Journal of microbiological methods最新文献

{"title":"Comparison of light scattering signals vs BODIPY™ 493/503 staining in flow cytometry for the quantitative monitoring of P(3HB-co-3 HV) biosynthesis.","authors":"Coline Perdrier, Lucie Farrera, Maeva Subileau, Laurence Preziosi-Belloy, Estelle Grousseau","doi":"10.1016/j.mimet.2026.107532","DOIUrl":"https://doi.org/10.1016/j.mimet.2026.107532","url":null,"abstract":"<p><p>Analysis of microbial cultures traditionally relies on average population characteristics, despite the inherent variability of individual cells. This variability affects process performance and makes bioprocesses scale-up challenging. Flow cytometry, a multi-parametric and near-line method provides information at cellular level. This study evaluated the potential of light scattering signals versus BODIPY™ 493/503 staining in flow cytometry for the quantitative monitoring of P(3HB-co-3 HV) biosynthesis by Cupriavidus necator. A dedicated bioreactor culture was performed to generate a dataset with samples presenting a wide range of P(3HB-co-3 HV) contents (6 to 70 wt%). The relationships were then validated using data from two independent cultures. For cells with levels ≥0.06 pg_PHA.cell^-1 (30 wt%) the FSC light scattering signal proved to be a simple fast and accurate method for monitoring phases of P(3HB-co-3 HV) accumulation without the need for staining. For early growth phases or low PHA contents (< 0.06 pg_PHA.cell^-1), BODIPY™ staining remains necessary to identify and quantify PHA-producing cells. Monitoring of sub-population dynamics during PHA production showed an increase in the diversity of cellular PHA content over time, with end-of-culture levels ranging from <0.01 pg_PHA.cell^-1 to over 0.86 pg_PHA.cell^-1, with damaged cells found mainly in subpopulations with low PHA content.</p>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":" ","pages":"107532"},"PeriodicalIF":1.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of selective enrichment media for accurate most probable number enumeration of Listeria monocytogenes in ready-to-eat seafood products.","authors":"Kaori Komori, Yuna Kono, Ayaka Nakamura, Takashi Kuda, Hajime Takahashi","doi":"10.1016/j.mimet.2026.107527","DOIUrl":"https://doi.org/10.1016/j.mimet.2026.107527","url":null,"abstract":"<p><p>Listeria monocytogenes is a foodborne bacterium that can cause listeriosis. Ready-to-eat (RTE) seafood products are among the food categories associated with listeriosis. Because contamination levels of L. monocytogenes in RTE seafood products are often below 10 CFU/g, accurate enumeration of low bacterial concentrations is essential for reliable risk assessment of these products. A most probable number (MPN) method is widely used for enumerating low bacterial concentrations in food samples; however, the choice of selective enrichment medium strongly affects recovery, especially in the presence of competing microflora and injured cells. In this study, five types of RTE seafood products were inoculated with low concentrations of L. monocytogenes, and four selective enrichment media including half Fraser broth, Listeria enrichment broth (UVM and FDA formulation), and buffered Listeria enrichment broth (BLEB) were compared. When samples were incubated in BLEB for 48 h, the MPN values agreed with the inoculation levels in 93-100% across strains and food matrices. Under these conditions, MPN estimates for heat-injured inocula were accurate in 93% of cases. Among the media evaluated, incubation in BLEB for 48 h yielded the most accurate and consistent MPN estimates across strains and food matrices, supporting its suitability for quantitative analysis of low levels of L. monocytogenes.</p>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":" ","pages":"107527"},"PeriodicalIF":1.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized protocol for high-efficiency mitochondrial RNA isolation from fission yeast in log phase and stationary phase.","authors":"Yijing Lu, Gang Wang, Jinjie Shang","doi":"10.1016/j.mimet.2026.107523","DOIUrl":"https://doi.org/10.1016/j.mimet.2026.107523","url":null,"abstract":"<p><p>The fission yeast Schizosaccharomyces pombe (S. pombe) serves as an important model organism for investigating mitochondrial function and gene regulation. However, obtaining sufficient quantity and high-quality mitochondrial RNA (mtRNA) for techniques like Northern blot analysis remains challenging, particularly from stationary-phase cells with rigid cell walls. We developed an improved extraction method by comparing conventional hot-phenol and commercial column-based approaches with modified Enzymatic-Phenol/Chloroform (EPC) protocol, which replaces harsh thermal/liquid nitrogen steps with gentle enzymatic lysis. Additionally, for hard-to-lyse stationary-phase cells, we introduced a specialized OM Buffer to enhance efficiency. The refined EPC protocol achieved substantially higher mtRNA yields without compromising RNA purity. Northern blot analysis confirmed successful isolation of mtRNAs, revealing strong, well-defined signals for mtRNA. This protocol provides a reliable tool for advanced mitochondrial research in S. pombe and other eukaryotes.