Journal of microbiological methods最新文献_第6页

Corrigendum to “Application of flow cytometry for rapid, high-throughput, multiparametric analysis of environmental microbiomes” [Journal of Microbiological Methods 214 (2023) 106841] 应用流式细胞仪对环境微生物组进行快速、高通量、多参数分析》[《微生物学方法杂志》214 (2023) 106841]更正。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-30 DOI: 10.1016/j.mimet.2024.107005

Madhumita Priyadarsini, Jeetesh Kushwaha, Kailash Pati Pandey, Jyoti Rani, Abhishek S. Dhoble

引用次数: 0

Developing fish oil emulsion gel enriched with Lentinula edodes single cell protein and its effect on controlling the growth of Acinetobacter baumannii 开发富含小扁豆单细胞蛋白的鱼油乳液凝胶及其对鲍曼不动杆菌生长的控制效果。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-26 DOI: 10.1016/j.mimet.2024.107006

Elif Erdogan Eliuz, Deniz Ayas

{"title":"Developing fish oil emulsion gel enriched with Lentinula edodes single cell protein and its effect on controlling the growth of Acinetobacter baumannii","authors":"Elif Erdogan Eliuz, Deniz Ayas","doi":"10.1016/j.mimet.2024.107006","DOIUrl":"10.1016/j.mimet.2024.107006","url":null,"abstract":"<div><p>In this study, the characterization of fish oil (FO) emulsion gel (EGEL) containing single cell protein (SCP) produced from <em>Lentinula edodes</em> (<em>L. edodes</em>) and its potential inhibition against <em>Acinetobacter baumannii</em> (<em>A. baumannii</em>) were investigated. Oil extracted from the fish liver was emulsified with tween 80 and water, and then gelled using gelatin with the assistance of an ultrasonic homogenizer. The characteristics and surface analysis of SCP-EGEL were examined using FTIR (Fourier-transform infrared spectroscopy) and SEM (Scanning electron microscope). The particle size distribution and zeta potential of SCP-EGEL were measured using a Malvern Zetasizer. When SCP-EGEL was applied to the surface of the medium inoculated with <em>A. baumannii</em>, the inhibition zone (IZ) was 8.2 mm. An expansion of the IZ was observed (10.2 mm) when SCP-EGEL was applied to a fish skin (FS) surface prepared in the shape of a 6-mm diameter disc. In the SEM images, when SCP was added to lipo gel, the gel structure appeared flattened or swollen in some areas. The appearance of SCP cells being covered with gel gave the impression that they have a secondary wall. Therefore, the resulting complex can potentially be used as an additive in animal and human nutrition, in functional food coatings to suppress <em>A. baumannii</em>, and in fish feed to enrich it with protein.</p></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"224 ","pages":"Article 107006"},"PeriodicalIF":1.7,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the microbiological treatment of sperm for assisted reproductive technology (ART) 优化用于辅助生殖技术（ART）的精子微生物处理。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-22 DOI: 10.1016/j.mimet.2024.107004

Affoua-Melissa Bekon , Delphine Martiny , Anne-Laure Thomas , Yvette Miendje , Anne-Laurence Debyttere , Candice Autin , Evelyne Bertrand

引用次数: 0

Evaluation of Vitek-2 and MicroScan ASTs directly from positive blood culture: A cost-effective 24-h reduction for Enterobacterales and staphylococci 评估直接从阳性血培养物中提取的 Vitek-2 和 MicroScan AST：具有成本效益的 24 小时肠杆菌和葡萄球菌减毒法。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-20 DOI: 10.1016/j.mimet.2024.107003

M. Peradotto , L. Pangaro , C. Tavano , A. Ettori , A. Frigeri , E. Verri , G. Caffiero , L. Soattini , S. Daffara , L. Cianci , M. Pelagi

{"title":"Evaluation of Vitek-2 and MicroScan ASTs directly from positive blood culture: A cost-effective 24-h reduction for Enterobacterales and staphylococci","authors":"M. Peradotto , L. Pangaro , C. Tavano , A. Ettori , A. Frigeri , E. Verri , G. Caffiero , L. Soattini , S. Daffara , L. Cianci , M. Pelagi","doi":"10.1016/j.mimet.2024.107003","DOIUrl":"10.1016/j.mimet.2024.107003","url":null,"abstract":"<div><p>The reduction of antimicrobial susceptibility testing (AST) time-to-result is a central need, especially in sepsis treatment. The current automated rapid ASTs are still too expensive for many laboratories.</p><p>We aimed to evaluate three pre-treatment methods for a same-day inoculation on both automated AST platforms available in our laboratory. We tested 100 <em>Enterobacterales</em> or staphylococci positive bottles.</p><p>We obtained good results with the different methods and instruments. In particular, Vitek-2 showed good performances with <em>Enterobacterales</em> AST when inoculated with bacterial pellet (96.6% categorical agreement - CA-, 93.3% essential agreement - EA). Also short-term incubation colonies for staphylococci AST had acceptable CA (94.2%), even if with 77.5% EA. MicroScan system for staphylococci AST with both short-term incubation and direct blood inoculation reached >95% CA, but 92.5% and 83.6% EA, respectively. On the other hand, <em>Enterobacterales</em> AST showed optimal performances only with bacterial pellet inoculation (97.6% CA). In fact, direct blood inoculation showed not acceptable parameters for several molecules.</p><p>Both systems allow a 24-h reduction in time-to-result, by using the same instruments of routine activity after rapid and cheap pre-treatments.</p></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"224 ","pages":"Article 107003"},"PeriodicalIF":1.7,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of bactericidal activity against 3HC-2-Tre-labelled Mycobacterium abscessus (Mycobacteroides abscessus) by automated fluorescence microscopy 利用自动荧光显微镜测定对 3HC-2-Tre 标记的脓肿分枝杆菌（脓肿分枝杆菌）的杀菌活性。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-20 DOI: 10.1016/j.mimet.2024.107002

