Journal of Orthopaedic Surgery and Research最新文献

{"title":"Intra-articular injection of vancomycin after arthrotomy closure following gentamicin-impregnated bone cementation in primary total knee arthroplasty provides a high intra-articular concentration while avoiding systemic toxicity: a prospective study.","authors":"Xuwen Li, Junhao Lai, Xue Yang, Hao Xu, Shuai Xiang","doi":"10.1186/s13018-024-05357-9","DOIUrl":"10.1186/s13018-024-05357-9","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to elucidate the safety and intra-articular elution profiles of vancomycin and gentamicin bone cement in patients undergoing primary total knee arthroplasty (TKA), with a focus on serum safety thresholds and therapeutic efficacy.</p><p><strong>Methods: </strong>Consecutive patients who underwent unilateral primary TKA were prospectively enrolled. The implants were fixed using gentamicin-impregnated bone cement, and after arthrotomy closure, 1000 mg of vancomycin suspended in 25 mL of normal saline was directly injected into the joint. Peripheral venous blood and drain fluid samples were collected 2, 8, and 24 h postoperatively. The serum and intra-articular concentrations of vancomycin and gentamicin were analyzed using liquid chromatography-tandem mass spectrometry within 24 h.</p><p><strong>Results: </strong>Clinical data reflecting renal and liver function were recorded preoperatively, and at 24 and 72 h postoperatively. A total of 100 patients were included. At 2, 8, and 24 h postoperatively, the serum vancomycin concentration was 7.0 ± 2.0, 5.7 ± 1.8, and 3.6 ± 1.4 µg/mL, respectively, while the intra-articular concentration was 468.5 (interquartile range [IQR] 286.0 to 774.8), 139.5 (IQR 52.0 to 295.3), and 34.4 (IQR 22.2 to 56.8) µg/mL, respectively; 33.2 (IQR 19.5 to 80.5) mg vancomycin was lost in drainage fluid at 24 h postoperatively. For gentamicin, the overall intra-articular concentration was 70.4 (IQR 35.4 to 109.2), 33.8 (IQR 17.8 to 73.9), and 21.1 (IQR 12.2 to 36.0) µg/mL at 2, 8, and 24 h postoperatively, respectively, with an undetectable serum concentration. No cases of acute renal injury, liver injury, ototoxicity, or anaphylaxis were observed.</p><p><strong>Conclusions: </strong>Intra-articular injection of 1000 mg vancomycin after arthrotomy closure combined with gentamicin-impregnated bone cement provided a therapeutic intra-articular concentration while avoiding systemic toxicity over the initial 24 h after primary TKA. Therefore, intra-articular vancomycin administration may offer a safer alternative to intravenous antibiotics, reducing systemic toxicity; however, further large-scale studies are necessary.</p><p><strong>Trial registration: </strong>ClinicalTrials. Gov (registration number: NCT05338021).</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"856"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of MRI-based VBQ and EBQ score for predicting cage subsidence in PILF surgery.","authors":"Fan Zhang, Jie Liang, Dawei Shi, Can Tuo, Yu Wu, Zijian Yang","doi":"10.1186/s13018-024-05332-4","DOIUrl":"10.1186/s13018-024-05332-4","url":null,"abstract":"<p><strong>Background context: </strong>As lumbar degenerative diseases become more prevalent in an aging population, there is an increasing demand for surgical interventions, such as posterior lumbar interbody fusion (PLIF). However, cage subsidence (CS), observed in 23.9-54% of cases postoperatively, remains a significant complication. Several factors, including age, bone quality, and endplate damage, contribute to the risk of CS, with bone quality being among the most critical determinants. Although DEXA and QCT are widely employed to assess bone density, their routine use in preoperative evaluations is restricted by cost considerations and radiation exposure. Recent studies suggest that MRI-based vertebral body quality (VBQ) and endplate bone quality (EBQ) score offer a viable, non-invasive alternative for evaluating bone quality; however, there is limited research comparing their predictive value for CS.</p><p><strong>Methods: </strong>In this retrospective study, 165 patients undergoing single-level PLIF surgery were included. MRI-based VBQ and EBQ score were calculated using T1-weighted images, and preoperative QCT was employed as a clinical standard. Cage subsidence was assessed based on postoperative imaging at 12-month follow-up. Statistical analyses, including t-tests, chi-square tests, and ROC curve analyses, were used to evaluate the predictive accuracy of VBQ and EBQ for CS.