Journal of Ovarian Research最新文献

{"title":"The role of Chinese herbal medicine in diminished ovarian reserve management.","authors":"Xiaoyu Zhang, Na Zhang, Zhibin Dong, Hao Sun, Zhihao Diao, Yujie Li, Dongqing Du, Yuxia Ma","doi":"10.1186/s13048-025-01669-4","DOIUrl":"https://doi.org/10.1186/s13048-025-01669-4","url":null,"abstract":"<p><p>Diminished Ovarian Reserve (DOR) is characterized by a reduction in the number of available follicles in the ovaries, leading to hormonal imbalances, decreased ovarian reserve, and reduced fertility. Clinically, it presents with elevated follicle-stimulating hormone (FSH) levels, decreased anti-Müllerian hormone (AMH) levels, and a lower antral follicle count (AFC). In recent years, Traditional Chinese Medicine (TCM) has gained recognition for its multi-target, holistic regulation in treating DOR, offering broad therapeutic effects with minimal side effects. This review aims to summarize the mechanisms and clinical efficacy of Chinese herbal medicine (CHM) formulas and active compounds in the treatment of DOR, providing theoretical support for their clinical application and future research. A systematic literature search was conducted from June 2019 to June 2024, and 12 clinical studies along with 38 basic research papers were selected. The findings suggest that CHM formulas primarily act by counteracting oxidative stress, regulating immune defense, modulating sex hormone secretion via the hypothalamic-pituitary-ovarian axis, and inhibiting excessive apoptosis of ovarian granulosa cells. This review highlights the therapeutic potential of TCM for improving ovarian function, regulating endocrine balance, and alleviating DOR symptoms, offering valuable insights for clinical practice and research.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"90"},"PeriodicalIF":3.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of inflammatory blood parameters from complete blood count in predicting ovarian follicular density in cancer patients undergoing ovarian tissue cryopreservation.","authors":"Livia Ronchetti, Irene Terrenato, Anna Maria Lobascio, Marcello Iacobelli, Margherita Ferretti, Nouha Setti Boubaker, Francesca Sperati, Chiara Mandoj, Emanuela Mancini, Mariantonia Carosi, Enrico Vizza, Giacomo Corrado","doi":"10.1186/s13048-025-01670-x","DOIUrl":"https://doi.org/10.1186/s13048-025-01670-x","url":null,"abstract":"<p><strong>Background: </strong>Inflammation is a key feature of neoplastic diseases, especially cancer. Predicting follicular density (FD) in ovarian cortical tissue is essential for evaluating ovarian tissue cryopreservation (OTC) outcomes in fertility preservation. However, to date, no studies have explored the role of inflammatory markers in predicting FD in OTC patients. This study aims to investigate the relationship between blood inflammatory parameters and FD in this population.</p><p><strong>Methods: </strong>We conducted a retrospective observational study on 101 OTC patients. The primary goal was to assess whether parameters from Complete Blood Count (CBC) that include White Blood Cells (WBC), absolute neutrophil count, absolute lymphocyte count, Neutrophil/Lymphocyte Ratio (NLR), Mean Platelet Volume (MPV), Platelet Count (PC), MPV/PC and the Platelet/Lymphocyte Ratio (PLR) could predict FD. We also evaluated the impact of factors such as oncological diagnosis, smoking, Body Mass Index (BMI), and germline BRCA mutations. Spearman's correlation coefficient and the Mann-Whitney test were used for analysis.</p><p><strong>Results: </strong>Significant correlations were found in patients aged between 27 and 31. In this group, NLR was inversely correlated with FD (Rho = -0.374, p = 0.032), while lymphocyte count (Rho = 0.371, p = 0.034) and MPV/PC (Rho = 0.365, p = 0.037) were positively correlated with FD. An inverse correlation was also found between PLR and FD (Rho = -0.38, p = 0.028).</p><p><strong>Conclusions: </strong>Our findings suggest that NLR, lymphocyte count, MPV/PC and PLR may be useful in predicting FD in a subgroup of OTC patients. Larger studies are needed to confirm these results.