Journal of Neuroscience Research最新文献_第7页

Oral pre- and early postnatal cannabis exposure disinhibits ventral tegmental area dopamine neuron activity but does not influence cocaine preference in offspring in mice 出生前和出生后早期口服大麻会抑制腹侧被盖区多巴胺神经元的活动，但不会影响小鼠后代对可卡因的偏好。

IF 2.9 3区医学

Journal of Neuroscience Research Pub Date : 2024-07-22 DOI: 10.1002/jnr.25369

Colleen S. Peterson, Samantha L. Baglot, Nada A. Sallam, Sarah Mina, Matthew N. Hill, Stephanie L. Borgland

{"title":"Oral pre- and early postnatal cannabis exposure disinhibits ventral tegmental area dopamine neuron activity but does not influence cocaine preference in offspring in mice","authors":"Colleen S. Peterson, Samantha L. Baglot, Nada A. Sallam, Sarah Mina, Matthew N. Hill, Stephanie L. Borgland","doi":"10.1002/jnr.25369","DOIUrl":"10.1002/jnr.25369","url":null,"abstract":"Cannabis consumption has increased from 1.5% to 2.5% in Canada between 2012 and 2019. Clinical studies have indicated effects of prenatal cannabis exposure on birth weight, substance use, and neurodevelopmental disorders, but are confounded by several difficult to control variables. Animal models allow for examination of the mechanism of cannabis-induced changes in neurodevelopment and behavior, while controlling dose and timing. Several animal models of prenatal cannabis exposure exist which provide varying levels of construct validity, control of dose, and exposure to maternal stress. Using a voluntary oral consumption model, mouse dams received 5 mg/kg Δ9-tetrahydrocannabinol (THC) whole cannabis oil in peanut butter daily from gestational day 1 (GD1) to postnatal day 10 (PD10). At GD1, GD18, PD1, PD10, and PD15, maternal plasma was collected; pup brains were collected from GD18 onward. Pup brains had higher levels of THC and cannabidiol at each time point, each of which persisted in maternal plasma and pup brains past the end of treatment (PD15). Male and female adolescent offspring were examined for changes to ventral tegmental area (VTA) dopamine neuron activity and cocaine-seeking behavior. Prenatal and early postnatal (GD1–PD10) cannabis-exposed male, but not female mice had decreased gamma-aminobutyric acid (GABAergic) input, depolarized resting membrane potential, and increased spontaneous firing of VTA dopamine neurons. Cannabis-exposed offspring showed faster decay of N-methyl-D-aspartate (NMDA) currents in both sexes. However, no differences in cocaine-seeking behavior were noted. These data characterize a voluntary prenatal cannabis exposure model and demonstrates VTA dopamine neuronal activity is disinhibited in offspring.","PeriodicalId":16490,"journal":{"name":"Journal of Neuroscience Research","volume":"102 7","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jnr.25369","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating social communication in mice: A two-intruders test approach 研究小鼠的社会交流：双入侵者测试法

IF 2.9 3区医学

Journal of Neuroscience Research Pub Date : 2024-07-19 DOI: 10.1002/jnr.25365

Maryana V. Morozova, Lidiya V. Boldyreva, Maria A. Borisova, Elena N. Kozhevnikova

{"title":"Investigating social communication in mice: A two-intruders test approach","authors":"Maryana V. Morozova, Lidiya V. Boldyreva, Maria A. Borisova, Elena N. Kozhevnikova","doi":"10.1002/jnr.25365","DOIUrl":"https://doi.org/10.1002/jnr.25365","url":null,"abstract":"Understanding the complex dynamics of social communication behaviors, such as exploration, communication, courtship, mating, and aggression in animal models, is crucial to reveal key neural and hormonal mechanisms underlying these behaviors. The two-intruders test is designed to investigate residents' behavior toward both male and female intruders within the home cage of the test male. During this test imitating natural conditions, several aspects of social interaction were investigated: Exploration, courtship, mating, and aggressive behavior. As mating and aggression involve overlapping neural circuits, the behavioral setup testing both behaviors is best at reflecting their competitive nature. Our findings demonstrate that resident male mice exhibit strong preference to communicate with a female intruder, which correlates with baseline testosterone levels of test males. Relevant female preference in the two-intruders test was also found in BALB/c males. Behavioral breakdown revealed the anogenital sniffing as a key behavioral feature that discriminates resident male behavior toward intruders of different sex. Furthermore, resident male interaction with female intruder was accompanied by neuronal activation in the ventromedial hypothalamus. We demonstrate that odor recognition underlies preference toward females in male residents, as experimental anosmia reduced communication with a female intruder. We conclude the two-intruders test setup to be a useful tool to study the neurological basis of social communication in animal models, which provides detailed analysis of various aspects of the laboratory animals’ social behavior in the most natural conditions.","PeriodicalId":16490,"journal":{"name":"Journal of Neuroscience Research","volume":"102 7","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141730116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review of MRI studies on the effects of maternal obesity on offspring brain structure and function 关于母体肥胖对后代大脑结构和功能影响的磁共振成像研究的系统回顾。