</p><p><strong>Results: </strong>The study's findings demonstrated that both VBQ and EBQ scores were significantly correlated with QCT measurements, thereby validating their utility as indicators of bone quality. ROC analysis revealed that VBQ had superior predictive value for CS (AUC = 0.814) compared to EBQ (AUC = 0.719), with both scores demonstrating significant clinical utility in identifying patients at risk for CS. Notably, VBQ exhibited a stronger correlation with preoperative clinical outcomes compared to EBQ, underscoring its greater reliability as a predictor.</p><p><strong>Conclusions: </strong>This study highlights the effectiveness of MRI-based VBQ and EBQ score as practical, non-invasive tools for assessing bone quality preoperatively, with VBQ demonstrating superior predictive performance for CS risk. The findings underscore the potential of integrating these MRI-based assessments into routine preoperative planning to improve patient outcomes and minimize complications associated with PLIF surgery.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"839"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in perioperative pain management for total knee arthroplasty: a review of multimodal analgesic approaches.","authors":"Chengcheng Zhao, Qimeng Liao, Dinglong Yang, Mingyi Yang, Peng Xu","doi":"10.1186/s13018-024-05324-4","DOIUrl":"10.1186/s13018-024-05324-4","url":null,"abstract":"<p><p>Despite significant advancements in total knee arthroplasty (TKA), managing postoperative pain remains a substantial clinical challenge. With advances in surgical techniques and pharmacology, TKA perioperative analgesic strategies continue to evolve, necessitating ongoing reassessment of current data. Multimodal analgesia has become the standard for perioperative pain management in TKA; however, variations in its specific components and applications across studies create challenges in selecting the optimal analgesic approach. Evaluating these pain management strategies is essential for understanding their benefits, limitations, and appropriate use, allowing the development of individualized perioperative analgesic plans. This review aims to summarize current research on perioperative pain control in TKA and assess the effectiveness of different analgesic interventions.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"843"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the efficacy of intramedullary nailing via the lateral parapatellar approach versus the infrapatellar approach in the treatment of tibial metaphyseal-diaphyseal junction fractures.","authors":"Xin Cao, Qingxiang Tang, Bingxin Zhou, Wei Xiao, Huijin Chen","doi":"10.1186/s13018-024-05338-y","DOIUrl":"10.1186/s13018-024-05338-y","url":null,"abstract":"<p><strong>Background: </strong>To compare the efficacy of intramedullary nailing via the lateral parapatellar approach versus the infrapatellar approach in treating fractures at the tibial metaphyseal-diaphyseal junction.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on the clinical data of 45 patients with proximal or distal tibial fractures treated with intramedullary nailing via lateral parapatellar approach (n = 23) or infrapatellar approach (n = 22) between January 2019 and March 2023. We recorded and compared the operative time, intraoperative blood loss/fluoroscopies, success rate of closed reduction, anteroposterior and lateral entry point accuracy, postoperative infection, fracture healing time, as well as NRS pain scores, Lysholm knee function scores, and knee range of motion.</p><p><strong>Results: </strong>Both groups completed the surgery without any complications. The lateral parapatellar approach group had significantly better results regarding shorter operative time, less intraoperative blood loss, and fewer intraoperative fluoroscopies compared to the infrapatellar approach group (P < 0.05). All cases in the lateral parapatellar approach group achieved closed reduction, while 10 cases in the infrapatellar approach group required open reduction. Fractures in both groups healed successfully, without statistically difference in healing time (P > 0.05). The accuracy of anteroposterior and lateral entry points was better with lateral parapatellar approach (P < 0.05). At 3 and 12 months postoperatively, lateral parapatellar approach showed better Lysholm and NRS scores compared to infrapatellar approach (P < 0.05). Two groups had no significant difference in range of motion (P > 0.05).