Journal of Neuroscience Methods最新文献

{"title":"Machine learning approaches to predict whether MEPs can be elicited via TMS","authors":"Fang Jin ,&nbsp;Sjoerd M. Bruijn ,&nbsp;Andreas Daffertshofer","doi":"10.1016/j.jneumeth.2024.110242","DOIUrl":"10.1016/j.jneumeth.2024.110242","url":null,"abstract":"<div><h3>Background</h3><p>Transcranial magnetic stimulation (TMS) is a valuable technique for assessing the function of the motor cortex and cortico-muscular pathways. TMS activates the motoneurons in the cortex, which after transmission along cortico-muscular pathways can be measured as motor-evoked potentials (MEPs). The position and orientation of the TMS coil and the intensity used to deliver a TMS pulse are considered central TMS setup parameters influencing the presence/absence of MEPs.</p></div><div><h3>New method</h3><p>We sought to predict the presence of MEPs from TMS setup parameters using machine learning. We trained different machine learners using either within-subject or between-subject designs.</p></div><div><h3>Results</h3><p>We obtained prediction accuracies of on average 77 % and 65 % with maxima up to up to 90 % and 72 % within and between subjects, respectively. Across the board, a bagging ensemble appeared to be the most suitable approach to predict the presence of MEPs.</p></div><div><h3>Conclusions</h3><p>Although within a subject the prediction of MEPs via TMS setup parameter-based machine learning might be feasible, the limited accuracy between subjects suggests that the transfer of this approach to experimental or clinical research comes with significant challenges.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"410 ","pages":"Article 110242"},"PeriodicalIF":2.7,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165027024001870/pdfft?md5=66a40e55edad1daf20c42544f140f858&pid=1-s2.0-S0165027024001870-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of neural function recovery in premature infants at high risk of brain injury using amplitude integrated electroencephalography and GMs scales","authors":"Chun Lin,&nbsp;Bo Chen,&nbsp;Zhiqiang Wang,&nbsp;Andi Zou,&nbsp;Minghui Ke","doi":"10.1016/j.jneumeth.2024.110246","DOIUrl":"10.1016/j.jneumeth.2024.110246","url":null,"abstract":"<div><p>Preterm infants are a high-risk group for brain injury, and it is important to evaluate the neurological recovery of preterm infants. Therefore, this paper evaluates the neurological recovery in preterm infants at high risk of brain injury by amplitude-integrated EEG and GMs scale. The study collected basic information on preterm infants and performed amplitude integrated EEG examination and GMs scale evaluation. Amplitude integrated EEG examination attaches electrodes using multielectrode arrays onto specific areas of the premature head to record brain wave activity to monitor electrical activity in the preterm brain in real time and amplify and process through the signals received by the electrodes to obtain more detailed EEG data. The GMs scale evaluates the developmental and functional status of the child and allows an objective assessment of the development and recovery of neurological function by observing their performance in motor, language, cognition, and social interaction. Analysis of the data by statistical processing. The results showed that early brain injury was evident in high-risk infants. Amplitude integrated EEG parameters can have some predictive value for brain injury. There were also differences in GMs scale assessment between brain injury and non-brain injury. Amplitude integrated EEG combined with GMs scale has certain value in predicting brain injury and can provide an important basis for early intervention in children with preterm brain injury and help to improve their neurodevelopmental outcome.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"410 ","pages":"Article 110246"},"PeriodicalIF":2.7,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CircadiPy: An open-source toolkit for analyzing chronobiology time series","authors":"João Pedro Carvalho-Moreira ,&nbsp;Leonardo de Oliveira Guarnieri ,&nbsp;Matheus Costa Passos ,&nbsp;Felipe Emrich ,&nbsp;Paula Bargi-Souza ,&nbsp;Rodrigo Antonio Peliciari-Garcia ,&nbsp;Márcio Flávio Dutra Moraes","doi":"10.1016/j.jneumeth.2024.110245","DOIUrl":"10.1016/j.jneumeth.2024.110245","url":null,"abstract":"<div><h3>Background</h3><p>Chronobiology is the scientific field focused on studying periodicity in biological processes. In mammals, most physiological variables exhibit circadian rhythmicity, such as metabolism, body temperature, locomotor activity, and sleep. The biological rhythmicity can be statistically evaluated by examining the time series and extracting parameters that correlate to the period of oscillation, its amplitude, phase displacement, and overall variability.