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"89"},"PeriodicalIF":3.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-transcriptome sequencing reveals the effects of acupuncture on early embryos post-IVF-ET in poor ovarian response.","authors":"Jianheng Hao, Yuemeng Zhao, Xuan Liu, Huichao Xu, Liying Liu, Haijun Wang, Ying Lan, Laixi Ji","doi":"10.1186/s13048-025-01682-7","DOIUrl":"https://doi.org/10.1186/s13048-025-01682-7","url":null,"abstract":"<p><strong>Background: </strong>Poor ovarian response (POR) refers to a pathological condition where the ovaries respond inadequately to gonadotropin stimulation during ovulation induction. The decline in both the quantity and quality of available oocytes makes achieving pregnancy through IVF-ET more challenging. Several studies have demonstrated that acupuncture can improve embryo quality, increase the number of high-quality embryos, and enhance pregnancy outcomes in IVF-ET patients, although the underlying mechanisms remain unclear. Therefore, this study aimed to investigate the molecular mechanisms by which acupuncture influences early embryo development in POR mice after IVF-ET through whole-transcriptome sequencing.</p><p><strong>Methods: </strong>We established POR mice model and performed acupuncture treatment. Fresh denuded oocytes were retrieved and transplanted via IVF-ET into new donor female mice to obtain early-stage embryo tissues. Vaginal smear tests were conducted to monitor estrous cycle changes, while oocyte retrieval, ovarian wet weight, and ovarian index were assessed. Serum concentrations of anti-Müllerian hormone (AMH), follicle-stimulating hormone (FSH), estradiol (E2), and luteinizing hormone (LH) were measured via ELISA. Histopathological changes and apoptosis in the ovaries were evaluated using HE and TUNEL staining. Whole-transcriptome sequencing was employed to establish expression profiles of differentially expressed mRNAs (DEmRNAs), DEmiRNAs, DElncRNAs, and DEcircRNAs. The circ/lncRNA-miRNA-mRNA network was constructed to analyze the biological functions and potential mechanisms of acupuncture on early embryo development post-IVF-ET in POR mice.</p><p><strong>Results: </strong>Our results demonstrated that acupuncture improved ovarian function in POR mice, corrected serum hormone imbalances, and alleviated abnormal apoptosis in ovarian granulosa cells. Through whole-transcriptome sequencing, we identified 685 DEmRNAs, 13 DEmiRNAs, 325 DElncRNAs, and 4 DEcircRNAs exhibiting regulatory trends. By constructing the circ/lncRNA-miRNA-mRNA network, we found that miR-291a-3p, miR-294-3p, and miR-295-3p may serve as key targets influencing early embryo development via multiple pathways, including the Toll-like receptor signaling pathway and p53 signaling pathway.</p><p><strong>Conclusion: </strong>Overall, our study is the first to reveal the molecular mechanisms by which acupuncture improves early embryo development under POR conditions, providing new evidence for the use of acupuncture in assisted reproduction.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"91"},"PeriodicalIF":3.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of preoperative immunonutritional status on postoperative complications in ovarian cancer.","authors":"Xingyu Liu, Ming Li, Yingjun Zhao, Xiaofei Jiao, Yang Yu, Ruyuan Li, Shaoqing Zeng, Jianhua Chi, Guanchen Ma, Yabing Huo, Zikun Peng, Jiahao Liu, Qi Zhou, Dongling Zou, Li Wang, Qingshui Li, Jing Wang, Shuzhong Yao, Youguo Chen, Ding Ma, Ting Hu, Qinglei Gao","doi":"10.1186/s13048-025-01624-3","DOIUrl":"https://doi.org/10.1186/s13048-025-01624-3","url":null,"abstract":"<p><strong>Background: </strong>Preoperative immunonutritional status can influence postoperative complications. Malnutrition in ovarian cancer patients diminishes the body's resilience to abdominal surgery, resulting in inferior surgical outcomes and increased postoperative complications. We aim to investigate the effect of preoperative immunonutritional status, including NLR, PLR, LMR, TCLR, FAR, FLR, SII, PNI and CONUT on postoperative complications in epithelial ovarian cancer (EOC) in a large population.