IF 2.9 3区医学

Journal of Neuroscience Research Pub Date : 2024-07-15 DOI: 10.1002/jnr.25368

Mohammadamin Parsaei, Seyedeh Melika Hashemi, Hossein Sanjari Moghaddam, Bradley S. Peterson

{"title":"A systematic review of MRI studies on the effects of maternal obesity on offspring brain structure and function","authors":"Mohammadamin Parsaei, Seyedeh Melika Hashemi, Hossein Sanjari Moghaddam, Bradley S. Peterson","doi":"10.1002/jnr.25368","DOIUrl":"10.1002/jnr.25368","url":null,"abstract":"Maternal obesity before or during pregnancy has been associated previously in offspring with a wide range of poor neurodevelopmental outcomes and mental health problems. The effects of maternal obesity on offspring brain structure and function that may be responsible for these poor outcomes are not well understood. We, therefore, undertook a systematic review of magnetic resonance imaging (MRI) studies that have assessed the associations of maternal obesity with brain measures in offspring. A systematic search was conducted in PubMed, Web of Science, Scopus, and PsycINFO on August 20, 2023. Of 15 eligible studies, seven employed functional MRI (fMRI), five diffusion tensor imaging (DTI), and four anatomical MRI (one used both DTI and anatomical MRI) in the offspring. The ages of offspring varied widely: one was a study of fetuses in utero, five of neonates, one of infants, five of school-aged children, two of both neonates and infants, and one of both children and adults. Collectively, 12 studies reported significant associations of maternal obesity with structural or functional alterations of the offspring's brain, most frequently in the prefrontal cortex and limbic system. In conclusion, maternal obesity appears to have a profound influence on offspring brain development, particularly within the prefrontal and limbic networks that regulate emotion and behavior. Further studies are needed to identify how changes in brain structure and function mediate the effects of maternal obesity on long-term emotional and behavioral outcomes, as well as the molecular pathways through which maternal obesity alters offspring brain development.","PeriodicalId":16490,"journal":{"name":"Journal of Neuroscience Research","volume":"102 7","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jnr.25368","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Restoration of sleep–wake behavior following short photoperiod exposure in ventral subicular lesioned male Wistar rats: A 24-h sleep–wake electroencephalographical study 短光周期照射后雄性 Wistar 大鼠腹侧线粒体病变后睡眠-觉醒行为的恢复：24小时睡眠-觉醒脑电图研究

IF 2.9 3区医学

Journal of Neuroscience Research Pub Date : 2024-07-13 DOI: 10.1002/jnr.25367

Neethi Prem, Arun Sasidharan, Bettadapura N. Srikumar, Byrathnahalli S. Shankaranarayana Rao, Bindu M. Kutty

