European Journal of Immunology最新文献_第6页

Tumor Glycosylation: A Main Player in the Modulation of Immune Responses 肿瘤糖基化：免疫反应调节的主要参与者

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-12 DOI: 10.1002/eji.202451318

Ernesto Rodriguez

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Differences in Phage Recognition and Immunogenicity Contribute to Divergent Human Immune Responses to Escherichia coli and Klebsiella pneumoniae Phages 噬菌体识别和免疫原性的差异导致人类对大肠杆菌和肺炎克雷伯菌噬菌体的不同免疫反应

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-12 DOI: 10.1002/eji.202451543

Huu Thanh Le, Carola Venturini, Alicia Fajardo Lubian, Bethany Bowring, Jonathan Iredell, Jacob George, Golo Ahlenstiel, Scott A. Read

{"title":"Differences in Phage Recognition and Immunogenicity Contribute to Divergent Human Immune Responses to Escherichia coli and Klebsiella pneumoniae Phages","authors":"Huu Thanh Le, Carola Venturini, Alicia Fajardo Lubian, Bethany Bowring, Jonathan Iredell, Jacob George, Golo Ahlenstiel, Scott A. Read","doi":"10.1002/eji.202451543","DOIUrl":"https://doi.org/10.1002/eji.202451543","url":null,"abstract":"Bacteriophages (phages) are emerging as a viable adjunct to antibiotics for the treatment of multidrug-resistant (MDR) bacterial infections. While intravenous phage therapy has proven successful in many cases, clinical outcomes remain uncertain due to a limited understanding of host response to phages. In this study, we conducted a comprehensive examination of the interaction between clinical-grade phages used to treat MDR Escherichia coli and Klebsiella pneumoniae infections, and human peripheral blood immune cells. Using whole transcriptome as well as proteomic approaches, we identified a strong inflammatory response to E. coli phage vB_EcoM-JIPh_Ec70 (herein, JIPh_Ec70) that was absent upon exposure to K. pneumoniae phage JIPh_Kp127. We confirmed that JIPh_Ec70's DNA recognition by the STING pathway was principally responsible for the activation of NF-kB and the subsequent inflammatory response. We further show that monocytes and neutrophils play a dominant role in phage uptake, primarily through complement-mediated phagocytosis. Significant differences in complement-mediated phagocytosis of JIPh_Kp127 and JIPh_Ec70 were observed, suggesting that reduced recognition, phagocytosis, and immunogenicity all contribute to the significantly decreased response to JIPh_Kp127. Our findings contribute to the progress of our understanding of the innate immune response to therapeutic phages and offer potential insights into how to improve the safety and effectiveness of phage therapy.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 3","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202451543","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Vaginal Prevotella timonensis Bacteria Enhance HIV-1 Uptake and Differentially Affect Transmission by Distinct Primary Dendritic Cell Subsets 阴道提蒙普氏菌增强HIV-1摄取并通过不同的初级树突状细胞亚群差异影响传播

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-12 DOI: 10.1002/eji.202451192

Marleen Y. van Smoorenburg, Ester B. M. Remmerswaal, Celia Segui-Perez, John L. van Hamme, Karin Strijbis, Teunis B. H. Geijtenbeek

{"title":"Vaginal Prevotella timonensis Bacteria Enhance HIV-1 Uptake and Differentially Affect Transmission by Distinct Primary Dendritic Cell Subsets","authors":"Marleen Y. van Smoorenburg, Ester B. M. Remmerswaal, Celia Segui-Perez, John L. van Hamme, Karin Strijbis, Teunis B. H. Geijtenbeek","doi":"10.1002/eji.202451192","DOIUrl":"https://doi.org/10.1002/eji.202451192","url":null,"abstract":"Young females are at high risk of acquiring HIV-1 infections and an imbalance in the vaginal microbiome enhances susceptibility to HIV-1 infection. More insights into the underlying mechanisms could open up new strategies to prevent HIV-1 acquisition and dissemination. Here, we investigated the effect of anaerobic bacteria associated with bacterial vaginosis (BV) on HIV-1 transmission by two distinct dendritic cell (DC) subsets, that is, inflammatory monocyte-derived DCs (moDCs) and primary CD1c+ DCs. Notably, in contrast to other BV-associated microbiota, Prevotella timonensis enhanced uptake of HIV-1 by both moDCs and CD1c+ DCs and the increased uptake was independent of cellular HIV-1 (co-)receptors. Imaging flow cytometry analyses showed that HIV-1 did not co-localise with P. timonensis but was internalized into tetraspanin-positive compartments known to be involved in HIV-1 transmission. P. timonensis bacteria enhanced HIV-1 transmission by CD1c+ DCs, but not by moDCs, and the enhanced transmission was independent of viral infection. Our study strongly suggests that mucosal DC subsets have distinct functions in BV-associated HIV-1 susceptibility, and underscores the importance of early diagnosis and targeted treatment of vaginal dysbiosis to reduce the risk of HIV-1 acquisition.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 3","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202451192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A Critical Oversight in Immunology Research: The Prevalence of Complement Deficiencies in Mouse Models 免疫学研究中的一个重要疏忽：小鼠模型中补体缺陷的普遍性

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-12 DOI: 10.1002/eji.202451678

África González-Fernández

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Issue Information: Eur. J. Immunol. 3'25 发行信息：欧元。[j] .免疫学杂志。3'25

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-10 DOI: 10.1002/eji.202570032

