Damanhour Journal of Veterinary Sciences最新文献

{"title":"Genetic point mutation inducing antigenic drift in hypervariable region of a very virulent IBDV isolate in chickens in Egypt during 2014-2016","authors":"H. Ellakany, A. Elbestawy, A. Sayed-Ahmed, S. Elgammal, A. Gado, Hatem Abdel-Hameed","doi":"10.5455/DJVS.2019.49553","DOIUrl":"https://doi.org/10.5455/DJVS.2019.49553","url":null,"abstract":"Infectious bursal disease (IBD) is a highly contagious viral disease affecting young chickens causing immune suppression, high morbidity and mortality. Its economic significance is recognized worldwide. In this study, suspected IBD samples (bursa of Fabricious) were collected from 45 chicken flocks in 3 Egyptian governorates from 2014 to 2016. The virus was inoculated in embryonated chicken eggs via chorio-allantoic- membrane (CAM) route inducing specific IBDV lesions in the embryos. Viral identification was carried out through Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) targeting VP2 gene. Fourteen positive IBDV isolates (31%) were confirmed by RT-PCR. Three pure IBDV isolates were subjected to partial VP2 gene sequence analysis from which 2 IBDV isolates No. 1 and 3 (Accession No. KX827589.1 and MK906027) were defined as a very virulent IBDV (vvIBDV) genogroup 3, while the third isolate (Accession No. KX827588.1) was closely related to a vaccine strain in cvIBDV genogroup 1. Nucleotide and amino acid sequence analysis and blast of the IBDV isolates indicated a close relationship with the previously recorded Egyptian IBDVs with 96 to 99% identity. Point mutation or amino acid substitution in positions P202M (conserved region); and A211T, D212Y (hypervariable region) of the VP2 gene in the isolate No. 3 vvIBDV (Accession No. MK906027) that differ from all the previously recorded Egyptian isolates in GenBank were present.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126849578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Analysis of Highly Pathogenic Avian Influenza H5N1 in West Delta Governorate","authors":"S. Haggag, H. S. Abd-Elhamid, A. Arafa, A. Elbestawy, H. Ellakany, Ahmed Salah eldin, A. Gado","doi":"10.5455/djvs.46042","DOIUrl":"https://doi.org/10.5455/djvs.46042","url":null,"abstract":"Avian influenza viruses became widely distributed in most Middle Eastern countries, causing high mortality rate and severe economic losses in poultry industry especially when complicated with other pathogens. The continuous evolution of highly pathogenic H5N1 avian influenza viruses (HPAI- H5N1) has resulted in multiple diverse groups based on their haemagglutinin (HA) sequences. In this study, samples from 40 broiler chicken farms during 2014 - 2017 were screened for HPAI- H5N1 Results showed that 15 flocks (37.5%) were positive for HPAI-H5 all of them non vaccinated . Furthermore, full sequencing of HA and NA gene for 3 isolates isolated during 2017 showed that the viruses were clustered with Egyptian viruses from clade 2.2.1.2 and closely related to viruses from neighboring countries.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114640170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of thymol and carvacol oils supplementation on meat quality of broiler chickens","authors":"E. Saleh, Mahmoud Seddeik, Reem Abdelwahab","doi":"10.21608/djvs.2019.49552","DOIUrl":"https://doi.org/10.21608/djvs.2019.49552","url":null,"abstract":"","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114970445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth potential and body composition of the African catfish Chrysichthys nigrodigitatus (Lacépède, 1803) juveniles fed different locally available feed ingredients","authors":"Gboko K.C, Koumi A.R., Atse B.C., T. K.","doi":"10.21608/djvs.28287","DOIUrl":"https://doi.org/10.21608/djvs.28287","url":null,"abstract":"","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"18 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114020458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raw Milk as a Vehicle of Brucella Infection for Human in Behera Province","authors":"Mousa A. Ayoub, A. Badran, Mohammad A. Nossair","doi":"10.5455/djvs.32476","DOIUrl":"https://doi.org/10.5455/djvs.32476","url":null,"abstract":"A B S T R A C T Brucellosis is a worldwide zoonosis that has major public health concern in Egypt. The present work was conducted to investigate the seroprevalence of brucellosis in milk as well as in serum samples of humans on basis of the Milk Ring Test (MRT) and Rose Bengal plate test (RBPT), respectively and further confirmation by polymerase chain reaction (PCR). A total of 240 raw milk samples (98 from dairy markets, 128 samples from farm with history of mastitis and 14 samples from retailers) were collected during the period extended from November, 2017 to October, 2018 beside another 100 serum samples were collected from patients attending fever hospitals (60), individuals with occupational contact with investigated animals (20) and apparently healthy individuals attending private laboratories in Behera seeking for medical advice (20). It was found that the overall prevalence of brucellosis in milk was 10.8% by MRT. Also, it was observed that sero-prevalence of Brucella Abs in milk samples in relation to sources of milk revealed that the highest rate was recorded in milk samples collected from retailers (14.3%) followed by those collected from dairy farms (12.5%) then those of dairy markets (8.2%) with non-significant association between the rates of detection. Concerning season, there was highly significant relationship between season and percent of infection with Brucella, the high percent of infection found in winter season (15.4%) than in summer season (6.5%). On the other side, the seroprevalence of Brucella Abs in human serum samples by RBPT clarified that the highest detection rate occurred in individuals with occupational contact (25%) followed by patients with fever complain (18.3%) and finally apparently healthy individuals (10%) with significant association between them. Seasonal prevalence in human samples clarified higher summer prevalence (20.5%) compared to winter (16.4%). Moreover, there was significant relationship between age and infection rate in human (P<0.05), with higher percentage of