{"title":"Restoration of sleep–wake behavior following short photoperiod exposure in ventral subicular lesioned male Wistar rats: A 24-h sleep–wake electroencephalographical study","authors":"Neethi Prem, Arun Sasidharan, Bettadapura N. Srikumar, Byrathnahalli S. Shankaranarayana Rao, Bindu M. Kutty","doi":"10.1002/jnr.25367","DOIUrl":"10.1002/jnr.25367","url":null,"abstract":"The ventral subiculum regulates emotion, stress responses, and spatial and social cognition. In our previous studies, we have demonstrated anxiety- and depression-like symptoms, deficits in spatial and social cognition in ventral subicular lesioned (VSL) rats, and restoration of affective and cognitive behaviors following photoperiod manipulation (short photoperiod regime, SPR; 6:18 LD cycle). In the present study, we have studied the impact of VSL on sleep–wake behavioral patterns and the effect of SPR on sleep–wakefulness behavior. Adult male Wistar rats subjected to VSL demonstrated decreased wake duration and enhanced total sleep time due to increased non-rapid eye movement sleep (NREMS) and rapid eye movement sleep (REMS). Power spectral analysis indicated increased delta activity during NREMS and decreased sigma band power during all vigilance states. Light is one of the strongest entrainers of the circadian rhythm, and its manipulation may have various physiological and functional consequences. We investigated the effect of 21-day exposure to SPR on sleep–wakefulness (S–W) behavior in VSL rats. We observed that SPR exposure restored S–W behavior in VSL rats, resulting in an increase in wake duration and a significant increase in theta power during wake and REMS. This study highlights the crucial role of the ventral subiculum in maintaining normal sleep–wakefulness patterns and highlights the effectiveness of photoperiod manipulation as a non-pharmacological treatment for reversing sleep disturbances reported in mood and neuropsychiatric disorders like Alzheimer's disease, bipolar disorder, and major depressive disorder, which also involve alterations in circadian rhythm.","PeriodicalId":16490,"journal":{"name":"Journal of Neuroscience Research","volume":"102 7","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141600209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A strike to the head: Parallels between the pediatric and adult human and the rodent in traumatic brain injury 头部受到撞击小儿、成人和啮齿动物在脑外伤中的相似之处。

IF 2.9 3区医学

Journal of Neuroscience Research Pub Date : 2024-07-02 DOI: 10.1002/jnr.25364

Allie M. Smith, Bernadette E. Grayson

{"title":"A strike to the head: Parallels between the pediatric and adult human and the rodent in traumatic brain injury","authors":"Allie M. Smith, Bernadette E. Grayson","doi":"10.1002/jnr.25364","DOIUrl":"10.1002/jnr.25364","url":null,"abstract":"Traumatic brain injury (TBI) is a condition that occurs commonly in children from infancy through adolescence and is a global health concern. Pediatric TBI presents with a bimodal age distribution, with very young children (0–4 years) and adolescents (15–19 years) more commonly injured. Because children's brains are still developing, there is increased vulnerability to the effects of head trauma, which results in entirely different patterns of injury than in adults. Pediatric TBI has a profound and lasting impact on a child's development and quality of life, resulting in long-lasting consequences to physical, cognitive, and emotional development. Chronic issues like learning disabilities, behavioral problems, and emotional disturbances can develop. Early intervention and ongoing support are critical for minimizing these long-term deficits. Many animal models of TBI exist, and each varies significantly, displaying different characteristics of clinical TBI. The neurodevelopment differs in the rodent from the human in timing and effect, so TBI outcomes in the juvenile rodent can thus vary from the human child. The current review compares findings from preclinical TBI work in juvenile and adult rodents to clinical TBI research in pediatric and adult humans. We focus on the four brain regions most affected by TBI: the prefrontal cortex, corpus callosum, hippocampus, and hypothalamus. Each has its unique developmental projections and thus is impacted by TBI differently. This review aims to compare the healthy neurodevelopment of these four brain regions in humans to the developmental processes in rodents.","PeriodicalId":16490,"journal":{"name":"Journal of Neuroscience Research","volume":"102 7","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gray matter alterations in Huntington's disease: A meta-analysis of VBM neuroimaging studies 亨廷顿氏病的灰质改变：VBM神经影像学研究的荟萃分析。

