Journal of neurogenetics最新文献_第9页

Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic orco mutation in honey bees. CRISPR/ cas9诱导的蜜蜂体细胞orco突变的神经发育和转录组效应

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-03-05 DOI: 10.1080/01677063.2021.1887173

Zhenqing Chen, Ian M Traniello, Seema Rana, Amy C Cash-Ahmed, Alison L Sankey, Che Yang, Gene E Robinson

{"title":"Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic orco mutation in honey bees.","authors":"Zhenqing Chen, Ian M Traniello, Seema Rana, Amy C Cash-Ahmed, Alison L Sankey, Che Yang, Gene E Robinson","doi":"10.1080/01677063.2021.1887173","DOIUrl":"https://doi.org/10.1080/01677063.2021.1887173","url":null,"abstract":"In insects, odorant receptors facilitate olfactory communication and require the functionality of the highly conserved co-receptor gene orco. Genome editing studies in a few species of ants and moths have revealed that orco can also have a neurodevelopmental function, in addition to its canonical role in adult olfaction, discovered first in Drosophila melanogaster. To extend this analysis, we determined whether orco mutations also affect the development of the adult brain of the honey bee Apis mellifera, an important model system for social behavior and chemical communication. We used CRISPR/Cas9 to knock out orco and examined anatomical and molecular consequences. To increase efficiency, we coupled embryo microinjection with a laboratory egg collection and in vitro rearing system. This new workflow advances genomic engineering technologies in honey bees by overcoming restrictions associated with field studies. We used Sanger sequencing to quickly select individuals with complete orco knockout for neuroanatomical analyses and later validated and described the mutations with amplicon sequencing. Mutant bees had significantly fewer glomeruli, smaller total volume of all the glomeruli, and higher mean individual glomerulus volume in the antennal lobe compared to wild-type controls. RNA-Sequencing revealed that orco knockout also caused differential expression of hundreds of genes in the antenna, including genes related to neural development and genes encoding odorant receptors. The expression of other types of chemoreceptor genes was generally unaffected, reflecting specificity of CRISPR activity in this study. These results suggest that neurodevelopmental effects of orco are related to specific insect life histories.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"320-332"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25432701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

The Drosophila melanogaster foraging gene affects social networks. 黑腹果蝇的觅食基因影响着社会网络。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-06-12 DOI: 10.1080/01677063.2021.1936517

Nawar Alwash, Aaron M Allen, Marla B Sokolowski, Joel D Levine

{"title":"The Drosophila melanogaster foraging gene affects social networks.","authors":"Nawar Alwash, Aaron M Allen, Marla B Sokolowski, Joel D Levine","doi":"10.1080/01677063.2021.1936517","DOIUrl":"https://doi.org/10.1080/01677063.2021.1936517","url":null,"abstract":"Drosophila melanogaster displays social behaviors including courtship, mating, aggression, and group foraging. Recent studies employed social network analyses (SNAs) to show that D. melanogaster strains differ in their group behavior, suggesting that genes influence social network phenotypes. Aside from genes associated with sensory function, few studies address the genetic underpinnings of these networks. The foraging gene (for) is a well-established example of a pleiotropic gene that regulates multiple behavioral phenotypes and their plasticity. In D. melanogaster, there are two naturally occurring alleles of for called rover and sitter that differ in their larval and adult food-search behavior as well as other behavioral phenotypes. Here, we hypothesize that for affects behavioral elements required to form social networks and the social networks themselves. These effects are evident when we manipulate gene dosage. We found that flies of the rover and sitter strains exhibit differences in duration, frequency, and reciprocity of pairwise interactions, and they form social networks with differences in assortativity and global efficiency. Consistent with other adult phenotypes influenced by for, rover-sitter heterozygotes show intermediate patterns of dominance in many of these characteristics. Multiple generations of backcrossing a rover allele into a sitter strain showed that many but not all of these rover-sitter differences may be attributed to allelic variation at for. Our findings reveal the significant role that for plays in affecting social network properties and their behavioral elements in Drosophila melanogaster.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"249-261"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1936517","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39087650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Marla Sokolowski: and now for someone completely different. Marla Sokolowski:现在轮到一个完全不同的人了。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-07-14 DOI: 10.1080/01677063.2021.1940175

H Sofia Pereira, Karen D Williams, J Steven de Belle

引用次数: 2

Expression of the foraging gene in adult Drosophila melanogaster. 成年黑腹果蝇觅食基因的表达。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-08-12 DOI: 10.1080/01677063.2021.1941946

