E. Saleh, Ahlam El-Boudy, Mohamed F. Gaber, Eman Ali
{"title":"Monitoring some pyrethroid pesticides residues in raw cow’s and buffalo’s milk","authors":"E. Saleh, Ahlam El-Boudy, Mohamed F. Gaber, Eman Ali","doi":"10.5455/DJVS.2021.197114","DOIUrl":"https://doi.org/10.5455/DJVS.2021.197114","url":null,"abstract":"Synthetic pyrethroid pesticides are widely used in animal husbandry and agriculture in Egypt. Excessive use of these pesticide lead to their accumulation in foods of animal’s origin and cause health problems. Therefore, this study was conducted to detect the concentrations of some synthetic pyrethroids by HPLC in raw cow and buffalo’s milk samples collected from different local markets and farms at Damanhour, El-Behira governorate, Egypt. The obtained results revealed that Flumethrin, Deltamethrin, Cyhalothrin and Cypermethrin were detected at incidence rate of 17.5 & 22.5; 25& 32.5; 15& 20 and 10 & 12.5% with a mean value of 36.14 ± 7.03 & 41.80 ± 7.91; 40.06 ± 8.71 & 52.70 ± 11.27; 24.65 ± 6.64 & 42.03 ± 8.25 and 89.27 ± 36.51& 121.58 ± 36.95 ppb, in cow and buffalo’s milk, respectively. α-cypermethrin could not be detected in any examined raw milk samples. Positive samples for synthetic pyrethroids residues compared with maximum residual limits established by international standards to detect samples below or above these limits. From the results in this study, farm milk was contaminated more than market’s milk which might be explained by presence of milk farms nearest to rural area where these synthetic pyrethroids are highly applied. This study would illustrate the risks of presence of synthetic pyrethroids residues in raw milk that threaten consumer health.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"134 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122704601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Clindamycin Oral Administration on Some Immunological and Biochemical Parameters of Newcastle Vaccinated Broilers","authors":"M. Eldakroury, AL-Haj Darwish, A. Darwish","doi":"10.21608/djvs.2021.91626.1045","DOIUrl":"https://doi.org/10.21608/djvs.2021.91626.1045","url":null,"abstract":"of Clindamycin on broiler vaccinated against Newcastle virus. Three groups of one day old Hubbard chicks each of 50 birds were used in this study. Group (1) was control non treated group (CG). Group C20 and C40 were given Clindamycin (20mg/kg and 40mg/kg body. wt) respectively in drinking water at 14th, 15th and 16th days of age. All groups were routinely vaccinated against Newcastle virus at 7 and 18 day of age. On the 25th day of age, blood samples were collected from ten birds of each group for immunological and biochemical tests. Five birds from each group were slaughtered at the same age then the lymphoid organs (thymus, bursa and spleen) were carefully separated & weighed and each organ relative weight was determined. Number of dead birds was recorded throughout the experiment period (45 day age). It was observed that administration of Clindamycin decreased the leukocytic count haemagglutination inhibition antibody titer (HI), total protein, globulin and the relative weight of bursa in C20 and C40. It did not induce any significant changes in the differential leukocytic count, liver and kidney function tests, spleen relative weight in C20 and C40. While, relative weight of thymus and body weight gain significantly decreased in C40 and non-significantly changed in C20. While, feed conversion rate significantly increased in C40 only. In addition, C20 and C40 had higher mortality rates than CG. Conclusion: Clindamycin administration suppresses the chicken's immune response to Newcastle disease virus vaccine.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114679228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gihan Eltanani, T. Gad, W. Darwish, W. El-Ghareeb, H. Ismail
{"title":"Prevalence of multidrug resistant Salmonella Typhimurium in retailed buffalo meat and offal with a reduction trial using rosemary and olive oils","authors":"Gihan Eltanani, T. Gad, W. Darwish, W. El-Ghareeb, H. Ismail","doi":"10.21608/djvs.2021.94666.1048","DOIUrl":"https://doi.org/10.21608/djvs.2021.94666.1048","url":null,"abstract":"rates of Salmonella spp. in the buffalo meat and edible offal (round, masseter muscles, liver, kidney, and trimmings) retailed in Zagazig city, Egypt. Second, serological identification of the isolated Salmonella spp., was followed. Third, screening of antimicrobial sensitivity testing of the identified Salmonella Typhimurium was done using the disk diffusion assay. Finally, the inhibitory effects of rosemary and olive oils against Salmonella Typhimurium were investigated. The obtained results in the present study