Journal of neurogenetics最新文献_第10页

The OptoGenBox - a device for long-term optogenetics in C. elegans. OptoGenBox -秀丽隐杆线虫的长期光遗传学设备。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 Epub Date: 2020-06-16 DOI: 10.1080/01677063.2020.1776709

Inka Busack, Florian Jordan, Peleg Sapir, Henrik Bringmann

{"title":"The OptoGenBox - a device for long-term optogenetics in C. elegans.","authors":"Inka Busack, Florian Jordan, Peleg Sapir, Henrik Bringmann","doi":"10.1080/01677063.2020.1776709","DOIUrl":"https://doi.org/10.1080/01677063.2020.1776709","url":null,"abstract":"Optogenetics controls neural activity and behavior in living organisms through genetically targetable actuators and light. This method has revolutionized biology and medicine as it allows controlling cells with high temporal and spatial precision. Optogenetics is typically applied only at short time scales, for instance to study specific behaviors. Optogenetically manipulating behavior also gives insights into physiology, as behavior controls systemic physiological processes. For example, arousal and sleep affect aging and health span. To study how behavior controls key physiological processes, behavioral manipulations need to occur at extended time scales. However, methods for long-term optogenetics are scarce and typically require expensive compound microscope setups. Optogenetic experiments can be conducted in many species. Small model animals such as the nematode C. elegans have been instrumental in solving the mechanistic basis of medically important biological processes. We developed the OptoGenBox, an affordable stand-alone and simple-to-use device for long-term optogenetic manipulation of C. elegans. The OptoGenBox provides a controlled environment and is programmable to allow the execution of complex optogenetic manipulations over long experimental times of many days to weeks. To test our device, we investigated how optogenetically increased arousal and optogenetic sleep deprivation affect survival of arrested first larval stage C. elegans. We optogenetically activated the nociceptive ASH sensory neurons using ReaChR, thus triggering an escape response and increase in arousal. In addition, we optogenetically inhibited the sleep neuron RIS using ArchT, a condition known to impair sleep. Both optogenetic manipulations reduced survival. Thus, the OptoGenBox presents an affordable system to study the long-term consequences of optogenetic manipulations of key biological processes in C. elegans and perhaps other small animals.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2020.1776709","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38050626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Neuroendocrine control of lipid metabolism: lessons from C. elegans. 脂质代谢的神经内分泌控制:秀丽隐杆线虫的启示。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 Epub Date: 2020-07-03 DOI: 10.1080/01677063.2020.1777116

Supriya Srinivasan

引用次数: 8

Host-microbe interactions and the behavior of Caenorhabditis elegans. 宿主-微生物相互作用和秀丽隐杆线虫的行为。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 Epub Date: 2020-08-12 DOI: 10.1080/01677063.2020.1802724

Dennis H Kim, Steven W Flavell

{"title":"Host-microbe interactions and the behavior of Caenorhabditis elegans.","authors":"Dennis H Kim, Steven W Flavell","doi":"10.1080/01677063.2020.1802724","DOIUrl":"https://doi.org/10.1080/01677063.2020.1802724","url":null,"abstract":"Microbes are ubiquitous in the natural environment of Caenorhabditis elegans. Bacteria serve as a food source for C. elegans but may also cause infection in the nematode host. The sensory nervous system of C. elegans detects diverse microbial molecules, ranging from metabolites produced by broad classes of bacteria to molecules synthesized by specific strains of bacteria. Innate recognition through chemosensation of bacterial metabolites or mechanosensation of bacteria can induce immediate behavioral responses. The ingestion of nutritive or pathogenic bacteria can modulate internal states that underlie long-lasting behavioral changes. Ingestion of nutritive bacteria leads to learned attraction and exploitation of the bacterial food source. Infection, which is accompanied by activation of innate immunity, stress responses, and host damage, leads to the development of aversive behavior. The integration of a multitude of microbial sensory cues in the environment is shaped by experience and context. Genetic, chemical, and neuronal studies of C. elegans behavior in the presence of bacteria have defined neural circuits and neuromodulatory systems that shape innate and learned behavioral responses to microbial cues. These studies have revealed the profound influence that host-microbe interactions have in governing the behavior of this simple animal host.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2020.1802724","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38261724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Intraguild predation between Pristionchus pacificus and Caenorhabditis elegans: a complex interaction with the potential for aggressive behaviour. Pristionchus pacificus 和 Caenorhabditis elegans 之间的群落内捕食：具有潜在攻击行为的复杂互动。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 Epub Date: 2020-10-15 DOI: 10.1080/01677063.2020.1833004

Kathleen T Quach, Sreekanth H Chalasani

{"title":"Intraguild predation between Pristionchus pacificus and Caenorhabditis elegans: a complex interaction with the potential for aggressive behaviour.","authors":"Kathleen T Quach, Sreekanth H Chalasani","doi":"10.1080/01677063.2020.1833004","DOIUrl":"10.1080/01677063.2020.1833004","url":null,"abstract":"The related nematodes Pristionchus pacificus and Caenorhabditis elegans both eat bacteria for nutrition and are therefore competitors when they exploit the same bacterial resource. In addition to competing with each other, P. pacificus is a predator of C. elegans larval prey. These two relationships together form intraguild predation, which is the killing and sometimes eating of potential competitors. In killing C. elegans, the intraguild predator P. pacificus may achieve dual benefits of immediate nutrition and reduced competition for bacteria. Recent studies of P. pacificus have characterized many aspects of its predatory biting behaviour as well as underlying molecular and genetic mechanisms. However, little has been explored regarding the potentially competitive aspect of P. pacificus biting C. elegans. Moreover, aggression may also be implicated if P. pacificus intentionally bites C. elegans with the goal of reducing competition for bacteria. The aim of this review is to broadly outline how aggression, predation, and intraguild predation relate to each other, as well as how these concepts may be applied to future studies of P. pacificus in its interactions with C. elegans.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836027/pdf/nihms-1660112.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38489757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature signaling underlying thermotaxis and cold tolerance in Caenorhabditis elegans. 秀丽隐杆线虫致热性和耐寒性的温度信号。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 Epub Date: 2020-04-21 DOI: 10.1080/01677063.2020.1734001

