Journal of microbiological methods最新文献

{"title":"Pancreatic stone protein levels accurately predict severity in sepsis of various causes earlier than other biomarkers","authors":"Dimitrios Kouroupis ,&nbsp;Charalampos Zarras ,&nbsp;Theocharis Koufakis ,&nbsp;Maria Terzaki ,&nbsp;Panagiotis Pateinakis ,&nbsp;Aristeidis Chalvantzis ,&nbsp;Konstantina Mpani ,&nbsp;Anthi Issa ,&nbsp;Prodromos Soukiouroglou ,&nbsp;Anastasia Sarvani ,&nbsp;Athina Pyrpasopoulou ,&nbsp;Michail Doumas ,&nbsp;Eleni Vagdatli","doi":"10.1016/j.mimet.2025.107129","DOIUrl":"10.1016/j.mimet.2025.107129","url":null,"abstract":"<div><div>The aim of this study was to compare the prognostic value of PSP in different types of sepsis, with common biomarkers. PSP levels were higher in bacteremia and Gram-negative sepsis and correlated with worse outcome (<em>p</em> = 0,006) earlier than WBC, CRP and PCT. It may aid for risk stratification in sepsis.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107129"},"PeriodicalIF":1.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subtractive genomics approach: A guide to unveiling therapeutic targets across pathogens","authors":"Bhavya Mudgal ,&nbsp;Devvret Verma ,&nbsp;Divya Venogopal ,&nbsp;Suraj V. Atram ,&nbsp;Debasis Mitra ,&nbsp;Sugam Gupta","doi":"10.1016/j.mimet.2025.107127","DOIUrl":"10.1016/j.mimet.2025.107127","url":null,"abstract":"<div><div>Subtractive genomics is an adaptable bioinformatics technique that is used to identify potential therapeutic targets by differentiating essential genes in pathogens and non-pathogenic genes. Since, identification of therapeutic targets and understanding of their structure, function, and role in pathogenesis is important in development of drug design. Therefore, this review will provide a comprehensive look at the subtractive genomics technique which was applied to various pathogens, often highlighting the effectiveness of the methodology in drug target discovery and novel therapeutics development. Tools and software such as BLAST, Roary, and AutoDock Vina are widely utilized in this methodology for various aspects such as, genome comparison, essential gene identification, clustering, subcellular localization, pathway analysis, molecular docking <em>etc.</em> Diseases such as tuberculosis, botulism, staphylococcal infections, ventilator-associated pneumonia, secondary meningitis, gonorrhoea, septicaemia, <em>etc.,</em> are among the infectious diseases targeted using subtractive genomics. Comparison of basic principles, tools, and advancements use these subtractive genomics studies, will provide insight into the adaptable nature of this technique and the diversity of pathogens, which have benefited with this methodology into providing successful results. The main focus is on the genome sequencing advancements, annotation and validation through <em>in-</em>silico techniques, to find effective drug targets while, decreasing the possibility of toxicity in the host. We have also discussed the possibility of taking a multi-omics approach and incorporating AI and machine learning to expand on the current data and finding effective therapeutics for helping globally on health challenges.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107127"},"PeriodicalIF":1.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of saliva collected by the Self-Lollisponge® device for diagnosis of malaria in children","authors":"Enoch Aninagyei ,&nbsp;Comfort Addo Boatey ,&nbsp;Joshua Nkunim Arthur-Asmah ,&nbsp;Gifty Larbi ,&nbsp;Benjamin Sarfo-Bempong ,&nbsp;Wilson Bright Tsidi ,&nbsp;Charity Asantewaa Ankomah ,&nbsp;Regina Yayra Menu ,&nbsp;Keren Trishia Yemofio ,&nbsp;Desmond Omane Acheampong","doi":"10.1016/j.mimet.2025.107128","DOIUrl":"10.1016/j.mimet.2025.107128","url":null,"abstract":"<div><div>The Self-Lollisponge® device was used to collect saliva in children with malaria. Malaria antigens, namely, <em>P. falciparum</em> histidine-rich proteins 2 (PfHRP2) and <em>Plasmodium</em> lactate dehydrogenase (pLDH) were detected in blood and saliva samples. Subsequently, replicate samples with malaria antigens were stored at room temperature (28 °C) and 6 °C for 7 days. Malaria antigens were detected on days 1, 3, and 7. In the blood, PfHRP2 was detected in all 212 malaria samples, while pLDH was detected in 117 of the 212 samples (55.2 %). In the saliva, PfHRP2 was detected in 99/212 (46.7 %) samples while pLDH was detected in 111 of the 117 (94.8 %) samples whose corresponding blood had the pLDH detected. Compared to the blood samples, the overall sensitivity and accuracy of using saliva to detect PfHRP2 antigens were 46.7 % (95 % CI: 39.8–53.7 %) and 56.9 % (95 % CI: 50.6–63 %), respectively. Further, the overall sensitivity and accuracy for the detection of saliva-pLDH were 94.8 % (95 % CI: 89.2–98.1 %) and 96.4 % (95 % CI: 92.3–98.7 %), respectively. The PfHRP2 antigens were preserved for three days at 6 °C but at room temperature, the stability was for just a day. Nonetheless, the pLDH reduced comparatively faster, irrespective of the storage temperature.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107128"},"PeriodicalIF":1.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical microbiology and artificial intelligence: Different applications, challenges, and future prospects","authors":"Wafaa S. Khalaf ,&nbsp;Radwa N. Morgan ,&nbsp;Walid F. Elkhatib","doi":"10.1016/j.mimet.2025.107125","DOIUrl":"10.1016/j.mimet.2025.107125","url":null,"abstract":"<div><div>Conventional clinical microbiological techniques are enhanced by the introduction of artificial intelligence (AI). Comprehensive data processing and analysis enabled the development of curated datasets that has been effectively used in training different AI algorithms. Recently, a number of machine learning (ML) and deep learning (DL) algorithms are developed and evaluated using diverse microbiological datasets. These datasets included spectral analysis (Raman and MALDI-TOF spectroscopy), microscopic images (Gram and acid fast stains), and genomic and protein sequences (whole genome sequencing (WGS) and protein data banks (PDBs)). The primary objective of these algorithms is to minimize the time, effort, and expenses linked to conventional analytical methods. Furthermore, AI algorithms are incorporated with quantitative structure-activity relationship (QSAR) models to predict novel antimicrobial agents that address the continuing surge of antimicrobial resistance. During the COVID-19 pandemic, AI algorithms played a crucial role in vaccine developments and the discovery of new antiviral agents, and introduced potential drug candidates via drug repurposing. However, despite their significant benefits, the implementation of AI encounters various challenges, including ethical considerations, the potential for bias, and errors related to data training. This review seeks to provide an overview of the most recent applications of artificial intelligence in clinical microbiology, with the intention of educating a wider audience of clinical practitioners regarding the current uses of machine learning algorithms and encouraging their implementation. Furthermore, it will discuss the challenges related to the incorporation of AI into clinical microbiology laboratories and examine future opportunities for AI within the realm of infectious disease epidemiology.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107125"},"PeriodicalIF":1.