revealed isolation of Salmonella spp., from the examined round, masseter muscles, liver, kidney, and trimmings at 15%, 25%, 35%, 25%, and 50%, respectively. Serological identification of Salmonella spp. revealed recovery of six serotypes namely, S. Typhimurium, S. Enteritidis, S. Kentucky, S. Inganda, S. Apeyeme, and S. Anatum from the examined samples at variable rates. The overall isolation rates of these serotypes were 26.64%, 29.97%, 16.65%, 9.99% 9.99%, and 6.66%, respectively. Salmonella Typhimurium isolates had clear multidrug resistance profiles. Rosemary and olive oils at 0.1%, and 0.5% could significantly reduce S. Typhimurium in an experimental trial in a concentration-dependent phenomenon.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130247785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asmaa Abd Elmacksood, O. Basha, D. Talat, H. Ahmed, M. S. Ibrahim
{"title":"In Vitro evaluation of antimicrobial activity of Lactobacillus acidophilus against some pathogens","authors":"Asmaa Abd Elmacksood, O. Basha, D. Talat, H. Ahmed, M. S. Ibrahim","doi":"10.5455/DJVS.2021.81934.1042","DOIUrl":"https://doi.org/10.5455/DJVS.2021.81934.1042","url":null,"abstract":"Bacterial infections cause severe losses in poultry farming. Clostridium perfringens and Escherichia- coli are two major pathogenic bacteria readily found in the broiler environment. They cause high- morbidity and mortality in poultry worldwide as a result of necrotic enteritis and colibacillosis, respectively. Furthermore, Staphylococcus aureus is an important cause of omphalitis, bumble foot and gangrenous dermatitis. Antimicrobial resistance is one of the most serious global public health threats that necessitates alternative strategies. Probiotics have been emerging as a safe and effective alternative to antibiotics. Lactobacillus being the most commonly used probiotic. Therefore, here the antimicrobial activity of Lactobacillus acidophilus against some pathogens namely Clostridium perfringens, Staphylococcus aureus and Escherichia coli were tested. The antibacterial effect of L. acidophilus was investigated by time kill assay, agar well diffusion and agar spot test. The obtained results showed that L. acidophilus had strong antimicrobial activity against the three bacterial pathogens. Moreover, L. acidophilus exhibited a strong autoaggregating phenotype and marked coaggregation with Cl. perfringens. In addition, inhibition of gas production from Cl. perfringens by L. acidophilus was evaluated. Alpha-toxin has been implicated as one of the major virulence factors of Cl. perfringens inducing avian necrotic enteritis. To investigate whether alpha toxin have adverse effects on L. acidophilus, viability assay proved that L. acidophilus was not remarkably affected by incubation with different concentrations of alpha toxin and different incubation time. Our results suggest that L. acidophilus exhibits strong inhibitory effects against Cl. perfringens, E. coli and S. aureus, and has strong co-aggregation abilities","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116799858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ibrahim, Ahmed Nomeir, Doaa Emara, A. E. El Sharaby
{"title":"Comparative pilot study of radiography and computed tomography for the thorax of Shirazi cat","authors":"A. Ibrahim, Ahmed Nomeir, Doaa Emara, A. E. El Sharaby","doi":"10.21608/djvs.2021.79115.1037","DOIUrl":"https://doi.org/10.21608/djvs.2021.79115.1037","url":null,"abstract":"The purpose of this pilot study was to validate the use of computed tomography (CT) for documenting the anatomy of the thorax of Shirazi cats. We used 3 clinically normal adult Shirazi cats, which were screened for radiographic and CT views and then used for reference gross dissection. Thorax CT scan displayed through hundreds of views the thoracic cage, trachea, bronchi, lungs, heart, vessels, mediastinum, and pleural cavity not only on sagittal and coronal planes but also – and exceptionally – on a transverse plane providing precise and unique information that was unobtainable with radiographs. CT scan also provided soft and lung windows, both of which enabled proficient tracing of the position, shape, and size of all the thoracic structures. The obtained radiographic and CT findings in the thorax of the investigated cats have been compared, and several anticipated parameters of the two screening techniques were evaluated and discussed. Our results indicated that radiographic scans remain a fast and easy start point for thoracic examination enough for an emergency diagnosis, whereas CT images are superior to identify anatomical structures and subsequent clinical interpretation of the feline thoracic affections. Anatomical reference study of the thoracic CT images is proposed for all screening levels of Shirazi cat.