IF 2.9 3区医学

Journal of Neuroscience Research Pub Date : 2024-07-02 DOI: 10.1002/jnr.25366

Xi Wang, Yuming Li, Boyi Li, Huifang Shang, Jing Yang

{"title":"Gray matter alterations in Huntington's disease: A meta-analysis of VBM neuroimaging studies","authors":"Xi Wang, Yuming Li, Boyi Li, Huifang Shang, Jing Yang","doi":"10.1002/jnr.25366","DOIUrl":"10.1002/jnr.25366","url":null,"abstract":"Increasing neuroimaging studies have attempted to identify biomarkers of Huntington's disease (HD) progression. Here, we conducted voxel-based meta-analyses of voxel-based morphometry (VBM) studies on HD to investigate the evolution of gray matter volume (GMV) alterations and explore the effects of genetic and clinical features on GMV changes. A systematic review was performed to identify the relevant studies. Meta-analyses of whole-brain VBM studies were performed to assess the regional GMV changes in all HD mutation carriers, in presymptomatic HD (pre-HD), and in symptomatic HD (sym-HD). A quantitative comparison was performed between pre-HD and sym-HD. Meta-regression analyses were used to explore the effects of genetic and clinical features on GMV changes. Twenty-eight studies were included, comparing a total of 1811 HD mutation carriers [including 1150 pre-HD and 560 sym-HD] and 969 healthy controls (HCs). Pre-HD showed decreased GMV in the bilateral caudate nuclei, putamen, insula, anterior cingulate/paracingulate gyri, middle temporal gyri, and left dorsolateral superior frontal gyrus compared with HCs. Compared with pre-HD, GMV decrease in sym-HD extended to the bilateral median cingulate/paracingulate gyri, Rolandic operculum and middle occipital gyri, left amygdala, and superior temporal gyrus. Meta-regression analyses found that age, mean lengths of CAG repeats, and disease burden were negatively associated with GMV atrophy of the bilateral caudate and right insula in all HD mutation carriers. This meta-analysis revealed the pattern of GMV changes from pre-HD to sym-HD, prompting the understanding of HD progression. The pattern of GMV changes may be biomarkers for disease progression in HD.","PeriodicalId":16490,"journal":{"name":"Journal of Neuroscience Research","volume":"102 7","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Newborn auditory brainstem response and sudden infant death syndrome 新生儿听觉脑干反应与婴儿猝死综合症

IF 4.2 3区医学

Journal of Neuroscience Research Pub Date : 2024-06-19 DOI: 10.1002/jnr.25362

Sarah E. Maylott, Guangyu Zeng, Tiffany S. Leung, Catalina Sanchez Montenegro, Alexia Barrios, Arushi Malik, Rafael E. Delgado, Christine F. Delgado, Elizabeth A. Simpson

{"title":"Newborn auditory brainstem response and sudden infant death syndrome","authors":"Sarah E. Maylott, Guangyu Zeng, Tiffany S. Leung, Catalina Sanchez Montenegro, Alexia Barrios, Arushi Malik, Rafael E. Delgado, Christine F. Delgado, Elizabeth A. Simpson","doi":"10.1002/jnr.25362","DOIUrl":"10.1002/jnr.25362","url":null,"abstract":"Sudden infant death syndrome (SIDS)—the sudden and unexplained death of a seemingly healthy infant, <1 year old—may be associated with abnormalities in the brain regions that underlie breathing and arousal during sleep. While post-mortem studies suggest abnormalities in SIDS infants' brainstems, there are no studies of these infants' brainstem function before death. One way to assess the function of the brainstem is with auditory brainstem response (ABR), a routine hearing-screening method that noninvasively measures the brainstem's response to sound. We hypothesize that anomalies in newborns' ABR measures may predict SIDS. Indeed, previous studies identified abnormalities in ABR characteristics in small samples of near-miss SIDS infants hospitalized for infant apnea syndrome. However, there is a need to examine the ABRs of infants who died of SIDS. Therefore, in the current study, we propose integrating two secondary datasets to examine newborns' ABRs (N = 156,972), including those who later died of SIDS (n = ~42; .27 out of every 1000 infants), using existing archived records of neonatal ABR results from a sample of newborns born in Florida. We hypothesize that infants who die from SIDS are more likely than non-SIDS infants to have abnormal ABRs as newborns. Understanding the association between SIDS and ABR may facilitate more accurate identification of an infant's risk for SIDS at birth, enabling increased monitoring, which may facilitate interventions and improve survivorship.","PeriodicalId":16490,"journal":{"name":"Journal of Neuroscience Research","volume":"102 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jnr.25362","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal summation of second pain is affected by cognitive load 第二次疼痛的时间总和受认知负荷的影响。

IF 4.2 3区医学

Journal of Neuroscience Research Pub Date : 2024-06-19 DOI: 10.1002/jnr.25363

Lara Rubal-Otero, Antonio Gil-Ugidos, Alberto Jacobo González Villar, María Teresa Carrillo-de-la-Peña