</p><p><strong>Methods: </strong>A multicenter real-world study included 922 patients with histologically confirmed EOC who received comprehensive staged surgery or debulking surgery at seven tertiary hospitals in China between 2012 and 2023. Logistic regression and Lasso regression analyses were employed to identify variables associated with postoperative complications. A predictive nomogram model was developed based on multivariate modeling.</p><p><strong>Results: </strong>The study included a total of 922 patients diagnosed with epithelial ovarian cancer across seven medical centers with 565 (61.3%) patients experiencing postoperative complications. Significant differences were found in the distribution of inflammatory and nutritional risk indicators, including NLR, PLR, LMR, TCLR, FAR, FLR, SII, PNI and CONUT between the two groups (all P < 0.01). A multivariable model identified several predictive factors for postoperative complications: PNI > 46.73 (odds ratio [OR] = 0.49, P < 0.001), FAR > 10.77 (OR = 1.60, P = 0.019), LMR > 3.70 (OR = 0.68, P = 0.044), hydrothorax (OR = 2.60, P = 0.005), laparoscopy (OR = 0.59, P = 0.010 vs. laparotomy), enterectomy (OR = 2.50, P = 0.001).</p><p><strong>Conclusion: </strong>Poor immunonutritional status can increase the risk of postoperative complications. These findings suggest that prompt nutritional interventions may reduce the incidence of postoperative complications and improve surgical outcomes. The risk prediction model, including PNI, FAR, LMR, hydrothorax, laparoscopy vs. laparotomy, and enterectomy, might facilitate patient-centered decision-making and risk stratification.</p><p><strong>Clinical trial registration: </strong>The study was registered in the Clinical trial registry: NCT06483399. ( https://clinicaltrials.gov/study/NCT06483399 ).</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"88"},"PeriodicalIF":3.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tanshinone IIA alleviates tri-ortho-cresyl phosphate-induced ovarian damage through Hippo signaling pathway activation in mice.","authors":"Zhangqiang Ma, Na Hu, Liping Zheng, Yue Xue, Chong Zhou, Wencan Wang, Xiu Cheng, Tao Luo, Jianlin Yu, Liaoliao Hu","doi":"10.1186/s13048-025-01671-w","DOIUrl":"https://doi.org/10.1186/s13048-025-01671-w","url":null,"abstract":"<p><strong>Background: </strong>Tri-ortho-cresyl phosphate (TOCP), a widely used plasticizer, has been shown to impair ovarian function. While tanshinone IIA exhibits ovarian protective effects in aging models, its potential to counteract TOCP-induced ovarian damage and associated signaling mechanisms remains unexplored. This study investigates the therapeutic effects of tanshinone IIA on TOCP-damaged ovaries in mice, with focus on Hippo, AKT, and MAPK pathways.</p><p><strong>Results: </strong>TOCP exposure (200 mg/kg/d for 28 days) significantly reduced ovarian follicle counts (primordial, preovulatory, and mature follicles) and disrupted hormone levels (elevated Estrogen(E2), decreased Follicle stimulating hormone(FSH)/ Anti-Mueller tube hormone(AMH)) in mice. Treatment with high-dose tanshinone IIA restored ovarian structure and function: growing follicle counts increased significantly (p < 0.001), FSH (p < 0.001) and AMH (p < 0.001) levels surged to marked degrees, while E2 (p < 0.001) levels decreased significantly. All changes were statistically significant. Immunohistochemistry and Western blot analysis revealed that tanshinone IIA restored ovarian AMH and Follicle-Stimulating Hormone Receptor (FSHR) protein expression, which were suppressed by TOCP. In vitro experiments further demonstrated that TOCP dose-dependently inhibited granulosa cell viability (p < 0.001) and proliferation (p < 0.001). Co-treatment with tanshinone IIA (0.01 mM) rescued cell viability (p < 0.01) and proliferation (p < 0.05). Mechanistically, tanshinone IIA suppressed ovarian apoptosis (p < 0.01) and modulated multiple signaling pathways: it attenuated Hippo signaling (p < 0.05) and reactivated PI3K/AKT (p < 0.05), p38 (p < 0.05), and ERK1/2 (p < 0.01) pathways.