</p></div><div><h3>New method</h3><p>We have developed a library called CircadiPy, which encapsulates methods for chronobiological analysis and data inspection, serving as an open-access toolkit for the analysis and interpretation of chronobiological data. The package was designed to be flexible, comprehensive and scalable in order to assist research dealing with processes affected or influenced by rhythmicity.</p></div><div><h3>Results</h3><p>The results demonstrate the toolkit's capability to guide users in analyzing chronobiological data collected from various recording sources, while also providing precise parameters related to the circadian rhythmicity.</p></div><div><h3>Comparison with existing methods</h3><p>The analysis methodology from this proposed library offers an opportunity to inspect and obtain chronobiological parameters in a straightforward and cost-free manner, in contrast to commercial tools.</p></div><div><h3>Conclusions</h3><p>Moreover, being an open-source tool, it empowers the community with the opportunity to contribute with new functions, analysis methods, and graphical visualizations given the simplified computational method of time series data analysis using an easy and comprehensive pipeline within a single Python object.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"411 ","pages":"Article 110245"},"PeriodicalIF":2.7,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A robust paradigm for studying regeneration after traumatic spinal cord injury in zebrafish","authors":"Gentry Andrews ,&nbsp;Geoffrey Andrews ,&nbsp;Yuk Fai Leung ,&nbsp;Daniel M. Suter","doi":"10.1016/j.jneumeth.2024.110243","DOIUrl":"10.1016/j.jneumeth.2024.110243","url":null,"abstract":"<div><h3>Background</h3><p>Zebrafish are vertebrates with a high potential of regeneration after injury in the central nervous system. Therefore, they have emerged as a useful model system for studying traumatic spinal cord injuries.</p></div><div><h3>New Method</h3><p>Using larval zebrafish, we have developed a robust paradigm to model the effects of anterior spinal cord injury, which correspond to the debilitating injuries of the cervical and thoracic regions in humans. Our new paradigm consists of a more anterior injury location compared to previous studies, a modified behavioral assessment using the visual motor response, and a new data analysis code.</p></div><div><h3>Results</h3><p>Our approach enables a spinal cord injury closer to the hindbrain with more functional impact compared to previous studies using a more posterior injury location. Results reported in this work reveal recovery over seven days following spinal cord injury.</p></div><div><h3>Comparing with existing Methods</h3><p>The present work describes a modified paradigm for the <em>in vivo</em> study of spinal cord regeneration after injury using larval zebrafish, including an anterior injury location, a robust behavioral assessment, and a new data analysis software.</p></div><div><h3>Conclusions</h3><p>Our findings lay the foundation for applying this paradigm to study the effects of drugs, nutrition, and other treatments to improve the regeneration process.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"410 ","pages":"Article 110243"},"PeriodicalIF":2.7,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An automated planar patch-clamp approach to measure the membrane potential and resting membrane currents in a human cerebrovascular endothelial cell line","authors":"Teresa Soda ,&nbsp;Sharon Negri ,&nbsp;Giorgia Scarpellino ,&nbsp;Roberto Berra-Romani ,&nbsp;Giovambattista De Sarro ,&nbsp;Francesco Moccia ,&nbsp;Valentina Brunetti","doi":"10.1016/j.jneumeth.2024.110248","DOIUrl":"10.1016/j.jneumeth.2024.110248","url":null,"abstract":"<div><h3>Background</h3><p>The conventional “whole-cell patch-clamp” recording technique is widely used to measure the resting membrane potential (V_M) and to dissect the underlying membrane ionic conductances in isolated vascular endothelial cells.