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel anti-C6 based approach for IgG avidity testing in Lyme borreliosis: A proof-of-concept study","authors":"András Zóka,&nbsp;Márton Gönczi,&nbsp;Róbert Steinhauser,&nbsp;Zsófia Ódor,&nbsp;Gabriella Bekő,&nbsp;Attila Bezzegh","doi":"10.1016/j.mimet.2025.107126","DOIUrl":"10.1016/j.mimet.2025.107126","url":null,"abstract":"<div><div>Differentiating past from ongoing infection by serology is challenging. The sequential antibody response enables the detection of seroprogression on line immunoassays (LIA) but the different immunological age of antibodies, hence their different stage of affinity maturation also hinders IgG avidity determination. The conserved C6 segment of VlsE evokes an early, robust IgG response, thus might prove a suitable antigen for avidity measurements. Sera from 49 patients were tested (6 erythema migrans - EM, 22 post-primary – PP and 21 past infection). Paired sera were available from 24 patients in whom the diagnosis was confirmed by seroprogression and/or intrathecal antibody production (6 EM, 7 PP and 11 past infection). An in-house C6 avidity ELISA was used with 6 M urea solution and avidity-enhanced LIAs for representative samples. The avidity of reactive early and late antibodies differed on avidity-enhanced LIAs. Baseline C6 IgG intensity was higher in the PP group (EM vs. PP vs. past medians: 0.36 [IQR: 0.3–0.44] vs. 3.64 [IQR: 2.7–4.2] vs. 0.58 [0.36–1.1], <em>p</em> &lt; 0.01, Kruskal-Wallis). C6 avidity at baseline did not differ in PP and past Lyme. Anti-C6 intensity and avidity evolved differently in the three groups between baseline and follow-up: in EM patients the avidity remained similarly low but the intensity increased (0.36 [0.3–0.44]) vs. 0.73 [0.62–0.87], <em>p</em> = 0.03, Wilcoxon); in PP Lyme the avidity increased (63.9 [42.1–73.6] vs. 74.7 [70–90.8], p = 0.03, Wilcoxon). Both the anti-C6 IgG avidity and intensity remained similar with residual antibodies from past infection. In conclusion, anti-C6 intensity and avidity changed as expected according to fundamental immunology. Anti-C6 IgG avidity testing might provide us with a useful, quantitative, supplementary tool in the future to differentiate past from active infection.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107126"},"PeriodicalIF":1.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of molecular and immunological based diagnostic tools to detect Orientia tsutsugamushi (scrub typhus) infection in human","authors":"Rajkumar Venkatesan ,&nbsp;Taju Gani ,&nbsp;Abdul Majeed Seepoo ,&nbsp;Philip Samuel Paulraj ,&nbsp;Govindarajan Renu ,&nbsp;Gowri Sankar Subbiah ,&nbsp;Vijesh Sreedhar Kuttiatt ,&nbsp;Nafeez Ahmed Abdul ,&nbsp;Suryakodi Selvam ,&nbsp;Abdul Wazith Mohamed Jaffer ,&nbsp;Kanimozhi Kumarasamy ,&nbsp;Vimal Sugumar ,&nbsp;Sahul Hameed Azeez Sait","doi":"10.1016/j.mimet.2025.107124","DOIUrl":"10.1016/j.mimet.2025.107124","url":null,"abstract":"<div><div>Scrub typhus is a disease caused by a bacterial pathogen, <em>Orientia tsutsugamushi</em> which spreads through bites of infected chigger mite. Fever, headache, body aches and rash are common symptoms of scrub typhus. The absence of specific signs and symptoms of the disease makes diagnosis extremely difficult. In the present study, the blood samples from individuals with infections were obtained and analyzed for IgM and IgG antibodies using a commercial ELISA test kit. The present work was carried out to develop a molecular and immunological based technique which are more sensitive and accurate for the early detection of infected persons. The molecular diagnostics like PCR and nested PCR used for amplification of the 56 kDa type specific antigen (TSA) are more specific, sensitive and accurate. The PCR amplified partial 56 kDa TSA gene was cloned into the vector pET27b (+), and expressed in the BL21 host. The recombinant protein was purified by Ni-NTA column, and then the purified protein was used for production of polyclonal antibody in mice and rabbits. The antibody was used to develop immunological based diagnostic tools like ELISA, Western blot and dot blot for the early detection of scrub typhus infection. Totally, 141 infected samples were screened by PCR, nPCR, ELISA and western blot developed in the present study and the results were compared to commercial ELISA kit. Out of 141 samples analyzed, 58.8 % (83 samples) tested positive by PCR, 73 % (103 samples) by nPCR, and 86.5 % (122 samples) by ELISA. Western blot analysis of the 14 selected positive samples showed positive results (100 %). The newly developed immunological diagnostic tools and PCR techniques demonstrated high sensitivity and enhanced specificity, allowing for early detection of scrub typhus infection.