</p><p><strong>Conclusions: </strong>Tanshinone IIA alleviates TOCP-induced ovarian dysfunction primarily through coordinated modulation of Hippo signaling and AKT/MAPK pathway activities, offering a potential therapeutic strategy for chemical-induced ovarian injury.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"85"},"PeriodicalIF":3.8,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12032754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yijing Decoction improves premature ovarian failure in rats by activating VEGF/VEGFR-2/FAK pathway.","authors":"Ranran Gao, Yeke Wu, Yuqin Tang, Keming Wu, Baojun Guo","doi":"10.1186/s13048-025-01679-2","DOIUrl":"https://doi.org/10.1186/s13048-025-01679-2","url":null,"abstract":"<p><strong>Background: </strong>Premature ovarian failure (POF) is defined as amenorrhea that occurs before the age of 40 when the ovaries weaken or even fail. This disease seriously affects a woman's future health and fertility.</p><p><strong>Methods: </strong>Potential targets of Yijing Decoction (YJD) and POF were predicted by web-based pharmacology-related databases. The POF rat models and human ovarian granulosa cells injury models were induced by triptolide. In addition, the estrous cycle of the rats was monitored by vaginal smear and the ovarian tissue morphology was stained by HE staining. Immunohistochemistry, qRT-PCR and Western blotting were used to evaluate the levels of reproductive and angiogenesis related factors. Moreover, serum levels of the sex hormones and the oxidative stress indicators were measured by ELISA.</p><p><strong>Results: </strong>YJD treatment resulted in the improvement of triptolide-induced abnormal ovarian function by restoring normal estrous cycle, maintaining nearly normal ovarian size, reducing follicular atresia and increasing vascularization. Additionally, YJD treatment normalized the serum levels of P, E2, FSH, LH, AMH, MDA and SOD, while activating the VEGF/VEGFR-2/FAK pathway. However, the VEGF/VEGFR-2/FAK pathway inhibitors reversed these pharmacological effects that YJD exhibited in POF rats. Furthermore, YJD increased the pregnancy rate and the number of live births in POF rats.</p><p><strong>Conclusion: </strong>YJD reduced oxidative stress level, promoted angiogenesis and improved ovarian function in POF rats by activating VEGF/VEGFR-2/FAK pathway. Moreover, YJD improved the intrauterine microenvironment for implantation in POF rats, thereby improving fertility.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"87"},"PeriodicalIF":3.8,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12034217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A compound heterozygous mutation in ZP1 and two novel heterozygous cis mutations in ZP3 causes infertility in women presenting with empty follicle syndrome.","authors":"Xiaoxiao Wang, Yingxue Liu, Guanghui Yuan, Jingjie Yang, Xiaowen Liu, Shuyuan Chen, Huaiqian Dou, Panpan Lu, Linfang Han, Duan Li, Cuifang Hao","doi":"10.1186/s13048-025-01674-7","DOIUrl":"https://doi.org/10.1186/s13048-025-01674-7","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Infertility, as a major human reproductive health problem, affects approximately 17.5% of the global population. The emergence of assisted reproductive technology, particularly in vitro fertilization (IVF), has resolved the reproductive challenges of most infertile couples. In IVF, there exists a group of women who are unable to retrieve oocytes from typically developing follicles despite a positive response to ovarian stimulation, a condition clinically referred to as empty follicle syndrome (EFS). The pathogenesis of EFS is still unclear, but increasing evidence suggests that genetic factors play a very significant role. A clinical and experimental analysis of two pedigrees was performed in this study to investigate the genetic basis of EFS.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Genomic DNA was extracted from peripheral blood samples for whole-exome sequencing in EFS patients. The identified variants were validated by Sanger sequencing. Computer simulation of protein structure was used to assess the pathogenicity of the variants on the proteins. The effects of the ZP1 and ZP3 variants on protein expression were probed via western blotting, and the effects of the variants on protein localization were probed by immunofluorescence. ELISA and Co-IP were used to detect the effects of variants on protein secretion and interactions.