</p></div><div><h3>New method</h3><p>Herein, we assessed whether the automated patch-clamp (APC) technology, which replaces the traditional patch-pipette with a planar substrate to permit researchers lacking formal training in electrophysiology to generate large amounts of data in a relatively short time, can be used to characterize the bioelectrical activity of vascular endothelial cells. We assessed whether the Port-a-Patch planar patch-clamp system, which is regarded as the smallest electrophysiological rig available on the market, can be used to measure the V_M and resting membrane currents in the human cerebrovascular endothelial cell line, hCMEC/D3.</p></div><div><h3>Comparison with existing methods</h3><p>We demonstrated that the Port-a-Patch planar patch-clamp system provides the same values of the resting V_M as those provided by the conventional patch-clamp technique. Furthermore, the APC technology provides preliminary data demonstrating that the resting V_M of hCMEC/D3 cells is primarily contributed by Cl^- and Na⁺, as demonstrated with the patch-clamp technique for many other endothelial cell types.</p></div><div><h3>Conclusions</h3><p>The Port-a-Patch planar patch-clamp system can be successfully used to measure the resting V_M and the underlying membrane ionic conductances in hCMEC/D3 cells. We envisage that this easy-to-use APC system could also be extremely useful for the investigation of the membrane currents that can be activated by chemical, thermal, optical, and mechanical stimuli in this cell line as well as in other types of isolated vascular endothelial cells.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"410 ","pages":"Article 110248"},"PeriodicalIF":2.7,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test-retest reliability and normative data for “Seven-iTT”, a test for the assessment of taste and oral trigeminal function","authors":"Mariano Mastinu ,&nbsp;Andreas Püschner ,&nbsp;Saskia Gerlach,&nbsp;Thomas Hummel","doi":"10.1016/j.jneumeth.2024.110244","DOIUrl":"10.1016/j.jneumeth.2024.110244","url":null,"abstract":"<div><h3>Background</h3><p>Assessment of taste and somatosensory perception in clinical practice lacks fast tests that are validated and reliable. Recently, a 12-item identification test for taste and oral trigeminal perception, and its shorter version, the Seven-iTT, was developed. The objectives of this study were to evaluate its test-retest reliability and establish normative data.</p></div><div><h3>New method</h3><p>Two-hundred participants (120 women, 80 men) with a good sense of taste performed a whole-mouth identification test using 12 filter-paper strips impregnated with low and high concentrations of sweet, sour, salty, bitter, astringency, and spiciness. Fifty of them repeated the task, with a median interval of 122 days from the first visit. Test-retest reliability was determined using Spearman correlation and the Bland–Altman plot method.</p></div><div><h3>Results</h3><p>There was a significant correlation in identification score between the first and the second session for both versions of the test (r ≥ 0.28; <em>p</em> ≤ 0.048). The Bland–Altman plot reflected a good congruence between the results of the two sessions. Additionally, frequencies of correct identification were consistent between sessions, with women outperforming men (<em>p</em> = 0.005). Hypogeusia was established at Seven-iTT score of 3 of less.</p></div><div><h3>Comparison with existing methods</h3><p>The identification test combines taste and somatosensory perception, thus creating a more detailed diagnosis tool. Scores were correlated with self-rated taste perception.</p></div><div><h3>Conclusion</h3><p>The present results confirmed the applicability of Seven-iTT for a reliable, fast evaluation of taste and somatosensory perception in the general population, that can be extended to clinical practice.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"410 ","pages":"Article 110244"},"PeriodicalIF":2.7,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165027024001894/pdfft?md5=5df0e8b79bfb5b9fc51615662e9a83d1&pid=1-s2.0-S0165027024001894-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human-robot interaction in motor imagery: A system based on the STFCN for unilateral upper limb rehabilitation assistance","authors":"Hui Tian","doi":"10.1016/j.jneumeth.2024.110240","DOIUrl":"10.1016/j.jneumeth.2024.110240","url":null,"abstract":"<div><h3>Background</h3><p>Rehabilitation training based on the brain-computer interface of motor imagery (MI-BCI) can help restore the connection between the brain and movement. However, the performance of most popular MI-BCI system is coarse-level, which means that they are good at guiding the rehabilitation exercises of different parts of the body, but not for the individual component.