The Drosophila foraging gene plays a vital role at the start of metamorphosis for subsequent adult emergence.

IF 1.8 4区 医学 Q3 GENETICS & HEREDITY
Journal of neurogenetics Pub Date : 2021-09-01 Epub Date: 2021-05-04 DOI:10.1080/01677063.2021.1914608
Ina Anreiter, Aaron M Allen, Oscar E Vasquez, Lydia To, Scott J Douglas, Javier V Alvarez, John Ewer, Marla B Sokolowski
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引用次数: 6

Abstract

The foraging (for) gene has been extensively studied in many species for its functions in development, physiology, and behavior. It is common for genes that influence behavior and development to be essential genes, and for has been found to be an essential gene in both fruit flies and mammals, with for mutants dying before reaching the adult stage. However, the biological process underlying the lethality associated with this gene is not known. Here, we show that in Drosophila melanogaster, some but not all gene products of for are essential for survival. Specifically, we show that promoter 3 of for, but not promoters 1, 2, and 4 are required for survival past pupal stage. We use full and partial genetic deletions of for, and temperature-restricted knock-down of the gene to further investigate the stage of lethality. While deletion analysis shows that flies lacking for die at the end of pupal development, as pharate adults, temperature-restricted knock-down shows that for is only required at the start of pupal development, for normal adult emergence (AE) and viability. We further show that the inability of these mutants to emerge from their pupal cases is linked to deficiencies in emergence behaviors, caused by a possible energy deficiency, and finally, that the lethality of for mutants seems to be linked to protein isoform P3, transcribed from for promoter 3.

果蝇的觅食基因在果蝇蜕变的开始和随后成虫的出现中起着至关重要的作用。
觅食(for)基因在许多物种中因其在发育、生理和行为方面的功能而被广泛研究。影响行为和发育的基因通常是必不可少的基因,在果蝇和哺乳动物中都发现了一个必不可少的基因,突变体在到达成年阶段之前就死亡了。然而,与该基因相关的致死性的生物学过程尚不清楚。在这里,我们表明,在果蝇中,一些但不是全部的基因产物是生存所必需的。具体地说,我们表明启动子3,而不是启动子1、2和4是存活过蛹期所必需的。我们使用for的全部和部分基因缺失,以及温度限制性基因敲除来进一步研究致死阶段。虽然缺失分析表明,果蝇在蛹发育结束时缺乏for,但作为成熟成虫,温度限制敲除表明,for仅在蛹发育开始时需要,用于正常的成虫羽化(AE)和生存能力。我们进一步表明,这些突变体无法从它们的蛹中出现与出现行为的缺陷有关,这可能是由能量缺乏引起的,最后,突变体的致命性似乎与启动子3转录的蛋白质异构体P3有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of neurogenetics
Journal of neurogenetics 医学-神经科学
CiteScore
4.40
自引率
0.00%
发文量
13
审稿时长
>12 weeks
期刊介绍: The Journal is appropriate for papers on behavioral, biochemical, or cellular aspects of neural function, plasticity, aging or disease. In addition to analyses in the traditional genetic-model organisms, C. elegans, Drosophila, mouse and the zebrafish, the Journal encourages submission of neurogenetic investigations performed in organisms not easily amenable to experimental genetics. Such investigations might, for instance, describe behavioral differences deriving from genetic variation within a species, or report human disease studies that provide exceptional insights into biological mechanisms
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