Journal of neurogenetics最新文献_第8页

Characterization of a novel stimulus-induced glial calcium wave in Drosophila larval peripheral segmental nerves and its role in PKG-modulated thermoprotection. 果蝇幼虫周围节段神经中刺激诱导的新型胶质钙波的特征及其在pkg调节的热保护中的作用。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-07-26 DOI: 10.1080/01677063.2021.1941945

Jennifer L Krill, Ken Dawson-Scully

{"title":"Characterization of a novel stimulus-induced glial calcium wave in Drosophila larval peripheral segmental nerves and its role in PKG-modulated thermoprotection.","authors":"Jennifer L Krill, Ken Dawson-Scully","doi":"10.1080/01677063.2021.1941945","DOIUrl":"https://doi.org/10.1080/01677063.2021.1941945","url":null,"abstract":"Insects, as poikilotherms, have adaptations to deal with wide ranges in temperature fluctuation. Allelic variations in the foraging gene that encodes a cGMP dependent protein kinase, were discovered to have effects on behavior in Drosophila by Dr. Marla Sokolowski in 1980. This single gene has many pleiotropic effects and influences feeding behavior, metabolic storage, learning and memory and has been shown to affect stress tolerance. PKG regulation affects motoneuronal thermotolerance in Drosophila larvae as well as adults. While the focus of thermotolerance studies has been on the modulation of neuronal function, other cell types have been overlooked. Because glia are vital to neuronal function and survival, we wanted to determine if glia play a role in thermotolerance as well. In our investigation, we discovered a novel calcium wave at the larval NMJ and set out to characterize the wave's dynamics and the potential mechanism underlying the wave prior to determining what effect, if any, PKG modulation has on the thermotolerance of glia cells. Using pharmacology, we determined that calcium buffering mechanisms of the mitochondria and endoplasmic reticulum play a role in the propagation of our novel glial calcium wave. By coupling pharmacology with genetic manipulation using RNA interference (RNAi), we found that PKG modulation in glia alters thermoprotection of function as well as glial calcium wave dynamics.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"221-235"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1941945","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39222786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Marla Sokolowski Retrospectively. Marla Sokolowski回顾一下。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-06-21 DOI: 10.1080/01677063.2021.1940169

Harold L Atwood

引用次数: 1

Gaining an understanding of behavioral genetics through studies of foraging in Drosophila and learning in C. elegans. 通过果蝇觅食和秀丽隐杆线虫学习的研究，获得对行为遗传学的理解。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-06-20 DOI: 10.1080/01677063.2021.1928113

Aaron P Reiss, Catharine H Rankin

{"title":"Gaining an understanding of behavioral genetics through studies of foraging in Drosophila and learning in C. elegans.","authors":"Aaron P Reiss, Catharine H Rankin","doi":"10.1080/01677063.2021.1928113","DOIUrl":"https://doi.org/10.1080/01677063.2021.1928113","url":null,"abstract":"The pursuit of understanding behavior has led to investigations of how genes, the environment, and the nervous system all work together to produce and influence behavior, giving rise to a field of research known as behavioral neurogenetics. This review focuses on the research journeys of two pioneers of aspects of behavioral neurogenetic research: Dr. Marla Sokolowski and Dr. Catharine Rankin as examples of how different approaches have been used to understand relationships between genes and behavior. Marla Sokolowski's research is centered around the discovery and analysis of foraging, a gene responsible for the natural behavioral polymorphism of Drosophila melanogaster larvae foraging behavior. Catharine Rankin's work began with demonstrating the ability to learn in Caenorhabditis elegans and then setting out to investigate the mechanisms underlying the \"simplest\" form of learning, habituation. Using these simple invertebrate organisms both investigators were able to perform in-depth dissections of behavior at genetic and molecular levels. By exploring their research and highlighting their findings we present ways their work has furthered our understanding of behavior and contributed to the field of behavioral neurogenetics.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"119-131"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1928113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39250993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The foraging gene as a modulator of division of labour in social insects. 群居昆虫觅食基因在劳动分工中的调节作用。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-06-20 DOI: 10.1080/01677063.2021.1940173

