European Journal of Immunology最新文献_第5页

Vaginal Prevotella timonensis Bacteria Enhance HIV-1 Uptake and Differentially Affect Transmission by Distinct Primary Dendritic Cell Subsets 阴道提蒙普氏菌增强HIV-1摄取并通过不同的初级树突状细胞亚群差异影响传播

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-12 DOI: 10.1002/eji.202451192

Marleen Y. van Smoorenburg, Ester B. M. Remmerswaal, Celia Segui-Perez, John L. van Hamme, Karin Strijbis, Teunis B. H. Geijtenbeek

{"title":"Vaginal Prevotella timonensis Bacteria Enhance HIV-1 Uptake and Differentially Affect Transmission by Distinct Primary Dendritic Cell Subsets","authors":"Marleen Y. van Smoorenburg, Ester B. M. Remmerswaal, Celia Segui-Perez, John L. van Hamme, Karin Strijbis, Teunis B. H. Geijtenbeek","doi":"10.1002/eji.202451192","DOIUrl":"https://doi.org/10.1002/eji.202451192","url":null,"abstract":"Young females are at high risk of acquiring HIV-1 infections and an imbalance in the vaginal microbiome enhances susceptibility to HIV-1 infection. More insights into the underlying mechanisms could open up new strategies to prevent HIV-1 acquisition and dissemination. Here, we investigated the effect of anaerobic bacteria associated with bacterial vaginosis (BV) on HIV-1 transmission by two distinct dendritic cell (DC) subsets, that is, inflammatory monocyte-derived DCs (moDCs) and primary CD1c+ DCs. Notably, in contrast to other BV-associated microbiota, Prevotella timonensis enhanced uptake of HIV-1 by both moDCs and CD1c+ DCs and the increased uptake was independent of cellular HIV-1 (co-)receptors. Imaging flow cytometry analyses showed that HIV-1 did not co-localise with P. timonensis but was internalized into tetraspanin-positive compartments known to be involved in HIV-1 transmission. P. timonensis bacteria enhanced HIV-1 transmission by CD1c+ DCs, but not by moDCs, and the enhanced transmission was independent of viral infection. Our study strongly suggests that mucosal DC subsets have distinct functions in BV-associated HIV-1 susceptibility, and underscores the importance of early diagnosis and targeted treatment of vaginal dysbiosis to reduce the risk of HIV-1 acquisition.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 3","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202451192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A Critical Oversight in Immunology Research: The Prevalence of Complement Deficiencies in Mouse Models 免疫学研究中的一个重要疏忽：小鼠模型中补体缺陷的普遍性

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-12 DOI: 10.1002/eji.202451678

África González-Fernández

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Issue Information: Eur. J. Immunol. 3'25 发行信息：欧元。[j] .免疫学杂志。3'25

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-10 DOI: 10.1002/eji.202570032

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Cover Story: Eur. J. Immunol. 3'25 封面故事：欧元。[j] .免疫学杂志。3'25

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-03-10 DOI: 10.1002/eji.202570031

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BTK-Inhibition Enhances TLR-7-Mediated Interferon-Alpha Production in pDCs by Blocking the Inhibitory BDCA-2 Pathway btk抑制通过阻断抑制BDCA-2途径增强tlr -7介导的pDCs中干扰素- α的产生

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-24 DOI: 10.1002/eji.202450985

Laura Ceglarek, Ramona Gerhards, Vinicius Boldrini, Christian Wichmann, Anneli Peters, Edgar Meinl

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Survival and Developmental Progression of Unselected Thymocytes in the Absence of the T-Cell Adaptor Gads 未选择胸腺细胞在缺乏t细胞接头Gads的情况下的存活和发育进展

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-24 DOI: 10.1002/eji.202451000