Aaron M Allen, Marla B Sokolowski

{"title":"Expression of the foraging gene in adult Drosophila melanogaster.","authors":"Aaron M Allen, Marla B Sokolowski","doi":"10.1080/01677063.2021.1941946","DOIUrl":"https://doi.org/10.1080/01677063.2021.1941946","url":null,"abstract":"The foraging gene in Drosophila melanogaster, which encodes a cGMP-dependent protein kinase, is a highly conserved, complex gene with multiple pleiotropic behavioral and physiological functions in both the larval and adult fly. Adult foraging expression is less well characterized than in the larva. We characterized foraging expression in the brain, gastric system, and reproductive systems using a T2A-Gal4 gene-trap allele. In the brain, foraging expression appears to be restricted to multiple sub-types of glia. This glial-specific cellular localization of foraging was supported by single-cell transcriptomic atlases of the adult brain. foraging is extensively expressed in most cell types in the gastric and reproductive systems. We then mapped multiple cis-regulatory elements responsible for parts of the observed expression patterns by a nested cloned promoter-Gal4 analysis. The mapped cis-regulatory elements were consistently modular when comparing the larval and adult expression patterns. These new data using the T2A-Gal4 gene-trap and cloned foraging promoter fusion GAL4's are discussed with respect to previous work using an anti-FOR antibody, which we show here to be non-specific. Future studies of foraging's function will consider roles for glial subtypes and peripheral tissues (gastric and reproductive systems) in foraging's pleiotropic behavioral and physiological effects.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"192-212"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39305650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Generation and characterization of fruitless P1 promoter mutant in Drosophila melanogaster. 黑腹果蝇P1无果启动子突变体的产生及特性研究。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 DOI: 10.1080/01677063.2021.1931179

Megan C Neville, Alexander Eastwood, Aaron M Allen, Ammerins de Haan, Tetsuya Nojima, Stephen F Goodwin

{"title":"Generation and characterization of fruitless P1 promoter mutant in Drosophila melanogaster.","authors":"Megan C Neville, Alexander Eastwood, Aaron M Allen, Ammerins de Haan, Tetsuya Nojima, Stephen F Goodwin","doi":"10.1080/01677063.2021.1931179","DOIUrl":"https://doi.org/10.1080/01677063.2021.1931179","url":null,"abstract":"The identification of mutations in the gene fruitless (fru) paved the way for understanding the genetic basis of male sexual behavior in the vinegar fly Drosophila melanogaster. D. melanogaster males perform an elaborate courtship display to the female, ultimately leading to copulation. Mutations in fru have been shown to disrupt most aspects of the male's behavioral display, rendering males behaviorally sterile. The fru genomic locus encodes for multiple transcription factor isoforms from several promoters; only those under the regulation of the most distal P1 promoter are under the control of the sex determination hierarchy and play a role in male-specific behaviors. In this study, we used CRISPR/Cas9-based targeted genome editing of the fru gene, to remove the P1 promoter region. We have shown that removal of the P1 promoter leads to a dramatic decrease in male courtship displays towards females and male-specific sterility. We have expanded the analysis of fru P1-dependent behaviors, examining male's response to courtship song and general activity levels during12-hour light: dark cycles. Our novel allele expands the mutant repertoire available for future studies of fru P1-derived function in D. melanogaster. Our fruΔP1 mutant will be useful for future studies of fru P1-derived function, as it can be homozygosed without disrupting additional downstream promoter function and can be utilized in heterozygous combinations with other extant fru alleles.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":"35 3","pages":"285-294"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1931179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9311488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Drosophila foraging gene plays a vital role at the start of metamorphosis for subsequent adult emergence. 果蝇的觅食基因在果蝇蜕变的开始和随后成虫的出现中起着至关重要的作用。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-05-04 DOI: 10.1080/01677063.2021.1914608

Ina Anreiter, Aaron M Allen, Oscar E Vasquez, Lydia To, Scott J Douglas, Javier V Alvarez, John Ewer, Marla B Sokolowski

{"title":"The Drosophila foraging gene plays a vital role at the start of metamorphosis for subsequent adult emergence.","authors":"Ina Anreiter, Aaron M Allen, Oscar E Vasquez, Lydia To, Scott J Douglas, Javier V Alvarez, John Ewer, Marla B Sokolowski","doi":"10.1080/01677063.2021.1914608","DOIUrl":"https://doi.org/10.1080/01677063.2021.1914608","url":null,"abstract":"The foraging (for) gene has been extensively studied in many species for its functions in development, physiology, and behavior. It is common for genes that influence behavior and development to be essential genes, and for has been found to be an essential gene in both fruit flies and mammals, with for mutants dying before reaching the adult stage. However, the biological process underlying the lethality associated with this gene is not known. Here, we show that in Drosophila melanogaster, some but not all gene products of for are essential for survival. Specifically, we show that promoter 3 of for, but not promoters 1, 2, and 4 are required for survival past pupal stage. We use full and partial genetic deletions of for, and temperature-restricted knock-down of the gene to further investigate the stage of lethality. While deletion analysis shows that flies lacking for die at the end of pupal development, as pharate adults, temperature-restricted knock-down shows that for is only required at the start of pupal development, for normal adult emergence (AE) and viability. We further show that the inability of these mutants to emerge from their pupal cases is linked to deficiencies in emergence behaviors, caused by a possible energy deficiency, and finally, that the lethality of for mutants seems to be linked to protein isoform P3, transcribed from for promoter 3.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"179-191"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1914608","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38946552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Learning about quantitative genetics from Marla Sokolowski. 向Marla Sokolowski学习定量遗传学。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-06-15 DOI: 10.1080/01677063.2021.1940167