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127130511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular characterization of listeria monocytogenes isolated from raw milk and some dairy products at local markets in Damanhour city, Egypt","authors":"E. Saleh, Ahlam Elboudy, A. Elsayed, Eman Ali","doi":"10.5455/DJVS.2021.187849","DOIUrl":"https://doi.org/10.5455/DJVS.2021.187849","url":null,"abstract":"Several outbreaks of L. monocytogenes have been involved with milk and dairy productsconsumption. The present study was conducted to investigate the prevalence listeria monocytogenes in raw milk and some dairy products. A total of 225 samples of raw milk (75), pasteurized milk (50), ice cream (50) and Ras cheese (50) were collected randomly from different supermarkets, retail outlets and other markets outlets in Damanhour city, El-Behira governorate, Egypt. Out of 225 samples, 29 (12.88%) were positive for Listeria species. The occurrence of Listeria spp. in raw milk, pasteurized milk, ice cream and Ras cheese samples were 10 (13.33%), 6 (12%), 7 (14%) and 6 (12%), respectively. The most prevalent listeria species isolated from raw milk and dairy products in this study was L. innocua and L. monocytogenes. The biochemically identified isolates of L. monocytogenes (16) were molecularly characterized by multiplex PCR for detection of three virulence genes (iap , hylA and actA ); the results showed that iap gene was demonstrated in all isolates (100%); hylA and actA were detected in 83.3 and 66.7% of isolates from raw milk; 66.7 and 66.7% of isolates from pasteurized milk; 80 and 80% of isolates from ice cream; 100 and 50% of isolated from Ras cheese samples. Concerning to antibiotic resistance, 16 isolates of L. monocytogenes were tested against 14 antibiotics disc and showed that all the isolates were resistant to Kanamycin (100%) and Nalidixic acid (93.75%), meanwhile, most of the isolates showed sensitivity against Ciprofloxacin (87.50%) and Ampicillin (68.75%). In conclusion, the results of this study emphasize the need for applying more strict hygienic control measures especially during processing, storage and marketing of dairy products.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123774805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mousa A. Ayoub, Mohammad A. Nossair, A. Eid, Sabah I Shaaban
{"title":"A survey of some zoonotic diseases in cattle slaughtered at local abattoir, Behera Governorate","authors":"Mousa A. Ayoub, Mohammad A. Nossair, A. Eid, Sabah I Shaaban","doi":"10.5455/DJVS.2020.52679.1028","DOIUrl":"https://doi.org/10.5455/DJVS.2020.52679.1028","url":null,"abstract":"The current study was carried out to estimate the prevalence of some zoonotic diseases transmitted through meat including; cysticercosis, tuberculosis and brucellosis in slaughtered bovine stock (aged >2 years) at Abo El Marameer abattoir, Behera, Egypt. Prevalence estimation of the three zoonotic diseases was undertaken through an active abattoir and sero-survey was carried out during the period of January 2018 and December 2019. A total of 1200 Serum samples collected from the slaughter stock were serologically screened for antibodies against brucellosis using Rose Bengal plate test. The same animals were examined for presence of gross lesions of tuberculosis and cysticercosis. Post mortem examination of slaughtered cattle showed a prevalence of, 1.5% (18) for cysticercosis and 1.25% (15) for tuberculosis. In these two zoonoses, a statistically significant difference in infection rates was noted between different breeds. In addition, the overall seroprevalence of animals with brucellosis antibodies were found to be 7.3% (88). The study findings suggested that brucellosis, tuberculosis and cysticercosis were prevalent in the study area and provide definitive evidence of slaughtered stock exposure to these zoonotic agents with concurrent public health consequences.