Rose Shalah, Manal Marzouk, Enas Hallumi, Naama Klopstock, Deborah Yablonski

{"title":"Survival and Developmental Progression of Unselected Thymocytes in the Absence of the T-Cell Adaptor Gads","authors":"Rose Shalah, Manal Marzouk, Enas Hallumi, Naama Klopstock, Deborah Yablonski","doi":"10.1002/eji.202451000","DOIUrl":"https://doi.org/10.1002/eji.202451000","url":null,"abstract":"Thymocyte β-selection and positive-selection depend on TCR signaling via the immune adaptors SLP-76 and LAT. Gads bridges the recruitment of SLP-76 to LAT, yet is not required for the maturation of single positive (SP) thymocytes. To illuminate this paradox, we performed tamoxifen-induced ablation of Gads (GadsiKO), accompanied by the expression of tdTomato, and compared the development of Gads-expressing (Tom−) and Gads-ablated (Tom+) thymocytes within the same mouse. GadsiKO (Tom+) thymocytes exhibited impaired β- and positive-selection, yet δ-selection was not affected. While susceptible to apoptosis ex vivo, the marked accumulation of self-MHC nonresponding (CD5−) GadsiKO DP thymocytes suggested the possibility of impaired death by neglect in situ. Further supporting this notion, GadsiKO CD5lo DP thymocytes exhibited reduced apoptosis in situ and reduced CD8-induced apoptosis ex vivo. Most GadsiKO CD4 SP thymocytes were positively selected, yet a distinct population of unselected (CD5− TCRβneg/low CCR7lo CD24hi) CD4 SP thymocytes was seen only in the absence of Gads. This unselected population did not include Treg or TCRγδ subsets; rather, it encompassed CD44lo CD25+ cells, resembling pre-β-selection thymocytes. Our results suggest that Gads promotes passage through key TCR-driven developmental checkpoints while repressing the progression of unselected DN and DP thymocytes.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 2","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202451000","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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VSTM1/SIRL-1: An Inhibitory Pattern Recognition Receptor Regulating Myeloid Cells VSTM1/SIRL-1：调节髓细胞的抑制性模式识别受体

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-24 DOI: 10.1002/eji.202451465

Maaike Koops, Linde Meyaard

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Trametinib Suppresses the Stimulated T Cells Through G1 Arrest and Apoptosis 曲美替尼通过G1阻滞和凋亡抑制受刺激的T细胞

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-24 DOI: 10.1002/eji.202350667

Toshimasa Nakao, Takero Shindo, Hideki Takakura, Takumi Narita, Yukako Ise-Nakao, Saeko Akiyama, Yosuke Iizumi, Shogen Boku, Motoki Watanabe, Toshiyuki Sakai, Seiichi Shimizu, Masaki Yamada, Yoshihiro Sowa, Michihiro Mutoh

{"title":"Trametinib Suppresses the Stimulated T Cells Through G1 Arrest and Apoptosis","authors":"Toshimasa Nakao, Takero Shindo, Hideki Takakura, Takumi Narita, Yukako Ise-Nakao, Saeko Akiyama, Yosuke Iizumi, Shogen Boku, Motoki Watanabe, Toshiyuki Sakai, Seiichi Shimizu, Masaki Yamada, Yoshihiro Sowa, Michihiro Mutoh","doi":"10.1002/eji.202350667","DOIUrl":"https://doi.org/10.1002/eji.202350667","url":null,"abstract":"<div>\u0000 \u0000 The development of efficient immunosuppressants may bring significant benefits to patients after organ/stem transplantation and those with allergies or autoimmune diseases. MEK inhibitors were originally developed as anticancer reagents, but recent reports have suggested that they may have the potential to be immunosuppressants. Trametinib is a first-in-class MEK inhibitor. Here, we examined the effects of trametinib on the immune system and revealed its mechanism. Trametinib suppressed both CD4 and CD8 T-cell proliferation and activated T cells, which expressed CD25 and TIM3, in a dose-dependent manner in vitro. Trametinib also suppressed T cell-related cytokine secretion in a dose-dependent manner. Notably, trametinib suppressed T cell proliferation through the induction of G1 arrest and apoptosis in stimulated T cells. In addition, trametinib induced regulatory T cells (Tregs). We confirmed that low concentrations of trametinib (1 and 10 nM) were not toxic toward splenic naïve T cells and normal mouse liver cells. In this study, we demonstrated whether trametinib suppressed CD4 and CD8 T cell proliferation by inducing G1 arrest and apoptosis along with suppression of cytokine secretion.\u0000 </div>","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 2","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Towards the Next Generation of Data-Driven Therapeutics Using Spatially Resolved Single-Cell Technologies and Generative AI 利用空间分辨单细胞技术和生成式人工智能迈向下一代数据驱动疗法

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-18 DOI: 10.1002/eji.202451234