</p></div><div><h3>New methods</h3><p>In this paper, we designed a fine-level MI-BCI system for unilateral upper limb rehabilitation assistance. Besides, due to the low discrimination of different sample classes in a single part, a classification algorithm called spatial-temporal filtering convolutional network (STFCN) was proposed that used spatial filtering and deep learning.</p></div><div><h3>Comparison with existing methods</h3><p>Our STFCN outperforms popular methods in recent years using BCI IV 2a and 2b data sets.</p></div><div><h3>Results</h3><p>To verify the effectiveness of our system, we recruited 6 volunteers and collected their data for a four-classification online experiments, resulting in an average accuracy of 62.7 %.</p></div><div><h3>Conclusion</h3><p>This fine-level MI-BCI system has good appli-cation prospects, and inspires more exploration of rehabilitation in a single part of the human body.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"411 ","pages":"Article 110240"},"PeriodicalIF":2.7,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EEG Analyses of visual cue effects on executed movements","authors":"Patrick Suwandjieff ,&nbsp;Gernot R. Müller-Putz","doi":"10.1016/j.jneumeth.2024.110241","DOIUrl":"10.1016/j.jneumeth.2024.110241","url":null,"abstract":"<div><h3>Background</h3><p>In electroencephalographic (EEG) or electrocorticographic (ECoG) experiments, visual cues are commonly used for timing synchronization but may inadvertently induce neural activity and cognitive processing, posing challenges when decoding self-initiated tasks.</p></div><div><h3>New method</h3><p>To address this concern, we introduced four new visual cues (Fade, Rotation, Reference, and Star) and investigated their impact on brain signals. Our objective was to identify a cue that minimizes its influence on brain activity, facilitating cue-effect free classifier training for asynchronous applications, particularly aiding individuals with severe paralysis.</p></div><div><h3>Results</h3><p>22 able-bodied, right-handed participants aged 18–30 performed hand movements upon presentation of the visual cues. Analysis of time-variability between movement onset and cue-aligned data, grand average MRCP, and classification outcomes revealed significant differences among cues. Rotation and Reference cue exhibited favorable results in minimizing temporal variability, maintaining MRCP patterns, and achieving comparable accuracy to self-paced signals in classification.</p></div><div><h3>Comparison with existing methods</h3><p>Our study contrasts with traditional cue-based paradigms by introducing novel visual cues designed to mitigate unintended neural activity. We demonstrate the effectiveness of Rotation and Reference cue in eliciting consistent and accurate MRCPs during motor tasks, surpassing previous methods in achieving precise timing and high discriminability for classifier training.</p></div><div><h3>Conclusions</h3><p>Precision in cue timing is crucial for training classifiers, where both Rotation and Reference cue demonstrate minimal variability and high discriminability, highlighting their potential for accurate classifications in online scenarios. These findings offer promising avenues for refining brain-computer interface systems, particularly for individuals with motor impairments, by enabling more reliable and intuitive control mechanisms.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"410 ","pages":"Article 110241"},"PeriodicalIF":2.7,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165027024001869/pdfft?md5=5731759521f08f900566c2aa88198bbd&pid=1-s2.0-S0165027024001869-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of cerebrospinal fluid (CSF) and interstitial fluid (ISF) mouse proteomes for the validation and description of Alzheimer’s disease biomarkers","authors":"Anna Maria Górska ,&nbsp;Irene Santos-García ,&nbsp;Ivan Eiriz ,&nbsp;Thomas Brüning ,&nbsp;Tuula Nyman ,&nbsp;Jens Pahnke","doi":"10.1016/j.jneumeth.2024.110239","DOIUrl":"10.1016/j.jneumeth.2024.110239","url":null,"abstract":"<div><h3>Background</h3><p>Mass spectrometry (MS)-based cerebrospinal fluid (CSF) proteomics is an important method for discovering biomarkers of neurodegenerative diseases. CSF serves as a reservoir for interstitial fluid (ISF), and extensive communication between the two fluid compartments helps to remove waste products from the brain.