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"282 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122950399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prevalence of Listeriosis in some farm animals","authors":"H. Farag, M. Abdallah, Mohammad A. Nossair","doi":"10.5455/DJVS.2021.69592.1034","DOIUrl":"https://doi.org/10.5455/DJVS.2021.69592.1034","url":null,"abstract":"The study was carried out in animal farms located in North Coast and Desert Road, Egypt. A total of 175 faecal samples were collected from different species of farm animals for investigating the incidence of Listeria species. Samples were collected from private farms and small holders of dairy animals, including cattle (70), buffaloes (30), sheep (50) and goats (25). Results revealed 17.1% total incidence of Listeria species, where the incidence rate within the same animal species was higher among cattle (18.6%) followed by sheep (18.0%), buffaloes (16.7%) and goats (12.0%) at last. Moreover, the most recovered species was L. ivanovii (6.3%) followed by L. monocytogenes and L. grayi (4% for each of them), then L. innocua (2.9%). Results also showed that L. ivanovii and L. grayi were the most recovered species from cattle (5.7% for each) and buffaloes (6.7% for each), while the highest isolated species from sheep and goats were L. monocytogenes (8%) and L. ivanovii (8%), respectively. On the other side, L. monocytogenes could not recover from buffaloes and goats. Presence of Listeria species specially L. monocytogenes and L. ivanovii in faeces of farm animals attracts the attention to the way in which these wastes must be treated and dealt with in order to avoid contamination of milk and its further products that finally can carry the infection to man.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"184 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114735984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. E. Alsenosy, H. Ghoneim, Safinaz S. Ibrahim, Asmaa Aboelnour
{"title":"Expression of Matrix Metalloproteinase 9 in Oreochromis Niloticus Fish and Its Active Role in Initiation and Resolution of Ammonia","authors":"A. E. Alsenosy, H. Ghoneim, Safinaz S. Ibrahim, Asmaa Aboelnour","doi":"10.21608/djvs.2020.53743.1031","DOIUrl":"https://doi.org/10.21608/djvs.2020.53743.1031","url":null,"abstract":"Dietary protein for reaching better growing Oreochromis niloticus including gradual protein levels, taking into account that optimum protein levels for grown fish will help to reduce the impact of ammonia on fish health. Herein, this study verified a theory that assumes ammonia induces changes in fish tissues responsible for ammonia excretion by a mechanism coupled to capillary endothelial cells. Fish feeding on a higher level of protein caused the expression of MMP markers. Alongside, ammonia raised the activity of interstitial matrix metalloproteinases MMP-9. The rise of cell permeability may be amended by using an inhibitor of MMP. The results support the idea that stimulation of MMPs in brain endothelial cells contribute to the variations in permeability and cerebral vasogenic component, renal and gill tissues associated with ammonia implication. Detailed considerate of the response to ammonia stress and tolerance in tilapia can provide useful information for fish breeding professionals to expand aquaculture.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127010342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of some organic acids on microbial quality of dressed cattle carcasses in Damietta abattoirs, Egypt.","authors":"E. Saleh, F. Shaltout, Essam Abd Elaal","doi":"10.5455/DJVS.2021.186008","DOIUrl":"https://doi.org/10.5455/DJVS.2021.186008","url":null,"abstract":"This experimental study aimed to investigate the anti-microbial effect of some organic acids (OA) represented by Acetic and Lactic acids of (1 and 2%), and assess its reflection on the microbiological quality of dressed cattle carcasses slaughtered in Damietta city abattoirs. Samples were grouped according the concentration of the used acid to five groups, where each group consisted of five carcasses. Acids were applied as nozzle sprays over the external surface of the carcasses and kept for 20 minutes before swab sampling. Swabs were examined for aerobic plate count (APC), Enterobacteriacae count (EC), Coliform count (CC), Staphylococcus count (SC), mould and yeast counts before and after spraying. Results revealed significant reductions of the assessed microbial counts in both lactic and acetic acids of both concentrations, except fungal counts which revealed insignificant reductions for both acids. Moreover, Gram negative bacteria (Enterobacteriacae) which showed greater sensitivity to the used organic acids than Gram positive bacteria (Staphylococcus), where greater concentration gave greater reduction in the bacterial counts. Moreover, spray wash of lactic acid resulted in higher reduction of bacterial counts on meat surface than acetic acid. From the obtained results, organic acids showed safe, simple, efficient, cheap, and highly effective modality of meat decontamination, on addition, application of lactic acid 2.0% spray showed higher anti-bacterial effect, therefore, it is recommended to improve safety of sheep carcasses for industrial scales.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123885557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}