</p><p><strong>Conclusions: </strong>Lateral parapatellar approach combined with the blocking screw technique provides superior clinical outcomes compared to infrapatellar approach in the treatment of proximal or distal tibial fractures, making it suitable for further investigation.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"838"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimated direct medical cost of orthopaedic trauma surgery in Saudi Arabia: a single-centre retrospective cost analysis.","authors":"Waleed Albishi, Amjad Albaroudi, Abdullah Addar, Hisham Alsanawi, Abdulrahman Alaseem","doi":"10.1186/s13018-024-05333-3","DOIUrl":"10.1186/s13018-024-05333-3","url":null,"abstract":"<p><strong>Background: </strong>Physical trauma is a significant contributor to morbidity and mortality worldwide. In Saudi Arabia, RTAs are the leading cause of injuries, with fractures being a critical aspect of trauma care. To address this prevalent healthcare burden, a detailed analysis of the costs associated with surgical interventions to optimize resource allocation and improve healthcare planning is crucial.</p><p><strong>Method: </strong>This retrospective cost analysis was conducted at King Saud University Medical City between January 2016 and March 2022. A total of 2689 orthopaedic trauma surgeries were analysed. The cost data were converted to US dollars (1 USD = 3.75 SR). Statistical analysis was conducted via SPSS, with comparisons of costs by sex, age group, procedure type, and other relevant factors.</p><p><strong>Results: </strong>The total cost of trauma surgeries over the study period was $8,548,749.90. The most common procedure was ORIF, which was performed 1415 times, accounting for $5,660,000.00 of the total cost. Males represented 65.7% of the patients, incurring $5,647,976.44 in expenses. Patients under the age of 21 had the highest number of cases by age (43.5%), and the total cost was $3,276,293.44. Scheduled surgeries had higher average costs than emergent procedures did, and lower extremity surgeries accounted for more cases but had lower average costs than upper extremity surgeries did.</p><p><strong>Conclusion: </strong>The cost of orthopaedic trauma care is significantly influenced by factors such as procedure type, age, and sex. When certain cost patterns are identified and analysed, an approach toward cost effectiveness while maintaining high-quality healthcare delivery can be achieved. Projected trends show an increase in the number of cases by 2030, indicating increased costs. Future studies should consider additional factors, such as rehabilitation costs, and explore cost-saving opportunities in surgical interventions.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"837"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obesity and hyperlipidemia were associated with more severe synovitis and structural abnormalities as well as inferior functional outcomes in knee osteoarthritis: a retrospective comparative study.","authors":"Kuo Hao, Juncai Wang, Yingzhen Niu, Fei Wang","doi":"10.1186/s13018-024-05326-2","DOIUrl":"10.1186/s13018-024-05326-2","url":null,"abstract":"<p><strong>Background: </strong>Considering that the respective effects of obesity and hyperlipidemia on knee osteoarthritis (OA) have not been fully investigated, the purpose of this study was to determine the relationship of obesity or hyperlipidemia with the synovitis and structural abnormalities of knee OA, and the effect of obesity and hyperlipidemia on functional outcomes of total knee arthroplasty.</p><p><strong>Methods: </strong>There were 99 OA patients without obesity and hyperlipidemia in Group 1, 100 OA patients only with obesity in Group 2, 98 OA patients only with hyperlipidemia in Group 3, and 97 OA patients with both obesity and hyperlipidemia in Group 4. Semi-quantitative synovial inflammatory markers were measured including effusion-synovitis, size and intensity of infrapatellar fat pad abnormality, and synovial proliferation score. The structural abnormalities of knee OA were evaluated using Whole-Organ Magnetic Resonance Imaging Score (WORMS). Functional outcomes were evaluated before surgery and at 2 years follow-up.