</p></div><div><h3>New method</h3><p>We performed proteomic analyses of both CSF and ISF fluid compartments using intracerebral microdialysis to validate and detect novel biomarkers of Alzheimer's disease (AD) in APPtg and C57Bl/6J control mice.</p></div><div><h3>Results</h3><p>We identified up to 625 proteins in ISF and 4483 proteins in CSF samples. By comparing the biofluid profiles of APPtg and C57Bl/6J mice, we detected 37 and 108 significantly up- and downregulated candidates, respectively. In ISF, 7 highly regulated proteins, such as Gfap, Aldh1l1, Gstm1, and Txn, have already been implicated in AD progression, whereas in CSF, 9 out of 14 highly regulated proteins, such as Apba2, Syt12, Pgs1 and Vsnl1, have also been validated to be involved in AD pathogenesis. In addition, we also detected new interesting regulated proteins related to the control of synapses and neurotransmission (Kcna2, Cacng3, and Clcn6) whose roles as AD biomarkers should be further investigated.</p></div><div><h3>Comparison with existing methods</h3><p>This newly established combined protocol provides better insight into the mutual communication between ISF and CSF as an analysis of tissue or CSF compartments alone.</p></div><div><h3>Conclusions</h3><p>The use of multiple fluid compartments, ISF and CSF, for the detection of their biological communication enables better detection of new promising AD biomarkers.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"411 ","pages":"Article 110239"},"PeriodicalIF":2.7,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165027024001845/pdfft?md5=f2c05794bf6ed72e7ad396accb4086a3&pid=1-s2.0-S0165027024001845-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and customizability: Expanding possibilities with 3D printed guide cannulas","authors":"Rogneda B. Kazanskaya ,&nbsp;Anna N. Berliand ,&nbsp;Anna B. Volnova ,&nbsp;Alexander V. Lopachev","doi":"10.1016/j.jneumeth.2024.110237","DOIUrl":"10.1016/j.jneumeth.2024.110237","url":null,"abstract":"<div><h3>Background</h3><p>Intracerebral cannulation bypasses the blood-brain barrier, and is frequently used for targeted drug delivery to specific brain structures. Despite the availability of brain infusion kits and manual injections without cannulation, the traditional design of guide cannulas continues to be utilized in research. Several protocols describing guide cannula manufacture from stainless steel needles have been published previously.</p></div><div><h3>New method</h3><p>We describe a method for producing the first fully plastic guide cannula intended for intracerebroventricular injections in mice using Dental Sand A1-A2 resin and digital light processing 3D printing.</p></div><div><h3>Results</h3><p>The lack of resin neurotoxicity for primary rat cortical neuron cultures was shown. Histological evaluations performed 6 weeks after guide cannula implantation to C57/black mice show that plastic cannula are biocompatible. Microglial and astroglial reactions to plastic cannulas are reduced compared to lab-made stainless steel cannulas. Plastic cannulas are less prone to obstruction, and remained unobstructed over the course of 3 weeks of daily injections, while 50 % of stainless steel cannula became impassable by the 2 week mark.</p></div><div><h3>Comparison with existing methods</h3><p>These are the first published cannulas intended for applications in mice which combine the presence of usable threads, allowing dummy cannula fixation, with a low profile and small footprint compared to commercially available cannulas.</p></div><div><h3>Conclusions</h3><p>Editable parametric and stl files for reproducing the cannulas presented in this manuscript are included. The method described in this paper is accessible to most laboratories, enabling near-perfect standardization in length combined with a high level of customizability.</p></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"410 ","pages":"Article 110237"},"PeriodicalIF":2.7,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}