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Cover Story: Eur. J. Immunol. 3'25 封面故事：欧元。[j] .免疫学杂志。3'25

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-10 DOI: 10.1002/eji.202570031

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BTK-Inhibition Enhances TLR-7-Mediated Interferon-Alpha Production in pDCs by Blocking the Inhibitory BDCA-2 Pathway btk抑制通过阻断抑制BDCA-2途径增强tlr -7介导的pDCs中干扰素- α的产生

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-24 DOI: 10.1002/eji.202450985

Laura Ceglarek, Ramona Gerhards, Vinicius Boldrini, Christian Wichmann, Anneli Peters, Edgar Meinl

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Survival and Developmental Progression of Unselected Thymocytes in the Absence of the T-Cell Adaptor Gads 未选择胸腺细胞在缺乏t细胞接头Gads的情况下的存活和发育进展

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-24 DOI: 10.1002/eji.202451000

Rose Shalah, Manal Marzouk, Enas Hallumi, Naama Klopstock, Deborah Yablonski

{"title":"Survival and Developmental Progression of Unselected Thymocytes in the Absence of the T-Cell Adaptor Gads","authors":"Rose Shalah, Manal Marzouk, Enas Hallumi, Naama Klopstock, Deborah Yablonski","doi":"10.1002/eji.202451000","DOIUrl":"https://doi.org/10.1002/eji.202451000","url":null,"abstract":"Thymocyte β-selection and positive-selection depend on TCR signaling via the immune adaptors SLP-76 and LAT. Gads bridges the recruitment of SLP-76 to LAT, yet is not required for the maturation of single positive (SP) thymocytes. To illuminate this paradox, we performed tamoxifen-induced ablation of Gads (GadsiKO), accompanied by the expression of tdTomato, and compared the development of Gads-expressing (Tom−) and Gads-ablated (Tom+) thymocytes within the same mouse. GadsiKO (Tom+) thymocytes exhibited impaired β- and positive-selection, yet δ-selection was not affected. While susceptible to apoptosis ex vivo, the marked accumulation of self-MHC nonresponding (CD5−) GadsiKO DP thymocytes suggested the possibility of impaired death by neglect in situ. Further supporting this notion, GadsiKO CD5lo DP thymocytes exhibited reduced apoptosis in situ and reduced CD8-induced apoptosis ex vivo. Most GadsiKO CD4 SP thymocytes were positively selected, yet a distinct population of unselected (CD5− TCRβneg/low CCR7lo CD24hi) CD4 SP thymocytes was seen only in the absence of Gads. This unselected population did not include Treg or TCRγδ subsets; rather, it encompassed CD44lo CD25+ cells, resembling pre-β-selection thymocytes. Our results suggest that Gads promotes passage through key TCR-driven developmental checkpoints while repressing the progression of unselected DN and DP thymocytes.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 2","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202451000","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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VSTM1/SIRL-1: An Inhibitory Pattern Recognition Receptor Regulating Myeloid Cells VSTM1/SIRL-1：调节髓细胞的抑制性模式识别受体

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-24 DOI: 10.1002/eji.202451465

Maaike Koops, Linde Meyaard

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Trametinib Suppresses the Stimulated T Cells Through G1 Arrest and Apoptosis 曲美替尼通过G1阻滞和凋亡抑制受刺激的T细胞

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-24 DOI: 10.1002/eji.202350667

Toshimasa Nakao, Takero Shindo, Hideki Takakura, Takumi Narita, Yukako Ise-Nakao, Saeko Akiyama, Yosuke Iizumi, Shogen Boku, Motoki Watanabe, Toshiyuki Sakai, Seiichi Shimizu, Masaki Yamada, Yoshihiro Sowa, Michihiro Mutoh

{"title":"Trametinib Suppresses the Stimulated T Cells Through G1 Arrest and Apoptosis","authors":"Toshimasa Nakao, Takero Shindo, Hideki Takakura, Takumi Narita, Yukako Ise-Nakao, Saeko Akiyama, Yosuke Iizumi, Shogen Boku, Motoki Watanabe, Toshiyuki Sakai, Seiichi Shimizu, Masaki Yamada, Yoshihiro Sowa, Michihiro Mutoh","doi":"10.1002/eji.202350667","DOIUrl":"https://doi.org/10.1002/eji.202350667","url":null,"abstract":"<div>\u0000 \u0000 The development of efficient immunosuppressants may bring significant benefits to patients after organ/stem transplantation and those with allergies or autoimmune diseases. MEK inhibitors were originally developed as anticancer reagents, but recent reports have suggested that they may have the potential to be immunosuppressants. Trametinib is a first-in-class MEK inhibitor. Here, we examined the effects of trametinib on the immune system and revealed its mechanism. Trametinib suppressed both CD4 and CD8 T-cell proliferation and activated T cells, which expressed CD25 and TIM3, in a dose-dependent manner in vitro. Trametinib also suppressed T cell-related cytokine secretion in a dose-dependent manner. Notably, trametinib suppressed T cell proliferation through the induction of G1 arrest and apoptosis in stimulated T cells. In addition, trametinib induced regulatory T cells (Tregs). We confirmed that low concentrations of trametinib (1 and 10 nM) were not toxic toward splenic naïve T cells and normal mouse liver cells. In this study, we demonstrated whether trametinib suppressed CD4 and CD8 T cell proliferation by inducing G1 arrest and apoptosis along with suppression of cytokine secretion.\u0000 </div>","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 2","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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