</p>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":" ","pages":"107523"},"PeriodicalIF":1.9,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species-dependent colony size variation of environmental Enterococcus on sodium azide-containing selective agar.","authors":"Sze Chin Lim, Ayaka Nakamura, Takashi Kuda, Hajime Takahashi","doi":"10.1016/j.mimet.2026.107525","DOIUrl":"https://doi.org/10.1016/j.mimet.2026.107525","url":null,"abstract":"<p><p>The gold standard for bacterial isolation is the selection of individual colonies from agar media. Selective media supplemented with chemical inhibitors are used to promote the growth of target bacteria while suppressing non-target organisms, particularly in environmental samples. Enterococcus, a genus comprising multiple species commonly found in bacteria-rich environments, can be isolated using agar supplemented with sodium azide. However, this approach generally enables identification only at the genus level, with limited discrimination among Enterococcus species. This study provides a detailed characterization of colony morphology in enterococci isolated from surface water using colony diameter as a discriminating parameter. At 0.4 g/L sodium azide, E. casseliflavus formed significantly smaller colonies (0.7 mm) than other Enterococcus species (>1.0 mm). Species forming colonies exceeding 1.0 mm were further characterized based on their extracellular polymeric substances (EPS). FTIR analysis of crude EPS (50-100 kDa) extracted by centrifugal ultrafiltration revealed similar spectral profiles among large-diameter E. hirae, E. lactis, and E. faecium, distinguishing them from E. faecalis. This study demonstrates that colony morphology on selective media can serve as a practical, culture-based approach for preliminary differentiation of selected Enterococcus species.</p>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":" ","pages":"107525"},"PeriodicalIF":1.9,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From clinics to the environment: A systematic review of machine learning and MALDI-TOF MS in the identification of antimicrobial-resistant bacteria","authors":"Nafyad Ibrahim Batu,&nbsp;Ilunga Kamika,&nbsp;Tshepo Joseph Malefetse","doi":"10.1016/j.mimet.2026.107414","DOIUrl":"10.1016/j.mimet.2026.107414","url":null,"abstract":"<div><div>As a result of the increasing prevalence of Antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in both community and hospital settings, their identification by conventional approaches has been posing a significant issue for decades. A new approach, integrating matrix-assisted laser desorption/ionization time of-flight mass spectrometry (MALDI-TOF-MS) with machine learning (ML), has emerged as a valuable method for their identification. This review systematically evaluates the effectiveness of integrating MALDI-TOF-MS with ML for the identification of ARB. A comprehensive literature search was conducted using PubMed, Google Scholar, the Cochrane Library, Scopus, and Web of Science to identify original research articles focused on the application of MALDI-TOF-MS and ML in detecting ARB. Studies unrelated to bacteria or antibiotic resistance, as well as short communications, scientific reports, and case studies, were excluded. In accordance with PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines, 401 potentially relevant articles were initially identified. Following the application of inclusion criteria and relevance assessment of titles and abstracts, 34 studies were selected for final analysis. The findings demonstrate that integrating MALDI-TOF-MS with ML models markedly improves the speed, accuracy, and reliability of ARB detection while offering valuable insights into the molecular mechanisms of resistance. Current evidence suggests that the integration of MALDI TOF MS with ML is an important approach for the identification of bacteria in clinics and environments, mainly ARB. Future research is needed to apply this approach to address the growing challenge of ARB both in clinical and environmental settings.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"243 ","pages":"Article 107414"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a multiplex PCR assay for rapid detection of Clostridium perfringens, Clostridium septicum and Paeniclostridium sordellii in necrotising soft tissue infections","authors":"Anjali Anil ,&nbsp;Tanvi Vashist ,&nbsp;Divya Nair ,&nbsp;Kamlesh Bisht ,&nbsp;Pallab Ray ,&nbsp;Cherring Tandup ,&nbsp;Karthick Rangasamy ,&nbsp;Archana Angrup","doi":"10.1016/j.mimet.2026.107420","DOIUrl":"10.1016/j.mimet.2026.107420","url":null,"abstract":"<div><h3><u>Background</u></h3><div>Necrotising soft tissue infections (NSTIs) are fulminant infections requiring rapid diagnosis and intervention. Conventional methods, such as anaerobic culture, are slow and insensitive for clostridial pathogens.