Christophe Lucas, Yehuda Ben-Shahar

{"title":"The foraging gene as a modulator of division of labour in social insects.","authors":"Christophe Lucas, Yehuda Ben-Shahar","doi":"10.1080/01677063.2021.1940173","DOIUrl":"https://doi.org/10.1080/01677063.2021.1940173","url":null,"abstract":"The social ants, bees, wasps, and termites include some of the most ecologically-successful groups of animal species. Their dominance in most terrestrial environments is attributed to their social lifestyle, which enable their colonies to exploit environmental resources with remarkable efficiency. One key attribute of social insect colonies is the division of labour that emerges among the sterile workers, which represent the majority of colony members. Studies of the mechanisms that drive division of labour systems across diverse social species have provided fundamental insights into the developmental, physiological, molecular, and genomic processes that regulate sociality, and the possible genetic routes that may have led to its evolution from a solitary ancestor. Here we specifically discuss the conserved role of the foraging gene, which encodes a cGMP-dependent protein kinase (PKG). Originally identified as a behaviourally polymorphic gene that drives alternative foraging strategies in the fruit fly Drosophila melanogaster, changes in foraging expression and kinase activity were later shown to play a key role in the division of labour in diverse social insect species as well. In particular, foraging appears to regulate worker transitions between behavioural tasks and specific behavioural traits associated with morphological castes. Although the specific neuroethological role of foraging in the insect brain remains mostly unknown, studies in genetically tractable insect species indicate that PKG signalling plays a conserved role in the neuronal plasticity of sensory, cognitive and motor functions, which underlie behaviours relevant to division of labour, including appetitive learning, aggression, stress response, phototaxis, and the response to pheromones.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"168-178"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1940173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39251177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Transcending boundaries: from quantitative genetics to single genes. 超越边界:从数量遗传学到单基因。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 DOI: 10.1080/01677063.2021.1960519

Jeffrey S Dason, Ina Anreiter, Chun-Fang Wu

引用次数: 0

Travels with Curly: A personal, collegial tribute to Professor Marla Sokolowski. 与卷毛一起旅行:对玛拉·索科洛夫斯基教授的个人、学院致敬。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-06-22 DOI: 10.1080/01677063.2021.1940174

W Thomas Boyce

引用次数: 1

Fly seizure EEG: field potential activity in the Drosophila brain. 苍蝇发作脑电图:果蝇大脑的场电位活动。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 DOI: 10.1080/01677063.2021.1950714

Atulya Iyengar, Chun-Fang Wu

{"title":"Fly seizure EEG: field potential activity in the Drosophila brain.","authors":"Atulya Iyengar, Chun-Fang Wu","doi":"10.1080/01677063.2021.1950714","DOIUrl":"https://doi.org/10.1080/01677063.2021.1950714","url":null,"abstract":"Hypersynchronous neural activity is a characteristic feature of seizures. Although many Drosophila mutants of epilepsy-related genes display clear behavioral spasms and motor unit hyperexcitability, field potential measurements of aberrant hypersynchronous activity across brain regions during seizures have yet to be described. Here, we report a straightforward method to observe local field potentials (LFPs) from the Drosophila brain to monitor ensemble neural activity during seizures in behaving tethered flies. High frequency stimulation across the brain reliably triggers a stereotypic sequence of electroconvulsive seizure (ECS) spike discharges readily detectable in the dorsal longitudinal muscle (DLM) and coupled with behavioral spasms. During seizure episodes, the LFP signal displayed characteristic large-amplitude oscillations with a stereotypic temporal correlation to DLM flight muscle spiking. ECS-related LFP events were clearly distinct from rest- and flight-associated LFP patterns. We further characterized the LFP activity during different types of seizures originating from genetic and pharmacological manipulations. In the 'bang-sensitive' sodium channel mutant bangsenseless (bss), the LFP pattern was prolonged, and the temporal correlation between LFP oscillations and DLM discharges was altered. Following administration of the pro-convulsant GABAA blocker picrotoxin, we uncovered a qualitatively different LFP activity pattern, which consisted of a slow (1-Hz), repetitive, waveform, closely coupled with DLM bursting and behavioral spasms. Our approach to record brain LFPs presents an initial framework for electrophysiological analysis of the complex brain-wide activity patterns in the large collection of Drosophila excitability mutants.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":"35 3","pages":"295-305"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1950714","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9113709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genetic architecture of larval aggregation behavior in Drosophila. 果蝇幼虫聚集行为的遗传结构。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-02-25 DOI: 10.1080/01677063.2021.1887174