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107124"},"PeriodicalIF":1.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of limit of detection and relative limit of detection of Salmonella in raw pet food matrices using Salmonella bacteriological analytical manual methods","authors":"Shannon Kiener ,&nbsp;Emily Smith ,&nbsp;Neha Singh ,&nbsp;Sarah M. Nemser ,&nbsp;Karina Hettwer ,&nbsp;Megan R. Miller ,&nbsp;Andriy Tkachenko ,&nbsp;Steffen Uhlig ,&nbsp;Ravinder Reddy","doi":"10.1016/j.mimet.2025.107116","DOIUrl":"10.1016/j.mimet.2025.107116","url":null,"abstract":"<div><div>Each year, there are multiple <em>Salmonella</em> recalls due to contaminated pet food products. Routine testing of finished products requires reliable, quick, and sensitive detection methods. In this study we comparatively evaluated the sensitivity of a culture-based reference method and two alternative methods, which are based on LAMP and PCR principles. The evaluation was performed in multiple trials using challenging pet food matrices, which were tested after various storage time intervals to better address the reliability of the methods. Raw freeze-dried treats, kibble, and patties were individually inoculated with <em>Salmonella Typhimurium</em> at fractional and high inoculation levels. Raw freeze-dried treats and kibble were inoculated at 0, 1, 3, 5, 7 and 10 CFU/25 g and raw patties were inoculated at 0, 1, 3, 5, 7, 10 and 30 CFU/25 g. For the culture method, the level of detection (LOD₅₀) values were 0.9, 1.1, and 3.7 CFU/25 g for freeze-dried treats, kibble, and patties respectively. The calculated relative level of detection (RLOD) values were close to 1 CFU/25 g, indicating that LAMP and PCR methods have similar LOD₅₀ values to the culture method. It was also confirmed that the alternative LAMP and PCR methods can provide reliable results within 24 h that match the reference culture method. Faster response times are invaluable when responding to potential pet food product contamination.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107116"},"PeriodicalIF":1.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development of a subunit vaccine for Mycobacterium tuberculosis Rv0081 as a booster for BCG and the investigation of its immunogenicity","authors":"Yun Xu ,&nbsp;Qiangsen Zhong ,&nbsp;Xiaochun Wang ,&nbsp;Xinkuang Liu ,&nbsp;Zian Zhang ,&nbsp;LingYun Kong ,&nbsp;Mingming Zhou ,&nbsp;Runlin Wang","doi":"10.1016/j.mimet.2025.107121","DOIUrl":"10.1016/j.mimet.2025.107121","url":null,"abstract":"<div><div>Tuberculosis (TB) is the number one infectious disease killer worldwide. Bacillus Calmette-Guérin (BCG) is the only vaccine currently used in clinical practice to prevent TB, but it still has limitations in preventing latent infection and reactivation of TB. The investigation focused on the selection of the <em>Rv0081</em> antigen linked to latency, leading to the successful creation of the prokaryotic expression recombinant plasmid pET30b-Rv0081 for subsequent expression and purification processes. Antigen specificity was additionally validated through the implementation of whole blood IFN-γ release assay (WBIA), enzyme-linked immunosorbent assay (ELISA), and flow cytometry analysis. rRv0081 induced individuals infected with <em>Mycobacterium tuberculosis</em> (<em>M. tb</em>) to exhibit markedly elevated levels of IFN-γ in their peripheral blood compared to the control group of healthy individuals. Mice were co-immunized with rRv0081 and DMT, and serum specific antibodies, levels of cytokines secreted by splenocytes and the number of multifunctional T cells in splenocytes