isolated habitat fragments in the highlands of Ethiopia. Previous reports on the prevalence of parasites in the EW in Bale Mountains National Park (BMNP) are limited, with little information on their helminth fauna. This study seeks to understand the prevalence of helminth parasites in the EW in BMNP, Ethiopia. In this study, fecal samples were collected from 43 EWs in Web Valley (WV), BMNP, from June to October 2020, and the presence of helminth eggs was assessed using fecal sedimentation and centrifugal floatation methods with microscopy. Forty-two out of 43 fecal samples from wolves (98%) contained eggs from two taxonomic groups of helminths. Eggs from <i>Capillaria</i> spp. and <i>Trichuris vulpis</i> were found most frequently, followed by <i>Toxocara canis</i>, <i>Diphyllobothrium</i> spp., <i>Toxascaris leonina</i>, and <i>Capillaria aerophila</i>. One EW (2%) was recorded for harboring the cestode <i>Moniezia expansa</i>. About 9 of the 43 EWs (21%) presented monospecific infection: 9 EWs (21%) harbored 2 parasite species, 9 EWs (21%) hosted 3 parasite species, 11 EWs (26%) had infection involving 4 parasite species, 2 EWs (5%) were infected with 5 parasite species, 1 EW (2%) presented 6 parasite species, 1 EW (2%) harbored 7 parasite species, and 1 EW (2%) was diagnosed without parasite species. Concurrent helminth infection was highly associated with female EW. Megeti 3 was associated with a low level of concurrent helminth infection. The prevalence of helminth parasites found in wolves in the study area suggests that the environment is highly contaminated with intestinal parasites. Regular control of parasite transmission in EW, domestic dogs, and humans in and around BMNP, public education, and further parasite epidemiological studies must be conducted.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"6057393"},"PeriodicalIF":1.4,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11226338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Protocol to <i>Toxoplasma gondii</i> Detection in Fresh Goat Milk.","authors":"Igor Falco Arruda, Patricia Riddell Millar, Mário Felipe Alvarez Balaro, Thamires Francisco Bonifácio, Raissa Cristina Ferreira Ramos, Maria Regina Reis Amendoeira","doi":"10.1155/2024/6895089","DOIUrl":"https://doi.org/10.1155/2024/6895089","url":null,"abstract":"<p><p><i>Toxoplasma gondii</i> is a zoonotic parasite with global distribution capable of infecting homeothermic animals. Transmission of protozoan to humans includes ingestion of water and raw food contaminated with sporulated oocysts, ingestion of raw or undercooked meat with tissue cysts, and tachyzoites' transplacental transmission. Fresh goat milk intake has already been linked to human toxoplasmosis outbreaks, but little is known about the infectious potential of this biological sample. Accordingly, the aim of the present study is to assess the survival and infectivity of <i>T. gondii</i> tachyzoites in fresh goat milk samples through an experimental protocol to detect this parasite via bioassay carried out with a murine model, DNA amplification, and serology. Swiss Webster mice were inoculated with fresh goat milk samples contaminated with different <i>T. gondii</i> RH strain tachyzoite concentrations per milliliter and stored for different refrigeration times. Animals showing clinical signs compatible to toxoplasmosis were euthanized. Milk samples contaminated with high parasitic loads and kept for a shorter refrigeration time were the most lethal ones. No significant differences were observed between mean death rates recorded for different goat milk contamination concentrations (<i>p</i> = 0.1888), and for the refrigeration time, contaminated milk samples were kept under (<i>p</i> = 0.9440). <i>T. gondii</i> DNA was amplified in all contaminated milk samples, but only one of the surviving mice was serologically positive. Results of the present study have shown <i>T. gondii</i> survival and infectivity in fresh goat milk samples, and it highlights its significant risk for public health. Therefore, molecular methods must be the tests of choice when milk samples are used to assess infection caused by protozoan in goats' dairy products.