</p><p><strong>Results: </strong>There were significantly higher effusion-synovitis, size and intensity of infrapatellar fat pad abnormality, and synovial proliferation score, as well as higher cartilage, bone marrow edema, meniscus, and total WORMS scores in Group 2, Group 3, and Group 4 (P < 0.05), but with no significant difference between Group 2 and Group 3 (P > 0.05). Group 2, Group 3, Group 4 had significantly worse Western Ontario and McMaster Universities Osteoarthritis Index, Forgotten Joint Score, Oxford Knee Score, Knee Society Score at baseline and 2 years follow-up (P < 0.05), but with no significant difference between Group 2 and Group 3 (P > 0.05). There were significant associations of obesity or hyperlipidemia with all synovial inflammatory markers and cartilage, bone marrow edema, meniscus, and total WORMS scores as well as functional outcomes (P < 0.05).</p><p><strong>Conclusions: </strong>Obesity and hyperlipidemia were associated with more severe synovitis and structural abnormalities of knee OA, as well as inferior preoperative and postoperative functional outcomes. The negative effects of obesity and hyperlipidemia on knee OA could be mutually enhanced. The findings emphasized the negative effects of obesity and hyperlipidemia on the symptoms and outcomes of knee OA, and highlighted the association of obesity and hyperlipidemia with synovitis.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"845"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Hybrid closing-wedge distal tuberosity tibial osteotomy using patient-specific instrumentation (PSI) in post-fracture malunion. A technical note.","authors":"Teruyuki Miyasaka, Tomohiro Kayama, Toshiyuki Omori, Rubi Shimokata, Mitsuru Saito","doi":"10.1186/s13018-024-05364-w","DOIUrl":"10.1186/s13018-024-05364-w","url":null,"abstract":"","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"852"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the past status and future tendency: a comprehensive scientometric study on developmental dysplasia of the hip.","authors":"Boshen Shu, Shufeng Zhang, Jian Gao, Lin Wang, Xiaohui Wang","doi":"10.1186/s13018-024-05358-8","DOIUrl":"10.1186/s13018-024-05358-8","url":null,"abstract":"<p><strong>Background: </strong>Developmental dysplasia of the hip is a common musculoskeletal disorder in newborns and young children, attracting considerable controversy. The purpose of the present study was to analyze the research trends and hotspots over the past three decades in this field.</p><p><strong>Methods: </strong>All related publications were collected from the Web of Science Core Collection from 1994 to 2023. Of these, characteristics of the top 50 cited articles for each decade (1994-2003, 2004-2013 and 2014-2023) were evaluated in detail. Visualized studies were operated via VOSviewer. GraphPad Prism and Microsoft Excel were used to perform statistical analysis. The characteristic of the present study is a cross-sectional study.</p><p><strong>Results: </strong>In total, 1,300 publications with 19,815 citations from 1994 to 2023 were selected. The number of publications increased significantly during the past three decades, from 23 in 1994 to 132 in 2023 (P < 0.0001). Studies with collaboration among institutions have raised significantly over time (P < 0.0001). The United States was the leading country to the top 50 publications in each decade. Publications with evidence level IV led the way every ten years. Visualized co-occurrence analysis clustered the keywords into four clusters: hip arthroplasty, congenital dislocation, ultrasound diagnosis and developmental dysplasia of the hip.</p><p><strong>Conclusions: </strong>This study analyzed the global tendency and collaborations on developmental dysplasia of the hip over the past three decades. Even though the research trend has evolved to be collaborative with more multi-institutional publications and authors, studies with high evidence levels are still missing.