Ross M McKinney, Ryan Valdez, Yehuda Ben-Shahar

{"title":"The genetic architecture of larval aggregation behavior in Drosophila.","authors":"Ross M McKinney, Ryan Valdez, Yehuda Ben-Shahar","doi":"10.1080/01677063.2021.1887174","DOIUrl":"https://doi.org/10.1080/01677063.2021.1887174","url":null,"abstract":"Many insect species exhibit basal social behaviors such as aggregation, which play important roles in their feeding and mating ecologies. However, the evolutionary, genetic, and physiological mechanisms that regulate insect aggregation remain unknown for most species. Here, we used natural populations of Drosophila melanogaster to identify the genetic architecture that drives larval aggregation feeding behavior. By using quantitative and reverse genetic approaches, we have identified a complex neurogenetic network that plays a role in regulating the decision of larvae to feed in either solitude or as a group. Results from single gene, RNAi-knockdown experiments show that several of the identified genes represent key nodes in the genetic network that determines the level of aggregation while feeding. Furthermore, we show that a single non-coding variant in the gene CG14205, a putative acyltransferase, is associated with both decreased mRNA expression and increased aggregate formation, which suggests that it has a specific role in inhibiting aggregation behavior. Our results identify, for the first time, the genetic components which interact to regulate naturally occurring levels of aggregation in D. melanogaster larvae.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"274-284"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1887174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25409896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The long view: a spouse's perspective. 长远的观点:配偶的观点。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-06-21 DOI: 10.1080/01677063.2021.1940170

Allen B Sokolowski

引用次数: 1

Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic orco mutation in honey bees. CRISPR/ cas9诱导的蜜蜂体细胞orco突变的神经发育和转录组效应

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-03-05 DOI: 10.1080/01677063.2021.1887173

Zhenqing Chen, Ian M Traniello, Seema Rana, Amy C Cash-Ahmed, Alison L Sankey, Che Yang, Gene E Robinson

{"title":"Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic orco mutation in honey bees.","authors":"Zhenqing Chen, Ian M Traniello, Seema Rana, Amy C Cash-Ahmed, Alison L Sankey, Che Yang, Gene E Robinson","doi":"10.1080/01677063.2021.1887173","DOIUrl":"https://doi.org/10.1080/01677063.2021.1887173","url":null,"abstract":"In insects, odorant receptors facilitate olfactory communication and require the functionality of the highly conserved co-receptor gene orco. Genome editing studies in a few species of ants and moths have revealed that orco can also have a neurodevelopmental function, in addition to its canonical role in adult olfaction, discovered first in Drosophila melanogaster. To extend this analysis, we determined whether orco mutations also affect the development of the adult brain of the honey bee Apis mellifera, an important model system for social behavior and chemical communication. We used CRISPR/Cas9 to knock out orco and examined anatomical and molecular consequences. To increase efficiency, we coupled embryo microinjection with a laboratory egg collection and in vitro rearing system. This new workflow advances genomic engineering technologies in honey bees by overcoming restrictions associated with field studies. We used Sanger sequencing to quickly select individuals with complete orco knockout for neuroanatomical analyses and later validated and described the mutations with amplicon sequencing. Mutant bees had significantly fewer glomeruli, smaller total volume of all the glomeruli, and higher mean individual glomerulus volume in the antennal lobe compared to wild-type controls. RNA-Sequencing revealed that orco knockout also caused differential expression of hundreds of genes in the antenna, including genes related to neural development and genes encoding odorant receptors. The expression of other types of chemoreceptor genes was generally unaffected, reflecting specificity of CRISPR activity in this study. These results suggest that neurodevelopmental effects of orco are related to specific insect life histories.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"320-332"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25432701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18