Avital Rodov, Hosna Baniadam, Robert Zeiser, Ido Amit, Nir Yosef, Tobias Wertheimer, Florian Ingelfinger

{"title":"Towards the Next Generation of Data-Driven Therapeutics Using Spatially Resolved Single-Cell Technologies and Generative AI","authors":"Avital Rodov, Hosna Baniadam, Robert Zeiser, Ido Amit, Nir Yosef, Tobias Wertheimer, Florian Ingelfinger","doi":"10.1002/eji.202451234","DOIUrl":"https://doi.org/10.1002/eji.202451234","url":null,"abstract":"Recent advances in multi-omics and spatially resolved single-cell technologies have revolutionised our ability to profile millions of cellular states, offering unprecedented opportunities to understand the complex molecular landscapes of human tissues in both health and disease. These developments hold immense potential for precision medicine, particularly in the rational design of novel therapeutics for treating inflammatory and autoimmune diseases. However, the vast, high-dimensional data generated by these technologies present significant analytical challenges, such as distinguishing technical variation from biological variation or defining relevant questions that leverage the added spatial dimension to improve our understanding of tissue organisation. Generative artificial intelligence (AI), specifically variational autoencoder- or transformer-based latent variable models, provides a powerful and flexible approach to addressing these challenges. These models make inferences about a cell's intrinsic state by effectively identifying complex patterns, reducing data dimensionality and modelling the biological variability in single-cell datasets. This review explores the current landscape of single-cell and spatial multi-omics technologies, the application of generative AI in data analysis and modelling and their transformative impact on our understanding of autoimmune diseases. By combining spatial and single-cell data with advanced AI methodologies, we highlight novel insights into the pathogenesis of autoimmune disorders and outline future directions for leveraging these technologies to achieve the goal of AI-powered personalised medicine.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 2","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202451234","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Tissue-Resident Regulatory T Cells Expressing CD83 Maintain Local Homeostasis and Restrict Th2 Responses in Asthma 表达CD83的组织驻留调节性T细胞维持哮喘的局部稳态并限制Th2反应

IF 4.5 3区医学

European Journal of Immunology Pub Date : 2025-02-16 DOI: 10.1002/eji.202451525

Anita Heiß, Susanne Krammer, Christine Kuhnt, Christina Draßner, Philipp Beck, Adriana Geiger, Stefan Schliep, Carol-Immanuel Geppert, Alexander Steinkasserer, Andreas B. Wild

{"title":"Tissue-Resident Regulatory T Cells Expressing CD83 Maintain Local Homeostasis and Restrict Th2 Responses in Asthma","authors":"Anita Heiß, Susanne Krammer, Christine Kuhnt, Christina Draßner, Philipp Beck, Adriana Geiger, Stefan Schliep, Carol-Immanuel Geppert, Alexander Steinkasserer, Andreas B. Wild","doi":"10.1002/eji.202451525","DOIUrl":"https://doi.org/10.1002/eji.202451525","url":null,"abstract":"Non-lymphoid tissue Tregs (NLT-Tregs) are critical for tissue homeostasis, inflammation control, and induction of tissue repair. Recent single-cell RNA sequencing data identified the expression of CD83 as part of an NLT-Treg signature, which is an essential molecule for the stability and differentiation of lymphoid Tregs. However, the biological significance of CD83 expression for NLT Tregs has not yet been elucidated. The present study explores for the first time the role of CD83 expression by lung-resident Tregs in the steady state and during asthma to understand its importance in barrier tissues. We evaluated the effect of Treg-specific CD83 deletion (CD83cKO) on the lung-resident T-cell compartment and cytokine profile. CD83-deficient lung Tregs are less differentiated but more activated, resulting in unrestrained T-cell activation. Further, CD83cKO mice were challenged in an asthma model and showed an accelerated disease progression, driven by Th2-biased T-cell responses. CD83cKO Tregs exhibited enhanced responsiveness to IL-4, leading to insufficient control of Th2-differentiation from naïve T cells. These findings underscore the pivotal role of CD83 in the NLT-Treg-mediated modulation of Th2 responses. Overall, our results highlight CD83 as a key player in tissue homeostasis and inflammatory responses, suggesting potential therapeutic implications for inflammatory disorders such as asthma.","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 2","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202451525","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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