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"853"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles from platelet-poor plasma possess anti-inflammatory and anti-catabolic effects in chondrocytes stimulated with IL-1β or synovial membrane-conditioned media.","authors":"Slavomira Gulova, Lucia Slovinska, Livia K Fecskeova, Jana Bzdilova, Jana Matejova, Marko Moravek, Marek Lacko, Denisa Harvanova","doi":"10.1186/s13018-024-05355-x","DOIUrl":"10.1186/s13018-024-05355-x","url":null,"abstract":"<p><strong>Background: </strong>Although osteoarthritis (OA) is the most prevalent form of arthritis, there is still no effective treatment capable of combining immunomodulatory effects with cartilage repair. Extracellular vesicles (EVs) represent a promising new generation of cell-free therapies for OA. Blood-derived products, including plasma, are an easily available and abundant source of EVs with anti-inflammatory and regenerative properties. In this study, our objective was to analyze the effect of platelet poor plasma-derived extracellular vesicles (PPP-EVs) on stimulated OA chondrocytes in vitro. We hypothesize that PPP from healthy donors could be a suitable source of EVs that can modulate the inflammatory environment of OA chondrocytes.</p><p><strong>Methods: </strong>Cartilage and synovial membrane (SM) were obtained from patients with OA and whole blood from healthy donors. Synovial membrane-conditioned media (CM / SM) was analyzed using multiplex immunoassays. EVs were isolated from PPP using size exclusion chromatography (SEC) and characterized by nanoparticle tracking analysis (NTA), Western blot, and flow cytometry (FC). The phenotype of the chondrocytes was analyzed using fluorescence microscopy and RT-qPCR. Chondrocytes were stimulated with IL-1β or CM/SM for 24 h. The impact of PPP-EVs on stimulated chondrocyte gene expression was evaluated using RT-qPCR.</p><p><strong>Results: </strong>The PPP-EVs isolated by SEC were positive for the tetraspanins CD9, CD63, and CD81. The chondrocyte phenotype was confirmed by positive expression of Collagen II and Aggrecane. CM/SM and IL-1β caused inflammatory changes in chondrocytes, which was observed by increased expression of the genes MMP-1, MMP-3 and MMP-13, RANTES, TSG-6, and YKL-40 compared to the control. PPP-EVs added to stimulated chondrocytes for 24 h significantly decreased the expression of the chondrocyte gene YKL-40, TSG-6 and MMP-1.</p><p><strong>Conclusions: </strong>In this study, we confirmed that PPP is a suitable source of EVs, which can be efficiently isolated by SEC. We found that PPP-EVs were capable of decreasing the expression of inflammatory genes in OA chondrocytes stimulated with IL-1β or CM/SM. This study provides preliminary results on PPP-EVs as an affordable and promising option to modulate the catabolic microenvironment of OA chondrocytes in vitro.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"847"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"iTRAQ proteomic analysis of exosomes derived from synovial fluid reveals disease patterns and potential biomarkers of Osteoarthritis.","authors":"Xiaomin Wu, Huaiming Li, Fengzhen Meng, Tun Hing Lui, Xiaohua Pan","doi":"10.1186/s13018-024-05336-0","DOIUrl":"10.1186/s13018-024-05336-0","url":null,"abstract":"<p><p>Exosomes extracted from synovial fluid (SF-exo) reflect the status of their originating cells. The proteomic profiles of SF-exo are important for the diagnosis of osteoarthritis (OA). To delineate the proteomic differences between SF-exo from OA patients and healthy individuals, a quantitative proteomic study based on iTRAQ technology was performed. In this study, a total of 439 proteins were identified, with 20 proteins exhibiting increased expression in the OA patient group, while 5 showed decreased expression levels. Bioinformatic analysis showed these differentially expressed proteins (DEPs) were involved in a variety of immune-related processes, including complement activation and antigen binding. For further screening, we downloaded a publicly available dataset of synovial fluid (PXD023708) and compared it with our dataset. The comparative Results identified that 5 DEPs overlapped in two datasets, and protein-protein interaction revealed that C3, C4B and APOM were key members of a tightly interactive network. Through receiver operating characteristic (ROC) curve analysis and enzyme-linked immunosorbent assay (ELISA), we confirmed 5 DEPs (C3, C4B, APOM, MMP3, DPYSL2) as potential diagnostic biomarkers for OA. And Pearson correlation analysis confirmed that most of these biomarkers had no significant linear correlation with age. Overall, our study provides the first comprehensive description of the proteomic landscape of SF-exo in OA and identifies several potential biomarkers. These findings are expected to provide valuable insights into the diagnosis and treatment of OA.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"19 1","pages":"849"},"PeriodicalIF":2.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}