infection recorded in age group over than 40 years by 23.3% (10/43) followed by age group (15-40 years) (16.7%) (6/36) and lastly those less than 15 years (9.5%) (2/21). From our result, it is concluded that MRT and RBPT used as screening tests for detection the prevalence of species in milk and serum samples, Brucella infection is found with high percent in milk in Behera Province highlighting the hazardous role in milk in transmission of Brucella infection to consumers specially those used to drink raw milk in rural areas. In addition PCR is gold confirmatory technique for diagnosis of brucellosis in both milk and blood. .","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131405844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of melatonin in preventing thioacetamide-induced liver injury in rats","authors":"S. Abdou, Nabil M. Taha, M. Lebda, H. Elhoffy, A. Hashem","doi":"10.21608/djvs.42702","DOIUrl":"https://doi.org/10.21608/djvs.42702","url":null,"abstract":"","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128892451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt","authors":"Walaa Ibrahim","doi":"10.21608/djvs.38982","DOIUrl":"https://doi.org/10.21608/djvs.38982","url":null,"abstract":"","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"330 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133410506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical alterations associated with experimentally induced breast cancer in rats","authors":"Mohamed M. Zeweil, K. Sadek","doi":"10.5455/DJVS.32001","DOIUrl":"https://doi.org/10.5455/DJVS.32001","url":null,"abstract":"This study was conducted to demonstrate the preventive graviola effect on breast cancer induced by 7,12-dimethylbenz [a] anthracene (DMBA) in fifty female rats distributed into four groups. Group I: Control group injected orally by physiological saline, group II: DMBA induced-breast cancer, injected orally a single dose of DMBA (50mg/kg) diluted in sesame oil (1 ml), group III: Graviola 200 mg/kg two times per week given orally by gavage from the first day of the experiment till the end plus a single dose of DMBA (50mg/kg) diluted in sesame oil (1mL) given orally at age of 57 days and group IV: rats treated with single dose of DMBA (50mg/kg) diluted in sesame oil (1mL) given orally plus graviola 200 mg/kg two times weekly both at age of 57 days till the end. After 30w the animals were anaesthetized to collect blood samples to determine the hepatic and renal protection of graviola. Treatment with graviola significantly (p< 0.05) reduced ALT activity and creatinine level. We can conclude that graviola mitigated hepatic and kidney functions.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134421206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in growth and fat deposition between White Plymouth Rock and Nagoya breeds as a tool for QTL analysis","authors":"I. a, Mohammed Eledel, B. Essa","doi":"10.21608/djvs.26853","DOIUrl":"https://doi.org/10.21608/djvs.26853","url":null,"abstract":"Two breeds of chickens were used in this study White Plymouth Rock (WPR ( and Nagoya (NAG) in addition to tracking of their crossbred (♀ WPR Χ ♂ NAG) of two generations (F1and F2) for evaluating the differences in growth characteristics and fat deposition. Body weights were measured weekly from the first day (hatch day) till 4 weeks of age and different weight gains were also calculated. In addition to feed intake (for the period of 3-4 weeks of age) and feed conversion ratio were estimated. At four weeks of age, birds were dissected and the major internal organs including liver and gizzard were weighed, moreover the parts of breast muscle were also recorded. The results showed that, WPR was significantly higher in body weights, body weight gains and feed intake than NAG chicken. Also, WPR showed significantly larger sizes for liver, gizzard, pectoral muscles, abdominal fat (AF), ventriculus fat (VF), subcutaneous fat (SF) and total fat weight than NAG chicken. The correlation analysis showed that body weight at one week of age and body weight gain of the first week of age were found to have positive correlation with liver triglycerides and serum triglycerides levels in WPR while it was associated with decreasing of level of total cholesterol in serum in NAG. These results served as evidence for elucidating the major differences observed between the two breeds concerning growth and fat deposition. In addition to the relationship between increasing body weight and fat deposition was established. Thus, future QTL analysis can be performed for identification of chromosomal regions controlling growth and fatness traits and subsequently candidate gene influencing these traits could be revealed in further investigations.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"327 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133127731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the protection of commercial live and inactivated NDV vaccines against Newcastle virus genotype VIId circulating in the field","authors":"H. Ellakany, H. S. A. El-Hamid, S. Nasef, S. Nasr, M. A. Aziz, A. Gado, Rasha Zedan","doi":"10.5455/DJVS.25271","DOIUrl":"https://doi.org/10.5455/DJVS.25271","url":null,"abstract":"The most prevalently circulating genotype of Newcastle disease (NDV)in Egypt was VII of class II NDV strains. Control of NDV by vaccinationis a common strategy in intensively raised commercial flocks andNewcastle disease vaccines have been used worldwide to protect againstclinical signs and mortalities. The aim of the present study was to evaluatethe level of protection against clinical disease and virus shedding affordedby different NDV vaccines. The strain used for challenge wasNDV/CH/EG/18/2015 (GenBank accession number- KU377781),classified as Genotype VIId with ICPI of 1.89, and MDT of 48 hrs. Broilerchickens were challenged intra-occularily at 28 days of age using 100 μl of106 EID50 per dose/bird. Results indicated that protection percent againstmortality and clinical signs was 100 % and 93.3 % for inactivated NDVvaccine and LaSota, respectively. Shedding was not prevented by anyvaccine.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122891385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}