</div></div><div><h3>Objectives</h3><div>To develop and evaluate a multiplex PCR assay targeting three clinically relevant clostridia directly in NSTI samples.</div></div><div><h3>Methods</h3><div>A multiplex conventional PCR targeting the <em>cpa</em> (<em>Clostridium perfringens</em>), <em>csa</em> (<em>Clostridium septicum</em>), and 16S rRNA (<em>Paeniclostridium sordellii</em>) genes was optimised and applied to tissue/pus samples from 87 clinically diagnosed NSTI patients. Anaerobic culture served as the reference. Sensitivity, specificity, and diagnostic turnaround times were compared.</div></div><div><h3>Results</h3><div>Seven patients (8.0%, 95% CI 3.3–16.2) were positive for clostridia by PCR, compared with four (4.6%, 95% CI 1.3–11.4) by culture. PCR identified three additional culture-negative cases. While McNemar's test did not show statistical significance (<em>p</em> = 0.25), PCR achieved a sensitivity of 100% (95% CI 39.8–100.0), specificity of 96.4% (95% CI 89.8–99.2), and a negative predictive value of 100%. Turnaround time was 3–4 h, compared with 48–96 h for culture. Both patients with monomicrobial <em>C. septicum</em> NSTI succumbed rapidly, highlighting its prognostic importance.</div></div><div><h3>Conclusion</h3><div>This is the first report of a multiplex PCR assay for direct detection of <em>C. perfringens, C. septicum,</em> and <em>P. sordellii</em> in NSTIs. The assay enhances detection, reduces turnaround time, and provides clinically relevant species-level identification. Multiplex PCR may serve as a valuable adjunct to culture in early NSTI diagnostics and could be expanded to include additional anaerobic pathogens.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"243 ","pages":"Article 107420"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146157375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of bacteria-specific odor signatures using low-cost metal oxide gas sensors","authors":"Emre Yavuzer ,&nbsp;Mahmut Yılmaz","doi":"10.1016/j.mimet.2026.107429","DOIUrl":"10.1016/j.mimet.2026.107429","url":null,"abstract":"<div><div>In this study, a feasibility assessment was conducted to evaluate the potential of commercially available low-cost gas sensors for differentiating bacterial species inoculated into fish muscles. The signals were obtained by determining how low-cost gas sensors detected the volatile organic compound (VOC) patterns produced by each bacterial species (<em>Enterococcus faecalis, Pseudomonas luteola, Proteus mirabilis</em>, and <em>Photobacterium damselae</em>) in trout tissue. It was observed that MQ3 and MQ4 sensors yielded high rates for all bacteria, indicating a strong increase in broad VOC load consistent with the known cross-sensitivity range of MQ sensors as a result of bacterial metabolism. In addition to cumulative VOC patterns, time-dependent derivative analysis (dVOC/dt) was applied to reveal bacterium-specific metabolic differences in more detail. Thus, it was determined that <em>Photobacterium damselae</em>, unlike other bacteria, exhibited suppressed VOC production kinetics in the early stages, demonstrating that dynamic VOC fingerprints are a powerful tool for bacterial differentiation. The results demonstrate the feasibility of dynamic VOC fingerprinting using minimal sensor configurations for exploratory bacterial discrimination.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"243 ","pages":"Article 107429"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146147466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KguS/KguR two-component system as a molecular marker for E. coli strains associated with chronic urinary tract infections (cUTI)","authors":"Ricardo E. Ahumada-Cota ,&nbsp;Ulises Hernández-Chiñas ,&nbsp;Brenda Recillas-Farfán ,&nbsp;Armando Navarro-Ocaña ,&nbsp;José Molina-López ,&nbsp;María G. Balbuena-Alonso ,&nbsp;María E. Chávez-Berrocal ,&nbsp;Carlos A. Eslava-Campos","doi":"10.1016/j.mimet.2026.107421","DOIUrl":"10.1016/j.mimet.2026.107421","url":null,"abstract":"<div><div>The identification of key genes responsible for the colonization and infection of <em>E. coli</em> strains associated with diarrhea (DEC) has made significant progress. Unfortunately, for uropathogenic <em>E. coli</em> (UPEC) strains, there are currently no defined genes that contribute to the identification of strains associated with urinary tract infections. This paper presents data regarding genes associated with the virulence and adaptability of UPEC strains isolated from urinary tract infections (UTI), aiming to evaluate the possibility of identifying a molecular marker for this pathotype. We analyzed 166 <em>E. coli</em> strains (90 recovered from urine and 76 isolated from feces) characterized by serotyping, phylogenetic analysis, and PCR detection of <em>fimH</em>, <em>iutA</em>, <em>fyuA</em>, <em>feoB</em>, <em>ompT</em>, <em>kguS</em> and <em>kguR</em>, genes associated with the virulence and metabolism of UPEC. Herein, results showed a higher prevalence of <em>kguS/kguR</em> combination in strains isolated from persistent UTI (peUTI), which correlated with