Ralph J Greenspan

引用次数: 1

Dysregulation of Myt1 expression acts as a potential peripheral biomarker for major depressive disorder and bipolar disorder. Myt1表达失调是重度抑郁症和双相情感障碍的潜在外周生物标志物。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-05-20 DOI: 10.1080/01677063.2021.1928663

Maryam Ghanbarirad, Mehrdad Hashemi, Seyed Mehdi Saberi, Ahmad Majd

{"title":"Dysregulation of Myt1 expression acts as a potential peripheral biomarker for major depressive disorder and bipolar disorder.","authors":"Maryam Ghanbarirad, Mehrdad Hashemi, Seyed Mehdi Saberi, Ahmad Majd","doi":"10.1080/01677063.2021.1928663","DOIUrl":"https://doi.org/10.1080/01677063.2021.1928663","url":null,"abstract":"Major depressive disorder (MDD) and bipolar disorder (BPD) are among the most debilitating mental conditions. Diagnostic criteria for MDD include psychological and physical symptoms, such as low mood and changes in appetite or sleep, respectively. BPD in addition to periods of depression represents episodes of mania or hypomania, and elevation in mood and energy levels are associated with this condition. Dysregulation in adult neurogenesis and myelination have been reported in psychiatric disorders. As a key factor in neurogenesis, it was hypothesized that Myt1 gene expression may be altered in these conditions. Using Real-time PCR, Myt1 expression level in 100 MDD patients and 100 BPD patients, compared with healthy control (HC) individuals was evaluated. Results demonstrate significant downregulation of Myt1 in MDD and BPD. Logistic regression analysis and binary classification evaluation reveal potential risk factor and biomarker characteristics of Myt1, respectively. Moreover, forward and backward digit span results denote a significant reduction in the function of working memory (WM) of MDD and BPD subjects. Correlation analysis revealed a significant association between Myt1 downregulation and WM disruption in the affected individuals. In conclusion, due to its altered role in neurogenesis, downregulation of Myt1 can be associated with the pathology of MDD and BPD.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"381-386"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1928663","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38916619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A latitudinal cline in a courtship song character of Drosophila melanogaster. 黑腹果蝇求偶鸣声特征的纬度变化。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-06-08 DOI: 10.1080/01677063.2021.1933968

Rosamund A Stanley, Charalambos P Kyriacou

{"title":"A latitudinal cline in a courtship song character of Drosophila melanogaster.","authors":"Rosamund A Stanley, Charalambos P Kyriacou","doi":"10.1080/01677063.2021.1933968","DOIUrl":"https://doi.org/10.1080/01677063.2021.1933968","url":null,"abstract":"The courtship song of male Drosophila melanogaster is generated by wing vibration and contains an interpulse interval (IPI) which is species-specific and usually falls in the mean range of 30-40 ms. The IPI is extremely temperature-sensitive, so we wondered whether flies collected along the eastern coast of Australia between latitudes 16.9°S and 42.9°S might have adapted to the different thermal conditions and show differences in mean IPI. We observe a significant correlation between IPI and latitude in addition to the well-known association between latitude and body size (Bergmannn's Rule). However, somewhat surprisingly we could not detect a significant association between body size and IPI. We also examined flies collected from the North and South-facing slopes of 'Evolution Canyon' in Israel and observed differences in IPI that support the view that thermal adaptation can shape this important song character. We also examined the songs of flies from Kenya and observed no correlation between altitude of collection and IPI. In all three experiments, body size did not correlate with IPI. A global analysis of all three sets of populations on three continents revealed a strong association between IPI and latitude. We speculate that IPI is shaped by thermal and sexual selection whereas body size is also shaped by natural selection.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":" ","pages":"333-340"},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2021.1933968","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39072558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Women in science: a son's perspective. 科学领域的女性:一个儿子的视角。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-06-12 DOI: 10.1080/01677063.2021.1940171

Dustin J Sokolowski

引用次数: 1