Lea Mann, Fabienne Siersleben, Markus Lang, Adrian Richter

{"title":"Determination of bactericidal activity against 3HC-2-Tre-labelled Mycobacterium abscessus (Mycobacteroides abscessus) by automated fluorescence microscopy","authors":"Lea Mann, Fabienne Siersleben, Markus Lang, Adrian Richter","doi":"10.1016/j.mimet.2024.107002","DOIUrl":"10.1016/j.mimet.2024.107002","url":null,"abstract":"<div><p>The minimum bactericidal concentration (MBC) of antibiotics is an important parameter for the potency of a drug in eradicating a bacterium as well as an important measure of the potential of a drug candidate in research and development. We have established a fluorescence-based microscopy method for the determination of MBCs against the non-tuberculous mycobacterium <em>Mycobacterium abscessus</em> (<em>Mycobacteroides abscessus</em>) to simplify and accelerate the performance of MBC determination compared to counting colony forming units on agar. Bacteria are labelled with the trehalose-coupled dye 3HC-2-Tre and analysed in a 96-well plate. The results of the new method are consistent with MBC determination by plating on agar. The method was used to evaluate the bactericidality of the antibiotics rifabutin, moxifloxacin, amikacin, clarithromycin and bedaquiline. Bactericidal effects against <em>M. abscessus</em> were observed, which are consistent with literature data.</p></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"224 ","pages":"Article 107002"},"PeriodicalIF":1.7,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0167701224001143/pdfft?md5=adc467c1a1ccd94ba64d89ee8d3a80c7&pid=1-s2.0-S0167701224001143-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step cloning and targeted duplication of Pantoea ananatis chromosomal fragments 盘尾丝菌染色体片段的一步克隆和定向复制。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-19 DOI: 10.1016/j.mimet.2024.106999

O. Igonina, V. Samsonov, N. Stoynova

引用次数: 0

Adapted molecular methods to unravel the recalcitrant mycorrhizal associations of Aucoumea klaineana Pierre 采用分子方法揭示 Aucoumea klaineana Pierre 的顽固菌根关系。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-17 DOI: 10.1016/j.mimet.2024.107000

Quentin Guidosse , Mélanie Roy , Ludivine Lassois , Jean-Louis Doucet

引用次数: 0

Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH). 利用荧光原位杂交（FISH）技术观察培养细胞、受感染绵羊和人体组织切片中的副结核分枝杆菌。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-17 DOI: 10.1016/j.mimet.2024.107001

Neil Rayment , Glenn Rhodes , Barry Hudspith , Valerie Hughes , Francesca Chianini , Gaurav Agrawal , Tim J. Bull , Roger Pickup , Jeremy Sanderson

引用次数: 0

Leveraging artificial intelligence in vaccine development: A narrative review 利用人工智能开发疫苗：叙述性综述。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-15 DOI: 10.1016/j.mimet.2024.106998

David B. Olawade , Jennifer Teke , Oluwaseun Fapohunda , Kusal Weerasinghe , Sunday O. Usman , Abimbola O. Ige , Aanuoluwapo Clement David-Olawade

{"title":"Leveraging artificial intelligence in vaccine development: A narrative review","authors":"David B. Olawade , Jennifer Teke , Oluwaseun Fapohunda , Kusal Weerasinghe , Sunday O. Usman , Abimbola O. Ige , Aanuoluwapo Clement David-Olawade","doi":"10.1016/j.mimet.2024.106998","DOIUrl":"10.1016/j.mimet.2024.106998","url":null,"abstract":"<div><p>Vaccine development stands as a cornerstone of public health efforts, pivotal in curbing infectious diseases and reducing global morbidity and mortality. However, traditional vaccine development methods are often time-consuming, costly, and inefficient. The advent of artificial intelligence (AI) has ushered in a new era in vaccine design, offering unprecedented opportunities to expedite the process. This narrative review explores the role of AI in vaccine development, focusing on antigen selection, epitope prediction, adjuvant identification, and optimization strategies. AI algorithms, including machine learning and deep learning, leverage genomic data, protein structures, and immune system interactions to predict antigenic epitopes, assess immunogenicity, and prioritize antigens for experimentation. Furthermore, AI-driven approaches facilitate the rational design of immunogens and the identification of novel adjuvant candidates with optimal safety and efficacy profiles. Challenges such as data heterogeneity, model interpretability, and regulatory considerations must be addressed to realize the full potential of AI in vaccine development. Integrating emerging technologies, such as single-cell omics and synthetic biology, promises to enhance vaccine design precision and scalability. This review underscores the transformative impact of AI on vaccine development and highlights the need for interdisciplinary collaborations and regulatory harmonization to accelerate the delivery of safe and effective vaccines against infectious diseases.</p></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"224 ","pages":"Article 106998"},"PeriodicalIF":1.7,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0167701224001106/pdfft?md5=88534f3ed87550722c7b69698732008d&pid=1-s2.0-S0167701224001106-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector 在廉价的锥形平底聚丙烯管中培养的大肠杆菌能产生大量 pUC 载体。

IF 1.7 4区生物学

Journal of microbiological methods Pub Date : 2024-07-14 DOI: 10.1016/j.mimet.2024.106990

Mayra Marquez, Qian Chen, Silvia Cachaco, Jun Yang, Hongyan Sui, Tomozumi Imamichi

引用次数: 0