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"6895089"},"PeriodicalIF":1.4,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11458309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trypanosome Infection in Cattle and Associated Vectors in Etang District of Gambella, Ethiopia.","authors":"Negesa Tola, Akinaw Wagari, Geremew Haile Lemu, Mohamed Kedir, Haben Fesseha Gebremeskel, Isayas Asefa Kebede","doi":"10.1155/2024/5548718","DOIUrl":"10.1155/2024/5548718","url":null,"abstract":"<p><strong>Background: </strong>Bovine trypanosomosis produces significant economic losses due to anemia, loss of body condition, and emaciation. The disease is transmitted biologically by tsetse flies and mechanically by biting flies such as <i>Tabanus</i> and <i>Stomoxys</i>. Therefore, this research is aimed at estimating the prevalence of bovine trypanosomosis and the apparent density of its vectors in the Etang Special District.</p><p><strong>Methods: </strong>A cross-sectional study was employed from November 2016 to April 2017 for the prevalence and entomological survey. Trypanosoma species were identified using buffy coat and Giemsa staining techniques. Besides, the entomological surveys were conducted using NGU, pyramidal, biconical, and monoconical traps. The vectors were identified to their genus level based on their morphological features like size, color, wing venation, and proboscis.</p><p><strong>Result: </strong>A total of 457 bovine blood samples were collected and tested, of these 16 (3.50%) animals were positive for trypanosomosis. Similarly, 13 <i>T. vivax</i> (81.25%) and 3 <i>T. congolense</i> (18.75%) were the trypanosome species detected. The prevalence in the young (2.56%) and adult (3.99%) age groups was not statistically significant (<i>P</i> > 0.05). However, there were significant (<i>P</i> < 0.05) differences in prevalence between body condition scores, where poor is 6.31%, medium is 1.30%, and good is 0%. Moreover, the difference in mean PCV values between the parasitemic (20.97) and aparasitemic (28.58) groups was statistically significant (<i>P</i> < 0.05). <i>Glossina</i> flies were not found, although 1756 <i>Tabanus</i> and 52 <i>Stomoxys</i> biting flies were gathered. Accordingly, the overall apparent density of <i>Glossina</i> flies was zero (0), with biting flies (20.54) recorded per trap per day. Moreover, the apparent density of <i>Tabanus</i> and 52 <i>Stomoxys</i> was 39.01 and 1.18, respectively.</p><p><strong>Conclusion: </strong>This study confirmed that trypanosomosis and abundant mechanical vectors continue to be problems in the study area, resulting in cattle productivity losses. As a result, strategic management and prevention methods for trypanosomosis and associated vectors should be prioritized. Further investigation of vector needs to be conducted to clear out tsetse presence.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"5548718"},"PeriodicalIF":1.4,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11323987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141982500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Determination of <i>Toxocara</i> spp. Eggs Isolated from Public Parks and Playgrounds in Zahedan, Southeast Iran.","authors":"Siavash Liravizadeh, Samaneh Abdolahi Khabisi, Alireza Salimi Khorashad, Hadi Mirahmadi","doi":"10.1155/2024/2132696","DOIUrl":"10.1155/2024/2132696","url":null,"abstract":"<p><strong>Background: </strong>Human toxocariasis (HT) is a zoonotic disease with a global expansion. Contaminated soil with <i>Toxocara</i> spp. eggs is the main source of human infection, which may lead to severe complications depending on the organs invaded by migrating larvae.</p><p><strong>Aim: </strong>This study is aimed at eliciting the prevalence of <i>Toxocara</i> spp. eggs in public parks in Zahedan, southeast Iran, and providing new insight into the soil contamination rate in this area using microscopic and molecular methods.</p><p><strong>Methods: </strong>Based on five municipal districts, 240 soil samples were collected from public parks and playgrounds in Zahedan. The modified Sheather's flotation technique was employed to isolate <i>Toxocara</i> spp. eggs from the soil, followed by microscopic assessment and molecular evaluation of internal transcribed spacer 1 and 2 ribosomal deoxyribonucleic acid (ITS1 and 2 rDNA) using nested polymerase chain reaction (nested PCR) to identify the presence of <i>Toxocara</i> spp. eggs. The Sanger sequence was used to differentiate the <i>Toxocara</i> species. Subsequently, all the sequenced data were blasted and compared with other sequences available in the GenBank.