{"title":"Temporal summation of second pain is affected by cognitive load","authors":"Lara Rubal-Otero, Antonio Gil-Ugidos, Alberto Jacobo González Villar, María Teresa Carrillo-de-la-Peña","doi":"10.1002/jnr.25363","DOIUrl":"10.1002/jnr.25363","url":null,"abstract":"This work attempted to clarify the interaction of cognition and pain sensitization during a paradigm of Temporal Summation of Second Pain (TSSP). We analyzed pain ratings and electroencephalographic (EEG) activity obtained from 21 healthy participants during the presentation of four experimental conditions that differed in the manipulation of attention to painful stimuli or working memory load (Attention to hand & TSSP; 0-back & TSSP (low cognitive load); 2-back & TSSP (high cognitive load); 2-back (without pain)). We found that the TSSP was reduced when the attention was diverted and the cognitive load increased, and this reduction was accompanied by higher midfrontal theta activity and lower posterior alpha and central beta activity. Although it is well established that TSSP is a phenomenon that occurs at the spinal level, here we show that it is also affected by supraspinal attentional mechanisms. Delivery of painful repeated stimuli did not affect the performance of the 2-back task but was associated with smaller amplitudes of attentional event-related potentials (ERPs) after standard stimuli (not the target). The study of brain activity during TSSP allowed to clarify the role of top-down attentional modulation in pain sensitization processes. Results contribute to a better understanding of cognitive dysfunction in pain conditions and reinforce the use of therapeutic strategies based on distracting attention away from pain.","PeriodicalId":16490,"journal":{"name":"Journal of Neuroscience Research","volume":"102 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jnr.25363","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progresses in the establishment, evaluation, and application of in vitro blood–brain barrier models 体外血脑屏障模型的建立、评估和应用进展。

IF 4.2 3区医学

Journal of Neuroscience Research Pub Date : 2024-06-10 DOI: 10.1002/jnr.25359

Panfeng Yin, Xianchun Wang

引用次数: 0

Effects of a novel regimen of repetitive transcranial magnetic stimulation (rTMS) on neural remodeling and motor function in adult male mice with ischemic stroke 重复经颅磁刺激（rTMS）对缺血性中风成年雄性小鼠神经重塑和运动功能的影响。

IF 4.2 3区医学

Journal of Neuroscience Research Pub Date : 2024-06-10 DOI: 10.1002/jnr.25358

Wang Peipei, Deng Yu, Lin Xiaoyan, Liu Yunxia, Liang Liuming, Cheng Tongbin, Lv Shaoping

{"title":"Effects of a novel regimen of repetitive transcranial magnetic stimulation (rTMS) on neural remodeling and motor function in adult male mice with ischemic stroke","authors":"Wang Peipei, Deng Yu, Lin Xiaoyan, Liu Yunxia, Liang Liuming, Cheng Tongbin, Lv Shaoping","doi":"10.1002/jnr.25358","DOIUrl":"10.1002/jnr.25358","url":null,"abstract":"Neuroinflammation caused by excessive microglial activation plays a key role in the pathogenesis of ischemic stroke. Repetitive transcranial magnetic stimulation (rTMS) is a noninvasive neuromodulatory technique that has recently been reported to regulate microglial functions and exert anti-inflammatory effects. The intermittent burst stimulation (iTBS) regimen in rTMS improves neuronal excitability. However, whether iTBS exerts its anti-inflammatory effects by stimulating neurons and thereby modulating microglial polarization remains unclear. Motor function was assessed after 1 week of rTMS (iTBS regimen) treatment in adult male mice with occlusion/reperfusion of the middle cerebral artery (MCAO/r) injury. We also investigated the molecular biological alterations associated with microglial polarization using a cell proliferation assay, multiplex cytokine bioassays, and immunofluorescence staining. iTBS regimen can improve balance and motor coordination function, increase spontaneous movement, and improve walking function in mice with early cerebral ischemia injury. Expression levels of IL-1β, TNF-α, and IL-10 increased significantly in mice with MCAO injury. Especially, rTMS significantly increased the number of proliferating cells in the infarcted cortex. The fluorescence intensity of MAP2 in the peri-infarct area of MCAO injured mice was low, but the signal was broader. Compared with MCAO group, the fluorescence intensity of MAP2 in rTMS group was significantly increased. rTMS inhibited pro-inflammatory M1 activation (Iba1+/CD86+) and improved anti-inflammatory M2 activation (Iba1+/CD206+) in the peri-infarct zone, thus significantly changing the phenotypic ratio M1/M2. rTMS improves motor dysfunction and neuroinflammation after cerebral I/R injury in mice by regulating microglial polarization.","PeriodicalId":16490,"journal":{"name":"Journal of Neuroscience Research","volume":"102 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141300851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0