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In this study, we identified a compound heterozygous mutation in ZP1 (c.[2T &gt; A]; [1429G &gt; T]) and two novel ZP3 heterozygous cis mutations (c.[724G &gt; T;815 A &gt; G]) from two EFS patients, respectively. These mutations are highly conserved between different species. Through in vitro experiments, we showed that the ZP1 (p.[Met1?]; [Gly477*]) mutations result in reduced protein expression, whereas the ZP3 (p.[Asp242Tyr; Asn272Ser]) mutations lead to increased protein expression. However, neither mutation affected the subcellular localization of the ZP proteins. Bioinformatic analysis revealed that these mutations disrupt the conformation of the ZP protein, which may affect its stability and binding capability. Functional experiments showed that the ZP1 and ZP3 mutations altered the interaction between themselves and ZP2 proteins; the ZP1 mutation inhibited ZP1 protein secretion, whereas the ZP3 mutation increased the secretion of ZP3 protein, which may affect ZP assembly.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Our study has enriched the mutational spectrum of the ZP gene by identifying mutations in the causative genes ZP1 and ZP3 associated with EFS. In vitro experiments exploring the effects of mutations on ZP protein expression and function confirmed that ZP is an important genetic cause of EFS, thus broadening our understanding of the genetics of female infertility. We emphasize the importance of genetic analysis in the diagnosis and prognosis of \"genuine\" EFS (GEFS) and recommend that EFS patients strive for a successful pregnancy through an oocyte donation program.&lt;/","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"86"},"PeriodicalIF":3.8,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12032726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-rich plasma activation: are there differential effects in reproductive medicine?","authors":"Eduardo Anitua, María de la Fuente, Roberto Prado, Mohammad H Alkhraisat","doi":"10.1186/s13048-025-01667-6","DOIUrl":"https://doi.org/10.1186/s13048-025-01667-6","url":null,"abstract":"<p><p>The aim of this in vitro short report was to determine whether activation of platelet-rich plasma (PRP) results in differential effects on growth factor release and cell proliferation in reproductive medicine. This study involved PRP from five donors, activated with either CaCl₂ (exogenous activation) or type I collagen (mimicking endogenous activation). The release of growth factors (EGF and PDGF-AB) and the proliferative response of human ovarian fibroblasts were analysed. PRP activated with CaCl₂ formed stable clots and released statistically significant higher levels of EGF (871 ± 426 pg/mL) and PDGF-AB (26535 ± 6477 pg/mL) compared to collagen-activated PRP (EGF: 141 ± 66 pg/mL, PDGF-AB: 13060 ± 2301 pg/mL). Additionally, CaCl₂-activated PRP induced greater proliferation in ovarian fibroblasts (34.90 ± 17.82 ng/mL DNA) than collagen-activated PRP (30.75 ± 18.21 ng/mL DNA). In conclusion, the activation of PRP with CaCl₂ results in higher growth factor release and a stronger biological response compared to type I collagen-activation. These findings underscore the importance of standardized PRP activation protocols to enhance clinical outcomes in reproductive medicine.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"84"},"PeriodicalIF":3.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12023384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characteristics of early- and late-onset ovarian cancer: insights from multidimensional evidence.","authors":"Yanting Shen, Jie Cheng, Qing Ding, Zhihui Tao","doi":"10.1186/s13048-025-01664-9","DOIUrl":"https://doi.org/10.1186/s13048-025-01664-9","url":null,"abstract":"<p><strong>Background: </strong>Ovarian cancer (OC) is among the most lethal gynecologic malignancies, characterized by poor prognosis. While aging is a well-established risk factor, the underlying mechanisms distinguishing early- and late-onset ovarian cancer remain poorly understood.