</p><p><strong>Results: </strong>Out of 240 soil samples collected, 7 (2.9%) samples were identified to contain <i>Toxocara</i> spp. eggs using Sheather's flotation and microscopic techniques. Meanwhile, 19 (7.9%) samples were positive using nested PCR. According to the Sanger sequencing analysis findings, all positive samples were contaminated with <i>Toxocara cati</i>.</p><p><strong>Conclusion: </strong>As evidenced by the obtained results, only <i>T. cati</i> species were detected in public parks and playgrounds in Zahedan; therefore, control and prevention programs against this species should be considered in human and animal communities.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"2132696"},"PeriodicalIF":1.4,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11208816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Intestinal Parasitic Infections and Associated Factors among Presumptive Pulmonary Tuberculosis Patients at Debre Tabor Referral Hospital, South Gondar, Northwest Ethiopia: A Cross-Sectional Study.","authors":"Debaka Belete, Azanaw Amare, Tesfaye Andualem, Desie Kasew, Sirak Biset","doi":"10.1155/2024/8993666","DOIUrl":"10.1155/2024/8993666","url":null,"abstract":"<p><strong>Background: </strong>In developing countries, intestinal parasitic infections (IPIs) and tuberculosis (TB) coinfections have been perceived to be high. The geographic distributions of helminths and TB overlap substantially. Parasitic infections affect the outcome of TB by changing the cell-mediated immune response to a humoral response, while <i>Mycobacterium</i> infection favors the immune escape of helminths. There are limited studies on the epidemiology of intestinal parasites among presumptive pulmonary TB (PTB) patients in Ethiopia. Therefore, this study is aimed at determining the prevalence of intestinal parasitic infections and associated factors among patients with presumptive pulmonary tuberculosis at Debre Tabor Referral Hospital.</p><p><strong>Methods and materials: </strong>A hospital-based cross-sectional study was conducted from March to June 2021. The sociodemographic data and associated factors were collected using a structured questionnaire, and stool samples were collected by convenient sampling technique and processed for the detection of intestinal parasites using a direct wet mount saline preparation and formal ether concentration technique. The data was coded, cleaned, and analyzed by SPSS version 23. Bivariate and multivariable analyses were conducted to determine an adjusted odds ratio (AOR). <i>p</i> value < 0.05 was considered statistically significant.</p><p><strong>Result: </strong>The overall prevalence of intestinal parasitosis was 25.6% (81/316); of these, 12.9% (41/316) were protozoan infections and 12.7% (40/316) were helminth infections. Multivariable logistic regression analysis showed that being older than 36 years (AOR: 4.35; 95% CI: 1.26, 13.91; <i>p</i> = 0.001), rural residence (AOR: 3.46; 95% CI: 1.18, 9.97; <i>p</i> < 0.001), unable to read and write (AOR = 2.62; 95%CI = 2.15, 8.43; <i>p</i> = 0.004), and use of river water (AOR: 3.47; 95% CI: 1.62, 8.21; <i>p</i> < 0.001) were associated with intestinal parasitic infections.</p><p><strong>Conclusion: </strong>The present study showed that the prevalence of intestinal parasitosis among presumptive pulmonary tuberculosis patients was high in the study area. Age, residence, educational status, and source of water were significant factors in IPIs among presumptive TB patients. Moreover, our findings suggest a proper health education program for good personal hygiene habits, the coloration of water, avoiding open-field defecation, and also preventative measures to avoid the acquisition of IPIs in patients with TB. Presumptive tuberculosis patients should be screened and treated accordingly. Additionally, it needs further research and recommends more assessment for intestinal parasitic infection in PTB patients.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2024 ","pages":"8993666"},"PeriodicalIF":2.2,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11127764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}