Asuka Takeishi, Natsune Takagaki, Atsushi Kuhara

{"title":"Temperature signaling underlying thermotaxis and cold tolerance in Caenorhabditis elegans.","authors":"Asuka Takeishi, Natsune Takagaki, Atsushi Kuhara","doi":"10.1080/01677063.2020.1734001","DOIUrl":"https://doi.org/10.1080/01677063.2020.1734001","url":null,"abstract":"Caenorhabditis elegans has a simple nervous system of 302 neurons. It however senses environmental cues incredibly precisely and produces various behaviors by processing information in the neural circuit. In addition to classical genetic analysis, fluorescent proteins and calcium indicators enable in vivo monitoring of protein dynamics and neural activity on either fixed or free-moving worms. These analyses have provided the detailed molecular mechanisms of neuronal and systemic signaling that regulate worm responses. Here, we focus on responses of C. elegans against temperature and review key findings that regulate thermotaxis and cold tolerance. Thermotaxis of C. elegans has been studied extensively for almost 50 years, and cold tolerance is a relatively recent concept in C. elegans. Although both thermotaxis and cold tolerance require temperature sensation, the responsible neurons and molecular pathways are different, and C. elegans uses the proper mechanisms depending on its situation. We summarize the molecular mechanisms of the major thermosensory circuit as well as the modulatory strategy through neural and tissue communication that enables fine tuning of thermotaxis and cold tolerance.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2020.1734001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37856059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

The contribution of C. elegans neurogenetics to understanding neurodegenerative diseases. 秀丽隐杆线虫神经遗传学对理解神经退行性疾病的贡献。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 Epub Date: 2020-08-08 DOI: 10.1080/01677063.2020.1803302

Joseph J H Liang, Issa A McKinnon, Catharine H Rankin

{"title":"The contribution of C. elegans neurogenetics to understanding neurodegenerative diseases.","authors":"Joseph J H Liang, Issa A McKinnon, Catharine H Rankin","doi":"10.1080/01677063.2020.1803302","DOIUrl":"https://doi.org/10.1080/01677063.2020.1803302","url":null,"abstract":"Since Caenorhabditis elegans was first introduced as a genetic model organism by Sydney Brenner, researchers studying it have made significant contributions in numerous fields including investigations of the pathophysiology of neurodegenerative diseases. The simple anatomy, optical transparency, and short life-span of this small nematode together with the development and curation of many openly shared resources (including the entire genome, cell lineage and the neural map of the animal) allow researchers using C. elegans to move their research forward rapidly in an immensely collaborative community. These resources have allowed researchers to use C. elegans to study the cellular processes that may underlie human diseases. Indeed, many disease-associated genes have orthologs in C. elegans, allowing the effects of mutations in these genes to be studied in relevant and reproducible neuronal cell-types at single-cell resolution in vivo. Here we review studies that have attempted to establish genetic models of specific human neurodegenerative diseases (ALS, Alzheimer's Disease, Parkinson's Disease, Huntington's Disease) in C. elegans and what they have contributed to understanding the molecular and genetic underpinnings of each disease. With continuous advances in genome engineering, research conducted using this small organism first established by Brenner, Sulston and their contemporaries will continue to contribute to the understanding of human nervous diseases.","PeriodicalId":16491,"journal":{"name":"Journal of neurogenetics","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01677063.2020.1803302","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38245749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

My life with Sydney, 1961-1971. 我在悉尼的生活，1961-1971。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 DOI: 10.1080/01677063.2020.1834544

Antony O W Stretton

引用次数: 0

But can they learn? My accidental discovery of learning and memory in C. elegans. 但是他们能学习吗?我偶然发现秀丽隐杆线虫的学习和记忆能力。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 DOI: 10.1080/01677063.2020.1833009

Catharine H Rankin

引用次数: 2

Neuromodulators: an essential part of survival. 神经调节剂：生存的重要组成部分。

IF 1.8 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 Epub Date: 2020-11-10 DOI: 10.1080/01677063.2020.1839066

Joy Alcedo, Veena Prahlad

引用次数: 0

A journey to 'tame a small metazoan organism', ^‡ seen through the artistic eyes of C. elegans researchers. 从 elegans 研究人员的艺术视角看 "驯服小型后生动物 "之旅‡。

IF 1.9 4区医学

Journal of neurogenetics Pub Date : 2020-09-01 Epub Date: 2020-12-08 DOI: 10.1080/01677063.2020.1839449

Eleni Gourgou, Alexandra R Willis, Sebastian Giunti, Maria J De Rosa, Amanda G Charlesworth, Mirella Hernandez Lima, Elizabeth Glater, Sonja Soo, Bianca Pereira, Kübra Akbaş, Anushka Deb, Madhushree Kamak, Mark W Moyle, Annika Traa, Aakanksha Singhvi, Surojit Sural, Eugene Jennifer Jin

引用次数: 4