</p><p><strong>Methods: </strong>This study analyzed the global burden and age-related trends of ovarian cancer using the GBD database. A cut-off age of 55 years was used to differentiate between early and late onset ovarian cancer, and a Mendelian randomization method was also used to investigate the causal relationship between aging and ovarian cancer. Machine learning was applied to identify tumor-specific age-associated genes, followed by bioinformatics analyses and single-cell sequencing to explore the roles of these genes and immune profile alterations in ovarian cancer. Additionally, models were constructed, and drug sensitivity analyses performed to evaluate their potential as diagnostic markers or therapeutic targets.</p><p><strong>Results: </strong>Ovarian cancer incidence and mortality exhibit age-related trends, with telomere length positively associated with increased risk (OR = 1.27, 95% CI: 1.01-1.60, P = 3.90 × 10⁻²). Older patients with OC have a worse prognosis. PRKCD and UCP2 were significantly upregulated in ovarian cancer. PRKCD facilitates epithelial-mesenchymal transition (EMT), contributing to ovarian cancer progression, while UCP2 modulates ROS dynamics, influencing chemoresistance. Immune microenvironment analysis revealed differences between high- and low-expression groups, particularly in T cells, macrophages, and other immune cells. Both genes are sensitive to a varity of drugs, including dasatinib, fluvastatin, highlighting their potential as therapeutic targets.</p><p><strong>Conclusion: </strong>Aging is a significant risk factor for ovarian cancer, with PRKCD and UCP2 closely linked to its onset and progression. These genes show promise as novel biomarkers and therapeutic targets for ovarian cancer.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"83"},"PeriodicalIF":3.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NCAPH promotes glucose metabolism reprogramming and cell stemness in ovarian cancer cells through the MEK/ERK/PD-L1 pathway.","authors":"Yingying Qi, Aiping Wang, Silin Chen, Wei Chen","doi":"10.1186/s13048-025-01659-6","DOIUrl":"https://doi.org/10.1186/s13048-025-01659-6","url":null,"abstract":"<p><strong>Backgrounds: </strong>Ovarian cancer is a prevalent malignant tumor that affects the female reproductive system with the characteristic of high heterogeneity. Non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) has been implicated in a variety of cancers.</p><p><strong>Methods: </strong>The expression of NCAPH before and after transfection were assessed using RT-qPCR and western blot analysis. Cell stemness was evaluated through spheroid formation assay. The extracellular acidification rate (ECAR) of ovarian cancer cells was measured utilizing Seahorse Glycolysis Stress Test Assay while oxygen consumption rate (OCR) was estimated with Seahorse Mito Stress Test Assay. Lactate production and glucose consumption were quantified using corresponding assay kits. Western blot was employed to analyze the expression of stem cell markers, glycolysis- and MEK/ERK/PD-L1 signaling pathway-related proteins. In vivo, tumor size and weight were recorded, and immunohistochemical staining was used to assess MEK/ERK/PD-L1 and KI67 expression in tumor tissues from nude mice.</p><p><strong>Results: </strong>It was observed that NCAPH expression is upregulated in ovarian cancer cells. Silencing NCAPH led to repression of both stemness characteristics and glucose metabolism reprogramming. Furthermore, knockdown of NCAPH inhibited the MEK/ERK/PD-L1 signaling pathway both in vitro and in vivo, resulting in suppressed tumor growth in mouse models.</p><p><strong>Conclusion: </strong>Collectively, silencing NCAPH impedes malignant progression of ovarian cancer through modulation of the MEK/ERK/PD-L1 pathway.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"81"},"PeriodicalIF":3.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}