strains of classic UPEC serogroups, a higher virulence gene load, and inclusion in pathogenic phylogroups (B2 and D). The presence of the genes in strains isolated from feces showed that the <em>kguS/kguR</em> presence was minimal (14%), while the presence of <em>kguR</em> was almost inexistent (1%), contrary to <em>kguS</em> (39%). The results obtained suggest that the presence of the <em>kguS/kguR</em> pair encoding the homonymous two-component system (TCS) plays an important role in the adaptation and colonization of the urinary tract by <em>E. coli</em> strains that cause UTI. In conclusion, the combination of <em>kguS/kguR</em> could be used as a molecular marker to identify pathogenic strains with a high capacity for adaptation to the urinary environment.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"243 ","pages":"Article 107421"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of semi-dry wine using cape gooseberry under very high gravity conditions with preserved antioxidant properties","authors":"Somil Kumar ,&nbsp;Naveen Malik ,&nbsp;Akhilesh Kumar ,&nbsp;Sunil Kumar ,&nbsp;Vikram Kumar ,&nbsp;Umesh kumar Dwivedi ,&nbsp;Rajesh Sharma ,&nbsp;Ajay Bhardwaj ,&nbsp;Sudarshan Singh Lakhawat ,&nbsp;Pushpender Kumar Sharma","doi":"10.1016/j.mimet.2026.107418","DOIUrl":"10.1016/j.mimet.2026.107418","url":null,"abstract":"<div><div>In this study, we have reported production of a semi-dry fruit wine using <em>Cape Gooseberry</em> for the first time using very high gravity conditions at laboratory scale. Employing industrial strain <em>Saccharomyces cerevisiae,</em> the batch fermentation was carried out at room temperature in 1-l flasks in triplicates. The moisture content of fruit was calculated to be 80% after 24 days. The °Bx of raw fruit juice was found to be 4.9 and was raised to 25°Bx on addition of sucrose, the yeast assimilable nitrogen content of cape goose berry juice was calculated to be 280 mg N/L. Average fermentation time calculated based on cessation of bubbling and attainment of final °Bx of 3.8 ± 0.3 was found to be 21 days. The initial pH of raw juice was observed to be 4.51 ± 0.04 before fermentation, while post fermentation it was calculated to be 3.63 ± 0.03. The ethanol estimated using HPLC, GC-FID and refractometric analysis showed concentration of 13.29 ± 0.26, 13.15 ± 0.03 and 13.63 ± 0.374% respectively. The antioxidant activity of raw juice vs processed cape gooseberry wine was calculated to be 65.06% and 39.64% respectively, while it was estimated to be 53.86% in one-year-old preserved cape gooseberry wine sample. The titratable acidity and volatile acidity of wine was determined to be 6 g/l and 0.15 g/l respectively and was found to be well within the desired range. Our results provide a clear demonstration of feasibility of very high gravity fermentation for producing semi-dry Cape Gooseberry wine with moderate antioxidant preservation.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"243 ","pages":"Article 107418"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization and Comparative Evaluation of quick Inoculation Techniques for Screening Pigeonpea genotype Resistance to Phytophthora cajani","authors":"Pragati Nema ,&nbsp;Raj K. Mishra ,&nbsp;Sonika Pandey ,&nbsp;Rajani Sasode","doi":"10.1016/j.mimet.2026.107419","DOIUrl":"10.1016/j.mimet.2026.107419","url":null,"abstract":"<div><div>Phytophthora stem blight (PSB), caused by <em>Phytophthora cajani</em>, is a destructive disease of pigeonpea (<em>Cajanus cajan</em> L.) that can lead to complete crop loss under favorable conditions. Effective resistance breeding is constrained by the lack of reliable and reproducible screening methods. The present study aimed to standardize and compare artificial inoculation techniques for consistent induction of Phytophthora blight under controlled conditions. Ten inoculation methods targeting different infection pathways were evaluated in greenhouse pot experiments over two consecutive seasons (2023–24 and 2024–25) using susceptible (UPAS-120, ICP-7119, ICP-2376) and moderately resistant (KPBR-80-2-1, IPAC-79, IPAB-7-2-1-7) pigeonpea cultivars. Disease incidence was recorded to assess the efficiency and reproducibility of each technique. All methods successfully established infection; however, disease severity varied significantly among techniques and genotypes. The leaf-lamina inoculation method consistently produced the highest and most uniform disease incidence, recording 97.7% and 96.6% in the susceptible cultivar UPAS-120 and 83.3% and 86.6% in the moderately resistant cultivar KPBR-80-2-1 across the two seasons. Node inoculation emerged as the second most reliable method. The study identifies most efficient and quick, reproducible inoculation techniques that enable rapid and high-throughput resistance screening, providing a robust methodological framework to support pigeonpea breeding and Phytophthora blight management.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"243 ","pages":"Article 107419"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}