were detected. The study findings indicated a notable elevation in the secretion levels of IFN-γ, TNF-α, and IL-2 cytokines from splenocytes, along with increased levels of IgG and its subclasses in the serum of mice belonging to the BCG + rRv0081/DMT group compared to its control group. Furthermore, there was a significant increase in the count of single-positive and double-positive CD4⁺ and CD8⁺ T cells stimulated to generate IFN-γ and TNF-α in the BCG + rRv0081/DMT group when contrasted with the BCG group. This suggests that rRv0081/DMT could be a potential subunit vaccine candidate that may serve as an effective booster vaccine after primary immunization against BCG.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107121"},"PeriodicalIF":1.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorocarbon solvent scavenges indole and promotes Escherichia coli growth","authors":"Ryosuke Hosoki ,&nbsp;Katsuaki Izawa ,&nbsp;Kosuke Kawanaka ,&nbsp;Yoshihisa Yamashige ,&nbsp;Shojiro Kikuchi ,&nbsp;Yuichi Ogawa ,&nbsp;Masahiko Harata","doi":"10.1016/j.mimet.2025.107123","DOIUrl":"10.1016/j.mimet.2025.107123","url":null,"abstract":"<div><div>Fluorocarbon solvents have been shown to promote bacterial growth; however, the underlying mechanisms remain unclear. Here, we show that these solvents scavenge bacterially produced indole, reducing its inhibitory effect on growth. These findings can be utilized to improve bacterial culture methods and explore the roles of indole in bacterial communities.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107123"},"PeriodicalIF":1.7,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotyping of Corynebacterium pseudotuberculosis isolates using PCR-based DNA fingerprinting methods","authors":"Şeyda Yaman ,&nbsp;Çağatay Nuhay ,&nbsp;Arzu Fındık ,&nbsp;Alper Çiftci","doi":"10.1016/j.mimet.2025.107122","DOIUrl":"10.1016/j.mimet.2025.107122","url":null,"abstract":"<div><div>Phenotypic typing methods are often time consuming and do not adequately discriminate among the strains involved. Innovative molecular techniques can perform direct typing by analyzing DNA and are becoming widespread as important tools for bacterial typing, molecular epidemiology and molecular systematization. The aim of this study was to genotype <em>Corynebacterium pseudotuberculosis</em> strains using PCR-based DNA fingerprinting methods and to evaluate the methods comparatively. Within the scope of the study, 17 <em>C. pseudotuberculosis</em> strains were analyzed. The strains were genotyped by enterobacterial repetitive intergenic consensus (ERIC)-PCR using ERIC2 primer; by random amplified polymorphic (RAPD) DNA-PCR using primers P5, P6, P11, P14, P16, P21 and M13; and by (GTG)5-PCR using (GTG)5 primer. The discrimination power and confidence intervals of the methods were calculated based on the genotyping results using each primer. All strains produced amplification products with the primers used for genotyping. As a result of genotyping with ERIC2, P14, P11, (GTG)5, P21, P5, M13, P6 and P16 primers, the discrimination powers (confidence intervals) were calculated as 0.8603(0.858–0.862), 0.7132(0.709–0.716), 0.6838(0.668–0.699), 0.6397(0.623–0.656), 0.5809(0.560–0.601), 0.3235(0.293–0.353), 0.1176(0.081–0.153), 0.1176(0.081–0.153) and 0.1176(0.081–0.153), respectively. As a result of the comparative evaluation of the results, it was observed that ERIC2 and P14 primers had high discrimination power and confidence interval in genotyping <em>C. pseudotuberculosis</em> strains, while M13, P6 and P16 primers were insufficient in genotyping of strains. It was concluded that genotyping with ERIC2 and P14 primers can be used reliably in investigating the molecular epidemiology of infections and/or outbreaks caused by <em>C. pseudotuberculosis</em>.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"232 ","pages":"Article 107122"},"PeriodicalIF":1.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}